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The map label for this gene is hpaG [H]

Identifier: 138896625

GI number: 138896625

Start: 3084242

End: 3084979

Strand: Reverse

Name: hpaG [H]

Synonym: GTNG_2988

Alternate gene names: 138896625

Gene position: 3084979-3084242 (Counterclockwise)

Preceding gene: 138896626

Following gene: 138896624

Centisome position: 86.89

GC content: 55.96

Gene sequence:

>738_bases
ATGAAGCAGGCGCGCGTCGCGTGGAACGGAACGGTGACCGAAGCCGAGCCGCTTGACAGCGGCCGCCTTCTTCTGCCGGA
TGGAACGATCGTCGATGAGCATGAGGTGATGTGGCTTCCCCCGGTCGAGCCGCGCACGATCGTGGCGCTTGGGTTGAATT
ACGCGGATCATGCCGGGGAGTTGTCGTTTGCCGCTCCGAAAGAGCCGCTTTTATTTTTCAAAGGGAAAAACACGCTAATC
GGCCACCGCGGCCGCACCGTCCGGCCGGCAGGGGTTGACATGATGCATTACGAATGCGAACTCGCCGTGGTCATCGGCCG
CCCGGCTCGTCGAGTGAAGGCAGAACAGGCGTATGATTACATTTGGGGGTACACGATTGCCAACGATTACGCTGTTCGCG
ATTACTTGGAAAATTACTACCGACCGAATTTTCGCGTTAAAAACCGTGACCGAACAACGCCGCTCGGCCCGTGGATTGTC
AGCCATGAGGAGGTTGGCGATCCGATGGCGCTGGCGTTGCGGACGTATGTTAATGGAGCGCTCGTGCAGGAAGGATATAC
AGGCGATATGATTTTTTCCATTCCGTATTTGCTTGAATATATTACGAGTTTTATGACGCTCGCGCCAGGCGATCTGATTT
TGACCGGCACGCCGAAAGGGGCGGTGAACGTTCAGCCCGGCGATGAAGTCGTCGCGGAAATTGAGCGTATCGGCCGGCTC
ATCAATTACATTGAATAA

Upstream 100 bases:

>100_bases
ACGCCCCGCTCGTTTCGGCAGGGGATGTGGTGCGCATCGAAATGGACGGGATCGGCGCGTTGGAAAATCCGATCGTTGCC
GAAGAAGGAGGAGAAGGGGA

Downstream 100 bases:

>100_bases
CCAGCATCCAACGATGCGCGAACCATTCAGTTAAAGAGCACGGGGCCTTTTCAGAACGAAAGGGAGGAAGCGCGATGCCG
CACTTTGTTTTGGAATATAC

Product: putative 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKQARVAWNGTVTEAEPLDSGRLLLPDGTIVDEHEVMWLPPVEPRTIVALGLNYADHAGELSFAAPKEPLLFFKGKNTLI
GHRGRTVRPAGVDMMHYECELAVVIGRPARRVKAEQAYDYIWGYTIANDYAVRDYLENYYRPNFRVKNRDRTTPLGPWIV
SHEEVGDPMALALRTYVNGALVQEGYTGDMIFSIPYLLEYITSFMTLAPGDLILTGTPKGAVNVQPGDEVVAEIERIGRL
INYIE

Sequences:

>Translated_245_residues
MKQARVAWNGTVTEAEPLDSGRLLLPDGTIVDEHEVMWLPPVEPRTIVALGLNYADHAGELSFAAPKEPLLFFKGKNTLI
GHRGRTVRPAGVDMMHYECELAVVIGRPARRVKAEQAYDYIWGYTIANDYAVRDYLENYYRPNFRVKNRDRTTPLGPWIV
SHEEVGDPMALALRTYVNGALVQEGYTGDMIFSIPYLLEYITSFMTLAPGDLILTGTPKGAVNVQPGDEVVAEIERIGRL
INYIE
>Mature_245_residues
MKQARVAWNGTVTEAEPLDSGRLLLPDGTIVDEHEVMWLPPVEPRTIVALGLNYADHAGELSFAAPKEPLLFFKGKNTLI
GHRGRTVRPAGVDMMHYECELAVVIGRPARRVKAEQAYDYIWGYTIANDYAVRDYLENYYRPNFRVKNRDRTTPLGPWIV
SHEEVGDPMALALRTYVNGALVQEGYTGDMIFSIPYLLEYITSFMTLAPGDLILTGTPKGAVNVQPGDEVVAEIERIGRL
INYIE

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=232, Percent_Identity=36.6379310344828, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI40786394, Length=219, Percent_Identity=37.8995433789954, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI66348062, Length=193, Percent_Identity=39.3782383419689, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI13654274, Length=204, Percent_Identity=37.7450980392157, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI215422413, Length=193, Percent_Identity=39.3782383419689, Blast_Score=131, Evalue=7e-31,
Organism=Escherichia coli, GI1787428, Length=192, Percent_Identity=37.5, Blast_Score=127, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17557057, Length=187, Percent_Identity=41.1764705882353, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324161, Length=225, Percent_Identity=29.3333333333333, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28572127, Length=219, Percent_Identity=40.1826484018265, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24663695, Length=224, Percent_Identity=31.25, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI28571789, Length=204, Percent_Identity=35.7843137254902, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 27469; Mature: 27469

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQARVAWNGTVTEAEPLDSGRLLLPDGTIVDEHEVMWLPPVEPRTIVALGLNYADHAGE
CCCCEEEECCCCCCCCCCCCCCEECCCCCEECCCCEEEECCCCCCEEEEEECCCCCCCCC
LSFAAPKEPLLFFKGKNTLIGHRGRTVRPAGVDMMHYECELAVVIGRPARRVKAEQAYDY
EEECCCCCCEEEEECCCCEEECCCCEECCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHH
IWGYTIANDYAVRDYLENYYRPNFRVKNRDRTTPLGPWIVSHEEVGDPMALALRTYVNGA
HEEEEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECHHHCCCHHHHHHHHHHCCE
LVQEGYTGDMIFSIPYLLEYITSFMTLAPGDLILTGTPKGAVNVQPGDEVVAEIERIGRL
EEECCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHH
INYIE
HHHCC
>Mature Secondary Structure
MKQARVAWNGTVTEAEPLDSGRLLLPDGTIVDEHEVMWLPPVEPRTIVALGLNYADHAGE
CCCCEEEECCCCCCCCCCCCCCEECCCCCEECCCCEEEECCCCCCEEEEEECCCCCCCCC
LSFAAPKEPLLFFKGKNTLIGHRGRTVRPAGVDMMHYECELAVVIGRPARRVKAEQAYDY
EEECCCCCCEEEEECCCCEEECCCCEECCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHH
IWGYTIANDYAVRDYLENYYRPNFRVKNRDRTTPLGPWIVSHEEVGDPMALALRTYVNGA
HEEEEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECHHHCCCHHHHHHHHHHCCE
LVQEGYTGDMIFSIPYLLEYITSFMTLAPGDLILTGTPKGAVNVQPGDEVVAEIERIGRL
EEECCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHH
INYIE
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA