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The map label for this gene is topA [H]

Identifier: 138895358

GI number: 138895358

Start: 1791467

End: 1793623

Strand: Reverse

Name: topA [H]

Synonym: GTNG_1702

Alternate gene names: 138895358

Gene position: 1793623-1791467 (Counterclockwise)

Preceding gene: 138895359

Following gene: 138895356

Centisome position: 50.52

GC content: 52.16

Gene sequence:

>2157_bases
ATGAAAGTCATTATTGCAGAAAAACCAGATCAAGGGGCAACACTCGCCTCCATTTTTCGCACGAAAAAACGGCAAGGTTA
TATCGAAATTTTGCCAAACGACTTGTTTCCGGACGGAGCGTACATGACATGGGCGATCGGCCATCTTTGCCAGCTTGCGC
CGCCTGAGCGGTATCGCTCGGAATGGAAGCGGTGGACGTTGAAGGCGCTGCCCATCATTCCAGAACGATTTGAATACGAA
GTGGAAAAAGCGAAAGCAAAGCAGTTCAAAGTCGTGAAAGAACTGCTGCGGAAGCCGGAAGTGAAGGAAATCATTCATGC
CGGCGACGCCGGGCGTGAAGGGGAGCTCATTGTGCGCAACATCATCCGCCTGAGCGGCGTCAATAAACCGATGAAGCGGC
TTTGGCTGTCGTCATTGACCCCGAAAGCGATTGAAGAAGGGTTTCGCCGCTTGCTCGACGAGCGGACGACGCGTCCATTG
TATGAGGAAGCGTACGCCCGCGCCTGCGCGGATTGGCTCGTCGGCATGAACGCTTCACGAGCCTACAGCCTGTTGCTTAA
GGAGCGGGGGATGAACGATGTGTTCTCCGTTGGGCGCGTGCAAACGCCGACATTGGCCTTAATTGTCCGGCGTGAGCGGG
AAATTGAACAGTTTCGCCCTGAGCCGTTTTGGGAAGTGGTCGCTTCGTTTGTCATCGGCGATAAGCAGTATGAAGGAAAA
TGGATAGATGAAAACGGGCAAACGCGTCTCAAGGACGAAGCGATGGCAAAAAACATCGCCGCTTTCTGTCGGGGGAAGCA
GGCGGTAGTCGCCGACGTCCGTACGGATCGGAAAACGTTCCAACCGCCGCTGTTGTTCAACCTGTCGACACTGCAGGCGA
CAGCGAACAAACTCTATCGCTTTTCCCCCAAAAAAACGCTCGATGTGTTGCAGTCGCTCTATCAAAAAGGAATCGTCTCG
TATCCACGCTCTGATTCGATGCATGTGACCAGGGGAGAGGCGGAAACGTTCCCGGCTATTTTACAAAAGCTGTCGGCGTT
TTCAGCGTACGCTTCGTATTTTCCGCTGCCGCAGCCTTCGATTTTACATAACAAACGTTATGTAAATGAAAAGAAAGTGA
CTGACCATTATGCCATCATTCCGACCGAACAAGTCGTTGACCCAGAAAAGCTGTCGGCCGATGAGCGGAAAGTGTATGAT
TTGATCGTCCGCCGACTCATCGCCGCCCATCACCAGGCGGCGGTTATCGATTATACAACGGTGGCGACGCTTGTTGATGG
ACGTGCTCGTTTCATCACAAAAGGAAGACAAGTCATTGAGGAAGGATGGCGCGCTGTCATTCGCCCACATGAGGAGGAAA
AGGAAACGGTGCTTCCGCTACTCTGCCAAGGTGAAGCCGGCGAAGTGGCGGACATCCAAGTAAAAGAAGGAAAAACGGAA
CCTCCGAAACGGTATACGGAAGGGCAACTCATTACGCTCATGAAAACAGCCGGGAAGTTTTTAGATGATGAAGCGCTTGA
AAAAGTGCTCGCCAAAACGGAAGGGCTCGGCACAGAGGCGACAAGGGCGGCGATCATTACGATGCTGAAAGAGCGGAAGT
ATATCGAGGTGAAAAACAACCTTGTGTATGCGACGGACAAGGCCAAAGTATTGATCGAGGCGCTCGGTGGCACGATTCTC
GCTTCACCGGAAATGACGGCAAAATGGGAACAGCGTTTAAGCGAAATTGGTGAAGGAGCGGCCTCCTCCGCCGCGTTTAT
GGAACAGGCGAAAAAGCTGGCGCAAAAAGTGGTTGCTGATGCCATCGCCGCTGCGCCAAGCTGGAATTTCAGCGGGCTTG
ACACCGCTTCGATCCAGCGGACGAAAACGAAAAAAACGGTTGGTGAACCGCTCGGGCGCTGTCCGCTTTGCGGCGGAACC
GTCGTTGACAAAGGAACGTTATACGGCTGTGACCAATATGTGAAAACGAGCTGCTCGTTTACGGTCTCAAAACGTATTCT
CGGCAAGTCGATTTCGGCGGCGAATATGAAAAAGCTGCTTGCGAAGGGCAAAACGAATGTCATCAAAGGATTCAAAAAAG
GTGGAAAAACGTTTGACGCTGTCCTAGTGTGGGATGAAAACGATAAGAAGCTGTCATTTCAGTTTCCGAAACGGTGA

Upstream 100 bases:

>100_bases
GCGGGCGGTGCGAGCATTATCTTTCTCGTTGTCCCGTGAGCAATGGTTCGCCATTTTGCAAAGTTCGATGGGGCATGATG
TACCGTAAGGAGAGAAGTCA

Downstream 100 bases:

>100_bases
CGGAAGGCAGATAAAGCATGCTGCTTGTGAAAAAGCGTTTCGCAAAAAGGCGGGCCGTGAACGGCCTGCTTTTTTTCATT
TTTGAATCCGCTCGCTAACC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MKVIIAEKPDQGATLASIFRTKKRQGYIEILPNDLFPDGAYMTWAIGHLCQLAPPERYRSEWKRWTLKALPIIPERFEYE
VEKAKAKQFKVVKELLRKPEVKEIIHAGDAGREGELIVRNIIRLSGVNKPMKRLWLSSLTPKAIEEGFRRLLDERTTRPL
YEEAYARACADWLVGMNASRAYSLLLKERGMNDVFSVGRVQTPTLALIVRREREIEQFRPEPFWEVVASFVIGDKQYEGK
WIDENGQTRLKDEAMAKNIAAFCRGKQAVVADVRTDRKTFQPPLLFNLSTLQATANKLYRFSPKKTLDVLQSLYQKGIVS
YPRSDSMHVTRGEAETFPAILQKLSAFSAYASYFPLPQPSILHNKRYVNEKKVTDHYAIIPTEQVVDPEKLSADERKVYD
LIVRRLIAAHHQAAVIDYTTVATLVDGRARFITKGRQVIEEGWRAVIRPHEEEKETVLPLLCQGEAGEVADIQVKEGKTE
PPKRYTEGQLITLMKTAGKFLDDEALEKVLAKTEGLGTEATRAAIITMLKERKYIEVKNNLVYATDKAKVLIEALGGTIL
ASPEMTAKWEQRLSEIGEGAASSAAFMEQAKKLAQKVVADAIAAAPSWNFSGLDTASIQRTKTKKTVGEPLGRCPLCGGT
VVDKGTLYGCDQYVKTSCSFTVSKRILGKSISAANMKKLLAKGKTNVIKGFKKGGKTFDAVLVWDENDKKLSFQFPKR

Sequences:

>Translated_718_residues
MKVIIAEKPDQGATLASIFRTKKRQGYIEILPNDLFPDGAYMTWAIGHLCQLAPPERYRSEWKRWTLKALPIIPERFEYE
VEKAKAKQFKVVKELLRKPEVKEIIHAGDAGREGELIVRNIIRLSGVNKPMKRLWLSSLTPKAIEEGFRRLLDERTTRPL
YEEAYARACADWLVGMNASRAYSLLLKERGMNDVFSVGRVQTPTLALIVRREREIEQFRPEPFWEVVASFVIGDKQYEGK
WIDENGQTRLKDEAMAKNIAAFCRGKQAVVADVRTDRKTFQPPLLFNLSTLQATANKLYRFSPKKTLDVLQSLYQKGIVS
YPRSDSMHVTRGEAETFPAILQKLSAFSAYASYFPLPQPSILHNKRYVNEKKVTDHYAIIPTEQVVDPEKLSADERKVYD
LIVRRLIAAHHQAAVIDYTTVATLVDGRARFITKGRQVIEEGWRAVIRPHEEEKETVLPLLCQGEAGEVADIQVKEGKTE
PPKRYTEGQLITLMKTAGKFLDDEALEKVLAKTEGLGTEATRAAIITMLKERKYIEVKNNLVYATDKAKVLIEALGGTIL
ASPEMTAKWEQRLSEIGEGAASSAAFMEQAKKLAQKVVADAIAAAPSWNFSGLDTASIQRTKTKKTVGEPLGRCPLCGGT
VVDKGTLYGCDQYVKTSCSFTVSKRILGKSISAANMKKLLAKGKTNVIKGFKKGGKTFDAVLVWDENDKKLSFQFPKR
>Mature_718_residues
MKVIIAEKPDQGATLASIFRTKKRQGYIEILPNDLFPDGAYMTWAIGHLCQLAPPERYRSEWKRWTLKALPIIPERFEYE
VEKAKAKQFKVVKELLRKPEVKEIIHAGDAGREGELIVRNIIRLSGVNKPMKRLWLSSLTPKAIEEGFRRLLDERTTRPL
YEEAYARACADWLVGMNASRAYSLLLKERGMNDVFSVGRVQTPTLALIVRREREIEQFRPEPFWEVVASFVIGDKQYEGK
WIDENGQTRLKDEAMAKNIAAFCRGKQAVVADVRTDRKTFQPPLLFNLSTLQATANKLYRFSPKKTLDVLQSLYQKGIVS
YPRSDSMHVTRGEAETFPAILQKLSAFSAYASYFPLPQPSILHNKRYVNEKKVTDHYAIIPTEQVVDPEKLSADERKVYD
LIVRRLIAAHHQAAVIDYTTVATLVDGRARFITKGRQVIEEGWRAVIRPHEEEKETVLPLLCQGEAGEVADIQVKEGKTE
PPKRYTEGQLITLMKTAGKFLDDEALEKVLAKTEGLGTEATRAAIITMLKERKYIEVKNNLVYATDKAKVLIEALGGTIL
ASPEMTAKWEQRLSEIGEGAASSAAFMEQAKKLAQKVVADAIAAAPSWNFSGLDTASIQRTKTKKTVGEPLGRCPLCGGT
VVDKGTLYGCDQYVKTSCSFTVSKRILGKSISAANMKKLLAKGKTNVIKGFKKGGKTFDAVLVWDENDKKLSFQFPKR

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=676, Percent_Identity=25, Blast_Score=146, Evalue=6e-35,
Organism=Escherichia coli, GI1788061, Length=610, Percent_Identity=35.0819672131148, Blast_Score=337, Evalue=2e-93,
Organism=Escherichia coli, GI1787529, Length=527, Percent_Identity=28.0834914611006, Blast_Score=139, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17555378, Length=620, Percent_Identity=26.4516129032258, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI32563869, Length=666, Percent_Identity=23.5735735735736, Blast_Score=122, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=431, Percent_Identity=29.0023201856148, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24585251, Length=642, Percent_Identity=26.01246105919, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24640096, Length=622, Percent_Identity=23.9549839228296, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 80737; Mature: 80737

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIIAEKPDQGATLASIFRTKKRQGYIEILPNDLFPDGAYMTWAIGHLCQLAPPERYRS
CEEEEECCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHH
EWKRWTLKALPIIPERFEYEVEKAKAKQFKVVKELLRKPEVKEIIHAGDAGREGELIVRN
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHH
IIRLSGVNKPMKRLWLSSLTPKAIEEGFRRLLDERTTRPLYEEAYARACADWLVGMNASR
HHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHH
AYSLLLKERGMNDVFSVGRVQTPTLALIVRREREIEQFRPEPFWEVVASFVIGDKQYEGK
HHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
WIDENGQTRLKDEAMAKNIAAFCRGKQAVVADVRTDRKTFQPPLLFNLSTLQATANKLYR
EECCCCCCHHHHHHHHHHHHHHHCCCHHEEEHHHHCHHHCCCCCEEEHHHHHHHHHHHHC
FSPKKTLDVLQSLYQKGIVSYPRSDSMHVTRGEAETFPAILQKLSAFSAYASYFPLPQPS
CCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
ILHNKRYVNEKKVTDHYAIIPTEQVVDPEKLSADERKVYDLIVRRLIAAHHQAAVIDYTT
HHHCCHHCCCHHCCCCEEECCHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHEEHHHH
VATLVDGRARFITKGRQVIEEGWRAVIRPHEEEKETVLPLLCQGEAGEVADIQVKEGKTE
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEECCCCCCEEEEEEECCCCC
PPKRYTEGQLITLMKTAGKFLDDEALEKVLAKTEGLGTEATRAAIITMLKERKYIEVKNN
CCCCCCCCCEEHHHHHHCHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECC
LVYATDKAKVLIEALGGTILASPEMTAKWEQRLSEIGEGAASSAAFMEQAKKLAQKVVAD
EEEECCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AIAAAPSWNFSGLDTASIQRTKTKKTVGEPLGRCPLCGGTVVDKGTLYGCDQYVKTSCSF
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEECCHHHHHCCCCH
TVSKRILGKSISAANMKKLLAKGKTNVIKGFKKGGKTFDAVLVWDENDKKLSFQFPKR
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCC
>Mature Secondary Structure
MKVIIAEKPDQGATLASIFRTKKRQGYIEILPNDLFPDGAYMTWAIGHLCQLAPPERYRS
CEEEEECCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHH
EWKRWTLKALPIIPERFEYEVEKAKAKQFKVVKELLRKPEVKEIIHAGDAGREGELIVRN
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHH
IIRLSGVNKPMKRLWLSSLTPKAIEEGFRRLLDERTTRPLYEEAYARACADWLVGMNASR
HHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHH
AYSLLLKERGMNDVFSVGRVQTPTLALIVRREREIEQFRPEPFWEVVASFVIGDKQYEGK
HHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
WIDENGQTRLKDEAMAKNIAAFCRGKQAVVADVRTDRKTFQPPLLFNLSTLQATANKLYR
EECCCCCCHHHHHHHHHHHHHHHCCCHHEEEHHHHCHHHCCCCCEEEHHHHHHHHHHHHC
FSPKKTLDVLQSLYQKGIVSYPRSDSMHVTRGEAETFPAILQKLSAFSAYASYFPLPQPS
CCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
ILHNKRYVNEKKVTDHYAIIPTEQVVDPEKLSADERKVYDLIVRRLIAAHHQAAVIDYTT
HHHCCHHCCCHHCCCCEEECCHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHEEHHHH
VATLVDGRARFITKGRQVIEEGWRAVIRPHEEEKETVLPLLCQGEAGEVADIQVKEGKTE
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEECCCCCCEEEEEEECCCCC
PPKRYTEGQLITLMKTAGKFLDDEALEKVLAKTEGLGTEATRAAIITMLKERKYIEVKNN
CCCCCCCCCEEHHHHHHCHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECC
LVYATDKAKVLIEALGGTILASPEMTAKWEQRLSEIGEGAASSAAFMEQAKKLAQKVVAD
EEEECCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AIAAAPSWNFSGLDTASIQRTKTKKTVGEPLGRCPLCGGTVVDKGTLYGCDQYVKTSCSF
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEECCHHHHHCCCCH
TVSKRILGKSISAANMKKLLAKGKTNVIKGFKKGGKTFDAVLVWDENDKKLSFQFPKR
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1329032 [H]