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The map label for this gene is cbiH [H]

Identifier: 138895350

GI number: 138895350

Start: 1784032

End: 1785822

Strand: Reverse

Name: cbiH [H]

Synonym: GTNG_1694

Alternate gene names: 138895350

Gene position: 1785822-1784032 (Counterclockwise)

Preceding gene: 138895351

Following gene: 138895349

Centisome position: 50.3

GC content: 58.46

Gene sequence:

>1791_bases
ATGATGAGCGGCAAATTGCTCATTGTCGGCTTTGGCCCGGGAAGCGTCGACCATATGACGAAACGGGCTCGGGAAGCGAT
TGAAGAAAGCGACGTCATCATCGGCTACAAAACGTACATCGAACTTGTGCGCGATCTGATTGTCGGAAAGGAAATCATCA
GCACGGGCATGACGGAAGAGGTGAGCCGCGCCCAAGCGGCGGTGAAATGGGCGGAGCGCGGCAAAACGGTCGCCGTCATT
TCAAGCGGTGACGCCGGGTTGTACGGCATGGCCGGGCTCGTGTATGAAGTGCTGATCGAAAAAGGGTGGACGAAAGACAG
CTCGATTGAAGTCGAAGTCATCCCCGGCATTTCCGCGATCCATTCGTGCGCCGCGCTGCTTGGCGCACCGATTATGCACG
ATGCGTGCACGATCAGCTTGAGCGACCATTTGACGCCGTGGGAGCTGATTGAAAAGCGGATTGACGCCGCCGCCGCGGCC
GATTTTGTCATCGCGCTTTACAACCCGAAAAGCGGACGGCGCACTAGACAGATTGTGGAAGCACAGCGCATCCTGCTCCG
CCATCGGTCTCCGGAAACCCCGGTCGGTCTGGTGAAAAGCGCGTACCGCGAGCGGCAGCATATCGTCCTCACCGACTTGG
CGCATATGCTTGACCATGAGATCGGCATGTTGACGACGGTGATTATCGGCAACTCAACGACATTCGTCCATGACGGCGTG
ATGATCACGCCGCGCGGCTACCAGCGCAAGTATCAACTAGCAGCCGCCGTCCAGCCGCTCAAACCGCATGAACGGCTGCG
CAAAGAAGCGGAGCCGTGGGCGCTTGACCAGACGAAACCGCTGGAAGATGAGGACGAAGGAAGCACGGGGGCATCAGGAC
CGTCCGAAGCGGCTGTCCTCGCCGGGGACAACCAGAAGGATCGTGCTTTCACCGCCTTGGCCACCGCGGTTCGACCGCCG
TCTGTTGCCGCGGCCACGAAGGAGGCCGAGCCAACGCCGCTGGCTGCCGTTTTGCCGCCGCTTGCTGCACCGCTGGCAAA
AACAGCCAAACGGCAGCCGACCGCCGCGCCAAAACGGGTGAAAGAAACAGCCGAAGACGCGCTCGCCGCTTTAGAGAGAC
GGAAAACAAGAAAGACGGCGGCCGTGTTGTTGGAAGCGGCGGTGAGCCCGGGCGTGGCCAATAAACAGTTCACCCCGGAA
CAGCTCATCGTGCTTGCGGAAGTGGTAGGGATGGATGGAAAAATGACCTACACACCGGATCATTATATGAAAATCGAACG
GTTGACAGAGGACCCAGATGGGCTTGTGGCCCGTCTCGTTGACGCCGGACTGATCGTGATGCCGGTCGGTGATGTGCTGA
CGGTGAAGGCATGTGATTTTTGCGATGGAGAGAAAAAAGACGCCATTCCATACGCGGAGGAATTGGCGCGCCGGTTTGGC
GGCATGGCGCTGCCGAAAGAGTTGAAGCTTGGCATCAACGGCTGCGGGATGGCGTGCTACGGCGCGGTGCACGAAGACAT
CGGGCTTGTGTACCGGAAAGGGAAATTTGATTTGTTTTTGGGCGGGAAAACGGTCGGCCGCAACGCCCATCCCGGCCAGC
TCGTCGCGGAAGGCATTCCGCCGGAAGAAATTGTGGCGGTTATGGCAGAAATCATCGAACAGTATCAAGAACAAGCACAT
CCAAATGAACGGTTTCATAAGTTTTTCCGCCGCGTGAAGCAAGTCGGACGTTTTTCACACGAAGAGGCAAAACCGGCCGC
GTCCATCGAAATGCCGGCTTGCGGTGAGTGA

Upstream 100 bases:

>100_bases
AAAGGAAAAGAACGTGCCCCGATGTTTTGTACCATGAAGGGAAATAAGCGGCGGCGAATTCGTTATGGAAAGTGGGCCTA
ATTGGCAAAGGAGGGGGATC

Downstream 100 bases:

>100_bases
CGGAAGCATGCGTTATATAAGTGCAGCTTGAACGTCTGCAGATGTCGTTGGTGTCGAAGCGACGCATCTTTTTCCGACAG
TCGATCGAATAACCGGCTGG

Product: precorrin-3 methylase

Products: 2S-adenosyl-L-homocysteine; precorrin; sirohydrochlorin; NADH; Proton; siroheme [C]

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MMSGKLLIVGFGPGSVDHMTKRAREAIEESDVIIGYKTYIELVRDLIVGKEIISTGMTEEVSRAQAAVKWAERGKTVAVI
SSGDAGLYGMAGLVYEVLIEKGWTKDSSIEVEVIPGISAIHSCAALLGAPIMHDACTISLSDHLTPWELIEKRIDAAAAA
DFVIALYNPKSGRRTRQIVEAQRILLRHRSPETPVGLVKSAYRERQHIVLTDLAHMLDHEIGMLTTVIIGNSTTFVHDGV
MITPRGYQRKYQLAAAVQPLKPHERLRKEAEPWALDQTKPLEDEDEGSTGASGPSEAAVLAGDNQKDRAFTALATAVRPP
SVAAATKEAEPTPLAAVLPPLAAPLAKTAKRQPTAAPKRVKETAEDALAALERRKTRKTAAVLLEAAVSPGVANKQFTPE
QLIVLAEVVGMDGKMTYTPDHYMKIERLTEDPDGLVARLVDAGLIVMPVGDVLTVKACDFCDGEKKDAIPYAEELARRFG
GMALPKELKLGINGCGMACYGAVHEDIGLVYRKGKFDLFLGGKTVGRNAHPGQLVAEGIPPEEIVAVMAEIIEQYQEQAH
PNERFHKFFRRVKQVGRFSHEEAKPAASIEMPACGE

Sequences:

>Translated_596_residues
MMSGKLLIVGFGPGSVDHMTKRAREAIEESDVIIGYKTYIELVRDLIVGKEIISTGMTEEVSRAQAAVKWAERGKTVAVI
SSGDAGLYGMAGLVYEVLIEKGWTKDSSIEVEVIPGISAIHSCAALLGAPIMHDACTISLSDHLTPWELIEKRIDAAAAA
DFVIALYNPKSGRRTRQIVEAQRILLRHRSPETPVGLVKSAYRERQHIVLTDLAHMLDHEIGMLTTVIIGNSTTFVHDGV
MITPRGYQRKYQLAAAVQPLKPHERLRKEAEPWALDQTKPLEDEDEGSTGASGPSEAAVLAGDNQKDRAFTALATAVRPP
SVAAATKEAEPTPLAAVLPPLAAPLAKTAKRQPTAAPKRVKETAEDALAALERRKTRKTAAVLLEAAVSPGVANKQFTPE
QLIVLAEVVGMDGKMTYTPDHYMKIERLTEDPDGLVARLVDAGLIVMPVGDVLTVKACDFCDGEKKDAIPYAEELARRFG
GMALPKELKLGINGCGMACYGAVHEDIGLVYRKGKFDLFLGGKTVGRNAHPGQLVAEGIPPEEIVAVMAEIIEQYQEQAH
PNERFHKFFRRVKQVGRFSHEEAKPAASIEMPACGE
>Mature_596_residues
MMSGKLLIVGFGPGSVDHMTKRAREAIEESDVIIGYKTYIELVRDLIVGKEIISTGMTEEVSRAQAAVKWAERGKTVAVI
SSGDAGLYGMAGLVYEVLIEKGWTKDSSIEVEVIPGISAIHSCAALLGAPIMHDACTISLSDHLTPWELIEKRIDAAAAA
DFVIALYNPKSGRRTRQIVEAQRILLRHRSPETPVGLVKSAYRERQHIVLTDLAHMLDHEIGMLTTVIIGNSTTFVHDGV
MITPRGYQRKYQLAAAVQPLKPHERLRKEAEPWALDQTKPLEDEDEGSTGASGPSEAAVLAGDNQKDRAFTALATAVRPP
SVAAATKEAEPTPLAAVLPPLAAPLAKTAKRQPTAAPKRVKETAEDALAALERRKTRKTAAVLLEAAVSPGVANKQFTPE
QLIVLAEVVGMDGKMTYTPDHYMKIERLTEDPDGLVARLVDAGLIVMPVGDVLTVKACDFCDGEKKDAIPYAEELARRFG
GMALPKELKLGINGCGMACYGAVHEDIGLVYRKGKFDLFLGGKTVGRNAHPGQLVAEGIPPEEIVAVMAEIIEQYQEQAH
PNERFHKFFRRVKQVGRFSHEEAKPAASIEMPACGE

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.107; 1.3.1.76; 4.99.1.4 [C]

Molecular weight: Translated: 64629; Mature: 64629

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSGKLLIVGFGPGSVDHMTKRAREAIEESDVIIGYKTYIELVRDLIVGKEIISTGMTEE
CCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHH
VSRAQAAVKWAERGKTVAVISSGDAGLYGMAGLVYEVLIEKGWTKDSSIEVEVIPGISAI
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHH
HSCAALLGAPIMHDACTISLSDHLTPWELIEKRIDAAAAADFVIALYNPKSGRRTRQIVE
HHHHHHHCCCHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
AQRILLRHRSPETPVGLVKSAYRERQHIVLTDLAHMLDHEIGMLTTVIIGNSTTFVHDGV
HHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEECCCEEEEECCE
MITPRGYQRKYQLAAAVQPLKPHERLRKEAEPWALDQTKPLEDEDEGSTGASGPSEAAVL
EECCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
AGDNQKDRAFTALATAVRPPSVAAATKEAEPTPLAAVLPPLAAPLAKTAKRQPTAAPKRV
ECCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
KETAEDALAALERRKTRKTAAVLLEAAVSPGVANKQFTPEQLIVLAEVVGMDGKMTYTPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCH
HYMKIERLTEDPDGLVARLVDAGLIVMPVGDVLTVKACDFCDGEKKDAIPYAEELARRFG
HHHHHHHHCCCHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHC
GMALPKELKLGINGCGMACYGAVHEDIGLVYRKGKFDLFLGGKTVGRNAHPGQLVAEGIP
CCCCCHHHHCCCCCCCHHHHHHHHHHHHHEEECCCEEEEECCCCCCCCCCCCCCEECCCC
PEEIVAVMAEIIEQYQEQAHPNERFHKFFRRVKQVGRFSHEEAKPAASIEMPACGE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MMSGKLLIVGFGPGSVDHMTKRAREAIEESDVIIGYKTYIELVRDLIVGKEIISTGMTEE
CCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHH
VSRAQAAVKWAERGKTVAVISSGDAGLYGMAGLVYEVLIEKGWTKDSSIEVEVIPGISAI
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHH
HSCAALLGAPIMHDACTISLSDHLTPWELIEKRIDAAAAADFVIALYNPKSGRRTRQIVE
HHHHHHHCCCHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
AQRILLRHRSPETPVGLVKSAYRERQHIVLTDLAHMLDHEIGMLTTVIIGNSTTFVHDGV
HHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEECCCEEEEECCE
MITPRGYQRKYQLAAAVQPLKPHERLRKEAEPWALDQTKPLEDEDEGSTGASGPSEAAVL
EECCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
AGDNQKDRAFTALATAVRPPSVAAATKEAEPTPLAAVLPPLAAPLAKTAKRQPTAAPKRV
ECCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
KETAEDALAALERRKTRKTAAVLLEAAVSPGVANKQFTPEQLIVLAEVVGMDGKMTYTPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCH
HYMKIERLTEDPDGLVARLVDAGLIVMPVGDVLTVKACDFCDGEKKDAIPYAEELARRFG
HHHHHHHHCCCHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHC
GMALPKELKLGINGCGMACYGAVHEDIGLVYRKGKFDLFLGGKTVGRNAHPGQLVAEGIP
CCCCCHHHHCCCCCCCHHHHHHHHHHHHHEEECCCEEEEECCCCCCCCCCCCCCEECCCC
PEEIVAVMAEIIEQYQEQAHPNERFHKFFRRVKQVGRFSHEEAKPAASIEMPACGE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2S-Adenosyl-L-methionine; uroporphyrin III; dihydrosirohydrochlorin; NAD; sirohydrochlorin; Fe (II) [C]

Specific reaction: 2S-Adenosyl-L-methionine + uroporphyrin III = 2S-adenosyl-L-homocysteine + precorrin dihydrosirohydrochlorin + NAD = sirohydrochlorin + NADH sirohydrochlorin + Fe (II) = 2 Proton + siroheme [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]