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The map label for this gene is nadE [H]

Identifier: 138896163

GI number: 138896163

Start: 2630492

End: 2631232

Strand: Reverse

Name: nadE [H]

Synonym: GTNG_2526

Alternate gene names: 138896163

Gene position: 2631232-2630492 (Counterclockwise)

Preceding gene: 138896164

Following gene: 138896162

Centisome position: 74.11

GC content: 51.01

Gene sequence:

>741_bases
ATGCAAGAAAAAATTGACAAGCTTGTCCAATGGCTGCGGGAACAAGTGGCGTCGGCTGGCTTAAACGGGGCAGTAGTCGG
CATTAGCGGTGGCATCGACTCTGCCGTTGTTGCCCATTTGATCAAGCGGGCGTTTCCAGATAACTCGCTTGGCCTCATCA
TGCCGTGCAAAAGTCATGCAAAAGATATGGAAGATGCCCTCAAAGTGGTGGAAAGCTGCGGCATTCAACATCTTGTCATT
GATTTGACCGAGGTGCATAAATCGTTGTTCGGCGCGGTGGAAGCCGAGTTGAAAGCCATCGGTGAGTGGAATGAAGAAAA
GGCGCGGCTTGGCGATGCGAACACGAGGGCGCGTCTGCGCATGACGACGTTGTACGCTGTCGCCAACAACTATGGCTACC
TCGTTGTCGGTACGGACAATGCGGCGGAGTGGCACACCGGCTACTTTACGAAATATGGCGATGGCGGGGTTGATTTAGTG
CCGCTCATTCATTTCACAAAAGGCGAAGTGCGTGAAATGGCTCGTCTGCTTGGTGTTCCGGAAGAAATTATCACGAAAGC
GCCGAGCGCCGGATTATGGGAAGGACAGACGGATGAAAGCGAAATGGGCACGACGTATGATATGATTGATAAATATTTAA
AAGGTGAAGACATTCCAGAGCAAGACCGGAAAATTATTGAACGGCTGCATGAACGTTCGCACCATAAACGGCAGCTGGCC
ATTGCGCCACCAAAGTTTTAA

Upstream 100 bases:

>100_bases
CGCTCGTATTGGATCGGAAGAAGCGGGAATGGTTTGTCCGTCGTCTTTTCGCTATGCTAAAATAAAGAGTAAAATCTTTG
GAAAAAGGAGTCGAAGGCGG

Downstream 100 bases:

>100_bases
CCGTCGTCTTGTTGGTTTGAACTAACAGCGATAACCCCCCTGCTTTTTCCGCAAGCTAAATGTAAGGCTGGCTTTGCGCC
AGCCTTGGGCGAAGGAAACC

Product: NH(3)-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MQEKIDKLVQWLREQVASAGLNGAVVGISGGIDSAVVAHLIKRAFPDNSLGLIMPCKSHAKDMEDALKVVESCGIQHLVI
DLTEVHKSLFGAVEAELKAIGEWNEEKARLGDANTRARLRMTTLYAVANNYGYLVVGTDNAAEWHTGYFTKYGDGGVDLV
PLIHFTKGEVREMARLLGVPEEIITKAPSAGLWEGQTDESEMGTTYDMIDKYLKGEDIPEQDRKIIERLHERSHHKRQLA
IAPPKF

Sequences:

>Translated_246_residues
MQEKIDKLVQWLREQVASAGLNGAVVGISGGIDSAVVAHLIKRAFPDNSLGLIMPCKSHAKDMEDALKVVESCGIQHLVI
DLTEVHKSLFGAVEAELKAIGEWNEEKARLGDANTRARLRMTTLYAVANNYGYLVVGTDNAAEWHTGYFTKYGDGGVDLV
PLIHFTKGEVREMARLLGVPEEIITKAPSAGLWEGQTDESEMGTTYDMIDKYLKGEDIPEQDRKIIERLHERSHHKRQLA
IAPPKF
>Mature_246_residues
MQEKIDKLVQWLREQVASAGLNGAVVGISGGIDSAVVAHLIKRAFPDNSLGLIMPCKSHAKDMEDALKVVESCGIQHLVI
DLTEVHKSLFGAVEAELKAIGEWNEEKARLGDANTRARLRMTTLYAVANNYGYLVVGTDNAAEWHTGYFTKYGDGGVDLV
PLIHFTKGEVREMARLLGVPEEIITKAPSAGLWEGQTDESEMGTTYDMIDKYLKGEDIPEQDRKIIERLHERSHHKRQLA
IAPPKF

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=256, Percent_Identity=33.203125, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 27264; Mature: 27264

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEKIDKLVQWLREQVASAGLNGAVVGISGGIDSAVVAHLIKRAFPDNSLGLIMPCKSHA
CHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECHHHH
KDMEDALKVVESCGIQHLVIDLTEVHKSLFGAVEAELKAIGEWNEEKARLGDANTRARLR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHH
MTTLYAVANNYGYLVVGTDNAAEWHTGYFTKYGDGGVDLVPLIHFTKGEVREMARLLGVP
HHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHEECCHHHHHHHHHHHCCC
EEIITKAPSAGLWEGQTDESEMGTTYDMIDKYLKGEDIPEQDRKIIERLHERSHHKRQLA
HHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEC
IAPPKF
CCCCCC
>Mature Secondary Structure
MQEKIDKLVQWLREQVASAGLNGAVVGISGGIDSAVVAHLIKRAFPDNSLGLIMPCKSHA
CHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECHHHH
KDMEDALKVVESCGIQHLVIDLTEVHKSLFGAVEAELKAIGEWNEEKARLGDANTRARLR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHH
MTTLYAVANNYGYLVVGTDNAAEWHTGYFTKYGDGGVDLVPLIHFTKGEVREMARLLGVP
HHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHEECCHHHHHHHHHHHCCC
EEIITKAPSAGLWEGQTDESEMGTTYDMIDKYLKGEDIPEQDRKIIERLHERSHHKRQLA
HHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEC
IAPPKF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA