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The map label for this gene is gmuA [H]

Identifier: 138895405

GI number: 138895405

Start: 1850316

End: 1850648

Strand: Reverse

Name: gmuA [H]

Synonym: GTNG_1749

Alternate gene names: 138895405

Gene position: 1850648-1850316 (Counterclockwise)

Preceding gene: 138895406

Following gene: 138895404

Centisome position: 52.13

GC content: 42.94

Gene sequence:

>333_bases
ATGGAAAAAGTGGACATACACCAACTTACGAATGAACAAATTGCTTTTCACATCATTTTGCATAGCGGCAACGCTCGCAG
TAAAATTGTCCAAGCTATCCGTGAATACCGTTCAGGCCAAATTGAGGAATCAGAACGATTGATCGAAGAAGCGGAACAAG
AGTTAGGTGTCGCGCACGATGTCCATTTTCAACTTATCCAAAAGGAAGCGGGCGGAAAGCCAACAACGATCACCTTGCTT
CTTATGCATGCGGAAGACCATGTTATGTCAACGTTGGCGATGAAAGAGATTGTGAAAGAGATGCTTGCTTTGTTAAAGGA
GCGGAATATATAA

Upstream 100 bases:

>100_bases
TATGGCATTCAAGTGGACGTCATTGATATGGTGGCCTATGGGATGGCGGATGGAAAAAAAGTGTACGAACAAGCGCTGAA
ATTAATGGAGGAAAAATAAG

Downstream 100 bases:

>100_bases
AAAGGAGGGCTGTCAAATGAACGGTGCTTTATTTGAGAAATTGAGCAAAGTGCTCGTTCCGGTCGCTGGAAAGTTGAATA
ACAGTCGTTATTTGCAAGTG

Product: PTS system cellobiose-specific transporter subunit IIA

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: Glucomannan utilization protein A; PTS system oligo-beta-mannoside-specific EIIA component [H]

Number of amino acids: Translated: 110; Mature: 110

Protein sequence:

>110_residues
MEKVDIHQLTNEQIAFHIILHSGNARSKIVQAIREYRSGQIEESERLIEEAEQELGVAHDVHFQLIQKEAGGKPTTITLL
LMHAEDHVMSTLAMKEIVKEMLALLKERNI

Sequences:

>Translated_110_residues
MEKVDIHQLTNEQIAFHIILHSGNARSKIVQAIREYRSGQIEESERLIEEAEQELGVAHDVHFQLIQKEAGGKPTTITLL
LMHAEDHVMSTLAMKEIVKEMLALLKERNI
>Mature_110_residues
MEKVDIHQLTNEQIAFHIILHSGNARSKIVQAIREYRSGQIEESERLIEEAEQELGVAHDVHFQLIQKEAGGKPTTITLL
LMHAEDHVMSTLAMKEIVKEMLALLKERNI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1447

COG function: function code G; Phosphotransferase system cellobiose-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788031, Length=93, Percent_Identity=29.0322580645161, Blast_Score=60, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003188 [H]

Pfam domain/function: PF02255 PTS_IIA [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 12565; Mature: 12565

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS51095 PTS_EIIA_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVDIHQLTNEQIAFHIILHSGNARSKIVQAIREYRSGQIEESERLIEEAEQELGVAHD
CCCCHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHH
VHFQLIQKEAGGKPTTITLLLMHAEDHVMSTLAMKEIVKEMLALLKERNI
HHHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKVDIHQLTNEQIAFHIILHSGNARSKIVQAIREYRSGQIEESERLIEEAEQELGVAHD
CCCCHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHH
VHFQLIQKEAGGKPTTITLLLMHAEDHVMSTLAMKEIVKEMLALLKERNI
HHHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]