Click here to switch to the map view.

The map label for this gene is ytcB [H]

Identifier: 138894393

GI number: 138894393

Start: 798931

End: 799887

Strand: Reverse

Name: ytcB [H]

Synonym: GTNG_0721

Alternate gene names: 138894393

Gene position: 799887-798931 (Counterclockwise)

Preceding gene: 138894394

Following gene: 138894392

Centisome position: 22.53

GC content: 52.56

Gene sequence:

>957_bases
ATGAACATTCTCGTCACTGGAGCGGCCGGATTTATCGGCTCTCATTTATGTGAAAAACTGCTCGAAAACGATCAACACCA
AGTCATCGGCGTCGACGGCTTTATCGGGCCAACACCGGCGCCGCTGAAGATGAAAAACATCGCCCATTTGCAATCCCATC
CGCGGTTTACGTTGGTTGAGACGGATTTGCTGACAGCCGACTTGCCAGCATTGTTAAAAGATGTGGAAGCCGTATACCAT
TTAGCCGGTATGCCCGGCGTGCGAACAAGCTGGGGAACGGACTTTGCCGACTATGCAGTGCACAACATTTCCGTTACACA
ACGGCTGTTGGAAGCGTGCAAAGACTTGCCCCTCAAACGGTTCATCTATGCTTCAACCTCGTCCGTTTACGGTGAACGGA
GTGGTCCGCTGTCGGAAACACTCGAACCAGTTCCGCTCTCTCCATACGGCATTACAAAGCTCACAGGCGAGCATTTATGC
CGCGTCTATTTCCGCGAGTTTGCCGTACCAGTTGTCATTTTGCGCTATTTTACCGTTTACGGGCCACGCCAACGCCCCGA
TATGTCGTTCCACCGCTTTATTCGCCAACTGCTCGCTGGTCAGCCGCTCACCGTATTCGGCGACGGAACGCAATCGCGCG
ATTTCACCTATATTTCCGATTGCATCGACGGGACGGCAGCCGCGCTCGAACGAGATCATGTAATCGGGGAAACGATCAAC
ATCGGCGGGAAAGAACGTGCATCCGTGAATGATGTGATTCACTTGCTTGAAGTGTTGACCGGAAGAAAGGCGACGATCCA
CTATACGTCCGCCGCCCGCGGGGAGCCGAAACAGACATGGGCCGACTTGACAAAAGCAGAACAGCTGCTCGCATACGAAC
CGACTATCACGCTCATAGATGGATTGCAAAAAGAAATCGAATACATCCGTTCGTTATACAAGGGTGAGCAGCCATGA

Upstream 100 bases:

>100_bases
AAGCGGCAATGAACGGGACACTCGTCTTTGACGGACGAAATTGCTTAGATCGCCGCGCTGTCGAACAAAGCGGCTTGCGC
TACGTGGGGGTGGGACGTCC

Downstream 100 bases:

>100_bases
ACATCGCCTACGTTTGCACGGAAAAACTGCCTTCACCAGCAATAAAGGGCGGTGCGATTCAAGTCATGATTGACGGAATC
GCACCGTTCATTGCCCGCTC

Product: nucleotide sugar epimerase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNILVTGAAGFIGSHLCEKLLENDQHQVIGVDGFIGPTPAPLKMKNIAHLQSHPRFTLVETDLLTADLPALLKDVEAVYH
LAGMPGVRTSWGTDFADYAVHNISVTQRLLEACKDLPLKRFIYASTSSVYGERSGPLSETLEPVPLSPYGITKLTGEHLC
RVYFREFAVPVVILRYFTVYGPRQRPDMSFHRFIRQLLAGQPLTVFGDGTQSRDFTYISDCIDGTAAALERDHVIGETIN
IGGKERASVNDVIHLLEVLTGRKATIHYTSAARGEPKQTWADLTKAEQLLAYEPTITLIDGLQKEIEYIRSLYKGEQP

Sequences:

>Translated_318_residues
MNILVTGAAGFIGSHLCEKLLENDQHQVIGVDGFIGPTPAPLKMKNIAHLQSHPRFTLVETDLLTADLPALLKDVEAVYH
LAGMPGVRTSWGTDFADYAVHNISVTQRLLEACKDLPLKRFIYASTSSVYGERSGPLSETLEPVPLSPYGITKLTGEHLC
RVYFREFAVPVVILRYFTVYGPRQRPDMSFHRFIRQLLAGQPLTVFGDGTQSRDFTYISDCIDGTAAALERDHVIGETIN
IGGKERASVNDVIHLLEVLTGRKATIHYTSAARGEPKQTWADLTKAEQLLAYEPTITLIDGLQKEIEYIRSLYKGEQP
>Mature_318_residues
MNILVTGAAGFIGSHLCEKLLENDQHQVIGVDGFIGPTPAPLKMKNIAHLQSHPRFTLVETDLLTADLPALLKDVEAVYH
LAGMPGVRTSWGTDFADYAVHNISVTQRLLEACKDLPLKRFIYASTSSVYGERSGPLSETLEPVPLSPYGITKLTGEHLC
RVYFREFAVPVVILRYFTVYGPRQRPDMSFHRFIRQLLAGQPLTVFGDGTQSRDFTYISDCIDGTAAALERDHVIGETIN
IGGKERASVNDVIHLLEVLTGRKATIHYTSAARGEPKQTWADLTKAEQLLAYEPTITLIDGLQKEIEYIRSLYKGEQP

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=317, Percent_Identity=32.4921135646688, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI7657641, Length=319, Percent_Identity=26.6457680250784, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1786974, Length=324, Percent_Identity=31.1728395061728, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=27.9538904899135, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI48994969, Length=339, Percent_Identity=29.4985250737463, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790049, Length=239, Percent_Identity=30.5439330543933, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=319, Percent_Identity=29.4670846394984, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17568069, Length=333, Percent_Identity=29.1291291291291, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI115532424, Length=328, Percent_Identity=26.5243902439024, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=326, Percent_Identity=27.3006134969325, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=328, Percent_Identity=27.7439024390244, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319493, Length=337, Percent_Identity=25.2225519287834, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356223, Length=317, Percent_Identity=30.9148264984227, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 35296; Mature: 35296

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVTGAAGFIGSHLCEKLLENDQHQVIGVDGFIGPTPAPLKMKNIAHLQSHPRFTLVE
CCEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEE
TDLLTADLPALLKDVEAVYHLAGMPGVRTSWGTDFADYAVHNISVTQRLLEACKDLPLKR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
FIYASTSSVYGERSGPLSETLEPVPLSPYGITKLTGEHLCRVYFREFAVPVVILRYFTVY
HHHHCCHHHCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GPRQRPDMSFHRFIRQLLAGQPLTVFGDGTQSRDFTYISDCIDGTAAALERDHVIGETIN
CCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEC
IGGKERASVNDVIHLLEVLTGRKATIHYTSAARGEPKQTWADLTKAEQLLAYEPTITLID
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
GLQKEIEYIRSLYKGEQP
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNILVTGAAGFIGSHLCEKLLENDQHQVIGVDGFIGPTPAPLKMKNIAHLQSHPRFTLVE
CCEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEE
TDLLTADLPALLKDVEAVYHLAGMPGVRTSWGTDFADYAVHNISVTQRLLEACKDLPLKR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
FIYASTSSVYGERSGPLSETLEPVPLSPYGITKLTGEHLCRVYFREFAVPVVILRYFTVY
HHHHCCHHHCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GPRQRPDMSFHRFIRQLLAGQPLTVFGDGTQSRDFTYISDCIDGTAAALERDHVIGETIN
CCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEC
IGGKERASVNDVIHLLEVLTGRKATIHYTSAARGEPKQTWADLTKAEQLLAYEPTITLID
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
GLQKEIEYIRSLYKGEQP
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]