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The map label for this gene is amtB [C]

Identifier: 138894975

GI number: 138894975

Start: 1400500

End: 1401807

Strand: Direct

Name: amtB [C]

Synonym: GTNG_1313

Alternate gene names: 138894975

Gene position: 1400500-1401807 (Clockwise)

Preceding gene: 138894974

Following gene: 138894976

Centisome position: 39.45

GC content: 51.83

Gene sequence:

>1308_bases
ATGTACATGGGTGAGAAAACATTGATGTTTGGTCTTGATGCTCTTTGGGTGATGCTCGCGGCGGTGCTCGTCATTGGCAT
GCAAGCGGGGTTTGCCTTGCTTGAGGCAGGATCGACACGCATGAAAAACTCCGGGCATGTGGCCGGCAAACAAATTTTAA
GCTTTGCGCTCGCATCTTTAGCGTTTTGGGCATTTGGCTTTGCTATTACGTTTGGAGCAGGAAACGGCTTCATCGGAACG
GAAGGGTGGTTTTTACAAAAAGGGGAAGGGACGTTTGATTCGCTGTCGTGGGCGAATGTGCCATTGGCACTTAAATTTTT
ATTTCCCCTCGGTTTTGCCGGTGTGTCGCTTGCCATCGCCTGGGGCGGCTTTGCAGAACGGGCGAAACTGTCTGTCTATT
TCGTTTTCGGAACGATTTTTACGATCGTTATTTATCCAGTCATCGGCCACTGGGTGTGGGGCGGTGGCTGGCTCGGACAA
ATGGGAATGCAAGACTTCGCTGGTTCAACAGTTGTCCATTTGCAAGGGGCCATCGCGGCGCTTGTAGCCACTGTATTGCT
TGGACCGCGCATCGGCAAGTTCAATAAAGACAAAACACCGAATGTCATTCCTGGGCATAACCAAGTTTATACCGTGATTG
GCGGTTTGATTTTATGGATTGGTTGGTTCGGCTTTAACGCTGGGAGTACGATGGCAGTTGGTGATGGGTTTTTCGGCTAT
GTCGCGCTGACAACGAATTTGGCTGCTGCGGCAGGAGCGATTGCCGCGATCTTGACAGCAAAAGTGTTAGTCGGCAAAGC
GGACATCCCGGCGATGGTCAACGGCGTGTTAGCGGCATTGGTTGCGATTACGGCAGCATGCGCGTTCGTTGAACCGTGGG
CAGCGGTCATTATCGGGGCTGTCGCGGGATCGTTCACGTTCTGGACGTCCGTTTACTTCGAACGTAAAGGGATTGACGAC
CCGATTTACGCTTTTTCCGTCCATGGTATTGCTGGCATCATCGGCACCATTTCAACCGGATTTTTCGCTTCGCCACGCTT
AGTGGAAATCACCGGTGTCGGCAAAGCCGGTTTAGTATACGGTGGTGGATTCGATCAGTTGATTGTACAAACGGTTGGTG
TGGTGGGTGCAGCGATATATGTGGCCGTCGTCTCGTTTGCCATCTTATATGTCATGAAGAAAACGATCGGGCTGCGCGTC
ACAGCGGAGCAAGAAATTTCCGGGCTTGATATTAGCGAACACGGCGCTTACGGCTATCCGGAACAGCTTGATCCGGCCTA
TCAGCCGAAGACAGCCACTCAGCGATAA

Upstream 100 bases:

>100_bases
ATTTATGAAAGCGGATTCTATAAAAATATACGTTGTATTCGTCTTAACCATGTTATATATTATAACATATAAAAGTAACA
ACAAATAAACAAAAAGGGGG

Downstream 100 bases:

>100_bases
AGATGGAGAAGAAAGCGATGTTGGCAGCGGTAACCGACCGGCTGAAAACGGCCGAGTCGGTTACCGATCTTCGTTTCTGC
CATGACGAGTTGGTGCGCGA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MYMGEKTLMFGLDALWVMLAAVLVIGMQAGFALLEAGSTRMKNSGHVAGKQILSFALASLAFWAFGFAITFGAGNGFIGT
EGWFLQKGEGTFDSLSWANVPLALKFLFPLGFAGVSLAIAWGGFAERAKLSVYFVFGTIFTIVIYPVIGHWVWGGGWLGQ
MGMQDFAGSTVVHLQGAIAALVATVLLGPRIGKFNKDKTPNVIPGHNQVYTVIGGLILWIGWFGFNAGSTMAVGDGFFGY
VALTTNLAAAAGAIAAILTAKVLVGKADIPAMVNGVLAALVAITAACAFVEPWAAVIIGAVAGSFTFWTSVYFERKGIDD
PIYAFSVHGIAGIIGTISTGFFASPRLVEITGVGKAGLVYGGGFDQLIVQTVGVVGAAIYVAVVSFAILYVMKKTIGLRV
TAEQEISGLDISEHGAYGYPEQLDPAYQPKTATQR

Sequences:

>Translated_435_residues
MYMGEKTLMFGLDALWVMLAAVLVIGMQAGFALLEAGSTRMKNSGHVAGKQILSFALASLAFWAFGFAITFGAGNGFIGT
EGWFLQKGEGTFDSLSWANVPLALKFLFPLGFAGVSLAIAWGGFAERAKLSVYFVFGTIFTIVIYPVIGHWVWGGGWLGQ
MGMQDFAGSTVVHLQGAIAALVATVLLGPRIGKFNKDKTPNVIPGHNQVYTVIGGLILWIGWFGFNAGSTMAVGDGFFGY
VALTTNLAAAAGAIAAILTAKVLVGKADIPAMVNGVLAALVAITAACAFVEPWAAVIIGAVAGSFTFWTSVYFERKGIDD
PIYAFSVHGIAGIIGTISTGFFASPRLVEITGVGKAGLVYGGGFDQLIVQTVGVVGAAIYVAVVSFAILYVMKKTIGLRV
TAEQEISGLDISEHGAYGYPEQLDPAYQPKTATQR
>Mature_435_residues
MYMGEKTLMFGLDALWVMLAAVLVIGMQAGFALLEAGSTRMKNSGHVAGKQILSFALASLAFWAFGFAITFGAGNGFIGT
EGWFLQKGEGTFDSLSWANVPLALKFLFPLGFAGVSLAIAWGGFAERAKLSVYFVFGTIFTIVIYPVIGHWVWGGGWLGQ
MGMQDFAGSTVVHLQGAIAALVATVLLGPRIGKFNKDKTPNVIPGHNQVYTVIGGLILWIGWFGFNAGSTMAVGDGFFGY
VALTTNLAAAAGAIAAILTAKVLVGKADIPAMVNGVLAALVAITAACAFVEPWAAVIIGAVAGSFTFWTSVYFERKGIDD
PIYAFSVHGIAGIIGTISTGFFASPRLVEITGVGKAGLVYGGGFDQLIVQTVGVVGAAIYVAVVSFAILYVMKKTIGLRV
TAEQEISGLDISEHGAYGYPEQLDPAYQPKTATQR

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=409, Percent_Identity=35.2078239608802, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI32564042, Length=426, Percent_Identity=32.8638497652582, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI133931100, Length=425, Percent_Identity=31.5294117647059, Blast_Score=201, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17550182, Length=432, Percent_Identity=34.0277777777778, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17550180, Length=436, Percent_Identity=32.5688073394495, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6324187, Length=434, Percent_Identity=30.6451612903226, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325396, Length=432, Percent_Identity=29.8611111111111, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321559, Length=413, Percent_Identity=27.1186440677966, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161078314, Length=410, Percent_Identity=34.390243902439, Blast_Score=220, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45620; Mature: 45620

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00107 PROTEIN_KINASE_ATP ; PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMGEKTLMFGLDALWVMLAAVLVIGMQAGFALLEAGSTRMKNSGHVAGKQILSFALASL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
AFWAFGFAITFGAGNGFIGTEGWFLQKGEGTFDSLSWANVPLALKFLFPLGFAGVSLAIA
HHHHHHHHEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEE
WGGFAERAKLSVYFVFGTIFTIVIYPVIGHWVWGGGWLGQMGMQDFAGSTVVHLQGAIAA
ECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHCCCEEEEHHHHHHH
LVATVLLGPRIGKFNKDKTPNVIPGHNQVYTVIGGLILWIGWFGFNAGSTMAVGDGFFGY
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHH
VALTTNLAAAAGAIAAILTAKVLVGKADIPAMVNGVLAALVAITAACAFVEPWAAVIIGA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGSFTFWTSVYFERKGIDDPIYAFSVHGIAGIIGTISTGFFASPRLVEITGVGKAGLVY
HHHHHHHHHHHHHHHCCCCCCHHEEEHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEE
GGGFDQLIVQTVGVVGAAIYVAVVSFAILYVMKKTIGLRVTAEQEISGLDISEHGAYGYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCC
EQLDPAYQPKTATQR
HHCCCCCCCCCCCCC
>Mature Secondary Structure
MYMGEKTLMFGLDALWVMLAAVLVIGMQAGFALLEAGSTRMKNSGHVAGKQILSFALASL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
AFWAFGFAITFGAGNGFIGTEGWFLQKGEGTFDSLSWANVPLALKFLFPLGFAGVSLAIA
HHHHHHHHEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEE
WGGFAERAKLSVYFVFGTIFTIVIYPVIGHWVWGGGWLGQMGMQDFAGSTVVHLQGAIAA
ECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHCCCEEEEHHHHHHH
LVATVLLGPRIGKFNKDKTPNVIPGHNQVYTVIGGLILWIGWFGFNAGSTMAVGDGFFGY
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHH
VALTTNLAAAAGAIAAILTAKVLVGKADIPAMVNGVLAALVAITAACAFVEPWAAVIIGA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGSFTFWTSVYFERKGIDDPIYAFSVHGIAGIIGTISTGFFASPRLVEITGVGKAGLVY
HHHHHHHHHHHHHHHCCCCCCHHEEEHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEE
GGGFDQLIVQTVGVVGAAIYVAVVSFAILYVMKKTIGLRVTAEQEISGLDISEHGAYGYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCC
EQLDPAYQPKTATQR
HHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]