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The map label for this gene is rsmF [H]

Identifier: 138895319

GI number: 138895319

Start: 1755493

End: 1756866

Strand: Direct

Name: rsmF [H]

Synonym: GTNG_1663

Alternate gene names: 138895319

Gene position: 1755493-1756866 (Clockwise)

Preceding gene: 138895318

Following gene: 138895336

Centisome position: 49.45

GC content: 55.02

Gene sequence:

>1374_bases
TTGCTGTCCTTGAGATTGCCGGAAGCATTTGTCGCGAAAATGAAAAAGCTGCTTCATGAAGAAGCAGACGAATTTTTCGC
CGTCTACGAAAATGAAAAAACGAACGGTTTGCGCGTCAATCCGTTGAAAACTAGCCCGGATTCATGGGCAAAAATGGCGC
CATTTTCCCTCACCCCGGTGCCGTTTTGCCCAACCGGGTTTTATTACGACCGAAGCGAACAGCCAGGAAAACATCCATAC
CATGCAGCCGGGCTGTACTACATTCAAGAACCGAGCGCAATGGCAGTCGCCGAAGCGCTCCGTCCCGCACCCGGGGAGAC
GGTGCTTGATTTGTGCGCCGCTCCGGGCGGGAAAACGACCCAGCTTGGTGCCATGATGGAAAACAAAGGACTGCTTGTCG
CTAACGAAATTCACCCGAAACGAGTTAAAGCGCTCGCGGAAAACGTCGAACGGCTCGGCTTGACCAACACCGTGGTCGTC
AACGAAACGCCGGAAGCGCTCGCGGAGCGGTTCCCTGGCTTTTTTGACAAAATTTTAGTCGATGCCCCGTGCTCGGGTGA
AGGGATGTTTCGCAAAGAGGAAGAGGCGGCTTCATTTTGGAGTCAGGCGTACGTAGAAGAGTGCGCGGCCCGTCAGCGGC
GCATTTTGCAAAGCGCCTATGCGATGCTGAAGGAAGGGGGTATTCTCGTCTACTCGACGTGCACGTTCTCTCCAGAAGAA
AACGAGCAGACGATCGAATGGCTGCTTCAGACGTATGACGACTTGCGGATGGTGCCGATTGAAAAAACCGGCGGCATAGA
GCCCGGGCGGCCTGAGTGGACGAAAACGAACCGGGCTGAACTTGAACATGCCGCACGTCTTTGGCCGCACCGTGTCAAAG
GCGAAGGCCATTTTATCGCCAAACTGCAAAAACAGCGACCGACTGCACCTTGGAACGGGCGGCGGGCAAAGGCGAACGCA
CCGAAAGCCGCGATTCGGTTGTACCGTCAATTCGAACAAGCGACACTGCGGACAGAACGGCACGGAACGGTCGTATCTTT
TGGCTCCCAGTTGGTCATGCTGCCGGAGCAATGCCCCGATTGGTCAGGTTTGAAAGTGGTTCGCGCCGGACTTCATCTTG
GCGAGGTGAAAAAAGAGCGGTTTGAGCCAAACCACGCCCTCGCTTTGGCGCTGCGGACGGATGAAGTGAACCATGTGCTT
GATCTCCCAAGCACAGGCCATGAGATCATCCGATATTGGCATGGAGAGACGCTTCCGACTGGCGGCGACCGTGGCTGGTT
GCTTGTTACCGTCGACGGTTATCCATTCGCTTGGGGGAAAGAAGTGAAAGGAACGGTAAAAAACTTTTACCCGAAAGGGC
TGCGTCTTGTGTGA

Upstream 100 bases:

>100_bases
TTTATCGTCACCACGCCAATATTCGACGCATCTTGAATAAAACGGAGCCAAAAGTGAAATGGCCAAGAAAGCGGAAATAA
CGACAGATGGAGAAAGGAGT

Downstream 100 bases:

>100_bases
GCGACTCCCCTTTCTTTTCCTGAAGTAAGAGGATTCTTTCGAAACGATGTTACAACGAACGGCAAAGACTAGACCGTCTT
GGAAGAAAACAGAGGCTGTC

Product: tRNA and rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MLSLRLPEAFVAKMKKLLHEEADEFFAVYENEKTNGLRVNPLKTSPDSWAKMAPFSLTPVPFCPTGFYYDRSEQPGKHPY
HAAGLYYIQEPSAMAVAEALRPAPGETVLDLCAAPGGKTTQLGAMMENKGLLVANEIHPKRVKALAENVERLGLTNTVVV
NETPEALAERFPGFFDKILVDAPCSGEGMFRKEEEAASFWSQAYVEECAARQRRILQSAYAMLKEGGILVYSTCTFSPEE
NEQTIEWLLQTYDDLRMVPIEKTGGIEPGRPEWTKTNRAELEHAARLWPHRVKGEGHFIAKLQKQRPTAPWNGRRAKANA
PKAAIRLYRQFEQATLRTERHGTVVSFGSQLVMLPEQCPDWSGLKVVRAGLHLGEVKKERFEPNHALALALRTDEVNHVL
DLPSTGHEIIRYWHGETLPTGGDRGWLLVTVDGYPFAWGKEVKGTVKNFYPKGLRLV

Sequences:

>Translated_457_residues
MLSLRLPEAFVAKMKKLLHEEADEFFAVYENEKTNGLRVNPLKTSPDSWAKMAPFSLTPVPFCPTGFYYDRSEQPGKHPY
HAAGLYYIQEPSAMAVAEALRPAPGETVLDLCAAPGGKTTQLGAMMENKGLLVANEIHPKRVKALAENVERLGLTNTVVV
NETPEALAERFPGFFDKILVDAPCSGEGMFRKEEEAASFWSQAYVEECAARQRRILQSAYAMLKEGGILVYSTCTFSPEE
NEQTIEWLLQTYDDLRMVPIEKTGGIEPGRPEWTKTNRAELEHAARLWPHRVKGEGHFIAKLQKQRPTAPWNGRRAKANA
PKAAIRLYRQFEQATLRTERHGTVVSFGSQLVMLPEQCPDWSGLKVVRAGLHLGEVKKERFEPNHALALALRTDEVNHVL
DLPSTGHEIIRYWHGETLPTGGDRGWLLVTVDGYPFAWGKEVKGTVKNFYPKGLRLV
>Mature_457_residues
MLSLRLPEAFVAKMKKLLHEEADEFFAVYENEKTNGLRVNPLKTSPDSWAKMAPFSLTPVPFCPTGFYYDRSEQPGKHPY
HAAGLYYIQEPSAMAVAEALRPAPGETVLDLCAAPGGKTTQLGAMMENKGLLVANEIHPKRVKALAENVERLGLTNTVVV
NETPEALAERFPGFFDKILVDAPCSGEGMFRKEEEAASFWSQAYVEECAARQRRILQSAYAMLKEGGILVYSTCTFSPEE
NEQTIEWLLQTYDDLRMVPIEKTGGIEPGRPEWTKTNRAELEHAARLWPHRVKGEGHFIAKLQKQRPTAPWNGRRAKANA
PKAAIRLYRQFEQATLRTERHGTVVSFGSQLVMLPEQCPDWSGLKVVRAGLHLGEVKKERFEPNHALALALRTDEVNHVL
DLPSTGHEIIRYWHGETLPTGGDRGWLLVTVDGYPFAWGKEVKGTVKNFYPKGLRLV

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=297, Percent_Identity=34.3434343434343, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI76150623, Length=297, Percent_Identity=34.3434343434343, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI32698918, Length=198, Percent_Identity=37.8787878787879, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI39995082, Length=395, Percent_Identity=27.3417721518987, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI301336155, Length=303, Percent_Identity=29.3729372937294, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI11545785, Length=260, Percent_Identity=32.6923076923077, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI270288818, Length=255, Percent_Identity=29.4117647058824, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI8922322, Length=255, Percent_Identity=29.4117647058824, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI23199998, Length=255, Percent_Identity=29.4117647058824, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI270288816, Length=255, Percent_Identity=29.4117647058824, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI40316918, Length=248, Percent_Identity=29.4354838709677, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI87081985, Length=482, Percent_Identity=31.3278008298755, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI2367212, Length=312, Percent_Identity=31.0897435897436, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17536757, Length=294, Percent_Identity=34.0136054421769, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI25143471, Length=177, Percent_Identity=36.1581920903955, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115534021, Length=242, Percent_Identity=30.9917355371901, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71998419, Length=242, Percent_Identity=30.5785123966942, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324268, Length=322, Percent_Identity=34.1614906832298, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319447, Length=276, Percent_Identity=31.8840579710145, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324306, Length=268, Percent_Identity=25.7462686567164, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI22024126, Length=243, Percent_Identity=35.8024691358025, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24668781, Length=237, Percent_Identity=32.0675105485232, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21356513, Length=237, Percent_Identity=32.0675105485232, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21355201, Length=287, Percent_Identity=29.2682926829268, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356579, Length=235, Percent_Identity=27.2340425531915, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19920494, Length=162, Percent_Identity=30.8641975308642, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 51292; Mature: 51292

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLRLPEAFVAKMKKLLHEEADEFFAVYENEKTNGLRVNPLKTSPDSWAKMAPFSLTPV
CCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEECCCCCCCCCHHHCCCCCCCCC
PFCPTGFYYDRSEQPGKHPYHAAGLYYIQEPSAMAVAEALRPAPGETVLDLCAAPGGKTT
CCCCCCCEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCCCCCH
QLGAMMENKGLLVANEIHPKRVKALAENVERLGLTNTVVVNETPEALAERFPGFFDKILV
HHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCHHHHHHC
DAPCSGEGMFRKEEEAASFWSQAYVEECAARQRRILQSAYAMLKEGGILVYSTCTFSPEE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
NEQTIEWLLQTYDDLRMVPIEKTGGIEPGRPEWTKTNRAELEHAARLWPHRVKGEGHFIA
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHCCCCCCEEE
KLQKQRPTAPWNGRRAKANAPKAAIRLYRQFEQATLRTERHGTVVSFGSQLVMLPEQCPD
EEHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEECHHHCCC
WSGLKVVRAGLHLGEVKKERFEPNHALALALRTDEVNHVLDLPSTGHEIIRYWHGETLPT
CCCHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCCCEECCCCCHHHHHHHHCCCCCCC
GGDRGWLLVTVDGYPFAWGKEVKGTVKNFYPKGLRLV
CCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLSLRLPEAFVAKMKKLLHEEADEFFAVYENEKTNGLRVNPLKTSPDSWAKMAPFSLTPV
CCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEECCCCCCCCCHHHCCCCCCCCC
PFCPTGFYYDRSEQPGKHPYHAAGLYYIQEPSAMAVAEALRPAPGETVLDLCAAPGGKTT
CCCCCCCEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCCCCCH
QLGAMMENKGLLVANEIHPKRVKALAENVERLGLTNTVVVNETPEALAERFPGFFDKILV
HHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCHHHHHHC
DAPCSGEGMFRKEEEAASFWSQAYVEECAARQRRILQSAYAMLKEGGILVYSTCTFSPEE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
NEQTIEWLLQTYDDLRMVPIEKTGGIEPGRPEWTKTNRAELEHAARLWPHRVKGEGHFIA
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHCCCCCCEEE
KLQKQRPTAPWNGRRAKANAPKAAIRLYRQFEQATLRTERHGTVVSFGSQLVMLPEQCPD
EEHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEECHHHCCC
WSGLKVVRAGLHLGEVKKERFEPNHALALALRTDEVNHVLDLPSTGHEIIRYWHGETLPT
CCCHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCCCEECCCCCHHHHHHHHCCCCCCC
GGDRGWLLVTVDGYPFAWGKEVKGTVKNFYPKGLRLV
CCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA