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The map label for this gene is sigL [H]

Identifier: 138896647

GI number: 138896647

Start: 3107125

End: 3108432

Strand: Reverse

Name: sigL [H]

Synonym: GTNG_3010

Alternate gene names: 138896647

Gene position: 3108432-3107125 (Counterclockwise)

Preceding gene: 138896649

Following gene: 138896646

Centisome position: 87.55

GC content: 55.66

Gene sequence:

>1308_bases
ATGAGGGCGGAGTTATGGCAAGAGCAGCGGCTGCAGTTGTCGCTGACGAAGGAGCTTGTGCAGGCGATTGAACTGCTGCA
ATATTCGGCACTTGATTTGGAAGCGTTTTTATACGAACAGTCGCTTGAAAACCCATTTTTAGAAATTCGCCGCGGTCGGA
TGCGGCGGAGAACAAGCCGAACGGACAAAGATCGGAAGCAATGGCTCGAAAATATCAGCGGTCGGACGGAGACGCTTGCT
GGCCACTTGCTTGCCCAACTTCCGGGATTGATGCTGCCGGCGAAGGAAGAGCGCATGGTTCGTCATCTGATCGCTGCGCT
GGATGAAGATGGCTATTTGCGCGTACCGCTTGCCGAGTTGGCAGCGCGATTCTCCGTATCGGAGCAAGAGGTCGAACGTG
CGCTGCGTTTTGTGCAATCGCTTGACCCACCGGGAGTCGGTGCGCGTGATTTGGCCGAGTGTCTCCTTCTGCAGCTTGAG
CGCCGGCCGGAGCGCGATGAGTTGGCGGAGACGATCGTTCGCCATCATTTTGTCGCCTTTGCCGAAAAAGCGTGGAAGAC
GCTTGCGAAACAACTCGGCGTCAGCCTCGGTGAGCTGCAGCGTGTCTTCGAGCTCATCCGCACGCTTGAGCCGCGGCCCG
GTATCCATTATGCGAACGAAACGCCGCATTTTATCGTGCCCGATTTGATCGTCACCCGCCAAGCGGACGGCGGTTGGATT
GTTACCTATAACGATGACATCCATCCGGAGCTCGGGTGGAATAAGGAATACGAGCGGCATATTGTTGCCGTTGGCGATCG
GCACGCTGAGCGATTTATGAAAGAAAAGTATCGGCAGTTTACATGGCTCGCCAAAAGTTTAGAACAACGGAAGCAGACGC
TAGTTCACCTCATGGGCATCATTATTGACCGCCAGCGCTCTTGTTTTGAAAGCGGCCTGTCGGCGCTGAAGCCGATGACG
ATGCGCGAAGTGGCCGAAGAACTCGGCGTCCATGAATCAACTGTGAGCCGTGCGGTCCGGCATAAATATGTGCAAACGCC
GTTTGGCATGATCGAGCTGCGTCGCTTTTTTTCCAGTGCTGCCACGGCTGGAGCGGGGGAGACGGCTTCCTCTGTTCAAG
TGAAAGCGATCATTCAAACGCTCATTTCCGCCGAAGACCGGCGGGCGCCGCTTTCCGACCAACAGCTTGCCGATTTGCTT
CGCGATCAGCATGGCATTGCCATCTCACGACGCACGGTCGCCAAATATCGCGAACAGCTGCGCATTCCGTCATCAGCCAA
ACGAAAACAATATACGAGCCTGCCGTAA

Upstream 100 bases:

>100_bases
AGTCCTCGGGTGCTTTTGTTTTGCCTGCGGGCGGCCGTCAAAAATTTCTCCTACCCCCCATCGTCACTGTTGTGTACAAT
AAAAGAAAGGGGGCGTGACG

Downstream 100 bases:

>100_bases
AGGCAATGAACAGAAAACAAGTGGCAAACGCTATAAGAAAGAAAGGAAGAAACACATGCACATCCGTCTGTATACAAAAA
CGAACTGTCCGCTTTGTGAA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MRAELWQEQRLQLSLTKELVQAIELLQYSALDLEAFLYEQSLENPFLEIRRGRMRRRTSRTDKDRKQWLENISGRTETLA
GHLLAQLPGLMLPAKEERMVRHLIAALDEDGYLRVPLAELAARFSVSEQEVERALRFVQSLDPPGVGARDLAECLLLQLE
RRPERDELAETIVRHHFVAFAEKAWKTLAKQLGVSLGELQRVFELIRTLEPRPGIHYANETPHFIVPDLIVTRQADGGWI
VTYNDDIHPELGWNKEYERHIVAVGDRHAERFMKEKYRQFTWLAKSLEQRKQTLVHLMGIIIDRQRSCFESGLSALKPMT
MREVAEELGVHESTVSRAVRHKYVQTPFGMIELRRFFSSAATAGAGETASSVQVKAIIQTLISAEDRRAPLSDQQLADLL
RDQHGIAISRRTVAKYREQLRIPSSAKRKQYTSLP

Sequences:

>Translated_435_residues
MRAELWQEQRLQLSLTKELVQAIELLQYSALDLEAFLYEQSLENPFLEIRRGRMRRRTSRTDKDRKQWLENISGRTETLA
GHLLAQLPGLMLPAKEERMVRHLIAALDEDGYLRVPLAELAARFSVSEQEVERALRFVQSLDPPGVGARDLAECLLLQLE
RRPERDELAETIVRHHFVAFAEKAWKTLAKQLGVSLGELQRVFELIRTLEPRPGIHYANETPHFIVPDLIVTRQADGGWI
VTYNDDIHPELGWNKEYERHIVAVGDRHAERFMKEKYRQFTWLAKSLEQRKQTLVHLMGIIIDRQRSCFESGLSALKPMT
MREVAEELGVHESTVSRAVRHKYVQTPFGMIELRRFFSSAATAGAGETASSVQVKAIIQTLISAEDRRAPLSDQQLADLL
RDQHGIAISRRTVAKYREQLRIPSSAKRKQYTSLP
>Mature_435_residues
MRAELWQEQRLQLSLTKELVQAIELLQYSALDLEAFLYEQSLENPFLEIRRGRMRRRTSRTDKDRKQWLENISGRTETLA
GHLLAQLPGLMLPAKEERMVRHLIAALDEDGYLRVPLAELAARFSVSEQEVERALRFVQSLDPPGVGARDLAECLLLQLE
RRPERDELAETIVRHHFVAFAEKAWKTLAKQLGVSLGELQRVFELIRTLEPRPGIHYANETPHFIVPDLIVTRQADGGWI
VTYNDDIHPELGWNKEYERHIVAVGDRHAERFMKEKYRQFTWLAKSLEQRKQTLVHLMGIIIDRQRSCFESGLSALKPMT
MREVAEELGVHESTVSRAVRHKYVQTPFGMIELRRFFSSAATAGAGETASSVQVKAIIQTLISAEDRRAPLSDQQLADLL
RDQHGIAISRRTVAKYREQLRIPSSAKRKQYTSLP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the levanase operon. The open complex (sigma-54 and core RNA poly

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=467, Percent_Identity=33.6188436830835, Blast_Score=235, Evalue=4e-63,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 50070; Mature: 50070

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAELWQEQRLQLSLTKELVQAIELLQYSALDLEAFLYEQSLENPFLEIRRGRMRRRTSR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
TDKDRKQWLENISGRTETLAGHLLAQLPGLMLPAKEERMVRHLIAALDEDGYLRVPLAEL
CCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEECCHHHH
AARFSVSEQEVERALRFVQSLDPPGVGARDLAECLLLQLERRPERDELAETIVRHHFVAF
HHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AEKAWKTLAKQLGVSLGELQRVFELIRTLEPRPGIHYANETPHFIVPDLIVTRQADGGWI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHEEEECCCCEE
VTYNDDIHPELGWNKEYERHIVAVGDRHAERFMKEKYRQFTWLAKSLEQRKQTLVHLMGI
EEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIDRQRSCFESGLSALKPMTMREVAEELGVHESTVSRAVRHKYVQTPFGMIELRRFFSSA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ATAGAGETASSVQVKAIIQTLISAEDRRAPLSDQQLADLLRDQHGIAISRRTVAKYREQL
HCCCCCCCHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RIPSSAKRKQYTSLP
CCCCHHHHHHCCCCC
>Mature Secondary Structure
MRAELWQEQRLQLSLTKELVQAIELLQYSALDLEAFLYEQSLENPFLEIRRGRMRRRTSR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
TDKDRKQWLENISGRTETLAGHLLAQLPGLMLPAKEERMVRHLIAALDEDGYLRVPLAEL
CCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEECCHHHH
AARFSVSEQEVERALRFVQSLDPPGVGARDLAECLLLQLERRPERDELAETIVRHHFVAF
HHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AEKAWKTLAKQLGVSLGELQRVFELIRTLEPRPGIHYANETPHFIVPDLIVTRQADGGWI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHEEEECCCCEE
VTYNDDIHPELGWNKEYERHIVAVGDRHAERFMKEKYRQFTWLAKSLEQRKQTLVHLMGI
EEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIDRQRSCFESGLSALKPMTMREVAEELGVHESTVSRAVRHKYVQTPFGMIELRRFFSSA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ATAGAGETASSVQVKAIIQTLISAEDRRAPLSDQQLADLLRDQHGIAISRRTVAKYREQL
HCCCCCCCHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RIPSSAKRKQYTSLP
CCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1924373; 8969506; 9384377 [H]