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The map label for this gene is ooxB [H]

Identifier: 138896857

GI number: 138896857

Start: 3330853

End: 3331926

Strand: Reverse

Name: ooxB [H]

Synonym: GTNG_3220

Alternate gene names: 138896857

Gene position: 3331926-3330853 (Counterclockwise)

Preceding gene: 138896858

Following gene: 138896856

Centisome position: 93.85

GC content: 38.55

Gene sequence:

>1074_bases
ATGGAAACGAACTACGAAACGATAATTATTGGTGGCGGGGTTATTGGTAATAGTATTGCTTATCATCTCTCGGAGCGGTA
TAAAGGGGGAATATTAGTAATTGATAAGAAGTTCCCAATGTCAGGGACTTCAGGAGCTACACAAGCCTGGGTATGGGTGC
ATAATAAAACTCCTGCTTGGTATGCAGAATTTAGTATGTATAGTGCAGAGCTTTATTCATATCTATCAAAAAAAATTGGG
GATGTTGAGTATAGACGAACAGGAGGATTATCTCCTTTTTTCACTGTGAGAGATTTGGAAAAGGCTAAGCGGCTTGCTGA
TTCTTATGAAAAAATTGGAATAAAAATTAAAGTTTTATCACGGGAAGAGGTATTAGAAAAGGAACCTTTTATTAATCCTA
AAGTTGTGGGGGCAACCTTTAGTCGAATTGACGGGAACGTTAATCCATTCCGGTTAATTGACATGTATATGAGGGCGGCT
AAAAAAAATGGTGTTCACTATTCCACTTATAATCGAGTAATAGACATTCAAAAACAGAACGGTCAGTTCGTTGTTATTTC
TGAAAAAACAACGTATGTTACAAAAAACCTTATTCTCGCAGGTGGCCCTTGGTCAAGGGAACTGGGGAGAATGGTAGGTG
TCGATATACCTGTTAATCAAGTGAGAGGACAAATTCTTGTTTCAGAACCATTAAAGCCTTTTTTAAACTATACGATAAGT
GGATTAAGGCAAACCAATAATGGTGAAGTATTGATAGGTTATTCTATGGAGCAGGCGGGTTTTGACCGATCCACTACACT
TGACGTTATTCAAGAAACTGCCAATATGGCTGTCCATTACGTTCCTTCGTTGAGAAAAGCGAAAATTGTGCGTACTTTTT
CAGGAATTCGCGCTATGCCGAAGGATGGTTTACCGATTATTGGAAGAGTTCCTGATGTTGAGAATCTTTACGTAGCGGCT
ACACATAGTGGTATAACACTTTCCCCTTTAATAGGGACGATCATGACCGAGTTAATTGTAGACGGAGAAACATCTATTCC
GATTGACCGATATTCAATTACAAGATTTTCTTGA

Upstream 100 bases:

>100_bases
CTCCTATAAAGCTTGAAACGTTAGCCACTAATAGCACTTCGTTTTATGCAGTAAAATCCGTTTTGGATGAGTTGGAGTTA
GAGGAGAGGAGATAGAATAA

Downstream 100 bases:

>100_bases
AAAGGGGAGTTCATTATCTGTTCCTAAATGCAAACCAATCGGCCTTTTCCCTAATAAGTAATGGGAAAAGGCCATGATTT
TGCAGGTGATTTTAGGATGA

Product: sarcosine oxidase subunit beta

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Octopine oxidase subunit B [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
METNYETIIIGGGVIGNSIAYHLSERYKGGILVIDKKFPMSGTSGATQAWVWVHNKTPAWYAEFSMYSAELYSYLSKKIG
DVEYRRTGGLSPFFTVRDLEKAKRLADSYEKIGIKIKVLSREEVLEKEPFINPKVVGATFSRIDGNVNPFRLIDMYMRAA
KKNGVHYSTYNRVIDIQKQNGQFVVISEKTTYVTKNLILAGGPWSRELGRMVGVDIPVNQVRGQILVSEPLKPFLNYTIS
GLRQTNNGEVLIGYSMEQAGFDRSTTLDVIQETANMAVHYVPSLRKAKIVRTFSGIRAMPKDGLPIIGRVPDVENLYVAA
THSGITLSPLIGTIMTELIVDGETSIPIDRYSITRFS

Sequences:

>Translated_357_residues
METNYETIIIGGGVIGNSIAYHLSERYKGGILVIDKKFPMSGTSGATQAWVWVHNKTPAWYAEFSMYSAELYSYLSKKIG
DVEYRRTGGLSPFFTVRDLEKAKRLADSYEKIGIKIKVLSREEVLEKEPFINPKVVGATFSRIDGNVNPFRLIDMYMRAA
KKNGVHYSTYNRVIDIQKQNGQFVVISEKTTYVTKNLILAGGPWSRELGRMVGVDIPVNQVRGQILVSEPLKPFLNYTIS
GLRQTNNGEVLIGYSMEQAGFDRSTTLDVIQETANMAVHYVPSLRKAKIVRTFSGIRAMPKDGLPIIGRVPDVENLYVAA
THSGITLSPLIGTIMTELIVDGETSIPIDRYSITRFS
>Mature_357_residues
METNYETIIIGGGVIGNSIAYHLSERYKGGILVIDKKFPMSGTSGATQAWVWVHNKTPAWYAEFSMYSAELYSYLSKKIG
DVEYRRTGGLSPFFTVRDLEKAKRLADSYEKIGIKIKVLSREEVLEKEPFINPKVVGATFSRIDGNVNPFRLIDMYMRAA
KKNGVHYSTYNRVIDIQKQNGQFVVISEKTTYVTKNLILAGGPWSRELGRMVGVDIPVNQVRGQILVSEPLKPFLNYTIS
GLRQTNNGEVLIGYSMEQAGFDRSTTLDVIQETANMAVHYVPSLRKAKIVRTFSGIRAMPKDGLPIIGRVPDVENLYVAA
THSGITLSPLIGTIMTELIVDGETSIPIDRYSITRFS

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI24797151, Length=386, Percent_Identity=24.0932642487047, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI197927446, Length=383, Percent_Identity=20.6266318537859, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI21361378, Length=383, Percent_Identity=20.6266318537859, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI194306651, Length=389, Percent_Identity=22.3650385604113, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71994045, Length=383, Percent_Identity=25.8485639686684, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71994052, Length=390, Percent_Identity=25.6410256410256, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571104, Length=384, Percent_Identity=22.9166666666667, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 39880; Mature: 39880

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METNYETIIIGGGVIGNSIAYHLSERYKGGILVIDKKFPMSGTSGATQAWVWVHNKTPAW
CCCCCEEEEEECCHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCE
YAEFSMYSAELYSYLSKKIGDVEYRRTGGLSPFFTVRDLEKAKRLADSYEKIGIKIKVLS
EEHHHHHHHHHHHHHHHHHCCEEEHHCCCCCCEEEHHHHHHHHHHHHHHHHCCEEEEEEC
REEVLEKEPFINPKVVGATFSRIDGNVNPFRLIDMYMRAAKKNGVHYSTYNRVIDIQKQN
HHHHHHCCCCCCCEEECCCHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCEEEEEEECC
GQFVVISEKTTYVTKNLILAGGPWSRELGRMVGVDIPVNQVRGQILVSEPLKPFLNYTIS
CCEEEEECCCEEEEEEEEEECCCCHHHHHHHCCCCCCHHHHCCEEEECCCHHHHHHHHHH
GLRQTNNGEVLIGYSMEQAGFDRSTTLDVIQETANMAVHYVPSLRKAKIVRTFSGIRAMP
HHEECCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHCCCCC
KDGLPIIGRVPDVENLYVAATHSGITLSPLIGTIMTELIVDGETSIPIDRYSITRFS
CCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
METNYETIIIGGGVIGNSIAYHLSERYKGGILVIDKKFPMSGTSGATQAWVWVHNKTPAW
CCCCCEEEEEECCHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCE
YAEFSMYSAELYSYLSKKIGDVEYRRTGGLSPFFTVRDLEKAKRLADSYEKIGIKIKVLS
EEHHHHHHHHHHHHHHHHHCCEEEHHCCCCCCEEEHHHHHHHHHHHHHHHHCCEEEEEEC
REEVLEKEPFINPKVVGATFSRIDGNVNPFRLIDMYMRAAKKNGVHYSTYNRVIDIQKQN
HHHHHHCCCCCCCEEECCCHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCEEEEEEECC
GQFVVISEKTTYVTKNLILAGGPWSRELGRMVGVDIPVNQVRGQILVSEPLKPFLNYTIS
CCEEEEECCCEEEEEEEEEECCCCHHHHHHHCCCCCCHHHHCCEEEECCCHHHHHHHHHH
GLRQTNNGEVLIGYSMEQAGFDRSTTLDVIQETANMAVHYVPSLRKAKIVRTFSGIRAMP
HHEECCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHCCCCC
KDGLPIIGRVPDVENLYVAATHSGITLSPLIGTIMTELIVDGETSIPIDRYSITRFS
CCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]