Click here to switch to the map view.

The map label for this gene is metB [H]

Identifier: 138894417

GI number: 138894417

Start: 813423

End: 814526

Strand: Direct

Name: metB [H]

Synonym: GTNG_0745

Alternate gene names: 138894417

Gene position: 813423-814526 (Clockwise)

Preceding gene: 138894413

Following gene: 138894418

Centisome position: 22.91

GC content: 52.9

Gene sequence:

>1104_bases
ATGAAGAAACTGGAGACATTGTTGGCGCAAATTGGCAATCGGAGCGAAACGGTGACGGGGACGGTCAACCCGCCGGTTTA
TTTTTCCACCGCCTACCGCCATGCCGGCATTGGAGAGTCCACCGGGTTTGACTACATCCGCACCGGCAATCCGACGCGCA
AAATCGTCGAAGAGGCGATCGCGAGGCTTGAAGGTGGTGACCAAGGCTATGCGTTCAGTTCCGGCATGGCCGCCATTCAG
ACAGTGCTCGCCTTGTTTGAGAGCGGAGACGAGTTTCTCGTATCGGCCGACCTTTACGGCGGCACGTACCGACTGTTTGA
GCGCGGCTGGCGTAAATACGGCCTCGGATTTCATTACGTTGATTTTGCTGATTTGGATGCCGTGGAAGAAAAAATCACGG
AACGGACAAAAGCAATCTTTTTGGAAACTCCAACAAACCCGCTTATGCATGAGACCGATATCCGCGCCGTCTCAGAGCTC
GCTAGGCGGTATGGGCTTTTACTGATCGTTGACAACACGTTTTATACGCCAGTGCTTCAACGCCCGATCGAACAAGGGGC
TGATATTGTCATCCATAGCGCCACGAAATATTTAGGCGGCCATAACGACGTCTTAGCTGGTCTAGTTGTTGCTAAGGGCG
AAGAGCTCTGTCAGCGCTTGGCGGAGTACCATAACGCCATCGGCGCCGTTTTGTCGCCGTTTGATTCATGGCTGTTGATT
CGCGGGATGAAAACGTTGGCGCTGCGGATGCGCCAGCATGAAGAAAACGCCAAGCGCATCTGCTCCTTTTTGCGTGAACA
CGAAGAGGTCACTGATGTGCTATACCCGGGAAGAGGGGGAATGTTGTCGTTCCGGATTGCCGATGAAAAATGGGTGAACG
GTTTTTTGCGATGTTTGCGTCTCATTACGTTCGCGGAAAGCCTAGGCGGGGTCGAAAGCTTTATCACGTATCCGGCGACG
CAGACGCACGCTGATATTCCGGAAGAAATCCGCATCCAAAACGGTGTTTGCAACCGTCTACTCCGTTTCTCGGTCGGTAT
CGAGCATGCCGACGATTTGATCGCTGATTTAGCGCAGGCATTGAAAAACATGAAAGAGGTGTGA

Upstream 100 bases:

>100_bases
GATCCAAAAACGAAGTGCTTGGCTGCCGAGACAAGCAGCGGTACGCTTCAGACACCCTATCCGTCGGAAGGGAATTTTTC
GGCAGAAGGAGAGAGATGAG

Downstream 100 bases:

>100_bases
GCGATGGAACGGGAATGGTCGTTTTCGACGAAATTGCTCCATAACAAATGGAAAGTCGATCGACATACAGGGGCGGTGAG
CGTGCCGATCCAGCATGCAT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-acetylhomoserine (thiol)-lyase; O-acetylhomoserine sulfhydrylase; OAH sulfhydrylase [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKKLETLLAQIGNRSETVTGTVNPPVYFSTAYRHAGIGESTGFDYIRTGNPTRKIVEEAIARLEGGDQGYAFSSGMAAIQ
TVLALFESGDEFLVSADLYGGTYRLFERGWRKYGLGFHYVDFADLDAVEEKITERTKAIFLETPTNPLMHETDIRAVSEL
ARRYGLLLIVDNTFYTPVLQRPIEQGADIVIHSATKYLGGHNDVLAGLVVAKGEELCQRLAEYHNAIGAVLSPFDSWLLI
RGMKTLALRMRQHEENAKRICSFLREHEEVTDVLYPGRGGMLSFRIADEKWVNGFLRCLRLITFAESLGGVESFITYPAT
QTHADIPEEIRIQNGVCNRLLRFSVGIEHADDLIADLAQALKNMKEV

Sequences:

>Translated_367_residues
MKKLETLLAQIGNRSETVTGTVNPPVYFSTAYRHAGIGESTGFDYIRTGNPTRKIVEEAIARLEGGDQGYAFSSGMAAIQ
TVLALFESGDEFLVSADLYGGTYRLFERGWRKYGLGFHYVDFADLDAVEEKITERTKAIFLETPTNPLMHETDIRAVSEL
ARRYGLLLIVDNTFYTPVLQRPIEQGADIVIHSATKYLGGHNDVLAGLVVAKGEELCQRLAEYHNAIGAVLSPFDSWLLI
RGMKTLALRMRQHEENAKRICSFLREHEEVTDVLYPGRGGMLSFRIADEKWVNGFLRCLRLITFAESLGGVESFITYPAT
QTHADIPEEIRIQNGVCNRLLRFSVGIEHADDLIADLAQALKNMKEV
>Mature_367_residues
MKKLETLLAQIGNRSETVTGTVNPPVYFSTAYRHAGIGESTGFDYIRTGNPTRKIVEEAIARLEGGDQGYAFSSGMAAIQ
TVLALFESGDEFLVSADLYGGTYRLFERGWRKYGLGFHYVDFADLDAVEEKITERTKAIFLETPTNPLMHETDIRAVSEL
ARRYGLLLIVDNTFYTPVLQRPIEQGADIVIHSATKYLGGHNDVLAGLVVAKGEELCQRLAEYHNAIGAVLSPFDSWLLI
RGMKTLALRMRQHEENAKRICSFLREHEEVTDVLYPGRGGMLSFRIADEKWVNGFLRCLRLITFAESLGGVESFITYPAT
QTHADIPEEIRIQNGVCNRLLRFSVGIEHADDLIADLAQALKNMKEV

Specific function: Catalyzes the transformation of O-succinylhomoserine into cystathionine, as well as O-acetylhomoserine and sulfide into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=372, Percent_Identity=40.3225806451613, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI299473759, Length=372, Percent_Identity=36.8279569892473, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI25453487, Length=371, Percent_Identity=35.8490566037736, Blast_Score=208, Evalue=6e-54,
Organism=Escherichia coli, GI1790375, Length=379, Percent_Identity=40.3693931398417, Blast_Score=280, Evalue=8e-77,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=29.0076335877863, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17560056, Length=361, Percent_Identity=43.213296398892, Blast_Score=283, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI17538055, Length=361, Percent_Identity=42.382271468144, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=36.1185983827493, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319307, Length=370, Percent_Identity=41.0810810810811, Blast_Score=276, Evalue=5e-75,
Organism=Saccharomyces cerevisiae, GI6321254, Length=404, Percent_Identity=30.6930693069307, Blast_Score=179, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=412, Percent_Identity=32.0388349514563, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321904, Length=397, Percent_Identity=29.4710327455919, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321170, Length=277, Percent_Identity=32.8519855595668, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19922554, Length=363, Percent_Identity=41.0468319559229, Blast_Score=286, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48; =2.5.1.49 [H]

Molecular weight: Translated: 41028; Mature: 41028

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLETLLAQIGNRSETVTGTVNPPVYFSTAYRHAGIGESTGFDYIRTGNPTRKIVEEAI
CCHHHHHHHHHCCCCCEEEECCCCCEEEEHHHHHCCCCCCCCCCEEECCCHHHHHHHHHH
ARLEGGDQGYAFSSGMAAIQTVLALFESGDEFLVSADLYGGTYRLFERGWRKYGLGFHYV
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEE
DFADLDAVEEKITERTKAIFLETPTNPLMHETDIRAVSELARRYGLLLIVDNTFYTPVLQ
CHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHH
RPIEQGADIVIHSATKYLGGHNDVLAGLVVAKGEELCQRLAEYHNAIGAVLSPFDSWLLI
HHHHCCCCEEEECHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGMKTLALRMRQHEENAKRICSFLREHEEVTDVLYPGRGGMLSFRIADEKWVNGFLRCLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHH
LITFAESLGGVESFITYPATQTHADIPEEIRIQNGVCNRLLRFSVGIEHADDLIADLAQA
HHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LKNMKEV
HHHHHCC
>Mature Secondary Structure
MKKLETLLAQIGNRSETVTGTVNPPVYFSTAYRHAGIGESTGFDYIRTGNPTRKIVEEAI
CCHHHHHHHHHCCCCCEEEECCCCCEEEEHHHHHCCCCCCCCCCEEECCCHHHHHHHHHH
ARLEGGDQGYAFSSGMAAIQTVLALFESGDEFLVSADLYGGTYRLFERGWRKYGLGFHYV
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEE
DFADLDAVEEKITERTKAIFLETPTNPLMHETDIRAVSELARRYGLLLIVDNTFYTPVLQ
CHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHH
RPIEQGADIVIHSATKYLGGHNDVLAGLVVAKGEELCQRLAEYHNAIGAVLSPFDSWLLI
HHHHCCCCEEEECHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGMKTLALRMRQHEENAKRICSFLREHEEVTDVLYPGRGGMLSFRIADEKWVNGFLRCLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHH
LITFAESLGGVESFITYPATQTHADIPEEIRIQNGVCNRLLRFSVGIEHADDLIADLAQA
HHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LKNMKEV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]