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The map label for this gene is odhB [H]

Identifier: 138894556

GI number: 138894556

Start: 962218

End: 963537

Strand: Direct

Name: odhB [H]

Synonym: GTNG_0886

Alternate gene names: 138894556

Gene position: 962218-963537 (Clockwise)

Preceding gene: 138894555

Following gene: 138894557

Centisome position: 27.1

GC content: 52.65

Gene sequence:

>1320_bases
ATGATGACTAACGGAATGTTTGAGGAGGGCATCAACGTGGCTGAGATCAAAGTCCCAGAACTAGCAGAGTCCATCACCGA
AGGTACGATCGCTCAATGGCTGAAAAAGCCGGGCGACTATGTGGAAAAAGGTGAATCGGTTTGTGAGTTAGAAACAGATA
AAGTGAACGTCGAAATTATGGCCGAAGAGTCGGGAGTTTTACAGCAATTGTTGGCGAACGAAGGTGACACGGTCGCTGTC
GGCCAAGCGATCGCCATTATCGGCGAGGGGGCGGCTTCGGCACCGACTGCTGCTCCGCAGGCTGCGCAGCCAACTGACGA
AACACCAACCGTGCCGGCTGACCGCGCCGAGCAACAAGCACCGCAACCGGTCGCTGTCGCCCAAGCACCGGGCCAACGTC
CAGTCGCTTCGCCGGCCGCTCGAAAAATGGCGCGTGAGAAAGGGATTGATTTGACGCAAGTGCCGACCGTTGATCCGCTT
GGACGCGTCCGCAAGCAAGACGTGGCTTCATTTGCCGCTCAACCAGCAGCTGGTTCACAAGCAGCGCCGCAACCGGCTCC
AACGGCTGCGCCGGTTTCTGTGCCAACACAGGCTTCTGCGCCGTCAGCGGAAAGCGGCAAGCTGGTCATCCGTGAAAAAA
TGTCGCGCCGCCGGCAAACGATCGCCAAGCGATTGCTTGAAGTTTCGCAATCGACTGCGATGCTGACGACGTTCAATGAA
ATCGACATGTCAGCCGTTATGGAATTGCGCAAGCGGAAAAAAGACAAGTTTTTAGAAGAGCATGACGTCCGCCTTGGCTT
TATGTCGTTTTTCGTCAAAGCGACGGTGGCAGCGCTGAAAAAATATCCATACGTCAACGCGGAAATTCAAGGCGATGAAA
TTTTGCTGAAAAAATATTACGACATCGGCGTGGCTGTTTCGACCGATGAAGGGCTTGTTGTCCCAGTTGTGCGTGACTGT
GACCGGAAAAACTTCGCTGAAATTGAGCGAGACATCGCTGAATTAGCGGCGAAAGCGCGCAGCAACAAACTGTCGCTTGC
CGATTTGCAAGGCGGTACGTTTACAATCACCAACGGTGGTGTATTCGGCTCGCTTCTTTCGACTCCGCTCTTGAACGGGC
CGCAAGTTGGTATTTTAGGGATGCATTCGATCAAACTGCGTCCGGTAGCTATTGATGAGGAGCGGATTGAAAACCGGCCG
ATGATGTATGTCGCTCTGTCGTACGATCACCGCATCATTGATGGCAAAGAAGCAGTTGGGTTCTTAAAAACAGTGAAAGA
CTTGATCGAAAATCCAGAAGATTTATTGCTTGAAAGTTAA

Upstream 100 bases:

>100_bases
ATATCGGGCGACGTCGGCGAGCGAGCCCGGCAGAGGGCGATCCGGTCGTCCATCGGAAAGAACAGGAGCGCATTATCCGC
TGTGCATTAACGAAACATGA

Downstream 100 bases:

>100_bases
TGCGGAAAAAGGAGCCTGCAGCATGCAGGCTCCACGTTTTTTATCGTCTAAATGGCACGTTGTACCGAATGAACTTTCCT
GTTTTACGGAAAACTACCGT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MMTNGMFEEGINVAEIKVPELAESITEGTIAQWLKKPGDYVEKGESVCELETDKVNVEIMAEESGVLQQLLANEGDTVAV
GQAIAIIGEGAASAPTAAPQAAQPTDETPTVPADRAEQQAPQPVAVAQAPGQRPVASPAARKMAREKGIDLTQVPTVDPL
GRVRKQDVASFAAQPAAGSQAAPQPAPTAAPVSVPTQASAPSAESGKLVIREKMSRRRQTIAKRLLEVSQSTAMLTTFNE
IDMSAVMELRKRKKDKFLEEHDVRLGFMSFFVKATVAALKKYPYVNAEIQGDEILLKKYYDIGVAVSTDEGLVVPVVRDC
DRKNFAEIERDIAELAAKARSNKLSLADLQGGTFTITNGGVFGSLLSTPLLNGPQVGILGMHSIKLRPVAIDEERIENRP
MMYVALSYDHRIIDGKEAVGFLKTVKDLIENPEDLLLES

Sequences:

>Translated_439_residues
MMTNGMFEEGINVAEIKVPELAESITEGTIAQWLKKPGDYVEKGESVCELETDKVNVEIMAEESGVLQQLLANEGDTVAV
GQAIAIIGEGAASAPTAAPQAAQPTDETPTVPADRAEQQAPQPVAVAQAPGQRPVASPAARKMAREKGIDLTQVPTVDPL
GRVRKQDVASFAAQPAAGSQAAPQPAPTAAPVSVPTQASAPSAESGKLVIREKMSRRRQTIAKRLLEVSQSTAMLTTFNE
IDMSAVMELRKRKKDKFLEEHDVRLGFMSFFVKATVAALKKYPYVNAEIQGDEILLKKYYDIGVAVSTDEGLVVPVVRDC
DRKNFAEIERDIAELAAKARSNKLSLADLQGGTFTITNGGVFGSLLSTPLLNGPQVGILGMHSIKLRPVAIDEERIENRP
MMYVALSYDHRIIDGKEAVGFLKTVKDLIENPEDLLLES
>Mature_439_residues
MMTNGMFEEGINVAEIKVPELAESITEGTIAQWLKKPGDYVEKGESVCELETDKVNVEIMAEESGVLQQLLANEGDTVAV
GQAIAIIGEGAASAPTAAPQAAQPTDETPTVPADRAEQQAPQPVAVAQAPGQRPVASPAARKMAREKGIDLTQVPTVDPL
GRVRKQDVASFAAQPAAGSQAAPQPAPTAAPVSVPTQASAPSAESGKLVIREKMSRRRQTIAKRLLEVSQSTAMLTTFNE
IDMSAVMELRKRKKDKFLEEHDVRLGFMSFFVKATVAALKKYPYVNAEIQGDEILLKKYYDIGVAVSTDEGLVVPVVRDC
DRKNFAEIERDIAELAAKARSNKLSLADLQGGTFTITNGGVFGSLLSTPLLNGPQVGILGMHSIKLRPVAIDEERIENRP
MMYVALSYDHRIIDGKEAVGFLKTVKDLIENPEDLLLES

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=58.2608695652174, Blast_Score=272, Evalue=3e-73,
Organism=Homo sapiens, GI31711992, Length=442, Percent_Identity=32.3529411764706, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI110671329, Length=435, Percent_Identity=28.5057471264368, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI203098816, Length=456, Percent_Identity=30.0438596491228, Blast_Score=179, Evalue=6e-45,
Organism=Homo sapiens, GI203098753, Length=456, Percent_Identity=30.0438596491228, Blast_Score=179, Evalue=7e-45,
Organism=Homo sapiens, GI260898739, Length=163, Percent_Identity=36.8098159509202, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786946, Length=424, Percent_Identity=45.5188679245283, Blast_Score=345, Evalue=4e-96,
Organism=Escherichia coli, GI1786305, Length=434, Percent_Identity=31.5668202764977, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=435, Percent_Identity=38.8505747126437, Blast_Score=282, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=30.8035714285714, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=430, Percent_Identity=26.7441860465116, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=226, Percent_Identity=32.3008849557522, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=422, Percent_Identity=39.8104265402844, Blast_Score=302, Evalue=6e-83,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=30.1762114537445, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24645909, Length=242, Percent_Identity=50.4132231404959, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=29.3706293706294, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI20129315, Length=225, Percent_Identity=34.6666666666667, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=34.061135371179, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 47284; Mature: 47284

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTNGMFEEGINVAEIKVPELAESITEGTIAQWLKKPGDYVEKGESVCELETDKVNVEIM
CCCCCCHHHCCCEEEEECHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCEEEEEEE
AEESGVLQQLLANEGDTVAVGQAIAIIGEGAASAPTAAPQAAQPTDETPTVPADRAEQQA
ECCHHHHHHHHHCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
PQPVAVAQAPGQRPVASPAARKMAREKGIDLTQVPTVDPLGRVRKQDVASFAAQPAAGSQ
CCCEEEECCCCCCCCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCC
AAPQPAPTAAPVSVPTQASAPSAESGKLVIREKMSRRRQTIAKRLLEVSQSTAMLTTFNE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
IDMSAVMELRKRKKDKFLEEHDVRLGFMSFFVKATVAALKKYPYVNAEIQGDEILLKKYY
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEHHH
DIGVAVSTDEGLVVPVVRDCDRKNFAEIERDIAELAAKARSNKLSLADLQGGTFTITNGG
CCCEEEECCCCEEEHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCC
VFGSLLSTPLLNGPQVGILGMHSIKLRPVAIDEERIENRPMMYVALSYDHRIIDGKEAVG
HHHHHHHCCCCCCCCEEEEEEEEEEEEEEECCHHHHCCCCEEEEEEECCCEEECCHHHHH
FLKTVKDLIENPEDLLLES
HHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MMTNGMFEEGINVAEIKVPELAESITEGTIAQWLKKPGDYVEKGESVCELETDKVNVEIM
CCCCCCHHHCCCEEEEECHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCEEEEEEE
AEESGVLQQLLANEGDTVAVGQAIAIIGEGAASAPTAAPQAAQPTDETPTVPADRAEQQA
ECCHHHHHHHHHCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
PQPVAVAQAPGQRPVASPAARKMAREKGIDLTQVPTVDPLGRVRKQDVASFAAQPAAGSQ
CCCEEEECCCCCCCCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCC
AAPQPAPTAAPVSVPTQASAPSAESGKLVIREKMSRRRQTIAKRLLEVSQSTAMLTTFNE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
IDMSAVMELRKRKKDKFLEEHDVRLGFMSFFVKATVAALKKYPYVNAEIQGDEILLKKYY
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEHHH
DIGVAVSTDEGLVVPVVRDCDRKNFAEIERDIAELAAKARSNKLSLADLQGGTFTITNGG
CCCEEEECCCCEEEHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCC
VFGSLLSTPLLNGPQVGILGMHSIKLRPVAIDEERIENRPMMYVALSYDHRIIDGKEAVG
HHHHHHHCCCCCCCCEEEEEEEEEEEEEEECCHHHHCCCCEEEEEEECCCEEECCHHHHH
FLKTVKDLIENPEDLLLES
HHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2500417; 9384377 [H]