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The map label for this gene is ntaB [H]

Identifier: 138896616

GI number: 138896616

Start: 3076170

End: 3076637

Strand: Reverse

Name: ntaB [H]

Synonym: GTNG_2979

Alternate gene names: 138896616

Gene position: 3076637-3076170 (Counterclockwise)

Preceding gene: 138896617

Following gene: 138896615

Centisome position: 86.66

GC content: 51.71

Gene sequence:

>468_bases
ATGGATGATCGCACATTTCGCAACGCGATGGGGAAATTCGCAACCGGTGTGACGGTCGTGACGACCGAATTTCAAGGGGA
AGTGAAAGGGATGACGGCGAACGCGTTTATGTCCGTTTCGCTTGATCCGAAGCTGGTGGTCGTCTCGATTGGCCATAAAG
CGCGGATGCATGACATCGTCAAGCAAACCGGGAAGTTTGCGGTCAATCTTTTGACACGCGACCAAGAAGACTTGTCGCGT
TTGTTTGCGGGTCAGCTGAAGGAAGAACGGGATGTGGCGTTTGAATGGGTGGACGGCCACCCGATTTTGCCGGATGCGAT
GGCGAATGTGTTATGCCGTGTGCACAGCACGTACGTCGCCGGCGACCATACGCTTTATTTCGGCGAAGTGACCCATATTG
TGATGAAAGATGAACCAGGCGATCCGCTCTTATTTTTTGAAGGAAAATATCGAAGCATTGGCCAATAA

Upstream 100 bases:

>100_bases
TTGCCGTATAACATCGAAATGATGGGACGGCCATCGCTTTAGGCTCTGTCCTTTAGCTGTGCAAACGAAGTTTACGAAAA
GGGAAAGGATGGGAGAACTG

Downstream 100 bases:

>100_bases
GGATGAGGCAGGTGTGCTGGCCGGCGGATAGCCGGCTAGGCATCTGCTGTTTCGTGAAGAAGTTGAGAGAGGTTTACGCA
CGAAAGAGTTTCTCAGTCAT

Product: flavin reductase

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MDDRTFRNAMGKFATGVTVVTTEFQGEVKGMTANAFMSVSLDPKLVVVSIGHKARMHDIVKQTGKFAVNLLTRDQEDLSR
LFAGQLKEERDVAFEWVDGHPILPDAMANVLCRVHSTYVAGDHTLYFGEVTHIVMKDEPGDPLLFFEGKYRSIGQ

Sequences:

>Translated_155_residues
MDDRTFRNAMGKFATGVTVVTTEFQGEVKGMTANAFMSVSLDPKLVVVSIGHKARMHDIVKQTGKFAVNLLTRDQEDLSR
LFAGQLKEERDVAFEWVDGHPILPDAMANVLCRVHSTYVAGDHTLYFGEVTHIVMKDEPGDPLLFFEGKYRSIGQ
>Mature_155_residues
MDDRTFRNAMGKFATGVTVVTTEFQGEVKGMTANAFMSVSLDPKLVVVSIGHKARMHDIVKQTGKFAVNLLTRDQEDLSR
LFAGQLKEERDVAFEWVDGHPILPDAMANVLCRVHSTYVAGDHTLYFGEVTHIVMKDEPGDPLLFFEGKYRSIGQ

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=157, Percent_Identity=29.9363057324841, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 17306; Mature: 17306

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDRTFRNAMGKFATGVTVVTTEFQGEVKGMTANAFMSVSLDPKLVVVSIGHKARMHDIV
CCCHHHHHHHHHHHCCEEEEEEECCCEECCEEECEEEEEECCCEEEEEECCCHHHHHHHH
KQTGKFAVNLLTRDQEDLSRLFAGQLKEERDVAFEWVDGHPILPDAMANVLCRVHSTYVA
HHHHHEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEEEC
GDHTLYFGEVTHIVMKDEPGDPLLFFEGKYRSIGQ
CCCEEEEEEEEEEEEECCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MDDRTFRNAMGKFATGVTVVTTEFQGEVKGMTANAFMSVSLDPKLVVVSIGHKARMHDIV
CCCHHHHHHHHHHHCCEEEEEEECCCEECCEEECEEEEEECCCEEEEEECCCHHHHHHHH
KQTGKFAVNLLTRDQEDLSRLFAGQLKEERDVAFEWVDGHPILPDAMANVLCRVHSTYVA
HHHHHEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEEEC
GDHTLYFGEVTHIVMKDEPGDPLLFFEGKYRSIGQ
CCCEEEEEEEEEEEEECCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]