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The map label for this gene is nfo [H]

Identifier: 138896048

GI number: 138896048

Start: 2519765

End: 2520664

Strand: Reverse

Name: nfo [H]

Synonym: GTNG_2411

Alternate gene names: 138896048

Gene position: 2520664-2519765 (Counterclockwise)

Preceding gene: 138896049

Following gene: 138896046

Centisome position: 71.0

GC content: 49.67

Gene sequence:

>900_bases
ATGTTAAAAATCGGTTCACACGTTTCGATGAGCGGCAAAAAAATGCTGCTCGCCGCCAGTGAGGAGGCGGTATCGTACGG
AGCGAACACGTTTATGATTTACACCGGAGCGCCGCAAAACACAAGGCGCAAAGCCATCGAAGAGCTGAATATTGAAGCCG
GGCACGAGCATATGAAGGCGCACGGTATTGAGGACATTGTCGTCCACGCTCCGTATATTATCAATATCGGCAACACAAAA
AATCCCGATACGTTTGCGCTTGGCGTTAATTTTTTGCGCGCTGAAATCGAACGAACGGAAGCGATCGGGGCGAAGCAACT
TGTGTTGCACCCGGGCGCCCATGTCGGCGCTGGAGCGGAAGCCGGGCTCCGGCAGATCATCCGTGGACTGAATGAAGTGT
TGACGCGCGAGCAGACAGTTGAAATTGCTCTTGAAACGATGGCCGGTAAAGGATCGGAATGTGGGCGCACGTTTGAAGAG
CTGGCGTATATTATCGATGGCGTTGCCTATAACGACAAGCTGTCCGTCTGCTTTGACACATGTCATACGCATGATGCCGG
CTATGACATTGTTAACGATTTTGACGGCGTTCTTTCCGAATTCGACCGGGTGATCGGTCTTGAACGTCTGAAAGTGCTGC
ATATTAATGACAGCAAAAACCCGCGCGGCAGCCGGAAAGACCGCCACGAAAACATCGGTTTCGGTCATATCGGATTTCGT
GCACTGAATTACATCGTCCATCACCCGCAGCTTGCGGACATTCCGAAAATTTTAGAAACGCCGTATGTTGGAGAGGATAA
AAACAATAAAAAACCGCCGTACAAACATGAAATCGCTATGCTGCGGGCACAGACATTTGATGATCAGTTACTGGAAAAAA
TTGTTGCTGATACGCAATAA

Upstream 100 bases:

>100_bases
AAAAAGCTGCGCGCCGAGCTTGAGAAGAAGAAAAAGCGGCTGAGCCGCTTCAAAAAATCGTAGTCTTCGTTGTGTATGGA
GATAAAAGGGAGAGAAGGAC

Downstream 100 bases:

>100_bases
AAAAGAGATTCCAGCCTTATTTCACTTTTTCTGTACAAATTTTAAAAACAGTTCGTTCGCTTTCTTGGCGATCTCCGGCG
ACGTGATTTTGGCTAGTTCG

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLKIGSHVSMSGKKMLLAASEEAVSYGANTFMIYTGAPQNTRRKAIEELNIEAGHEHMKAHGIEDIVVHAPYIINIGNTK
NPDTFALGVNFLRAEIERTEAIGAKQLVLHPGAHVGAGAEAGLRQIIRGLNEVLTREQTVEIALETMAGKGSECGRTFEE
LAYIIDGVAYNDKLSVCFDTCHTHDAGYDIVNDFDGVLSEFDRVIGLERLKVLHINDSKNPRGSRKDRHENIGFGHIGFR
ALNYIVHHPQLADIPKILETPYVGEDKNNKKPPYKHEIAMLRAQTFDDQLLEKIVADTQ

Sequences:

>Translated_299_residues
MLKIGSHVSMSGKKMLLAASEEAVSYGANTFMIYTGAPQNTRRKAIEELNIEAGHEHMKAHGIEDIVVHAPYIINIGNTK
NPDTFALGVNFLRAEIERTEAIGAKQLVLHPGAHVGAGAEAGLRQIIRGLNEVLTREQTVEIALETMAGKGSECGRTFEE
LAYIIDGVAYNDKLSVCFDTCHTHDAGYDIVNDFDGVLSEFDRVIGLERLKVLHINDSKNPRGSRKDRHENIGFGHIGFR
ALNYIVHHPQLADIPKILETPYVGEDKNNKKPPYKHEIAMLRAQTFDDQLLEKIVADTQ
>Mature_299_residues
MLKIGSHVSMSGKKMLLAASEEAVSYGANTFMIYTGAPQNTRRKAIEELNIEAGHEHMKAHGIEDIVVHAPYIINIGNTK
NPDTFALGVNFLRAEIERTEAIGAKQLVLHPGAHVGAGAEAGLRQIIRGLNEVLTREQTVEIALETMAGKGSECGRTFEE
LAYIIDGVAYNDKLSVCFDTCHTHDAGYDIVNDFDGVLSEFDRVIGLERLKVLHINDSKNPRGSRKDRHENIGFGHIGFR
ALNYIVHHPQLADIPKILETPYVGEDKNNKKPPYKHEIAMLRAQTFDDQLLEKIVADTQ

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=294, Percent_Identity=34.3537414965986, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531193, Length=265, Percent_Identity=35.0943396226415, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6322735, Length=265, Percent_Identity=31.3207547169811, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 33132; Mature: 33132

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIGSHVSMSGKKMLLAASEEAVSYGANTFMIYTGAPQNTRRKAIEELNIEAGHEHMKA
CCCCCCCCCCCCCEEEEEECHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHH
HGIEDIVVHAPYIINIGNTKNPDTFALGVNFLRAEIERTEAIGAKQLVLHPGAHVGAGAE
CCCCCEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHH
AGLRQIIRGLNEVLTREQTVEIALETMAGKGSECGRTFEELAYIIDGVAYNDKLSVCFDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
CHTHDAGYDIVNDFDGVLSEFDRVIGLERLKVLHINDSKNPRGSRKDRHENIGFGHIGFR
HHCCCCCCHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCCCCCCCHHCCCCCCHHHHH
ALNYIVHHPQLADIPKILETPYVGEDKNNKKPPYKHEIAMLRAQTFDDQLLEKIVADTQ
HHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKIGSHVSMSGKKMLLAASEEAVSYGANTFMIYTGAPQNTRRKAIEELNIEAGHEHMKA
CCCCCCCCCCCCCEEEEEECHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHH
HGIEDIVVHAPYIINIGNTKNPDTFALGVNFLRAEIERTEAIGAKQLVLHPGAHVGAGAE
CCCCCEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHH
AGLRQIIRGLNEVLTREQTVEIALETMAGKGSECGRTFEELAYIIDGVAYNDKLSVCFDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
CHTHDAGYDIVNDFDGVLSEFDRVIGLERLKVLHINDSKNPRGSRKDRHENIGFGHIGFR
HHCCCCCCHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCCCCCCCHHCCCCCCHHHHH
ALNYIVHHPQLADIPKILETPYVGEDKNNKKPPYKHEIAMLRAQTFDDQLLEKIVADTQ
HHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA