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The map label for this gene is fadA [H]

Identifier: 138894839

GI number: 138894839

Start: 1245690

End: 1246838

Strand: Direct

Name: fadA [H]

Synonym: GTNG_1175

Alternate gene names: 138894839

Gene position: 1245690-1246838 (Clockwise)

Preceding gene: 138894838

Following gene: 138894840

Centisome position: 35.09

GC content: 57.09

Gene sequence:

>1149_bases
ATGCGTGAAGCGGTGATTGTTGAAGCCGTCAGAACACCGGTCGGTAAACGGAACGGCGTTTTTCGCGACGTTCATCCGGT
CCATTTGGCCGCTACGGTGCTTGATGAAGTCGTGCGTCGGGCCGGCATTGAAAAAGGGCAGGTGGAAGATATCGTCATGG
GCTGCGTGACGCCAATCGCCGAACAAGGATACAACATCGGCCGGCTTGCGGCGTTGGAGGCTGGATTTCCGATCGAAGTG
CCAGCCGTGCAAATCAATCGAATGTGCGGCTCAGGACAGCAGGCGATCCATTTTGCTGCACAGGAAATCCGTTCAGGCGA
TATGGATGTCACCATTGCTGCAGGAGTCGAAAGTATGACGAAAGTTCCGATTTTAAGCGATGGCAACGAGCGGACCATTC
CGCCGTCGCTTCATGAAAAGTACGAATTCATCCATCAAGGCGTCTCGGCGGAGCGAATCGCCAAAAAGTACGGCTTAACG
CGCGAACAGCTTGACGCTTACGCCTACGAAAGCCATCAGCGCGCTTTGGCCGCCTTGCGTGAAGGGAAGTTTCGCGCCGA
AATCGTTCCGGTCAAAGGGCTGGACCGCGATGGTCGTGAAATCCTTGTCACCGATGACGAAGGGCCTCGTCCCGATACAT
CGCCGGAAGCGCTCGCCACGCTTAAGCCGGTGTTTCAAGAAGACGGCGTCATTACCGCCGGCAATGCGAGTCAAATGAGC
GATGGGGCGGCCGCCATTTTGCTGATGGAGCAAGAGGTGGCGCGCCGATTCGGGCTGAAACCGAGAGCGCGAATTATCGC
GCAAACGGTCGTCGGCTCCGACCCGACGTATATGCTGGATGGTGTCATCCCCGCGACAAGGCAAGTGCTGAAAAAAGCCG
GGCTCACGATCGAGGACATCGATCTCATTGAAATTAACGAAGCGTTCGCCCCAGTTGTGCTCGCCTGGCAAAAAGAAATC
GGTGCTCCGCTGGAGAAAGTGAATGTCAACGGCGGCGCCATCGCACTCGGCCATCCGCTCGGCGCAACCGGCGTGAAGCT
GATGACATCGCTCGTTCATGAGCTCGAACGGAGGAAGGGGCGCTACGGGCTGCTGACGATTTGCATCGGCCACGGCATGG
CGACAGCAACAATCATCGAGCGTGAGTAA

Upstream 100 bases:

>100_bases
CGCCTTGAAGGGCGGCGTTTAACGAAAGGGATAGTTGTTGTGAGGCGGATAACGGGAAAAGGAGCAATGACGAAGCGAAG
CAGAAGGAGGAAATGGCATC

Downstream 100 bases:

>100_bases
GGCGTGACAATACACATTGAGCTCGCACCATCAGCAGCGGGAGCGGCCAACAAGCCGCCAAGCCCGCTGCTTTTTTGCTT
ATGAAAAACCGTAGGACGGA

Product: beta-ketothiolase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MREAVIVEAVRTPVGKRNGVFRDVHPVHLAATVLDEVVRRAGIEKGQVEDIVMGCVTPIAEQGYNIGRLAALEAGFPIEV
PAVQINRMCGSGQQAIHFAAQEIRSGDMDVTIAAGVESMTKVPILSDGNERTIPPSLHEKYEFIHQGVSAERIAKKYGLT
REQLDAYAYESHQRALAALREGKFRAEIVPVKGLDRDGREILVTDDEGPRPDTSPEALATLKPVFQEDGVITAGNASQMS
DGAAAILLMEQEVARRFGLKPRARIIAQTVVGSDPTYMLDGVIPATRQVLKKAGLTIEDIDLIEINEAFAPVVLAWQKEI
GAPLEKVNVNGGAIALGHPLGATGVKLMTSLVHELERRKGRYGLLTICIGHGMATATIIERE

Sequences:

>Translated_382_residues
MREAVIVEAVRTPVGKRNGVFRDVHPVHLAATVLDEVVRRAGIEKGQVEDIVMGCVTPIAEQGYNIGRLAALEAGFPIEV
PAVQINRMCGSGQQAIHFAAQEIRSGDMDVTIAAGVESMTKVPILSDGNERTIPPSLHEKYEFIHQGVSAERIAKKYGLT
REQLDAYAYESHQRALAALREGKFRAEIVPVKGLDRDGREILVTDDEGPRPDTSPEALATLKPVFQEDGVITAGNASQMS
DGAAAILLMEQEVARRFGLKPRARIIAQTVVGSDPTYMLDGVIPATRQVLKKAGLTIEDIDLIEINEAFAPVVLAWQKEI
GAPLEKVNVNGGAIALGHPLGATGVKLMTSLVHELERRKGRYGLLTICIGHGMATATIIERE
>Mature_382_residues
MREAVIVEAVRTPVGKRNGVFRDVHPVHLAATVLDEVVRRAGIEKGQVEDIVMGCVTPIAEQGYNIGRLAALEAGFPIEV
PAVQINRMCGSGQQAIHFAAQEIRSGDMDVTIAAGVESMTKVPILSDGNERTIPPSLHEKYEFIHQGVSAERIAKKYGLT
REQLDAYAYESHQRALAALREGKFRAEIVPVKGLDRDGREILVTDDEGPRPDTSPEALATLKPVFQEDGVITAGNASQMS
DGAAAILLMEQEVARRFGLKPRARIIAQTVVGSDPTYMLDGVIPATRQVLKKAGLTIEDIDLIEINEAFAPVVLAWQKEI
GAPLEKVNVNGGAIALGHPLGATGVKLMTSLVHELERRKGRYGLLTICIGHGMATATIIERE

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=399, Percent_Identity=41.3533834586466, Blast_Score=275, Evalue=7e-74,
Organism=Homo sapiens, GI167614485, Length=397, Percent_Identity=38.5390428211587, Blast_Score=274, Evalue=8e-74,
Organism=Homo sapiens, GI4501853, Length=390, Percent_Identity=41.7948717948718, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI4557237, Length=393, Percent_Identity=38.9312977099237, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI4504327, Length=428, Percent_Identity=35.0467289719626, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI194353979, Length=385, Percent_Identity=31.6883116883117, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI19923233, Length=323, Percent_Identity=25.6965944272446, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI302344760, Length=323, Percent_Identity=25.6965944272446, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI302344767, Length=312, Percent_Identity=25.9615384615385, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI48994986, Length=387, Percent_Identity=44.9612403100775, Blast_Score=314, Evalue=7e-87,
Organism=Escherichia coli, GI87082165, Length=394, Percent_Identity=41.8781725888325, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=41.75, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1788554, Length=394, Percent_Identity=41.3705583756345, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1788683, Length=424, Percent_Identity=32.5471698113208, Blast_Score=185, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=40.5128205128205, Blast_Score=280, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI17535921, Length=395, Percent_Identity=36.2025316455696, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI25147385, Length=393, Percent_Identity=34.0966921119593, Blast_Score=225, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17551802, Length=431, Percent_Identity=32.9466357308585, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17535917, Length=388, Percent_Identity=29.8969072164948, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6322031, Length=390, Percent_Identity=37.4358974358974, Blast_Score=232, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6325229, Length=397, Percent_Identity=36.272040302267, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=42.0918367346939, Blast_Score=290, Evalue=8e-79,
Organism=Drosophila melanogaster, GI17648125, Length=388, Percent_Identity=38.659793814433, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24640423, Length=393, Percent_Identity=37.6590330788804, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=34.3529411764706, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 41281; Mature: 41281

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREAVIVEAVRTPVGKRNGVFRDVHPVHLAATVLDEVVRRAGIEKGQVEDIVMGCVTPIA
CCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
EQGYNIGRLAALEAGFPIEVPAVQINRMCGSGQQAIHFAAQEIRSGDMDVTIAAGVESMT
HCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHH
KVPILSDGNERTIPPSLHEKYEFIHQGVSAERIAKKYGLTREQLDAYAYESHQRALAALR
CCCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
EGKFRAEIVPVKGLDRDGREILVTDDEGPRPDTSPEALATLKPVFQEDGVITAGNASQMS
CCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCC
DGAAAILLMEQEVARRFGLKPRARIIAQTVVGSDPTYMLDGVIPATRQVLKKAGLTIEDI
CCCEEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCEEECC
DLIEINEAFAPVVLAWQKEIGAPLEKVNVNGGAIALGHPLGATGVKLMTSLVHELERRKG
EEEEECCHHHHHHHHHHHHHCCCHHEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHCCC
RYGLLTICIGHGMATATIIERE
CCEEEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MREAVIVEAVRTPVGKRNGVFRDVHPVHLAATVLDEVVRRAGIEKGQVEDIVMGCVTPIA
CCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
EQGYNIGRLAALEAGFPIEVPAVQINRMCGSGQQAIHFAAQEIRSGDMDVTIAAGVESMT
HCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHH
KVPILSDGNERTIPPSLHEKYEFIHQGVSAERIAKKYGLTREQLDAYAYESHQRALAALR
CCCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
EGKFRAEIVPVKGLDRDGREILVTDDEGPRPDTSPEALATLKPVFQEDGVITAGNASQMS
CCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCC
DGAAAILLMEQEVARRFGLKPRARIIAQTVVGSDPTYMLDGVIPATRQVLKKAGLTIEDI
CCCEEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCEEECC
DLIEINEAFAPVVLAWQKEIGAPLEKVNVNGGAIALGHPLGATGVKLMTSLVHELERRKG
EEEEECCHHHHHHHHHHHHHCCCHHEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHCCC
RYGLLTICIGHGMATATIIERE
CCEEEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]