Click here to switch to the map view.

The map label for this gene is dctA [H]

Identifier: 138894178

GI number: 138894178

Start: 567719

End: 568984

Strand: Direct

Name: dctA [H]

Synonym: GTNG_0504

Alternate gene names: 138894178

Gene position: 567719-568984 (Clockwise)

Preceding gene: 138894177

Following gene: 138894179

Centisome position: 15.99

GC content: 47.71

Gene sequence:

>1266_bases
ATGAGGGGGAAATTCAAAAATTTAACCGTACAAGTCATTATCGGTATTGTTTTAGGGATTATCGTCGGATTTTTATTTCC
GGAATTTGGTTCTAAATTAAAAGTGTTAGCTGATGGATTTATTAAGTTGATTAAAATGGTGATTGCCCCGATCATTTTCT
TTACCGTTGTGATCGGGATCGGCAATATGGGAGACTTGAAAAAAATTGGCCGCATCGGCGGAAAGGCCCTCCTTTACTTT
GAGATTGTGACGACGTTTGCGTTGGCGATTGGAATTGTTGTTGTTAACCTCATCAAACCAGGGGTTGGCTTCAACACTGA
TGCAGCCAAAGGTGGCGACATTTCACAATATACGAAGCAAGCGGAAGAAACAAGCCATGGGTTTGTTGATTTTGTGCTAA
GCATTATCCCGGATAACGTTGTCGCGGCGATGGCCAGCGGGGAATTGCTTCCGGTGCTCTTCTTCGCCGTTCTGTTTGGA
TTGGCGGCGGCTGGTTTAGGGGAAAAAGCCAAACCAGTGCTTTCCTTGTTTGAGCGGATCGCAGAAATTTTCTTTGGCGT
CGTCAACATGGTCATGAAGGTATCACCGATCGCCGCATTTGGTGCGATGGCGTATACGATCGGCACGTTCGGTCTCGGCT
CGCTCGTGTCGCTCGGCAAGCTGATGGGATCGGTATATATTACGATGGCGCTCTTTATTTTCATCGTGCTCGGCGGGATT
GCCAAGTTTTACGGATTTAACATCTTTAAGTTTCTCGCCTATATTAAAGAAGAATTGTTGCTCGTTCTTGGCACGTCTTC
ATCTGAATCGGCTTTGCCGCGGTTGATGGAGCGGCTTGAAAAATACGGTTGCTCGAAGTCGGTCGTTGGCCTTGTCGTTC
CGACTGGATACTCGTTTAACTTGGACGGCACATCGATTTATTTGTCGATGGCGGCCATTTTCATCGCCCAAGCATACGGG
ATTGATTTAAGCATTTGGCAAGAGTTGACGTTGCTTGGAGTGTTAATGCTCACCTCGAAGGGGGCAGCAGGGGTGACCGG
TTCGGGCTTTATCACCCTAGCCGCCACGTTAGCGGCGTTCCCAATGATTCCGGTCGAGGGTATCGCCTTGCTGCTTGGGG
TCGACCGTTTTATGTCCGAGGCGCGGGCGATTACAAACATTATTGGCAACGCAGTAGCCACGGTTGTCGTTTCGAAAATG
GAAAATGAGTTTCATCCTTCTGCTGAACAAAAACTAGCGGAAGGGAATATGACCATTGCGAAGTAA

Upstream 100 bases:

>100_bases
AGACCAAAATGACCATTATCACCATAATATTTTTATTTGGCTGACTTTTCTTTATGATGTTGTTGAAAGCGTTGTCAAGA
CGGTGAAAGGAGTGGGAGAA

Downstream 100 bases:

>100_bases
GAAACATGGGGCTGCTCCAAAAGGCCGCACAGTTGGCCTTTAGAGCAGCTTTTTTCTAGTACATTTAAGAAACGGAGTGG
ACTTCACTTTATGTTTCCGG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MRGKFKNLTVQVIIGIVLGIIVGFLFPEFGSKLKVLADGFIKLIKMVIAPIIFFTVVIGIGNMGDLKKIGRIGGKALLYF
EIVTTFALAIGIVVVNLIKPGVGFNTDAAKGGDISQYTKQAEETSHGFVDFVLSIIPDNVVAAMASGELLPVLFFAVLFG
LAAAGLGEKAKPVLSLFERIAEIFFGVVNMVMKVSPIAAFGAMAYTIGTFGLGSLVSLGKLMGSVYITMALFIFIVLGGI
AKFYGFNIFKFLAYIKEELLLVLGTSSSESALPRLMERLEKYGCSKSVVGLVVPTGYSFNLDGTSIYLSMAAIFIAQAYG
IDLSIWQELTLLGVLMLTSKGAAGVTGSGFITLAATLAAFPMIPVEGIALLLGVDRFMSEARAITNIIGNAVATVVVSKM
ENEFHPSAEQKLAEGNMTIAK

Sequences:

>Translated_421_residues
MRGKFKNLTVQVIIGIVLGIIVGFLFPEFGSKLKVLADGFIKLIKMVIAPIIFFTVVIGIGNMGDLKKIGRIGGKALLYF
EIVTTFALAIGIVVVNLIKPGVGFNTDAAKGGDISQYTKQAEETSHGFVDFVLSIIPDNVVAAMASGELLPVLFFAVLFG
LAAAGLGEKAKPVLSLFERIAEIFFGVVNMVMKVSPIAAFGAMAYTIGTFGLGSLVSLGKLMGSVYITMALFIFIVLGGI
AKFYGFNIFKFLAYIKEELLLVLGTSSSESALPRLMERLEKYGCSKSVVGLVVPTGYSFNLDGTSIYLSMAAIFIAQAYG
IDLSIWQELTLLGVLMLTSKGAAGVTGSGFITLAATLAAFPMIPVEGIALLLGVDRFMSEARAITNIIGNAVATVVVSKM
ENEFHPSAEQKLAEGNMTIAK
>Mature_421_residues
MRGKFKNLTVQVIIGIVLGIIVGFLFPEFGSKLKVLADGFIKLIKMVIAPIIFFTVVIGIGNMGDLKKIGRIGGKALLYF
EIVTTFALAIGIVVVNLIKPGVGFNTDAAKGGDISQYTKQAEETSHGFVDFVLSIIPDNVVAAMASGELLPVLFFAVLFG
LAAAGLGEKAKPVLSLFERIAEIFFGVVNMVMKVSPIAAFGAMAYTIGTFGLGSLVSLGKLMGSVYITMALFIFIVLGGI
AKFYGFNIFKFLAYIKEELLLVLGTSSSESALPRLMERLEKYGCSKSVVGLVVPTGYSFNLDGTSIYLSMAAIFIAQAYG
IDLSIWQELTLLGVLMLTSKGAAGVTGSGFITLAATLAAFPMIPVEGIALLLGVDRFMSEARAITNIIGNAVATVVVSKM
ENEFHPSAEQKLAEGNMTIAK

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI21314632, Length=426, Percent_Identity=26.9953051643192, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI169790839, Length=489, Percent_Identity=25.1533742331288, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI40254478, Length=451, Percent_Identity=26.6075388026608, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI66773030, Length=389, Percent_Identity=26.7352185089974, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI4827012, Length=273, Percent_Identity=28.2051282051282, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI5032093, Length=374, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI223468564, Length=242, Percent_Identity=30.5785123966942, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI223468566, Length=249, Percent_Identity=30.1204819277108, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI262359914, Length=419, Percent_Identity=24.8210023866348, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI194239697, Length=292, Percent_Identity=28.0821917808219, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI301601644, Length=171, Percent_Identity=28.6549707602339, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1789947, Length=402, Percent_Identity=57.4626865671642, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1790514, Length=410, Percent_Identity=44.390243902439, Blast_Score=354, Evalue=6e-99,
Organism=Escherichia coli, GI1788024, Length=400, Percent_Identity=23.25, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17537407, Length=460, Percent_Identity=28.9130434782609, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI71996953, Length=459, Percent_Identity=25.4901960784314, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71983106, Length=466, Percent_Identity=25.7510729613734, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71983099, Length=466, Percent_Identity=25.7510729613734, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI193206505, Length=454, Percent_Identity=24.2290748898678, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI193206654, Length=483, Percent_Identity=22.9813664596273, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17541374, Length=406, Percent_Identity=22.4137931034483, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24583025, Length=463, Percent_Identity=22.8941684665227, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17137668, Length=463, Percent_Identity=22.8941684665227, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24583023, Length=463, Percent_Identity=22.8941684665227, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI281360483, Length=468, Percent_Identity=26.4957264957265, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17137666, Length=468, Percent_Identity=26.4957264957265, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281360481, Length=468, Percent_Identity=26.4957264957265, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 44656; Mature: 44656

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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C
>Mature Secondary Structure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C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA