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The map label for this gene is narH [H]

Identifier: 138894326

GI number: 138894326

Start: 733509

End: 735041

Strand: Direct

Name: narH [H]

Synonym: GTNG_0654

Alternate gene names: 138894326

Gene position: 733509-735041 (Clockwise)

Preceding gene: 138894325

Following gene: 138894327

Centisome position: 20.66

GC content: 49.97

Gene sequence:

>1533_bases
TTGAGAGTTAGAGCGCAGTTTGGGATGGTCATGAATTTGGACAAGTGCATCGGTTGCCACACGTGTAGCATTACATGCAA
CAATACATGGACGAATCGTCCCGGGGCGGAATATATGTGGTGGAACAACGTGGAAACGAAACCGGGCATCGGCTATCCGA
AAGAATGGGAAAACCAAGATGCGCATAAAGGAGGCTGGGTGCTCAAAGACGGGAAGCTCGAGCTACGCGCTGGCGGCCGG
GTGAATAAACTGTTAAACATTTTTTACAACCCCGATATGACGGTGATCGACGATTACTATGAGCCTTGGACATACGATTA
TGAACAGCTGATCAACCGCCCGGAAGGGGAGCACCAGCCTGTCGCCCGGCCTAAGTCGCAGCTGACTGGCGAATACATGG
ACATCACGTGGGGGCCGAACTGGGAAGACGATTTGGCCGGGGTATATGAAACAGGAAAACGCGACCCGAACGTAAGAGGC
ATTGAAGAGAAAGTGAAATTTGAGTTCGAACAAACATTTATGATGTATTTGCCGCGCATTTGCGAACATTGTTTAAACCC
GCCTTGCGTCTCTTCTTGTCCATCAGGGGCGATTTATAAGCGAGATGAGGACGGCATTGTGCTCGTTGATCATGATGCGT
GCCGGAGTTGGCGGTTTTGTGTGACCGGCTGTCCATATAAAAAAGTGTACTTTAATTGGAAAACGCATAAGGCAGAGAAA
TGTACATTTTGTTTTCCGCGTATTGAAGCAGGATTGCCGACGGTTTGTTCAGAAACGTGCGTCGGCCGTCTCCGTTACAT
CGGCATTGTGTTTTACGATTTGGATCAAGTTGAGGCGGCCGCTTCGGTCCAAGATGAAAAGGAGCTGTACCACGCCCATT
TGCGCATCTTTTTAAATCCGTATGATCCGGACGTGATTGAAGCGGCACGTGAAGCGGGGTATACGGACGATTGGATTGAA
GCAGCGCAACGCTCACCGGTTTATAAGCTTGCCGTCGAGCAAAAGATCGCGCTCCCGCTTCATCCGGAATACCGGACGTT
GCCGATGGTTTGGTATATTCCACCGCTCAGCCCGATCATGGGGGCGTTTGACGGTGGACTGGACGACATTCATCCGCATG
TGATTTTCCCGGCGATTGACCAAATGCGCATCCCGATTGAGTATTTGGCTAATTTGTTGGCTGCCGGCGACACTGAGGTC
ATCCGCACGGTGTTGAAAAAAATGGTGGCCATGCGCAGCTATATGCGGGCGGTGAACTTAGGAAAAGAACCGGACCGGAG
TATTCTCGAGAAAGTCGGAATGGATGAACATACGGTCAAGGAAATGTATAAGCTTTCGGCTGTCGCGAAATATTCCGACC
GCTATGTCATTCCATCTTCCCATCGGGAGAAAGCCGGCAATGCGTTTTACAGTCAAGGGACAGCGGGCTATGCGTTTATG
GAGTCTTGTTCCGGCTGCTCTGGATATGATGCGGAAAGTTTTTACCAATTTTATTGGGGGGATGAGGATGGAAGAGCATC
AGAAACTATTTAA

Upstream 100 bases:

>100_bases
GCGCAATTAAGCTACGGCTTCAATTATTATGGGCCGACAGGAAACCAGCGGGACGAGCAAGTGATGATCCGCAAACTTGA
AAAAAGGGAAGTGGATTGGC

Downstream 100 bases:

>100_bases
GCTTGCTTCCATTTTGTTGCAATATCCGGAACGAGAATGGCTCGAAGACGACCGGCTGTTTTATGAAATTTCTCAGCTCG
CCAACCATACCGCTCGCAAA

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MRVRAQFGMVMNLDKCIGCHTCSITCNNTWTNRPGAEYMWWNNVETKPGIGYPKEWENQDAHKGGWVLKDGKLELRAGGR
VNKLLNIFYNPDMTVIDDYYEPWTYDYEQLINRPEGEHQPVARPKSQLTGEYMDITWGPNWEDDLAGVYETGKRDPNVRG
IEEKVKFEFEQTFMMYLPRICEHCLNPPCVSSCPSGAIYKRDEDGIVLVDHDACRSWRFCVTGCPYKKVYFNWKTHKAEK
CTFCFPRIEAGLPTVCSETCVGRLRYIGIVFYDLDQVEAAASVQDEKELYHAHLRIFLNPYDPDVIEAAREAGYTDDWIE
AAQRSPVYKLAVEQKIALPLHPEYRTLPMVWYIPPLSPIMGAFDGGLDDIHPHVIFPAIDQMRIPIEYLANLLAAGDTEV
IRTVLKKMVAMRSYMRAVNLGKEPDRSILEKVGMDEHTVKEMYKLSAVAKYSDRYVIPSSHREKAGNAFYSQGTAGYAFM
ESCSGCSGYDAESFYQFYWGDEDGRASETI

Sequences:

>Translated_510_residues
MRVRAQFGMVMNLDKCIGCHTCSITCNNTWTNRPGAEYMWWNNVETKPGIGYPKEWENQDAHKGGWVLKDGKLELRAGGR
VNKLLNIFYNPDMTVIDDYYEPWTYDYEQLINRPEGEHQPVARPKSQLTGEYMDITWGPNWEDDLAGVYETGKRDPNVRG
IEEKVKFEFEQTFMMYLPRICEHCLNPPCVSSCPSGAIYKRDEDGIVLVDHDACRSWRFCVTGCPYKKVYFNWKTHKAEK
CTFCFPRIEAGLPTVCSETCVGRLRYIGIVFYDLDQVEAAASVQDEKELYHAHLRIFLNPYDPDVIEAAREAGYTDDWIE
AAQRSPVYKLAVEQKIALPLHPEYRTLPMVWYIPPLSPIMGAFDGGLDDIHPHVIFPAIDQMRIPIEYLANLLAAGDTEV
IRTVLKKMVAMRSYMRAVNLGKEPDRSILEKVGMDEHTVKEMYKLSAVAKYSDRYVIPSSHREKAGNAFYSQGTAGYAFM
ESCSGCSGYDAESFYQFYWGDEDGRASETI
>Mature_510_residues
MRVRAQFGMVMNLDKCIGCHTCSITCNNTWTNRPGAEYMWWNNVETKPGIGYPKEWENQDAHKGGWVLKDGKLELRAGGR
VNKLLNIFYNPDMTVIDDYYEPWTYDYEQLINRPEGEHQPVARPKSQLTGEYMDITWGPNWEDDLAGVYETGKRDPNVRG
IEEKVKFEFEQTFMMYLPRICEHCLNPPCVSSCPSGAIYKRDEDGIVLVDHDACRSWRFCVTGCPYKKVYFNWKTHKAEK
CTFCFPRIEAGLPTVCSETCVGRLRYIGIVFYDLDQVEAAASVQDEKELYHAHLRIFLNPYDPDVIEAAREAGYTDDWIE
AAQRSPVYKLAVEQKIALPLHPEYRTLPMVWYIPPLSPIMGAFDGGLDDIHPHVIFPAIDQMRIPIEYLANLLAAGDTEV
IRTVLKKMVAMRSYMRAVNLGKEPDRSILEKVGMDEHTVKEMYKLSAVAKYSDRYVIPSSHREKAGNAFYSQGTAGYAFM
ESCSGCSGYDAESFYQFYWGDEDGRASETI

Specific function: The Nitrate Reductase Enzyme Complex Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In The Formation Of Iron-Sulfur Centers. Electrons A

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=488, Percent_Identity=58.4016393442623, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1787740, Length=488, Percent_Identity=56.9672131147541, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI1787872, Length=101, Percent_Identity=38.6138613861386, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=38.6138613861386, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI226510944, Length=118, Percent_Identity=34.7457627118644, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI2367345, Length=142, Percent_Identity=29.5774647887324, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789370, Length=102, Percent_Identity=34.3137254901961, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58450; Mature: 58450

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRAQFGMVMNLDKCIGCHTCSITCNNTWTNRPGAEYMWWNNVETKPGIGYPKEWENQD
CCCCCHHCCCEEHHHHCCEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
AHKGGWVLKDGKLELRAGGRVNKLLNIFYNPDMTVIDDYYEPWTYDYEQLINRPEGEHQP
CCCCCEEEECCCEEEECCCCHHHHHHEEECCCCEEECCCCCCCCCCHHHHCCCCCCCCCC
VARPKSQLTGEYMDITWGPNWEDDLAGVYETGKRDPNVRGIEEKVKFEFEQTFMMYLPRI
CCCCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
CEHCLNPPCVSSCPSGAIYKRDEDGIVLVDHDACRSWRFCVTGCPYKKVYFNWKTHKAEK
HHHHCCCCCHHCCCCCCCEEECCCCEEEEECHHCCCCCEEECCCCCEEEEEEEECCCCCC
CTFCFPRIEAGLPTVCSETCVGRLRYIGIVFYDLDQVEAAASVQDEKELYHAHLRIFLNP
CEEECHHHHCCCCHHHHHHHHHHHHEEEEEEEEHHHHHHHHHHHHHHHHHEEEEEEEECC
YDPDVIEAAREAGYTDDWIEAAQRSPVYKLAVEQKIALPLHPEYRTLPMVWYIPPLSPIM
CCCHHHHHHHHCCCCHHHHHHHHCCCEEEEEHHCEEECCCCCCCCCCCEEEECCCHHHHH
GAFDGGLDDIHPHVIFPAIDQMRIPIEYLANLLAAGDTEVIRTVLKKMVAMRSYMRAVNL
HHHCCCCCCCCCEEEECCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
GKEPDRSILEKVGMDEHTVKEMYKLSAVAKYSDRYVIPSSHREKAGNAFYSQGTAGYAFM
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHHHCCCHHHCCCCCCHHHH
ESCSGCSGYDAESFYQFYWGDEDGRASETI
HHCCCCCCCCHHHHEEEEECCCCCCCCCCC
>Mature Secondary Structure
MRVRAQFGMVMNLDKCIGCHTCSITCNNTWTNRPGAEYMWWNNVETKPGIGYPKEWENQD
CCCCCHHCCCEEHHHHCCEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
AHKGGWVLKDGKLELRAGGRVNKLLNIFYNPDMTVIDDYYEPWTYDYEQLINRPEGEHQP
CCCCCEEEECCCEEEECCCCHHHHHHEEECCCCEEECCCCCCCCCCHHHHCCCCCCCCCC
VARPKSQLTGEYMDITWGPNWEDDLAGVYETGKRDPNVRGIEEKVKFEFEQTFMMYLPRI
CCCCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
CEHCLNPPCVSSCPSGAIYKRDEDGIVLVDHDACRSWRFCVTGCPYKKVYFNWKTHKAEK
HHHHCCCCCHHCCCCCCCEEECCCCEEEEECHHCCCCCEEECCCCCEEEEEEEECCCCCC
CTFCFPRIEAGLPTVCSETCVGRLRYIGIVFYDLDQVEAAASVQDEKELYHAHLRIFLNP
CEEECHHHHCCCCHHHHHHHHHHHHEEEEEEEEHHHHHHHHHHHHHHHHHEEEEEEEECC
YDPDVIEAAREAGYTDDWIEAAQRSPVYKLAVEQKIALPLHPEYRTLPMVWYIPPLSPIM
CCCHHHHHHHHCCCCHHHHHHHHCCCEEEEEHHCEEECCCCCCCCCCCEEEECCCHHHHH
GAFDGGLDDIHPHVIFPAIDQMRIPIEYLANLLAAGDTEVIRTVLKKMVAMRSYMRAVNL
HHHCCCCCCCCCEEEECCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
GKEPDRSILEKVGMDEHTVKEMYKLSAVAKYSDRYVIPSSHREKAGNAFYSQGTAGYAFM
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHHHCCCHHHCCCCCCHHHH
ESCSGCSGYDAESFYQFYWGDEDGRASETI
HHCCCCCCCCHHHHEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]