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The map label for this gene is lipA [H]

Identifier: 138896561

GI number: 138896561

Start: 3026708

End: 3027604

Strand: Reverse

Name: lipA [H]

Synonym: GTNG_2924

Alternate gene names: 138896561

Gene position: 3027604-3026708 (Counterclockwise)

Preceding gene: 138896563

Following gene: 138896559

Centisome position: 85.28

GC content: 51.39

Gene sequence:

>897_bases
ATGGCGACGAAGGAAGAACATGTCCGCAAGCCAGATTGGTTAAAAATCAAGCTGAATACGAACGAACATTATACCGGATT
GAAGAAGTTGATGCGGGAAAACCGTTTACATACTGTATGCGAGGAAGCGAAATGCCCGAACATTCATGAATGTTGGGCAG
TACGGCGGACCGCGACGTTTATGATTTTAGGCAGCGTCTGTACGCGCGCCTGCCGCTTTTGCGCCGTTAAAACGGGGCTG
CCGACCGAGCTGGACTGGCAAGAACCGGAACGCGTCGCCGAATCCGTGCGCATCATGAACTTAAAACATGTCGTTGTCAC
CGCTGTGGCTCGCGATGATTTGAAAGATGGGGGAGCGGCGGTATTTGCGGAAACGGTGCGTGCCATCCGCCGGAAAAACC
CGTTTACGACGATTGAAGTGTTGCCGTCTGATATGGGTGGGGTGTACGAAAACTTAAAAACGTTAATGGACGCCCGTCCT
GATATTTTAAACCATAACATCGAAACGGTGCGCCGCCTGACGCCGCGGGTGCGCGCCCGCGCGACGTACGAGCGTTCGCT
TGAATTTTTGCGGCGCGCGAAAGAACTGCAGCCGGATATTCCGACGAAATCAAGCATCATGATCGGTCTTGGGGAAACGA
AAGAGGAAATTATTGAGGCGATGGACGATTTGCGCGCCAACCATGTCGATATTTTGACGATCGGTCAATATTTGCAGCCG
ACGAAAAAACATTTGAAAGTCGTCAAATATTACCATCCTGATGAATTCCAAGAGCTAAAAGAAATCGCGTTAAGCAAAGG
ATTCAGCCATTGCGAGGCCGGCCCGCTCGTTCGTTCATCGTACCATGCTGACGAGCAGGTGAACGAAGCAGCAAAGGCGC
GGCAACTGAAGGCATAA

Upstream 100 bases:

>100_bases
TTAAAAAGTAGAAAAGGGAAAAACGTCTTATTTTTTGTGTTTTCCCGCGCTTTTCATTATAATGAGAACTGAACAATCAT
GCATAACTGGAGTGAGAACA

Downstream 100 bases:

>100_bases
TAGCAGCTACGCAAATGAAAAACGGTCGTGCCTCTTCGTCAAGAAGGCAGACCGTTTTCTTGCCCTAGCGTCCTTTCGGC
GTCGGCCGCGGTGTGCCGTC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MATKEEHVRKPDWLKIKLNTNEHYTGLKKLMRENRLHTVCEEAKCPNIHECWAVRRTATFMILGSVCTRACRFCAVKTGL
PTELDWQEPERVAESVRIMNLKHVVVTAVARDDLKDGGAAVFAETVRAIRRKNPFTTIEVLPSDMGGVYENLKTLMDARP
DILNHNIETVRRLTPRVRARATYERSLEFLRRAKELQPDIPTKSSIMIGLGETKEEIIEAMDDLRANHVDILTIGQYLQP
TKKHLKVVKYYHPDEFQELKEIALSKGFSHCEAGPLVRSSYHADEQVNEAAKARQLKA

Sequences:

>Translated_298_residues
MATKEEHVRKPDWLKIKLNTNEHYTGLKKLMRENRLHTVCEEAKCPNIHECWAVRRTATFMILGSVCTRACRFCAVKTGL
PTELDWQEPERVAESVRIMNLKHVVVTAVARDDLKDGGAAVFAETVRAIRRKNPFTTIEVLPSDMGGVYENLKTLMDARP
DILNHNIETVRRLTPRVRARATYERSLEFLRRAKELQPDIPTKSSIMIGLGETKEEIIEAMDDLRANHVDILTIGQYLQP
TKKHLKVVKYYHPDEFQELKEIALSKGFSHCEAGPLVRSSYHADEQVNEAAKARQLKA
>Mature_297_residues
ATKEEHVRKPDWLKIKLNTNEHYTGLKKLMRENRLHTVCEEAKCPNIHECWAVRRTATFMILGSVCTRACRFCAVKTGLP
TELDWQEPERVAESVRIMNLKHVVVTAVARDDLKDGGAAVFAETVRAIRRKNPFTTIEVLPSDMGGVYENLKTLMDARPD
ILNHNIETVRRLTPRVRARATYERSLEFLRRAKELQPDIPTKSSIMIGLGETKEEIIEAMDDLRANHVDILTIGQYLQPT
KKHLKVVKYYHPDEFQELKEIALSKGFSHCEAGPLVRSSYHADEQVNEAAKARQLKA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=288, Percent_Identity=44.4444444444444, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=45.2, Blast_Score=223, Evalue=2e-58,
Organism=Escherichia coli, GI1786846, Length=285, Percent_Identity=52.280701754386, Blast_Score=295, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI32564533, Length=290, Percent_Identity=45.1724137931035, Blast_Score=239, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6324770, Length=300, Percent_Identity=48.3333333333333, Blast_Score=276, Evalue=3e-75,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=47.9166666666667, Blast_Score=266, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34097; Mature: 33966

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKEEHVRKPDWLKIKLNTNEHYTGLKKLMRENRLHTVCEEAKCPNIHECWAVRRTATF
CCCCHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MILGSVCTRACRFCAVKTGLPTELDWQEPERVAESVRIMNLKHVVVTAVARDDLKDGGAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VFAETVRAIRRKNPFTTIEVLPSDMGGVYENLKTLMDARPDILNHNIETVRRLTPRVRAR
HHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHH
ATYERSLEFLRRAKELQPDIPTKSSIMIGLGETKEEIIEAMDDLRANHVDILTIGQYLQP
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEHHHHHCH
TKKHLKVVKYYHPDEFQELKEIALSKGFSHCEAGPLVRSSYHADEQVNEAAKARQLKA
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATKEEHVRKPDWLKIKLNTNEHYTGLKKLMRENRLHTVCEEAKCPNIHECWAVRRTATF
CCCHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MILGSVCTRACRFCAVKTGLPTELDWQEPERVAESVRIMNLKHVVVTAVARDDLKDGGAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VFAETVRAIRRKNPFTTIEVLPSDMGGVYENLKTLMDARPDILNHNIETVRRLTPRVRAR
HHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHH
ATYERSLEFLRRAKELQPDIPTKSSIMIGLGETKEEIIEAMDDLRANHVDILTIGQYLQP
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEHHHHHCH
TKKHLKVVKYYHPDEFQELKEIALSKGFSHCEAGPLVRSSYHADEQVNEAAKARQLKA
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA