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The map label for this gene is proI [H]

Identifier: 138895902

GI number: 138895902

Start: 2375199

End: 2376038

Strand: Direct

Name: proI [H]

Synonym: GTNG_2261

Alternate gene names: 138895902

Gene position: 2375199-2376038 (Clockwise)

Preceding gene: 138895899

Following gene: 138895903

Centisome position: 66.9

GC content: 59.05

Gene sequence:

>840_bases
ATGACAACAACTCGGTCCATCGCCTTCATCGGTGCCGGCTCGATGGCGGAAGCGCTCATCGCCGGCATGACGAAAACGTT
TTGCCATCCGACGCAAATCGTCGTGACAAACCGACAAAACCGCGCCCGGCTTGAAGAACTCAAGCGCACATACGGCGTCC
ATGCCGCCCAAGACGCACAACGCGCTGTCCAACATGCAGACATCGTTATTTTGGCCATGAAACCGAAAGATGTTGCCGAA
GCGATGGCCGCTGTTGCGCCGGCCATTCGTCCGAACCAGCTCATTCTCTCATTACTTGCCGGCGTCACAACCGAGACGAT
CGAACAGCTCGCTAGACGTGATGTCGCCGTCGTGCGCGCCATGCCAAACACATCCGCAGCCGTCGGCTTATCGGCCACCG
CGATCGCCGGGGGGCGTTTCGCCTCGGAGCAGCATCTTGAAACGGCAAAACAGCTGTTTGAAACGGTCGGCATTGTGACC
ATCGTGCCGGAGCGCGATTTGCATGCGGTCACCGGCCTATCGGGAAGCGGACCAGCGTACGTCTACTACTTGGTTGAAGC
GATGGAAAAGGCGGCCGCCGACATCGGCCTCGAGCGCGATGTGGCGAAAACGCTCATTTTACAGACGATCATCGGCGCAG
CGGAAATGTTAAAAGCGTCCGACAAACACCCGTCCGTCCTGCGCCGTGAGGTTACAAGCCCGGGTGGCACGACCGAAGCC
GGCATCAAAGTGCTTGAGCAATACCGGTTTCAAGAGGCTATCGCCGCCTGCATCCGCCGGGCGACCGCCCGTTCCGAAGA
ACTCGGCAACGCCTTGCTCACTGCGATCGCTGAATCATAA

Upstream 100 bases:

>100_bases
GCCAAAAAAGGTGGTACCGCGGAAACGAAGGTTTTCCGTCCTTTTGCAGGACGGGAAACCTTTTTCTTTTGCTGGACAAC
AAGGAAAGGAAGTGAATGCG

Downstream 100 bases:

>100_bases
CCGAACACACCGTTAAACGCCGAGGCTGTTTGTATCGAGTTGGGCAAATCATCGGACCGTTTCATTTTGTTTTTTGACCG
GCAGCATGCTATCATTCAGT

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase 2; P5CR 2 [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTTTRSIAFIGAGSMAEALIAGMTKTFCHPTQIVVTNRQNRARLEELKRTYGVHAAQDAQRAVQHADIVILAMKPKDVAE
AMAAVAPAIRPNQLILSLLAGVTTETIEQLARRDVAVVRAMPNTSAAVGLSATAIAGGRFASEQHLETAKQLFETVGIVT
IVPERDLHAVTGLSGSGPAYVYYLVEAMEKAAADIGLERDVAKTLILQTIIGAAEMLKASDKHPSVLRREVTSPGGTTEA
GIKVLEQYRFQEAIAACIRRATARSEELGNALLTAIAES

Sequences:

>Translated_279_residues
MTTTRSIAFIGAGSMAEALIAGMTKTFCHPTQIVVTNRQNRARLEELKRTYGVHAAQDAQRAVQHADIVILAMKPKDVAE
AMAAVAPAIRPNQLILSLLAGVTTETIEQLARRDVAVVRAMPNTSAAVGLSATAIAGGRFASEQHLETAKQLFETVGIVT
IVPERDLHAVTGLSGSGPAYVYYLVEAMEKAAADIGLERDVAKTLILQTIIGAAEMLKASDKHPSVLRREVTSPGGTTEA
GIKVLEQYRFQEAIAACIRRATARSEELGNALLTAIAES
>Mature_278_residues
TTTRSIAFIGAGSMAEALIAGMTKTFCHPTQIVVTNRQNRARLEELKRTYGVHAAQDAQRAVQHADIVILAMKPKDVAEA
MAAVAPAIRPNQLILSLLAGVTTETIEQLARRDVAVVRAMPNTSAAVGLSATAIAGGRFASEQHLETAKQLFETVGIVTI
VPERDLHAVTGLSGSGPAYVYYLVEAMEKAAADIGLERDVAKTLILQTIIGAAEMLKASDKHPSVLRREVTSPGGTTEAG
IKVLEQYRFQEAIAACIRRATARSEELGNALLTAIAES

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI198041662, Length=268, Percent_Identity=32.4626865671642, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI24797097, Length=268, Percent_Identity=34.3283582089552, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI24797095, Length=268, Percent_Identity=34.3283582089552, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI21361454, Length=267, Percent_Identity=32.5842696629214, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI1786585, Length=268, Percent_Identity=32.8358208955224, Blast_Score=150, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17569021, Length=269, Percent_Identity=34.9442379182156, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17540664, Length=282, Percent_Identity=26.9503546099291, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320861, Length=290, Percent_Identity=28.6206896551724, Blast_Score=116, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648116, Length=275, Percent_Identity=33.4545454545455, Blast_Score=150, Evalue=9e-37,
Organism=Drosophila melanogaster, GI21358587, Length=270, Percent_Identity=32.2222222222222, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24647700, Length=168, Percent_Identity=36.9047619047619, Blast_Score=117, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304 [H]

Pfam domain/function: PF03807 F420_oxidored [H]

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 29670; Mature: 29538

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00521 P5CR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTRSIAFIGAGSMAEALIAGMTKTFCHPTQIVVTNRQNRARLEELKRTYGVHAAQDAQ
CCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHH
RAVQHADIVILAMKPKDVAEAMAAVAPAIRPNQLILSLLAGVTTETIEQLARRDVAVVRA
HHHHHCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
MPNTSAAVGLSATAIAGGRFASEQHLETAKQLFETVGIVTIVPERDLHAVTGLSGSGPAY
CCCCCHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCHHHHCCCCCCCCHH
VYYLVEAMEKAAADIGLERDVAKTLILQTIIGAAEMLKASDKHPSVLRREVTSPGGTTEA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHH
GIKVLEQYRFQEAIAACIRRATARSEELGNALLTAIAES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTTRSIAFIGAGSMAEALIAGMTKTFCHPTQIVVTNRQNRARLEELKRTYGVHAAQDAQ
CCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHH
RAVQHADIVILAMKPKDVAEAMAAVAPAIRPNQLILSLLAGVTTETIEQLARRDVAVVRA
HHHHHCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
MPNTSAAVGLSATAIAGGRFASEQHLETAKQLFETVGIVTIVPERDLHAVTGLSGSGPAY
CCCCCHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCHHHHCCCCCCCCHH
VYYLVEAMEKAAADIGLERDVAKTLILQTIIGAAEMLKASDKHPSVLRREVTSPGGTTEA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHH
GIKVLEQYRFQEAIAACIRRATARSEELGNALLTAIAES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 11418582 [H]