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The map label for this gene is oppA [H]

Identifier: 138894363

GI number: 138894363

Start: 770595

End: 772109

Strand: Direct

Name: oppA [H]

Synonym: GTNG_0691

Alternate gene names: 138894363

Gene position: 770595-772109 (Clockwise)

Preceding gene: 138894360

Following gene: 138894364

Centisome position: 21.7

GC content: 46.6

Gene sequence:

>1515_bases
GTGCTGAACTTGCTTGATTCTTCAGAAATTCCGTCGCTTGACTCAGCGCTCGCTACCGACCAAGTATCGTTTATCGTGTT
GAACAACGTCATGGAAGGTCTGTACCGTTTAGGAAAAGACAACAAGCCGGTTCCTGGGGTGGCGGAAAGCCATGAGGTGA
GCGAAGACGGCAAAACGTATACGTTCAAGCTTCGCAAAGATGCTAAATGGTCGAACGGTGACCCGGTGACGGCGCATGAC
TTTGTGTTCGCTTGGAAACGGGTGCTTGATCCGAAAACGAAGTCTGAATATGCGTACATCATGTACGACATTAAAAATGC
GGAAGAAGTCAACACCGGCAAATTGCCAGTTGACCAGCTCGGTGTCAAAGCGCTTGACGACTACACGCTCCAAGTGGAGC
TGAAAAACCCGGTTCCGTATTTCGTCAGCTTGACGGTATTCGGTTCGTTCATGCCGCAAAACGAAAAATTCGTGACAGCT
CAAGGCGACAAGTACGGTTTGGAAGCGAACACGACGCTCTACAACGGTCCGTTCGTCTTAAGCGAATGGAAACACGAGCA
AGGCTGGACATATGAGAAAAACCCGAATTACTGGGATAAAGATACAGTTAAGCTCGAAAAAATCAATGTGAAAGTCGTAA
AAGACACCGCAACGGCTGTCAACTTGTATGAAACGAAACAAGTTGACCGTGTCGGTTTGTCGGCTGAATTTGTTGATAAG
TATAAAAACGACAAAAACTTCAAAACGGAATTGGAGCCATCTCTCTTCTGGCTGCGCATGAACACGAAAAATGAGCCGTT
AAACAATGTGAATGCGCGCAAAGCAATTGCGATGGCCATCGACCGCCAAGCAATGGTGGATACGCTGCTTAACAACGGTT
CGATTGTAGCGAACTTTACTGTCCCGAAAGATTTCGTTACAGGCCCGGATGGCAAAGACTTCCGCGATGCAAACGGTGAC
TTAGTGTCGTACAATGTGGACGAAGCGAAAAAACTGTGGGAGCAAGCGAAAAAAGAGCTTGGCAAAGACAATTTCACGAT
TGAGCTGTTAAACTATGACAGCGACAGCTCGAAGAAAATTGGCGAGTACATCAAAGAAGAATTGGAGAAAAACTTGGAGG
GCTTGACGGTCAACATTAAACAGCAACCGTTCCAACAAAAACTTGACCTCGAAAGCAAAATGCAATTTGACTTGTCATTC
TCTGGTTGGGGTCCGGACTATCAAGACCCGATGACGTTCCTTGATTTGTGGACGACAAATAACCCGCACAACCAAACGGG
TTGGTCGAATGCTGAATACGACAAGTTGATTAAAGATGCAAAAACAACGTTGCTTGGCGATTTGCAAGCTCGTTGGGATG
CGATGCTGAAAGCAGAAAAGATCGTCTTTGAAGAAATGCCGATTGCTCCGCTCTATCAGCGTGGCCTCGCTTACCTAGAA
CGCGAGTATGTAAAAGATATCATTACTCACCCGTTTGGCGGCGACTACAGCTACAAATGGGCCTATATCGAGTAA

Upstream 100 bases:

>100_bases
TCTCGCGCTGTCTACGTTTTTAGCAGCGTGTGGCGGGGGCGGGGACGACAAAGCCCAAGGCGGTGAAAGCGGCGAAAAGC
CGGCGGAGAAGAAGGAGCAA

Downstream 100 bases:

>100_bases
TTCGTTTTCTAAAGAATGCCAAGGAGAGTATATGCGAGGGCATATACTCTCTTTTTGGCTGTCAAGGCGAAAAATGTCGA
AAAAAGCAAAATGTTAAGAG

Product: oligopeptide-binding protein OppA

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MLNLLDSSEIPSLDSALATDQVSFIVLNNVMEGLYRLGKDNKPVPGVAESHEVSEDGKTYTFKLRKDAKWSNGDPVTAHD
FVFAWKRVLDPKTKSEYAYIMYDIKNAEEVNTGKLPVDQLGVKALDDYTLQVELKNPVPYFVSLTVFGSFMPQNEKFVTA
QGDKYGLEANTTLYNGPFVLSEWKHEQGWTYEKNPNYWDKDTVKLEKINVKVVKDTATAVNLYETKQVDRVGLSAEFVDK
YKNDKNFKTELEPSLFWLRMNTKNEPLNNVNARKAIAMAIDRQAMVDTLLNNGSIVANFTVPKDFVTGPDGKDFRDANGD
LVSYNVDEAKKLWEQAKKELGKDNFTIELLNYDSDSSKKIGEYIKEELEKNLEGLTVNIKQQPFQQKLDLESKMQFDLSF
SGWGPDYQDPMTFLDLWTTNNPHNQTGWSNAEYDKLIKDAKTTLLGDLQARWDAMLKAEKIVFEEMPIAPLYQRGLAYLE
REYVKDIITHPFGGDYSYKWAYIE

Sequences:

>Translated_504_residues
MLNLLDSSEIPSLDSALATDQVSFIVLNNVMEGLYRLGKDNKPVPGVAESHEVSEDGKTYTFKLRKDAKWSNGDPVTAHD
FVFAWKRVLDPKTKSEYAYIMYDIKNAEEVNTGKLPVDQLGVKALDDYTLQVELKNPVPYFVSLTVFGSFMPQNEKFVTA
QGDKYGLEANTTLYNGPFVLSEWKHEQGWTYEKNPNYWDKDTVKLEKINVKVVKDTATAVNLYETKQVDRVGLSAEFVDK
YKNDKNFKTELEPSLFWLRMNTKNEPLNNVNARKAIAMAIDRQAMVDTLLNNGSIVANFTVPKDFVTGPDGKDFRDANGD
LVSYNVDEAKKLWEQAKKELGKDNFTIELLNYDSDSSKKIGEYIKEELEKNLEGLTVNIKQQPFQQKLDLESKMQFDLSF
SGWGPDYQDPMTFLDLWTTNNPHNQTGWSNAEYDKLIKDAKTTLLGDLQARWDAMLKAEKIVFEEMPIAPLYQRGLAYLE
REYVKDIITHPFGGDYSYKWAYIE
>Mature_504_residues
MLNLLDSSEIPSLDSALATDQVSFIVLNNVMEGLYRLGKDNKPVPGVAESHEVSEDGKTYTFKLRKDAKWSNGDPVTAHD
FVFAWKRVLDPKTKSEYAYIMYDIKNAEEVNTGKLPVDQLGVKALDDYTLQVELKNPVPYFVSLTVFGSFMPQNEKFVTA
QGDKYGLEANTTLYNGPFVLSEWKHEQGWTYEKNPNYWDKDTVKLEKINVKVVKDTATAVNLYETKQVDRVGLSAEFVDK
YKNDKNFKTELEPSLFWLRMNTKNEPLNNVNARKAIAMAIDRQAMVDTLLNNGSIVANFTVPKDFVTGPDGKDFRDANGD
LVSYNVDEAKKLWEQAKKELGKDNFTIELLNYDSDSSKKIGEYIKEELEKNLEGLTVNIKQQPFQQKLDLESKMQFDLSF
SGWGPDYQDPMTFLDLWTTNNPHNQTGWSNAEYDKLIKDAKTTLLGDLQARWDAMLKAEKIVFEEMPIAPLYQRGLAYLE
REYVKDIITHPFGGDYSYKWAYIE

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=503, Percent_Identity=31.4115308151093, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789397, Length=493, Percent_Identity=29.2089249492901, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI87081878, Length=484, Percent_Identity=31.198347107438, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1787762, Length=459, Percent_Identity=24.8366013071895, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1787551, Length=487, Percent_Identity=23.8193018480493, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789966, Length=504, Percent_Identity=23.6111111111111, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789887, Length=408, Percent_Identity=25.9803921568627, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787052, Length=484, Percent_Identity=24.1735537190083, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57728; Mature: 57728

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLLDSSEIPSLDSALATDQVSFIVLNNVMEGLYRLGKDNKPVPGVAESHEVSEDGKTY
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
TFKLRKDAKWSNGDPVTAHDFVFAWKRVLDPKTKSEYAYIMYDIKNAEEVNTGKLPVDQL
EEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHH
GVKALDDYTLQVELKNPVPYFVSLTVFGSFMPQNEKFVTAQGDKYGLEANTTLYNGPFVL
CCEEECCEEEEEEECCCCCEEEEEEEHHCCCCCCCEEEEECCCCCCCCCCCEEECCCEEE
SEWKHEQGWTYEKNPNYWDKDTVKLEKINVKVVKDTATAVNLYETKQVDRVGLSAEFVDK
ECCCCCCCCEECCCCCCCCCCCEEEEEEEEEEEECCCCEEEEHHHHHHHHCCCCHHHHHH
YKNDKNFKTELEPSLFWLRMNTKNEPLNNVNARKAIAMAIDRQAMVDTLLNNGSIVANFT
HCCCCCCCEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
VPKDFVTGPDGKDFRDANGDLVSYNVDEAKKLWEQAKKELGKDNFTIELLNYDSDSSKKI
CCHHHCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHH
GEYIKEELEKNLEGLTVNIKQQPFQQKLDLESKMQFDLSFSGWGPDYQDPMTFLDLWTTN
HHHHHHHHHCCCCCEEEEECCCCHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCC
NPHNQTGWSNAEYDKLIKDAKTTLLGDLQARWDAMLKAEKIVFEEMPIAPLYQRGLAYLE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
REYVKDIITHPFGGDYSYKWAYIE
HHHHHHHHCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MLNLLDSSEIPSLDSALATDQVSFIVLNNVMEGLYRLGKDNKPVPGVAESHEVSEDGKTY
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
TFKLRKDAKWSNGDPVTAHDFVFAWKRVLDPKTKSEYAYIMYDIKNAEEVNTGKLPVDQL
EEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHH
GVKALDDYTLQVELKNPVPYFVSLTVFGSFMPQNEKFVTAQGDKYGLEANTTLYNGPFVL
CCEEECCEEEEEEECCCCCEEEEEEEHHCCCCCCCEEEEECCCCCCCCCCCEEECCCEEE
SEWKHEQGWTYEKNPNYWDKDTVKLEKINVKVVKDTATAVNLYETKQVDRVGLSAEFVDK
ECCCCCCCCEECCCCCCCCCCCEEEEEEEEEEEECCCCEEEEHHHHHHHHCCCCHHHHHH
YKNDKNFKTELEPSLFWLRMNTKNEPLNNVNARKAIAMAIDRQAMVDTLLNNGSIVANFT
HCCCCCCCEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
VPKDFVTGPDGKDFRDANGDLVSYNVDEAKKLWEQAKKELGKDNFTIELLNYDSDSSKKI
CCHHHCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHH
GEYIKEELEKNLEGLTVNIKQQPFQQKLDLESKMQFDLSFSGWGPDYQDPMTFLDLWTTN
HHHHHHHHHCCCCCEEEEECCCCHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCC
NPHNQTGWSNAEYDKLIKDAKTTLLGDLQARWDAMLKAEKIVFEEMPIAPLYQRGLAYLE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
REYVKDIITHPFGGDYSYKWAYIE
HHHHHHHHCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]