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The map label for this gene is greA [H]

Identifier: 138896117

GI number: 138896117

Start: 2583856

End: 2584332

Strand: Reverse

Name: greA [H]

Synonym: GTNG_2480

Alternate gene names: 138896117

Gene position: 2584332-2583856 (Counterclockwise)

Preceding gene: 138896118

Following gene: 138896116

Centisome position: 72.79

GC content: 46.96

Gene sequence:

>477_bases
ATGGCTAATGAGAAGCAGTATCCGATGACGAAAGAGGGAAAAGAGAAACTGGAGCAAGAACTTGAGTATTTAAAAACCGT
GAAACGAAAAGAAGTTGTCGAACGCATTAAAATTGCACGGGGGTTTGGTGATTTATCGGAAAACTCGGAATACGATGCAG
CAAAAGATGAGCAAGCGTTTGTCGAATCACGCATTCAAATGCTCGAAACGATGATTCGCAACGCGGTCATCATTGAAGAA
GACAAAGAAAACCCAGACGTCGTCTCACTCGGCAAATCGGTGACGTTCATTGAGCTTCCGGACGGTGAGGAAGAGACGTA
TACAATTGTCGGTAGCGCCGAAGCCGATCCGTTTGAAGGGAAAATTTCCAACGACTCACCGATTGCCAAATCGCTGATCG
GCCACCGCGTCGGCGACGAAGTGACTGTACAAACGCCAGGCGGGGAAATGCTCGTGAAAATTGTTGCAGTGAAATAA

Upstream 100 bases:

>100_bases
ACAGAGACAAAACAATATATTATATAACAAGTAAGCGTACGAACAGTTGTTTTTCTGTTCGTACGTTTTACATAATGAAG
AACATAGAGGAGTGAGATCA

Downstream 100 bases:

>100_bases
GCGCACCGTGCTGTCTTATGAAATGAGCGGCGGCTCAAGCCAAACGGACAGCGGTGTAAACAAAGCGGGAAAATCACTGC
CTTTGGTACGTTTCAAACGA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MANEKQYPMTKEGKEKLEQELEYLKTVKRKEVVERIKIARGFGDLSENSEYDAAKDEQAFVESRIQMLETMIRNAVIIEE
DKENPDVVSLGKSVTFIELPDGEEETYTIVGSAEADPFEGKISNDSPIAKSLIGHRVGDEVTVQTPGGEMLVKIVAVK

Sequences:

>Translated_158_residues
MANEKQYPMTKEGKEKLEQELEYLKTVKRKEVVERIKIARGFGDLSENSEYDAAKDEQAFVESRIQMLETMIRNAVIIEE
DKENPDVVSLGKSVTFIELPDGEEETYTIVGSAEADPFEGKISNDSPIAKSLIGHRVGDEVTVQTPGGEMLVKIVAVK
>Mature_157_residues
ANEKQYPMTKEGKEKLEQELEYLKTVKRKEVVERIKIARGFGDLSENSEYDAAKDEQAFVESRIQMLETMIRNAVIIEED
KENPDVVSLGKSVTFIELPDGEEETYTIVGSAEADPFEGKISNDSPIAKSLIGHRVGDEVTVQTPGGEMLVKIVAVK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=151, Percent_Identity=45.6953642384106, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI87082261, Length=149, Percent_Identity=35.5704697986577, Blast_Score=83, Evalue=7e-18,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17635; Mature: 17504

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANEKQYPMTKEGKEKLEQELEYLKTVKRKEVVERIKIARGFGDLSENSEYDAAKDEQAF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
VESRIQMLETMIRNAVIIEEDKENPDVVSLGKSVTFIELPDGEEETYTIVGSAEADPFEG
HHHHHHHHHHHHHCCEEEECCCCCCCEEECCCCEEEEECCCCCCCEEEEEECCCCCCCCC
KISNDSPIAKSLIGHRVGDEVTVQTPGGEMLVKIVAVK
CCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEEECC
>Mature Secondary Structure 
ANEKQYPMTKEGKEKLEQELEYLKTVKRKEVVERIKIARGFGDLSENSEYDAAKDEQAF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
VESRIQMLETMIRNAVIIEEDKENPDVVSLGKSVTFIELPDGEEETYTIVGSAEADPFEG
HHHHHHHHHHHHHCCEEEECCCCCCCEEECCCCEEEEECCCCCCCEEEEEECCCCCCCCC
KISNDSPIAKSLIGHRVGDEVTVQTPGGEMLVKIVAVK
CCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA