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The map label for this gene is eno [H]

Identifier: 138896640

GI number: 138896640

Start: 3099648

End: 3100940

Strand: Reverse

Name: eno [H]

Synonym: GTNG_3003

Alternate gene names: 138896640

Gene position: 3100940-3099648 (Counterclockwise)

Preceding gene: 138896641

Following gene: 138896639

Centisome position: 87.34

GC content: 50.89

Gene sequence:

>1293_bases
ATGTCTGCGATTATTGATGTGTATGCGCGTGAAGTGCTCGATTCGCGCGGCAATCCAACCGTAGAAGTGGAAGTATACAC
GGAAGAAGGCGGCTTCGGCCGTGCGTTAGTGCCAAGCGGCGCTTCGACGGGCGAATATGAAGCGGTTGAATTGCGTGACG
GCGACAAAAACCGCTACCTCGGCAAAGGGGTGCTCAAAGCGGTTGAGAACGTCAACGAAGTGATTGCTCCGGAAATCATC
GGCTTAGAAGTGACTGATCAAGTGGCGATCGACCGCGCGTTGATTGAACTTGACGGCACGGAAAACAAAGGAAAGCTTGG
GGCGAATGCTATTTTAGGCGTGTCGCTCGCGGTCGCTCGCGCTGCGGCTGATGAGCTTGGCTTGCCGTTGTACCAATACT
TGGGCGGCTTTAACGCTAAAACGCTGCCTGTACCGATGATGAACATTTTAAACGGCGGCGCGCATGCGGACAACAACGTT
GACATTCAAGAATTCATGATCATGCCGGTCGGTGCGGAAAGCTTCCGTGAAGCGCTGCGCATGGGTGCAGAAATTTTCCA
TAGCTTAAAAGCTGTGTTAAAAGCGAAAGGCTACAACACGGCTGTCGGTGACGAAGGCGGATTTGCTCCGAACTTAAAAT
CGAACGAAGAAGCGCTGCAAACGATCATTGAAGCGATCGAAAAAGCCGGCTACAAACCAGGCGAACAAGTGATGCTCGCT
ATGGACGTTGCTTCGTCGGAGCTGTACAACAAAGAAGATGGCAAATATCATTTGGAAGGCGAAGGCGTCGTCAAAACATC
AGAAGAAATGGTTGCTTGGTATGAAGAGCTTGTGTCGAAATATCCGATCATCTCGATCGAAGACGGACTTGACGAAAATG
ACTGGGAAGGCCATAAACTGCTTACTGAGCGCCTTGGCCACAAAGTGCAGCTCGTCGGTGACGACTTGTTTGTAACGAAC
ACGAAAAAACTGGCCGAAGGCATTGAAAAAGGCGTCGGCAACTCGATTTTAATTAAAGTGAACCAAATCGGTACACTGAC
GGAAACGTTCGATGCCATTGAGATGGCCAAACGCGCCGGCTACACGGCGGTTGTGTCGCACCGTTCCGGTGAAACGGAAG
ACAGCACGATTGCCGATATCGCTGTCGCAACAAACGCTGGCCAAATCAAAACGGGAGCACCGTCGCGTACGGACCGCGTC
GCAAAATACAACCAGCTGCTCCGCATTGAAGACGAACTTGGCCACACGGCTATTTACCAAGGCATTCGTTCGTTTTACAA
TTTGAAAAAATAA

Upstream 100 bases:

>100_bases
CGCCGACGATGCTTGATTTGCTCGGCTTGCCGCAACCGAAAGAAATGACCGGAAAAACGTTAATCGTTAAATAACAAACA
CGGAAAAGGAGAGTGTCGAT

Downstream 100 bases:

>100_bases
CGGGAATCAACAACAAAGGGTGTCTCCAACGTTGCGAGACACCCTCTTTAATTACGGGAAACAGAAATGATTTCCTATCG
ATAGCAAAAAATGGACGTGG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSAIIDVYAREVLDSRGNPTVEVEVYTEEGGFGRALVPSGASTGEYEAVELRDGDKNRYLGKGVLKAVENVNEVIAPEII
GLEVTDQVAIDRALIELDGTENKGKLGANAILGVSLAVARAAADELGLPLYQYLGGFNAKTLPVPMMNILNGGAHADNNV
DIQEFMIMPVGAESFREALRMGAEIFHSLKAVLKAKGYNTAVGDEGGFAPNLKSNEEALQTIIEAIEKAGYKPGEQVMLA
MDVASSELYNKEDGKYHLEGEGVVKTSEEMVAWYEELVSKYPIISIEDGLDENDWEGHKLLTERLGHKVQLVGDDLFVTN
TKKLAEGIEKGVGNSILIKVNQIGTLTETFDAIEMAKRAGYTAVVSHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRV
AKYNQLLRIEDELGHTAIYQGIRSFYNLKK

Sequences:

>Translated_430_residues
MSAIIDVYAREVLDSRGNPTVEVEVYTEEGGFGRALVPSGASTGEYEAVELRDGDKNRYLGKGVLKAVENVNEVIAPEII
GLEVTDQVAIDRALIELDGTENKGKLGANAILGVSLAVARAAADELGLPLYQYLGGFNAKTLPVPMMNILNGGAHADNNV
DIQEFMIMPVGAESFREALRMGAEIFHSLKAVLKAKGYNTAVGDEGGFAPNLKSNEEALQTIIEAIEKAGYKPGEQVMLA
MDVASSELYNKEDGKYHLEGEGVVKTSEEMVAWYEELVSKYPIISIEDGLDENDWEGHKLLTERLGHKVQLVGDDLFVTN
TKKLAEGIEKGVGNSILIKVNQIGTLTETFDAIEMAKRAGYTAVVSHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRV
AKYNQLLRIEDELGHTAIYQGIRSFYNLKK
>Mature_429_residues
SAIIDVYAREVLDSRGNPTVEVEVYTEEGGFGRALVPSGASTGEYEAVELRDGDKNRYLGKGVLKAVENVNEVIAPEIIG
LEVTDQVAIDRALIELDGTENKGKLGANAILGVSLAVARAAADELGLPLYQYLGGFNAKTLPVPMMNILNGGAHADNNVD
IQEFMIMPVGAESFREALRMGAEIFHSLKAVLKAKGYNTAVGDEGGFAPNLKSNEEALQTIIEAIEKAGYKPGEQVMLAM
DVASSELYNKEDGKYHLEGEGVVKTSEEMVAWYEELVSKYPIISIEDGLDENDWEGHKLLTERLGHKVQLVGDDLFVTNT
KKLAEGIEKGVGNSILIKVNQIGTLTETFDAIEMAKRAGYTAVVSHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRVA
KYNQLLRIEDELGHTAIYQGIRSFYNLKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=437, Percent_Identity=54.9199084668192, Blast_Score=458, Evalue=1e-129,
Organism=Homo sapiens, GI301897477, Length=439, Percent_Identity=54.2141230068337, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI301897469, Length=439, Percent_Identity=54.2141230068337, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI4503571, Length=437, Percent_Identity=52.8604118993135, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI301897479, Length=437, Percent_Identity=48.9702517162471, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.4880952380952, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.4880952380952, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.4880952380952, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=65.2582159624413, Blast_Score=536, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=53.5469107551487, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17536383, Length=437, Percent_Identity=53.5469107551487, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI32563855, Length=198, Percent_Identity=45.4545454545455, Blast_Score=178, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=52.3041474654378, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324974, Length=436, Percent_Identity=50.6880733944954, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324969, Length=436, Percent_Identity=50.6880733944954, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6323985, Length=436, Percent_Identity=50.4587155963303, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=51.8433179723502, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=53.9351851851852, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=53.9351851851852, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=53.9351851851852, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=53.9351851851852, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=53.9351851851852, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=53.9351851851852, Blast_Score=416, Evalue=1e-116,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46556; Mature: 46425

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIIDVYAREVLDSRGNPTVEVEVYTEEGGFGRALVPSGASTGEYEAVELRDGDKNRYL
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCCCCHH
GKGVLKAVENVNEVIAPEIIGLEVTDQVAIDRALIELDGTENKGKLGANAILGVSLAVAR
HHHHHHHHHHHHHHHCCHHCCEEECHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADELGLPLYQYLGGFNAKTLPVPMMNILNGGAHADNNVDIQEFMIMPVGAESFREALR
HHHHHHCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEECCCHHHHHHHHH
MGAEIFHSLKAVLKAKGYNTAVGDEGGFAPNLKSNEEALQTIIEAIEKAGYKPGEQVMLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEEE
MDVASSELYNKEDGKYHLEGEGVVKTSEEMVAWYEELVSKYPIISIEDGLDENDWEGHKL
EEHHHHHHCCCCCCEEEECCCCEEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTERLGHKVQLVGDDLFVTNTKKLAEGIEKGVGNSILIKVNQIGTLTETFDAIEMAKRAG
HHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVVSHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEDELGHTAIYQ
CEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCHHHHHHHHHHHEEEHHHHCHHHHHH
GIRSFYNLKK
HHHHHHCCCC
>Mature Secondary Structure 
SAIIDVYAREVLDSRGNPTVEVEVYTEEGGFGRALVPSGASTGEYEAVELRDGDKNRYL
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCCCCHH
GKGVLKAVENVNEVIAPEIIGLEVTDQVAIDRALIELDGTENKGKLGANAILGVSLAVAR
HHHHHHHHHHHHHHHCCHHCCEEECHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADELGLPLYQYLGGFNAKTLPVPMMNILNGGAHADNNVDIQEFMIMPVGAESFREALR
HHHHHHCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEECCCHHHHHHHHH
MGAEIFHSLKAVLKAKGYNTAVGDEGGFAPNLKSNEEALQTIIEAIEKAGYKPGEQVMLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEEE
MDVASSELYNKEDGKYHLEGEGVVKTSEEMVAWYEELVSKYPIISIEDGLDENDWEGHKL
EEHHHHHHCCCCCCEEEECCCCEEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTERLGHKVQLVGDDLFVTNTKKLAEGIEKGVGNSILIKVNQIGTLTETFDAIEMAKRAG
HHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVVSHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEDELGHTAIYQ
CEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCHHHHHHHHHHHEEEHHHHCHHHHHH
GIRSFYNLKK
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA