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The map label for this gene is proB [H]

Identifier: 138895579

GI number: 138895579

Start: 2056522

End: 2057646

Strand: Direct

Name: proB [H]

Synonym: GTNG_1932

Alternate gene names: 138895579

Gene position: 2056522-2057646 (Clockwise)

Preceding gene: 138895565

Following gene: 138895580

Centisome position: 57.92

GC content: 55.56

Gene sequence:

>1125_bases
ATGAAACGGCAGCGCGTCGTTGTGAAAATCGGGAGCAGCTCACTCACCGATCCAAAAGGCGGCCTTTGCCATGATAAACT
GCTTGATCATGTTCAAGCGATCGCTTATATGAAACAGCTCGGCCATGACATCATTCTCATCACATCCGGTGCAGTTGCGG
CCGGCTTCGGGCCGTTAGGCTACCCAACGCGGCCGACGACGATCGCCGGCAAACAGGCGGCTGCTGCCGTCGGACAAAGC
TTATTGATGCAGGCGTACAGCGCACAGTTCGCCCAATTCGGCTTCACCGCCGCCCAGTTGTTGTTGACGCGCAGCGATTT
TTACAGCCGTGAGCGATTTCGCAACTTGTTTGCCACGATCACCACATTACTCGAACACGGCGCCGTGCCGATCATTAACG
AAAACGACTCCGTTTCCGTCGAAGAGTTGACGTTCGGCGACAATGATATGTTGTCAGCGCTTGTGGCAGGCTTCTTGCAT
GCCGATGCGCTCGTTTTGCTCACTGACATTAACGGGCTGTACGACGCCAATCCGAAAACGAACCCGCAGGCGAAAAAATA
CGCCTTTTTGCCAGAGATTACGAGCGAGATGCTCGCCTCAGCCGGCGGCAGCGGCTCGACTGTCGGCACCGGCGGCATGC
GCTCAAAGCTGTTAGCTGCGCAAAAAGCTCTCTCCTTCGGCGTCAGCGTCTTCATCGGCACGGGAAGCGGCAAAGAAAAA
CTGGCTGATATTTTAGCTGGAAAAGGTGACGGTACATACATCGGTGTTCCTTTTCCGAAACAGATGCAAATGCGCAAGCA
ATGGATCGCCTATCACGCCCCCGTTTCCGGCACAATCACTGTGGACAGCGGCGCCGAGGAAGCGCTGCTTGACCGCGGGA
AAAGTTTGCTGCCGGCCGGCGTCACGGCGGTTTCCGGCGACTTTCATGCGATGGATGTCGTCAATGTCATCAACGAAAAA
GGCGTTACCATCGGCCGCGGTCAAGTATATTACGCCGCCGCCGATTTGAAAAAAGTGAAAGGGCGGCCGAGCGAAGAAGC
GCGGCAATACTCTTACCTTCACCGCCCGGAAGTCATCCACCGCGACAATTGGGTCACTTTGCGAAAGGAGCGTGTCAAAA
GATGA

Upstream 100 bases:

>100_bases
TTTCAAAGAGGAGGAAAAGCGTTTTTTTATTGTCCTCTTCCCAGTTGTGGTCAAGAGACAGTGGAATAGACGACAGAAAA
AATCCAAAGGAGGGAATGCG

Downstream 100 bases:

>100_bases
ACGAATTGCTCGAAAAAGCCGAACGGTTGAAAACGGCGTCACAAACGTTGGCCATGCTGTCGACAGAAGAAAAAAACGCG
GCATTAGAACAGATTGCCAA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKRQRVVVKIGSSSLTDPKGGLCHDKLLDHVQAIAYMKQLGHDIILITSGAVAAGFGPLGYPTRPTTIAGKQAAAAVGQS
LLMQAYSAQFAQFGFTAAQLLLTRSDFYSRERFRNLFATITTLLEHGAVPIINENDSVSVEELTFGDNDMLSALVAGFLH
ADALVLLTDINGLYDANPKTNPQAKKYAFLPEITSEMLASAGGSGSTVGTGGMRSKLLAAQKALSFGVSVFIGTGSGKEK
LADILAGKGDGTYIGVPFPKQMQMRKQWIAYHAPVSGTITVDSGAEEALLDRGKSLLPAGVTAVSGDFHAMDVVNVINEK
GVTIGRGQVYYAAADLKKVKGRPSEEARQYSYLHRPEVIHRDNWVTLRKERVKR

Sequences:

>Translated_374_residues
MKRQRVVVKIGSSSLTDPKGGLCHDKLLDHVQAIAYMKQLGHDIILITSGAVAAGFGPLGYPTRPTTIAGKQAAAAVGQS
LLMQAYSAQFAQFGFTAAQLLLTRSDFYSRERFRNLFATITTLLEHGAVPIINENDSVSVEELTFGDNDMLSALVAGFLH
ADALVLLTDINGLYDANPKTNPQAKKYAFLPEITSEMLASAGGSGSTVGTGGMRSKLLAAQKALSFGVSVFIGTGSGKEK
LADILAGKGDGTYIGVPFPKQMQMRKQWIAYHAPVSGTITVDSGAEEALLDRGKSLLPAGVTAVSGDFHAMDVVNVINEK
GVTIGRGQVYYAAADLKKVKGRPSEEARQYSYLHRPEVIHRDNWVTLRKERVKR
>Mature_374_residues
MKRQRVVVKIGSSSLTDPKGGLCHDKLLDHVQAIAYMKQLGHDIILITSGAVAAGFGPLGYPTRPTTIAGKQAAAAVGQS
LLMQAYSAQFAQFGFTAAQLLLTRSDFYSRERFRNLFATITTLLEHGAVPIINENDSVSVEELTFGDNDMLSALVAGFLH
ADALVLLTDINGLYDANPKTNPQAKKYAFLPEITSEMLASAGGSGSTVGTGGMRSKLLAAQKALSFGVSVFIGTGSGKEK
LADILAGKGDGTYIGVPFPKQMQMRKQWIAYHAPVSGTITVDSGAEEALLDRGKSLLPAGVTAVSGDFHAMDVVNVINEK
GVTIGRGQVYYAAADLKKVKGRPSEEARQYSYLHRPEVIHRDNWVTLRKERVKR

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=285, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI62912457, Length=283, Percent_Identity=33.2155477031802, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=42.2343324250681, Blast_Score=268, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17569967, Length=267, Percent_Identity=35.9550561797753, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17569969, Length=267, Percent_Identity=35.9550561797753, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=33.7349397590361, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321822, Length=414, Percent_Identity=32.6086956521739, Blast_Score=213, Evalue=6e-56,
Organism=Drosophila melanogaster, GI21357643, Length=282, Percent_Identity=35.4609929078014, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40130; Mature: 40130

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQRVVVKIGSSSLTDPKGGLCHDKLLDHVQAIAYMKQLGHDIILITSGAVAAGFGPLG
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCC
YPTRPTTIAGKQAAAAVGQSLLMQAYSAQFAQFGFTAAQLLLTRSDFYSRERFRNLFATI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTLLEHGAVPIINENDSVSVEELTFGDNDMLSALVAGFLHADALVLLTDINGLYDANPKT
HHHHHCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCC
NPQAKKYAFLPEITSEMLASAGGSGSTVGTGGMRSKLLAAQKALSFGVSVFIGTGSGKEK
CCCHHHEEECHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHH
LADILAGKGDGTYIGVPFPKQMQMRKQWIAYHAPVSGTITVDSGAEEALLDRGKSLLPAG
HHHHHCCCCCCCEEECCCCHHHHHHHHHHHEECCCCCEEEECCCCHHHHHHCCCCCCCCC
VTAVSGDFHAMDVVNVINEKGVTIGRGQVYYAAADLKKVKGRPSEEARQYSYLHRPEVIH
CEEECCCHHHHHHHHHHHHCCCEEECCEEEEEEHHHHHHCCCCHHHHHHHHHHCCCCEEE
RDNWVTLRKERVKR
CCCCEEEHHHHCCC
>Mature Secondary Structure
MKRQRVVVKIGSSSLTDPKGGLCHDKLLDHVQAIAYMKQLGHDIILITSGAVAAGFGPLG
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCC
YPTRPTTIAGKQAAAAVGQSLLMQAYSAQFAQFGFTAAQLLLTRSDFYSRERFRNLFATI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTLLEHGAVPIINENDSVSVEELTFGDNDMLSALVAGFLHADALVLLTDINGLYDANPKT
HHHHHCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCC
NPQAKKYAFLPEITSEMLASAGGSGSTVGTGGMRSKLLAAQKALSFGVSVFIGTGSGKEK
CCCHHHEEECHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHH
LADILAGKGDGTYIGVPFPKQMQMRKQWIAYHAPVSGTITVDSGAEEALLDRGKSLLPAG
HHHHHCCCCCCCEEECCCCHHHHHHHHHHHEECCCCCEEEECCCCHHHHHHCCCCCCCCC
VTAVSGDFHAMDVVNVINEKGVTIGRGQVYYAAADLKKVKGRPSEEARQYSYLHRPEVIH
CEEECCCHHHHHHHHHHHHCCCEEECCEEEEEEHHHHHHCCCCHHHHHHHHHHCCCCEEE
RDNWVTLRKERVKR
CCCCEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA