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The map label for this gene is mcbR [H]

Identifier: 138896622

GI number: 138896622

Start: 3081561

End: 3082226

Strand: Reverse

Name: mcbR [H]

Synonym: GTNG_2985

Alternate gene names: 138896622

Gene position: 3082226-3081561 (Counterclockwise)

Preceding gene: 138896623

Following gene: 138896621

Centisome position: 86.82

GC content: 52.55

Gene sequence:

>666_bases
ATGGTATATGATAAACGTATGGAAGCAAAAGCGAAAAACAAGACACAGTTGGCGTATGAGTATATTTTGTCCCACATCGA
AAGCGGCGCCTACGGGCCAGGATATCGTGTCGTCATCGATCAAATTGCTCGCGAGTTGGGGTTAAGCAGCATCCCGGTGC
GCGAGGCGATCCGCCAGCTCGAGGCGGAAGGATTGGTCGAGTTTAAGCCGTATGCTGGCGCCGTTGTCAGCACGATTAAC
GAGAAAGAATACGTGGAAACCTTGTCTGTGTTGGCGGTTTTAGAGGGCTATGCGACGGCGCTCGGCTCCGCTAAACTAAC
GAAGGAGGCGATCAAGCAGCTGGAACAGTTGAACGAGCAGATGGAGCGAGCGCTGGAAGAGCTGGAGCTGGAACGGTTCA
GCGAGCTGAACTATGCGTTCCATTCTTTGATTTATTCGCACTGTGGCAATGCGTATTTAGAAGAGCAAATCAAGCAAATT
TGGCAGCGCATGAAGCGCATTCGCGTTTACGGGTTCACCTTCGTCCCGCAGCGGGCGAAAGCTTCGATTGGAGAACATCG
GGAAATGATCCGCCTGTTGCGCGAACAAGCACCGCCGCACGAGGTGGAGCAGTATGCGCGCCAACATAAAATGAACACCA
TCGAAGCATTTTTGCGGCGGCGTTAA

Upstream 100 bases:

>100_bases
TGTCGCCGTCGGCGACCCGCCGATTCCTTCATTCGGCAAAACGAAGCCTTCATCCGCCTTATCGGTCGAACAGAAATAAC
TGCTAGCGGACATATATGGC

Downstream 100 bases:

>100_bases
CTTCCTTCCGCCAAGCAGGAGCGCTTGGCGGAGGAAGGCTGATTTGATTGGAAGCGATTTCAATTAAAAGGGGGGATAAA
GACGATGAGAAAAAGGGTGT

Product: putative transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MVYDKRMEAKAKNKTQLAYEYILSHIESGAYGPGYRVVIDQIARELGLSSIPVREAIRQLEAEGLVEFKPYAGAVVSTIN
EKEYVETLSVLAVLEGYATALGSAKLTKEAIKQLEQLNEQMERALEELELERFSELNYAFHSLIYSHCGNAYLEEQIKQI
WQRMKRIRVYGFTFVPQRAKASIGEHREMIRLLREQAPPHEVEQYARQHKMNTIEAFLRRR

Sequences:

>Translated_221_residues
MVYDKRMEAKAKNKTQLAYEYILSHIESGAYGPGYRVVIDQIARELGLSSIPVREAIRQLEAEGLVEFKPYAGAVVSTIN
EKEYVETLSVLAVLEGYATALGSAKLTKEAIKQLEQLNEQMERALEELELERFSELNYAFHSLIYSHCGNAYLEEQIKQI
WQRMKRIRVYGFTFVPQRAKASIGEHREMIRLLREQAPPHEVEQYARQHKMNTIEAFLRRR
>Mature_221_residues
MVYDKRMEAKAKNKTQLAYEYILSHIESGAYGPGYRVVIDQIARELGLSSIPVREAIRQLEAEGLVEFKPYAGAVVSTIN
EKEYVETLSVLAVLEGYATALGSAKLTKEAIKQLEQLNEQMERALEELELERFSELNYAFHSLIYSHCGNAYLEEQIKQI
WQRMKRIRVYGFTFVPQRAKASIGEHREMIRLLREQAPPHEVEQYARQHKMNTIEAFLRRR

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=149, Percent_Identity=30.8724832214765, Blast_Score=70, Evalue=8e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25640; Mature: 25640

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYDKRMEAKAKNKTQLAYEYILSHIESGAYGPGYRVVIDQIARELGLSSIPVREAIRQL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
EAEGLVEFKPYAGAVVSTINEKEYVETLSVLAVLEGYATALGSAKLTKEAIKQLEQLNEQ
HHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MERALEELELERFSELNYAFHSLIYSHCGNAYLEEQIKQIWQRMKRIRVYGFTFVPQRAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ASIGEHREMIRLLREQAPPHEVEQYARQHKMNTIEAFLRRR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVYDKRMEAKAKNKTQLAYEYILSHIESGAYGPGYRVVIDQIARELGLSSIPVREAIRQL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
EAEGLVEFKPYAGAVVSTINEKEYVETLSVLAVLEGYATALGSAKLTKEAIKQLEQLNEQ
HHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MERALEELELERFSELNYAFHSLIYSHCGNAYLEEQIKQIWQRMKRIRVYGFTFVPQRAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ASIGEHREMIRLLREQAPPHEVEQYARQHKMNTIEAFLRRR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]