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The map label for this gene is topB [H]

Identifier: 138895193

GI number: 138895193

Start: 1613312

End: 1615402

Strand: Reverse

Name: topB [H]

Synonym: GTNG_1531

Alternate gene names: 138895193

Gene position: 1615402-1613312 (Counterclockwise)

Preceding gene: 138895194

Following gene: 138895192

Centisome position: 45.5

GC content: 53.66

Gene sequence:

>2091_bases
ATGAAATCGCTCGTTTTAGCGGAAAAGCCGAGCGTCGCCCGTGACATCGCCCGCGTGCTCGGCTGCAAAAAAACACATAA
ATCATACTTGGAAGGGCCGCGCTACATCGTCACATGGGCGCTTGGGCATTTGGTTGAACTCAAAATGCCCGAAGACTATG
ACCGGAAATATGAAACGTGGCGGCTCGAGGACTTGCCGATCATTCCGAAAAAAATGGGCTTGAAAGTGATCCGGCAAACG
AGCCACCAGTTTCGCGCCATTGAACGTTTAGCCAAACGCCGCGATGTGAAAGATTGCATTATTGCGACCGACGCCGGCCG
CGAGGGGGAGCTGGTCGCGCGTTGGATTTTGCAAAAAATCGACTGGAAGAAGCCGATTTGGCGTTTATGGATTTCATCGC
AAACCGATCAAGCCATTCGCGACGGTTTTCGCCAGTTAAAACCCGGGACGCAGTTTGACCGGCTGTATGAATCAGCCATC
TGCCGCGCCGAAGCGGACTGGCTGATCGGGTTGAACGTGACCCGCGCCTTGACGACGAAATACAACGATCCGCTCTCAGC
CGGGCGTGTGCAAACGCCGACGCTCGCGATGATCATCGAACGCGAACGGGAGATTCAATCGTTCGTTCCCGTTCCGTATT
GGACGATTCACGCCCGCATCGGTTCTGTCACTGCGGTATGGGAACGGCACGGTGGGAAACGGCTGTTTGACAAAGACGAA
GCCAATGAGCTGATGACTCGCCTCAAAGGCCAGCCGGCGCGTGTTACATCGGTCAAGCGGAAGCGGAAAAGTGAACCGGT
CCCGTTGCCGTATGATTTAACCGAGTTGCAGCGGGAGGCGAACAAGCGGTTTGGCTTTTCGGCGAAAAAAACGCTCTCGG
TATTGCAGCGGCTATATGAACAGCACAAGCTCGTTACGTACCCGCGCACGGATTCACGCTATTTGCCAAGCGATATGAAA
GCGACGATGACCGATCGGCTTTTGGCGATGAAGCCAGGCTATGAAGACGTGATCTCCTCCCTGCTCACCAAAAAGAACGT
CAAGGCAGCCAACCGGGTGTTCCAAGATGACAAAGTGACCGATCACCACGCCATCATTCCGACCGATGAACGATTGGACC
TCGCGAAGCTGTCGGTGGATGAACGCAAGCTGTATGACATGATTGCCCGCCGCTTTTTGGCACTGTTTTACCCACCGTAT
GAATACGAAACGGCAACCGTCGTCTTCGAGATCGGCGACGAAACGTTTGTCGCCCGGGAGACGGCGGTCGTGAACGCAGG
GTTTAAAGCCGTTCTTGGCAAAGAAGAAACAGAAGCGAAACCAACGTTGTTACATCTATCGGAAGGCCAGACGCTCGCAT
CGGTACAACTTGAGATGGAACAATCGTTCACCGAACCGCCGTCCCGTTACTCAGAAGCCGATTTGCTTGCACAAATGGAG
AGATATGGACTCGGCACACCGGCAACAAGAGCGGACATCATCGAGCGGCTCGTCGAAACGGAAGTGGTCGAGCGGAAAGA
CGGACGCTTTTATCCGACGAAAAAGGGAAAGCAGTTGATCGAACTGGTCAACGAGGAACTGAAATCGCCGGAGTTGACCG
CCCGCTGGGAGCGTGAGCTCGAGGCGATCGCTCGTGGAAAAGGAAATCCAAAACAATTTTTGGCTAACATCCGCCGACAA
ACGGAGCAGCTCGTCACTGAAATCAAGCAGAGTGAACAGACGTACAAAGCGCCAAACCTCACGAATCTCACCTGTCCGGA
ATGCGGTTCACTCTTAAAAGAGCGGAAAACGAAAGACGGACGGATGCTCGTCTGTTCCAACTTGGAATGCCGCTACCGCC
GGAGACGTGATCCGAAGTTGTCGAATCGCCGTTGCCCGCAATGCCACCGACGCATGGAAATGCATGAAGGAAAGGCCGGG
CTCTATTTCCAGTGCCGCCCATGCAACATTGTAGAAAAAGCGGAAGAAACGAAACGCACATCCGCGAAAGGAACGGAGCG
GGCGTTGCTCAAAAAATACAGCCCGTCCAATGAATCGTTTGGCACAAGCTTAGGAGAGCTGCTCAAACAGGCACTTGAAA
AAAAGGAATAA

Upstream 100 bases:

>100_bases
TTCGCTCAGGGTCAAGCCTAGCGGGTGCGTCTTTTTCTTTCTTCCATTATACTAATAAACAGACCGCACCTAGTGGGAGA
CGACAGAAAGGAGTTTTTTT

Downstream 100 bases:

>100_bases
TCACTGTCAACCGCCAGAAGCTATGACTCTGGCGGTTTCTTTGTATCGCTGAATCTGAAACCTTCTTTCTCTTCACTCCG
TATATATAAGTAAATCTACC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 696; Mature: 696

Protein sequence:

>696_residues
MKSLVLAEKPSVARDIARVLGCKKTHKSYLEGPRYIVTWALGHLVELKMPEDYDRKYETWRLEDLPIIPKKMGLKVIRQT
SHQFRAIERLAKRRDVKDCIIATDAGREGELVARWILQKIDWKKPIWRLWISSQTDQAIRDGFRQLKPGTQFDRLYESAI
CRAEADWLIGLNVTRALTTKYNDPLSAGRVQTPTLAMIIEREREIQSFVPVPYWTIHARIGSVTAVWERHGGKRLFDKDE
ANELMTRLKGQPARVTSVKRKRKSEPVPLPYDLTELQREANKRFGFSAKKTLSVLQRLYEQHKLVTYPRTDSRYLPSDMK
ATMTDRLLAMKPGYEDVISSLLTKKNVKAANRVFQDDKVTDHHAIIPTDERLDLAKLSVDERKLYDMIARRFLALFYPPY
EYETATVVFEIGDETFVARETAVVNAGFKAVLGKEETEAKPTLLHLSEGQTLASVQLEMEQSFTEPPSRYSEADLLAQME
RYGLGTPATRADIIERLVETEVVERKDGRFYPTKKGKQLIELVNEELKSPELTARWERELEAIARGKGNPKQFLANIRRQ
TEQLVTEIKQSEQTYKAPNLTNLTCPECGSLLKERKTKDGRMLVCSNLECRYRRRRDPKLSNRRCPQCHRRMEMHEGKAG
LYFQCRPCNIVEKAEETKRTSAKGTERALLKKYSPSNESFGTSLGELLKQALEKKE

Sequences:

>Translated_696_residues
MKSLVLAEKPSVARDIARVLGCKKTHKSYLEGPRYIVTWALGHLVELKMPEDYDRKYETWRLEDLPIIPKKMGLKVIRQT
SHQFRAIERLAKRRDVKDCIIATDAGREGELVARWILQKIDWKKPIWRLWISSQTDQAIRDGFRQLKPGTQFDRLYESAI
CRAEADWLIGLNVTRALTTKYNDPLSAGRVQTPTLAMIIEREREIQSFVPVPYWTIHARIGSVTAVWERHGGKRLFDKDE
ANELMTRLKGQPARVTSVKRKRKSEPVPLPYDLTELQREANKRFGFSAKKTLSVLQRLYEQHKLVTYPRTDSRYLPSDMK
ATMTDRLLAMKPGYEDVISSLLTKKNVKAANRVFQDDKVTDHHAIIPTDERLDLAKLSVDERKLYDMIARRFLALFYPPY
EYETATVVFEIGDETFVARETAVVNAGFKAVLGKEETEAKPTLLHLSEGQTLASVQLEMEQSFTEPPSRYSEADLLAQME
RYGLGTPATRADIIERLVETEVVERKDGRFYPTKKGKQLIELVNEELKSPELTARWERELEAIARGKGNPKQFLANIRRQ
TEQLVTEIKQSEQTYKAPNLTNLTCPECGSLLKERKTKDGRMLVCSNLECRYRRRRDPKLSNRRCPQCHRRMEMHEGKAG
LYFQCRPCNIVEKAEETKRTSAKGTERALLKKYSPSNESFGTSLGELLKQALEKKE
>Mature_696_residues
MKSLVLAEKPSVARDIARVLGCKKTHKSYLEGPRYIVTWALGHLVELKMPEDYDRKYETWRLEDLPIIPKKMGLKVIRQT
SHQFRAIERLAKRRDVKDCIIATDAGREGELVARWILQKIDWKKPIWRLWISSQTDQAIRDGFRQLKPGTQFDRLYESAI
CRAEADWLIGLNVTRALTTKYNDPLSAGRVQTPTLAMIIEREREIQSFVPVPYWTIHARIGSVTAVWERHGGKRLFDKDE
ANELMTRLKGQPARVTSVKRKRKSEPVPLPYDLTELQREANKRFGFSAKKTLSVLQRLYEQHKLVTYPRTDSRYLPSDMK
ATMTDRLLAMKPGYEDVISSLLTKKNVKAANRVFQDDKVTDHHAIIPTDERLDLAKLSVDERKLYDMIARRFLALFYPPY
EYETATVVFEIGDETFVARETAVVNAGFKAVLGKEETEAKPTLLHLSEGQTLASVQLEMEQSFTEPPSRYSEADLLAQME
RYGLGTPATRADIIERLVETEVVERKDGRFYPTKKGKQLIELVNEELKSPELTARWERELEAIARGKGNPKQFLANIRRQ
TEQLVTEIKQSEQTYKAPNLTNLTCPECGSLLKERKTKDGRMLVCSNLECRYRRRRDPKLSNRRCPQCHRRMEMHEGKAG
LYFQCRPCNIVEKAEETKRTSAKGTERALLKKYSPSNESFGTSLGELLKQALEKKE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=683, Percent_Identity=26.7935578330893, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI10835218, Length=672, Percent_Identity=27.9761904761905, Blast_Score=169, Evalue=9e-42,
Organism=Escherichia coli, GI1788061, Length=615, Percent_Identity=34.3089430894309, Blast_Score=324, Evalue=1e-89,
Organism=Escherichia coli, GI1787529, Length=705, Percent_Identity=28.2269503546099, Blast_Score=199, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI32563869, Length=599, Percent_Identity=27.3789649415693, Blast_Score=162, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17555378, Length=631, Percent_Identity=26.6244057052298, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6323263, Length=651, Percent_Identity=24.2703533026114, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24585251, Length=749, Percent_Identity=25.9012016021362, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24640096, Length=609, Percent_Identity=26.1083743842365, Blast_Score=156, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 80354; Mature: 80354

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLVLAEKPSVARDIARVLGCKKTHKSYLEGPRYIVTWALGHLVELKMPEDYDRKYETW
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCEEEEEHHHHHEEECCCCCCCCCCCCE
RLEDLPIIPKKMGLKVIRQTSHQFRAIERLAKRRDVKDCIIATDAGREGELVARWILQKI
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
DWKKPIWRLWISSQTDQAIRDGFRQLKPGTQFDRLYESAICRAEADWLIGLNVTRALTTK
CCCCCHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHC
YNDPLSAGRVQTPTLAMIIEREREIQSFVPVPYWTIHARIGSVTAVWERHGGKRLFDKDE
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHHHHCCCCCCCCCCH
ANELMTRLKGQPARVTSVKRKRKSEPVPLPYDLTELQREANKRFGFSAKKTLSVLQRLYE
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
QHKLVTYPRTDSRYLPSDMKATMTDRLLAMKPGYEDVISSLLTKKNVKAANRVFQDDKVT
CCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DHHAIIPTDERLDLAKLSVDERKLYDMIARRFLALFYPPYEYETATVVFEIGDETFVARE
CCCEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEHHH
TAVVNAGFKAVLGKEETEAKPTLLHLSEGQTLASVQLEMEQSFTEPPSRYSEADLLAQME
HHHHHCCHHHHCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHH
RYGLGTPATRADIIERLVETEVVERKDGRFYPTKKGKQLIELVNEELKSPELTARWEREL
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
EAIARGKGNPKQFLANIRRQTEQLVTEIKQSEQTYKAPNLTNLTCPECGSLLKERKTKDG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
RMLVCSNLECRYRRRRDPKLSNRRCPQCHRRMEMHEGKAGLYFQCRPCNIVEKAEETKRT
CEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHH
SAKGTERALLKKYSPSNESFGTSLGELLKQALEKKE
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSLVLAEKPSVARDIARVLGCKKTHKSYLEGPRYIVTWALGHLVELKMPEDYDRKYETW
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCEEEEEHHHHHEEECCCCCCCCCCCCE
RLEDLPIIPKKMGLKVIRQTSHQFRAIERLAKRRDVKDCIIATDAGREGELVARWILQKI
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
DWKKPIWRLWISSQTDQAIRDGFRQLKPGTQFDRLYESAICRAEADWLIGLNVTRALTTK
CCCCCHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHC
YNDPLSAGRVQTPTLAMIIEREREIQSFVPVPYWTIHARIGSVTAVWERHGGKRLFDKDE
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHHHHCCCCCCCCCCH
ANELMTRLKGQPARVTSVKRKRKSEPVPLPYDLTELQREANKRFGFSAKKTLSVLQRLYE
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
QHKLVTYPRTDSRYLPSDMKATMTDRLLAMKPGYEDVISSLLTKKNVKAANRVFQDDKVT
CCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DHHAIIPTDERLDLAKLSVDERKLYDMIARRFLALFYPPYEYETATVVFEIGDETFVARE
CCCEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEHHH
TAVVNAGFKAVLGKEETEAKPTLLHLSEGQTLASVQLEMEQSFTEPPSRYSEADLLAQME
HHHHHCCHHHHCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHH
RYGLGTPATRADIIERLVETEVVERKDGRFYPTKKGKQLIELVNEELKSPELTARWEREL
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
EAIARGKGNPKQFLANIRRQTEQLVTEIKQSEQTYKAPNLTNLTCPECGSLLKERKTKDG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
RMLVCSNLECRYRRRRDPKLSNRRCPQCHRRMEMHEGKAGLYFQCRPCNIVEKAEETKRT
CEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHH
SAKGTERALLKKYSPSNESFGTSLGELLKQALEKKE
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA