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The map label for this gene is bioB [H]

Identifier: 138895080

GI number: 138895080

Start: 1509264

End: 1510262

Strand: Direct

Name: bioB [H]

Synonym: GTNG_1418

Alternate gene names: 138895080

Gene position: 1509264-1510262 (Clockwise)

Preceding gene: 138895079

Following gene: 138895081

Centisome position: 42.51

GC content: 52.85

Gene sequence:

>999_bases
ATGCCGAATTGGCTGTCATTAGCCGAACAGGTGATGGAAGGATACGAGCTGGCCGATGAAGAGGCGTTGGCGATACTCGA
CTGTCCGGACGACGAGCTGCTCCTTCTTTTGCAAGGGGCGTACCGAATCCGCTCGGTCTATTACGGCAATAAGGTGAAAT
TGAATATGATCATGAACGCCAAATCCGGCCTTTGTCCGGAAAACTGCGGCTACTGTTCGCAGTCTGCCGTGGCGACGGCG
CCGGTGAAGACGTATAAAATGCTCGATAAAGAAACGCTGCTGCGCGGGGCTGAAGAAGCGTACCGTTTGCAGATAGGGAC
ATATTGCATTGTCGCAAGCGGCCGCGGTCCGAGCGATAAGGAAGTAGACATTGTCGTTTCCGCCGTGAAAGAAATTAAAG
AACGGTTCGGTCTGAAAGTGTGCGCCTGTCTCGGGCTGTTGAAGCCGGAACAAGCAGCGAGGCTGAAAGAAGCGGGCGTG
GATCGCTACAACCATAATATTAATACGTCGAAACAACACCATCCGAACATTACGACGTCTCATACATATGATGACCGGGT
GCGGACGGTGGAAACGGTGAAGGAAGCAGGTCTTTCGCCGTGCTCGGGCGTTATTATCGGCATGAAAGAAACGAAGCAAG
ATATTGTCGATATGGCGCGCAGCTTGCGGGAGCTCGATGCCGATTCGATCCCGGTCAACTTTTTGCATGCGATCGACGGA
ACGCCGCTGGCCGGAACGAATGAATTAAATCCGCGTTATTGCTTGAAAGTGTTGGCGCTGTTCCGCTATATGAACCCGAC
AAAAGAGATACGCATCGCCGGTGGACGGGAAGTGAATTTGCGCTCGCTCCAGCCGCTCGGGCTGTATGCCGCCAACTCCA
TTTTCGTCGGCGATTATTTAACAACCCTCGGGCAGGAAAAGTCCGCCGATTACAAGATGCTTGAGGACCTTGGCTTTGAG
ATCGAGTTTTCTCCAGCGCCGCAAATCGGAGTCGTATAA

Upstream 100 bases:

>100_bases
TGGATGTTGAATTATAGTAAACCAAATTTATAAATAGGTTGACAATTATCTATCTTGTCCGCTACGATAAATGTAATGAT
GTGGAAAGGGGAGAGGGAAA

Downstream 100 bases:

>100_bases
CGGTTGATAAGGTTGGCCTAAAAGGTTGCCCTTCCTTAAGAGGTGCAACATATTGTCTTACGGTATATGATGACGGAAAA
GGGTGCCCCGGTTGTTTTTT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MPNWLSLAEQVMEGYELADEEALAILDCPDDELLLLLQGAYRIRSVYYGNKVKLNMIMNAKSGLCPENCGYCSQSAVATA
PVKTYKMLDKETLLRGAEEAYRLQIGTYCIVASGRGPSDKEVDIVVSAVKEIKERFGLKVCACLGLLKPEQAARLKEAGV
DRYNHNINTSKQHHPNITTSHTYDDRVRTVETVKEAGLSPCSGVIIGMKETKQDIVDMARSLRELDADSIPVNFLHAIDG
TPLAGTNELNPRYCLKVLALFRYMNPTKEIRIAGGREVNLRSLQPLGLYAANSIFVGDYLTTLGQEKSADYKMLEDLGFE
IEFSPAPQIGVV

Sequences:

>Translated_332_residues
MPNWLSLAEQVMEGYELADEEALAILDCPDDELLLLLQGAYRIRSVYYGNKVKLNMIMNAKSGLCPENCGYCSQSAVATA
PVKTYKMLDKETLLRGAEEAYRLQIGTYCIVASGRGPSDKEVDIVVSAVKEIKERFGLKVCACLGLLKPEQAARLKEAGV
DRYNHNINTSKQHHPNITTSHTYDDRVRTVETVKEAGLSPCSGVIIGMKETKQDIVDMARSLRELDADSIPVNFLHAIDG
TPLAGTNELNPRYCLKVLALFRYMNPTKEIRIAGGREVNLRSLQPLGLYAANSIFVGDYLTTLGQEKSADYKMLEDLGFE
IEFSPAPQIGVV
>Mature_331_residues
PNWLSLAEQVMEGYELADEEALAILDCPDDELLLLLQGAYRIRSVYYGNKVKLNMIMNAKSGLCPENCGYCSQSAVATAP
VKTYKMLDKETLLRGAEEAYRLQIGTYCIVASGRGPSDKEVDIVVSAVKEIKERFGLKVCACLGLLKPEQAARLKEAGVD
RYNHNINTSKQHHPNITTSHTYDDRVRTVETVKEAGLSPCSGVIIGMKETKQDIVDMARSLRELDADSIPVNFLHAIDGT
PLAGTNELNPRYCLKVLALFRYMNPTKEIRIAGGREVNLRSLQPLGLYAANSIFVGDYLTTLGQEKSADYKMLEDLGFEI
EFSPAPQIGVV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=291, Percent_Identity=33.3333333333333, Blast_Score=172, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321725, Length=292, Percent_Identity=34.5890410958904, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36702; Mature: 36570

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNWLSLAEQVMEGYELADEEALAILDCPDDELLLLLQGAYRIRSVYYGNKVKLNMIMNA
CCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEECCEEEEEEEECC
KSGLCPENCGYCSQSAVATAPVKTYKMLDKETLLRGAEEAYRLQIGTYCIVASGRGPSDK
CCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCHHHHHEEEECCEEEEECCCCCCCC
EVDIVVSAVKEIKERFGLKVCACLGLLKPEQAARLKEAGVDRYNHNINTSKQHHPNITTS
HHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC
HTYDDRVRTVETVKEAGLSPCSGVIIGMKETKQDIVDMARSLRELDADSIPVNFLHAIDG
CCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
TPLAGTNELNPRYCLKVLALFRYMNPTKEIRIAGGREVNLRSLQPLGLYAANSIFVGDYL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCEEECCCEEEHHHH
TTLGQEKSADYKMLEDLGFEIEFSPAPQIGVV
HHHCCCCCCCHHHHHHCCCEEEECCCCCCCCC
>Mature Secondary Structure 
PNWLSLAEQVMEGYELADEEALAILDCPDDELLLLLQGAYRIRSVYYGNKVKLNMIMNA
CCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEECCEEEEEEEECC
KSGLCPENCGYCSQSAVATAPVKTYKMLDKETLLRGAEEAYRLQIGTYCIVASGRGPSDK
CCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCHHHHHEEEECCEEEEECCCCCCCC
EVDIVVSAVKEIKERFGLKVCACLGLLKPEQAARLKEAGVDRYNHNINTSKQHHPNITTS
HHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC
HTYDDRVRTVETVKEAGLSPCSGVIIGMKETKQDIVDMARSLRELDADSIPVNFLHAIDG
CCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
TPLAGTNELNPRYCLKVLALFRYMNPTKEIRIAGGREVNLRSLQPLGLYAANSIFVGDYL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCEEECCCEEEHHHH
TTLGQEKSADYKMLEDLGFEIEFSPAPQIGVV
HHHCCCCCCCHHHHHHCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA