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The map label for this gene is asnO [H]

Identifier: 138894281

GI number: 138894281

Start: 671821

End: 673668

Strand: Direct

Name: asnO [H]

Synonym: GTNG_0607

Alternate gene names: 138894281

Gene position: 671821-673668 (Clockwise)

Preceding gene: 138894279

Following gene: 138894283

Centisome position: 18.92

GC content: 49.78

Gene sequence:

>1848_bases
ATGTGTGGCATCACTGGCTGGGTGCATTTCGGGCGTGATTTGCGCCGTAAGCAAGAGGTGATTACCAGGATGACGGAGAC
GCTCGCTAAACGTGGACCGGACGATACGAATACATGGCTCGACACTCATGCTGCGTTCGGTCATAAGCGGCTCGTCGTTA
TTGACCCGGCCGGCGGCAAACAGCCGATGATCCGGAACAAAAACGGACACCGCTACACGATCGTTTACAACGGTGAGCTA
TACAATACGGAAGACATCCGTAAAGAACTGTTGCACAAAGGCTACCGGTTTGACGGCCATTCTGATACAGAAGTGCTGCT
CGCCGCTTATATAGAGTGGAAAGAACAATGTGTCGATCGGTTAAACGGTATTTTTGCCTTTGCTGTTTGGGATGAGGAGC
GGGAACTGTTATTTATGGCGCGTGACCGTCTTGGAGTCAAACCATTGTTTTACCGTCAACAGGCTGAAGAGTTGCTGTTC
GGCTCCGAAGTGAAGGCAATTTTAGCTCACCCTGATGTAAAAGCAGAAGTCAGTTATGAAGGGTTAGCGGAGGTATTTGG
GCTCGGTCCGTCGCGCACACCGGGACATGGAGTATTTTATGGTGTCCAGGAACTGCGCCCGGCGCACGCGTTAACCTTCT
CCCGTACCGGGCTTCGCGTCTGGCGGTACTGGAACGTCGAAAGCGATGTCCATCGTGATTCGCTCGGGGAAACGATCGAG
AAGCTGCGTTTTTTATTGACCGATGCTGTCACACGCCAGCTCGTTTCCGATGTGCCGGTATGCACCTTTTTGTCGGGCGG
CGTTGATTCAAGCGCCATTACAGCGATTGCCGCCAACGCTTTCGCCGCTAACGGGAAAGGACCGCTCCATACGTACTCGA
TCGATTATGAAGGAAACGATCAATATTTTCAGGCGAACGATTTTCAGCCAAATACAGATGCGCCGTTTATTGAACAAGTG
TCAAACAAGTTTCAAACGATCCACCATCGCTGCGTGATCACTCAAGAAGAGCTGTTCCAACATTTGCGTGATGCAGTGAT
CGTCCGTGACGTTCCAGGCATGGCTGATGTCGATTCTTCGCTCCTTTGGTTTTGTAAACAAATTCGTGAGCAGTTTGTCG
TGAGTTTATCTGGAGAATGTGCGGATGAAATTTTCGGCGGCTATCCGTGGTTTCATCGTCCAGATGACTTAGCAAGAAAA
GGGTTTCCGTGGATGCGCTCGATTGAAGCTCGCATTGGCTTGTTAAAAGAAGAATGGCGGAAAAAACTTCGCCTTGACGA
CTACGTGCAAATGCGTTATGAAGAAACGCTTGTTGAAGTGCCGCGTCTTGAAGGGGAAAGTGCCGAGGCGGCGAAGCGGC
GTGAACTGTTTTACTTAAATATGATTTGGTTTATGACGACACTGCTTGACCGGAAAGACCGAATGAGCATGGGGGCGAGC
CTTGAGGTACGTGTGCCGTTTGCTGATCACCGGCTCGTTGAATATGTTTGGAATATCCCATGGGAAATGAAAATGTACGG
AGGACGAGAAAAAGGTATTTTGCGCAAAGCACTTGAAGGGCTGCTTCCGCAAGACGTGCTCTATCGAAAGAAAAGTCCGT
ACCCGAAAACACACCATCCGCTGTATACGAAGTTGGTGAAGCAATGGCTCAAGCAACTGCTTCGTGATCGTTCTTCTATC
TTGCACGAGTTTTTCGATCAAGAAAAACTGACGGCGCTTGTCGAATCGGGAGGGAAGTCGTTCCAAGTGCCATGGTTTGG
GCAACTCATGACCGGACCACAGCTGCTTGCTTATCTTGGCCAGGTGCATGTTTGGTTTGACCATTACGGCATTCATATTA
AAGAATAA

Upstream 100 bases:

>100_bases
CGCGAGGAAAAAGGGGAACGGGCGATCGAAAGAACAATGTGAACGGTTCGGGCGAACAAATATTTATTTGCCATTTTTGA
TAAAGAAAGGATTGGTTCGT

Downstream 100 bases:

>100_bases
GAGCTGCGTGAGGACGCAGCTCTTATTCTATTGAATAGCAAAGAGAGCAGTACTATGCCATTTTCGCTTGCGGCGCCACC
GCTTTTTTCATCCAGATGCG

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MCGITGWVHFGRDLRRKQEVITRMTETLAKRGPDDTNTWLDTHAAFGHKRLVVIDPAGGKQPMIRNKNGHRYTIVYNGEL
YNTEDIRKELLHKGYRFDGHSDTEVLLAAYIEWKEQCVDRLNGIFAFAVWDEERELLFMARDRLGVKPLFYRQQAEELLF
GSEVKAILAHPDVKAEVSYEGLAEVFGLGPSRTPGHGVFYGVQELRPAHALTFSRTGLRVWRYWNVESDVHRDSLGETIE
KLRFLLTDAVTRQLVSDVPVCTFLSGGVDSSAITAIAANAFAANGKGPLHTYSIDYEGNDQYFQANDFQPNTDAPFIEQV
SNKFQTIHHRCVITQEELFQHLRDAVIVRDVPGMADVDSSLLWFCKQIREQFVVSLSGECADEIFGGYPWFHRPDDLARK
GFPWMRSIEARIGLLKEEWRKKLRLDDYVQMRYEETLVEVPRLEGESAEAAKRRELFYLNMIWFMTTLLDRKDRMSMGAS
LEVRVPFADHRLVEYVWNIPWEMKMYGGREKGILRKALEGLLPQDVLYRKKSPYPKTHHPLYTKLVKQWLKQLLRDRSSI
LHEFFDQEKLTALVESGGKSFQVPWFGQLMTGPQLLAYLGQVHVWFDHYGIHIKE

Sequences:

>Translated_615_residues
MCGITGWVHFGRDLRRKQEVITRMTETLAKRGPDDTNTWLDTHAAFGHKRLVVIDPAGGKQPMIRNKNGHRYTIVYNGEL
YNTEDIRKELLHKGYRFDGHSDTEVLLAAYIEWKEQCVDRLNGIFAFAVWDEERELLFMARDRLGVKPLFYRQQAEELLF
GSEVKAILAHPDVKAEVSYEGLAEVFGLGPSRTPGHGVFYGVQELRPAHALTFSRTGLRVWRYWNVESDVHRDSLGETIE
KLRFLLTDAVTRQLVSDVPVCTFLSGGVDSSAITAIAANAFAANGKGPLHTYSIDYEGNDQYFQANDFQPNTDAPFIEQV
SNKFQTIHHRCVITQEELFQHLRDAVIVRDVPGMADVDSSLLWFCKQIREQFVVSLSGECADEIFGGYPWFHRPDDLARK
GFPWMRSIEARIGLLKEEWRKKLRLDDYVQMRYEETLVEVPRLEGESAEAAKRRELFYLNMIWFMTTLLDRKDRMSMGAS
LEVRVPFADHRLVEYVWNIPWEMKMYGGREKGILRKALEGLLPQDVLYRKKSPYPKTHHPLYTKLVKQWLKQLLRDRSSI
LHEFFDQEKLTALVESGGKSFQVPWFGQLMTGPQLLAYLGQVHVWFDHYGIHIKE
>Mature_615_residues
MCGITGWVHFGRDLRRKQEVITRMTETLAKRGPDDTNTWLDTHAAFGHKRLVVIDPAGGKQPMIRNKNGHRYTIVYNGEL
YNTEDIRKELLHKGYRFDGHSDTEVLLAAYIEWKEQCVDRLNGIFAFAVWDEERELLFMARDRLGVKPLFYRQQAEELLF
GSEVKAILAHPDVKAEVSYEGLAEVFGLGPSRTPGHGVFYGVQELRPAHALTFSRTGLRVWRYWNVESDVHRDSLGETIE
KLRFLLTDAVTRQLVSDVPVCTFLSGGVDSSAITAIAANAFAANGKGPLHTYSIDYEGNDQYFQANDFQPNTDAPFIEQV
SNKFQTIHHRCVITQEELFQHLRDAVIVRDVPGMADVDSSLLWFCKQIREQFVVSLSGECADEIFGGYPWFHRPDDLARK
GFPWMRSIEARIGLLKEEWRKKLRLDDYVQMRYEETLVEVPRLEGESAEAAKRRELFYLNMIWFMTTLLDRKDRMSMGAS
LEVRVPFADHRLVEYVWNIPWEMKMYGGREKGILRKALEGLLPQDVLYRKKSPYPKTHHPLYTKLVKQWLKQLLRDRSSI
LHEFFDQEKLTALVESGGKSFQVPWFGQLMTGPQLLAYLGQVHVWFDHYGIHIKE

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=603, Percent_Identity=26.2023217247098, Blast_Score=149, Evalue=1e-35,
Organism=Homo sapiens, GI168229252, Length=603, Percent_Identity=26.2023217247098, Blast_Score=149, Evalue=1e-35,
Organism=Homo sapiens, GI168229250, Length=603, Percent_Identity=26.2023217247098, Blast_Score=149, Evalue=1e-35,
Organism=Homo sapiens, GI296010848, Length=576, Percent_Identity=25.8680555555556, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI296010852, Length=507, Percent_Identity=24.2603550295858, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI296010850, Length=507, Percent_Identity=24.2603550295858, Blast_Score=106, Evalue=6e-23,
Organism=Escherichia coli, GI1786889, Length=556, Percent_Identity=27.3381294964029, Blast_Score=159, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI71993933, Length=555, Percent_Identity=25.045045045045, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=555, Percent_Identity=25.045045045045, Blast_Score=121, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6321563, Length=570, Percent_Identity=25.2631578947368, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325403, Length=571, Percent_Identity=24.6935201401051, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45553209, Length=567, Percent_Identity=25.5731922398589, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 71212; Mature: 71212

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGWVHFGRDLRRKQEVITRMTETLAKRGPDDTNTWLDTHAAFGHKRLVVIDPAGGK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCC
QPMIRNKNGHRYTIVYNGELYNTEDIRKELLHKGYRFDGHSDTEVLLAAYIEWKEQCVDR
CCCEECCCCCEEEEEECCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFAVWDEERELLFMARDRLGVKPLFYRQQAEELLFGSEVKAILAHPDVKAEVSYE
HCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCEECCHH
GLAEVFGLGPSRTPGHGVFYGVQELRPAHALTFSRTGLRVWRYWNVESDVHRDSLGETIE
HHHHHHCCCCCCCCCCCEEEEHHHCCCHHHEEEECCCEEEEEEECCCCHHHHHHHHHHHH
KLRFLLTDAVTRQLVSDVPVCTFLSGGVDSSAITAIAANAFAANGKGPLHTYSIDYEGND
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
QYFQANDFQPNTDAPFIEQVSNKFQTIHHRCVITQEELFQHLRDAVIVRDVPGMADVDSS
CEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHEEEECCCCCCCCHHH
LLWFCKQIREQFVVSLSGECADEIFGGYPWFHRPDDLARKGFPWMRSIEARIGLLKEEWR
HHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHH
KKLRLDDYVQMRYEETLVEVPRLEGESAEAAKRRELFYLNMIWFMTTLLDRKDRMSMGAS
HHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
LEVRVPFADHRLVEYVWNIPWEMKMYGGREKGILRKALEGLLPQDVLYRKKSPYPKTHHP
EEEECCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCH
LYTKLVKQWLKQLLRDRSSILHEFFDQEKLTALVESGGKSFQVPWFGQLMTGPQLLAYLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCHHHHHHHH
QVHVWFDHYGIHIKE
HHEEEEEECCEEECC
>Mature Secondary Structure
MCGITGWVHFGRDLRRKQEVITRMTETLAKRGPDDTNTWLDTHAAFGHKRLVVIDPAGGK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCC
QPMIRNKNGHRYTIVYNGELYNTEDIRKELLHKGYRFDGHSDTEVLLAAYIEWKEQCVDR
CCCEECCCCCEEEEEECCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFAVWDEERELLFMARDRLGVKPLFYRQQAEELLFGSEVKAILAHPDVKAEVSYE
HCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCEECCHH
GLAEVFGLGPSRTPGHGVFYGVQELRPAHALTFSRTGLRVWRYWNVESDVHRDSLGETIE
HHHHHHCCCCCCCCCCCEEEEHHHCCCHHHEEEECCCEEEEEEECCCCHHHHHHHHHHHH
KLRFLLTDAVTRQLVSDVPVCTFLSGGVDSSAITAIAANAFAANGKGPLHTYSIDYEGND
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
QYFQANDFQPNTDAPFIEQVSNKFQTIHHRCVITQEELFQHLRDAVIVRDVPGMADVDSS
CEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHEEEECCCCCCCCHHH
LLWFCKQIREQFVVSLSGECADEIFGGYPWFHRPDDLARKGFPWMRSIEARIGLLKEEWR
HHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHH
KKLRLDDYVQMRYEETLVEVPRLEGESAEAAKRRELFYLNMIWFMTTLLDRKDRMSMGAS
HHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
LEVRVPFADHRLVEYVWNIPWEMKMYGGREKGILRKALEGLLPQDVLYRKKSPYPKTHHP
EEEECCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCH
LYTKLVKQWLKQLLRDRSSILHEFFDQEKLTALVESGGKSFQVPWFGQLMTGPQLLAYLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCHHHHHHHH
QVHVWFDHYGIHIKE
HHEEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]