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The map label for this gene is rlmN [H]

Identifier: 138894695

GI number: 138894695

Start: 1096365

End: 1097459

Strand: Direct

Name: rlmN [H]

Synonym: GTNG_1027

Alternate gene names: 138894695

Gene position: 1096365-1097459 (Clockwise)

Preceding gene: 138894694

Following gene: 138894696

Centisome position: 30.88

GC content: 52.05

Gene sequence:

>1095_bases
ATGACGAATACGGCTGCCCGCCGGTCCACCGGCGAAGCGCAGCTGCTACCGTTGCCGTCGATTTATTCACTGACGCTTGA
TGAATTGAAGGAATGGCTCGTTGCTCACGGTGAAAAACCGTTTCGTGCCACGCAAATTTATGAGTGGCTGTATGGAAAGC
GTGTTACCGATTTTGCTGAGATGACGAACTTGCCCAAACGACTGCGCGAACAGCTCGCCTCGGCGTTTTCGATCACCACG
TTAAAAACGATCGTGAAGCAAACATCGAAAGACGGGACGATCAAATTTTTGTTCGAGCTGCACGATGGTTATTCGATCGA
GACGGTGCTCATGCGCCATAACTACGGCAATTCTGTGTGCGTCACGACACAAGTTGGATGTCGCATCGGTTGCACGTTTT
GCGCTTCGACTCTCGGTGGGCTGAAGCGCCATTTGGAAGCAGGGGAAATCGTCGCTCAAGTCGTGCAAGTGCAAAAGGCA
CTCGATGAAACGGAAGAGCGTGTCAGCAGCATTGTCGTGATGGGCATCGGTGAGCCGTTTGACAACTACGATGCGCTCAT
CAAGTTTTTGCGTATTGTCAACCATTCGAAAGGGCTGAACATCGGCGCTCGCCATATTACCGTTTCTACGAGCGGCATCA
TCCCGAAAATTTATCAATTTGCCGATGAAGGGATGCAAATTAATTTCGCCATTTCGTTGCATGCGCCGACGACCGAGCTG
CGGACGAAGCTCATGCCGATCAATAAGGCGTACCCGTTGCCAAAGCTGATGGAGGCGGTTCGGTATTACATTGAAAAAAC
GGGGCGGCGCGTGACATTTGAATATGGGCTGTTTGGCGGGGTGAATGACCAGCTTGAGCATGCCGAGCAGCTCGCTGAGC
TGCTAAAAGGGTTGAAATGCCATGTGAACCTCATTCCGGTCAACTATGTGCCGGAACGCAACTATGTGCGCACGCCGCGC
AACCAAATTTTCGCCTTTGAACGGGCGTTGAAAAAACATGGAATTAATGTCACCATTCGCCGCGAACACGGACATGACAT
CGATGCCGCCTGCGGACAGCTTCGCGCGAAGGAGCGAAAAGAAGAGACGAGGTGA

Upstream 100 bases:

>100_bases
GCCGTATGTTCAAAAAGGCATGCTGCAATTGCTGCCGCATTATTTTGATTCCGACGGGTTTTTTATCGCCCGGTTACGAA
AGAAGGGGTAGTAAGTGGAA

Downstream 100 bases:

>100_bases
ATCGGATGCAAGCCGTTTTCCGAACCGATATCGGGCAAATTCGCGAGTATAACGAAGACAGTGGCGGCGTATTTGCCAAT
CAAAGCGGCCAATATTTAGC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MTNTAARRSTGEAQLLPLPSIYSLTLDELKEWLVAHGEKPFRATQIYEWLYGKRVTDFAEMTNLPKRLREQLASAFSITT
LKTIVKQTSKDGTIKFLFELHDGYSIETVLMRHNYGNSVCVTTQVGCRIGCTFCASTLGGLKRHLEAGEIVAQVVQVQKA
LDETEERVSSIVVMGIGEPFDNYDALIKFLRIVNHSKGLNIGARHITVSTSGIIPKIYQFADEGMQINFAISLHAPTTEL
RTKLMPINKAYPLPKLMEAVRYYIEKTGRRVTFEYGLFGGVNDQLEHAEQLAELLKGLKCHVNLIPVNYVPERNYVRTPR
NQIFAFERALKKHGINVTIRREHGHDIDAACGQLRAKERKEETR

Sequences:

>Translated_364_residues
MTNTAARRSTGEAQLLPLPSIYSLTLDELKEWLVAHGEKPFRATQIYEWLYGKRVTDFAEMTNLPKRLREQLASAFSITT
LKTIVKQTSKDGTIKFLFELHDGYSIETVLMRHNYGNSVCVTTQVGCRIGCTFCASTLGGLKRHLEAGEIVAQVVQVQKA
LDETEERVSSIVVMGIGEPFDNYDALIKFLRIVNHSKGLNIGARHITVSTSGIIPKIYQFADEGMQINFAISLHAPTTEL
RTKLMPINKAYPLPKLMEAVRYYIEKTGRRVTFEYGLFGGVNDQLEHAEQLAELLKGLKCHVNLIPVNYVPERNYVRTPR
NQIFAFERALKKHGINVTIRREHGHDIDAACGQLRAKERKEETR
>Mature_363_residues
TNTAARRSTGEAQLLPLPSIYSLTLDELKEWLVAHGEKPFRATQIYEWLYGKRVTDFAEMTNLPKRLREQLASAFSITTL
KTIVKQTSKDGTIKFLFELHDGYSIETVLMRHNYGNSVCVTTQVGCRIGCTFCASTLGGLKRHLEAGEIVAQVVQVQKAL
DETEERVSSIVVMGIGEPFDNYDALIKFLRIVNHSKGLNIGARHITVSTSGIIPKIYQFADEGMQINFAISLHAPTTELR
TKLMPINKAYPLPKLMEAVRYYIEKTGRRVTFEYGLFGGVNDQLEHAEQLAELLKGLKCHVNLIPVNYVPERNYVRTPRN
QIFAFERALKKHGINVTIRREHGHDIDAACGQLRAKERKEETR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=342, Percent_Identity=38.3040935672515, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41193; Mature: 41062

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTAARRSTGEAQLLPLPSIYSLTLDELKEWLVAHGEKPFRATQIYEWLYGKRVTDFAE
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHH
MTNLPKRLREQLASAFSITTLKTIVKQTSKDGTIKFLFELHDGYSIETVLMRHNYGNSVC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEECCCCEEE
VTTQVGCRIGCTFCASTLGGLKRHLEAGEIVAQVVQVQKALDETEERVSSIVVMGIGEPF
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
DNYDALIKFLRIVNHSKGLNIGARHITVSTSGIIPKIYQFADEGMQINFAISLHAPTTEL
CCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEEEECCCHHHH
RTKLMPINKAYPLPKLMEAVRYYIEKTGRRVTFEYGLFGGVNDQLEHAEQLAELLKGLKC
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEE
HVNLIPVNYVPERNYVRTPRNQIFAFERALKKHGINVTIRREHGHDIDAACGQLRAKERK
EEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
EETR
HCCC
>Mature Secondary Structure 
TNTAARRSTGEAQLLPLPSIYSLTLDELKEWLVAHGEKPFRATQIYEWLYGKRVTDFAE
CCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHH
MTNLPKRLREQLASAFSITTLKTIVKQTSKDGTIKFLFELHDGYSIETVLMRHNYGNSVC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEECCCCEEE
VTTQVGCRIGCTFCASTLGGLKRHLEAGEIVAQVVQVQKALDETEERVSSIVVMGIGEPF
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
DNYDALIKFLRIVNHSKGLNIGARHITVSTSGIIPKIYQFADEGMQINFAISLHAPTTEL
CCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEEEECCCHHHH
RTKLMPINKAYPLPKLMEAVRYYIEKTGRRVTFEYGLFGGVNDQLEHAEQLAELLKGLKC
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEE
HVNLIPVNYVPERNYVRTPRNQIFAFERALKKHGINVTIRREHGHDIDAACGQLRAKERK
EEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
EETR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA