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The map label for this gene is ldh [H]

Identifier: 138894161

GI number: 138894161

Start: 542632

End: 543585

Strand: Direct

Name: ldh [H]

Synonym: GTNG_0487

Alternate gene names: 138894161

Gene position: 542632-543585 (Clockwise)

Preceding gene: 138894160

Following gene: 138894162

Centisome position: 15.28

GC content: 49.06

Gene sequence:

>954_bases
ATGAAAAACGGAGGAGGAAACAGAGTAGCGGTTGTCGGCACAGGGTTTGTCGGTTCCAGCTATGCATTTGCTTTAATGAA
TCAAGGGATTGCTGATGAGATTGTGCTCATTGATGCAAATGAAAATAAAGCCAAGGGCGATGCGATGGATTTAAACCATG
GGAAAGTGTTTGCACCGAATCCCACCAACATTTGGTATGGGGATTATCATGATTGTCGTGATGCTGATTTAGTCGTCATT
TGCGCCGGTGCCAACCAAAAACCAGGTGAGACGAGGCTCGATCTAGTCGATAAAAATATCGCCATTTTCCGTTCGATTGT
CGAATCGGTTATGGCGTCCGGGTTTCAAGGGTTGTTTCTTGTCGCTACCAATCCGGTCGACATTTTGACCTACGCGACAT
GGAAGTTCAGTGGCTTGCCTTATGAGCGGGTGATCGGTTCGGGGACAATTTTAGATACGGCACGGTTCCGCTTTTTGTTA
GGTGAGTACTTCGATATTGCCCCGACGAATGTACATGCCTACATTATTGGCGAACACGGCGATACTGAGCTTCCGGTCTG
GAGTCAGGCTGATATCGGCGGCGTGCCGATCCGCAAGTTGATCGAATCGAAAGGAGAGCAGGCAAGGGAAGAGCTGGAGC
GCATTTTTGTCAACGTTCGTGATGCCGCTTATCAAATTATCGAGAAAAAAGGGGCGACGTACTACGGCATTGCCATGGGG
CTCGCCCGCGTGACGCGCGCCATTTTGCATAACGAAAACGCTATTTTGACCGTCTCGGCTTACTTAGACGGTTTATATGG
CGAACGTGACGTCTATATCGGTGTACCGGCGGTCATCAACCGTCATGGTATTCGCGAAGTGATTGAGCTTGAGCTAGACG
ATAACGAGCAAAAATGGTTTCAACATAGCGCAGCGACACTGAAAGGTGTGTTGGCTCGCTCCTTTGCGCAGTAA

Upstream 100 bases:

>100_bases
AACGTCCAAACAATTGCCATAATCGTTTACGCATAGTTTCGATTTCATCGCGTAAAATAATTTGTGAATGTATTCACAAT
AATAAGAAGGGAGAATAGTG

Downstream 100 bases:

>100_bases
GTCAAGGCGTTAACTTTCTACAAAAATGGAGAAGGGCTCTGCTAAACGGGTAGTCTAGGCAGGCAGCGCAGCTTGACGCT
GCTGTTTCAAGTACGATCAC

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKNGGGNRVAVVGTGFVGSSYAFALMNQGIADEIVLIDANENKAKGDAMDLNHGKVFAPNPTNIWYGDYHDCRDADLVVI
CAGANQKPGETRLDLVDKNIAIFRSIVESVMASGFQGLFLVATNPVDILTYATWKFSGLPYERVIGSGTILDTARFRFLL
GEYFDIAPTNVHAYIIGEHGDTELPVWSQADIGGVPIRKLIESKGEQAREELERIFVNVRDAAYQIIEKKGATYYGIAMG
LARVTRAILHNENAILTVSAYLDGLYGERDVYIGVPAVINRHGIREVIELELDDNEQKWFQHSAATLKGVLARSFAQ

Sequences:

>Translated_317_residues
MKNGGGNRVAVVGTGFVGSSYAFALMNQGIADEIVLIDANENKAKGDAMDLNHGKVFAPNPTNIWYGDYHDCRDADLVVI
CAGANQKPGETRLDLVDKNIAIFRSIVESVMASGFQGLFLVATNPVDILTYATWKFSGLPYERVIGSGTILDTARFRFLL
GEYFDIAPTNVHAYIIGEHGDTELPVWSQADIGGVPIRKLIESKGEQAREELERIFVNVRDAAYQIIEKKGATYYGIAMG
LARVTRAILHNENAILTVSAYLDGLYGERDVYIGVPAVINRHGIREVIELELDDNEQKWFQHSAATLKGVLARSFAQ
>Mature_317_residues
MKNGGGNRVAVVGTGFVGSSYAFALMNQGIADEIVLIDANENKAKGDAMDLNHGKVFAPNPTNIWYGDYHDCRDADLVVI
CAGANQKPGETRLDLVDKNIAIFRSIVESVMASGFQGLFLVATNPVDILTYATWKFSGLPYERVIGSGTILDTARFRFLL
GEYFDIAPTNVHAYIIGEHGDTELPVWSQADIGGVPIRKLIESKGEQAREELERIFVNVRDAAYQIIEKKGATYYGIAMG
LARVTRAILHNENAILTVSAYLDGLYGERDVYIGVPAVINRHGIREVIELELDDNEQKWFQHSAATLKGVLARSFAQ

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=303, Percent_Identity=41.2541254125413, Blast_Score=257, Evalue=8e-69,
Organism=Homo sapiens, GI221136809, Length=303, Percent_Identity=41.2541254125413, Blast_Score=257, Evalue=8e-69,
Organism=Homo sapiens, GI15082234, Length=303, Percent_Identity=40.2640264026403, Blast_Score=254, Evalue=5e-68,
Organism=Homo sapiens, GI5031857, Length=306, Percent_Identity=38.8888888888889, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI260099723, Length=306, Percent_Identity=38.8888888888889, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI9257228, Length=302, Percent_Identity=39.7350993377483, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI4504973, Length=302, Percent_Identity=39.7350993377483, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI291575128, Length=303, Percent_Identity=38.9438943894389, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4557032, Length=303, Percent_Identity=38.9438943894389, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI260099727, Length=219, Percent_Identity=42.0091324200913, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI260099725, Length=226, Percent_Identity=41.1504424778761, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI207028494, Length=199, Percent_Identity=37.6884422110553, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI103472011, Length=311, Percent_Identity=27.6527331189711, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI103472015, Length=187, Percent_Identity=32.620320855615, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI21735621, Length=318, Percent_Identity=23.5849056603774, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17535107, Length=289, Percent_Identity=40.1384083044983, Blast_Score=230, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI17554310, Length=336, Percent_Identity=25.297619047619, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17136226, Length=310, Percent_Identity=39.6774193548387, Blast_Score=244, Evalue=5e-65,
Organism=Drosophila melanogaster, GI45550422, Length=307, Percent_Identity=30.9446254071661, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24647881, Length=314, Percent_Identity=27.7070063694268, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24663599, Length=323, Percent_Identity=25.077399380805, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 34829; Mature: 34829

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNGGGNRVAVVGTGFVGSSYAFALMNQGIADEIVLIDANENKAKGDAMDLNHGKVFAPN
CCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCEECCCCEEECCC
PTNIWYGDYHDCRDADLVVICAGANQKPGETRLDLVDKNIAIFRSIVESVMASGFQGLFL
CCEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHEECCHHHHHHHHHHHHHHCCCCEEEE
VATNPVDILTYATWKFSGLPYERVIGSGTILDTARFRFLLGEYFDIAPTNVHAYIIGEHG
EECCCEEEEEEEEEEECCCCHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCEEEEEEECCC
DTELPVWSQADIGGVPIRKLIESKGEQAREELERIFVNVRDAAYQIIEKKGATYYGIAMG
CCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
LARVTRAILHNENAILTVSAYLDGLYGERDVYIGVPAVINRHGIREVIELELDDNEQKWF
HHHHHHHHHCCCCEEEEEEHHHCCCCCCCEEEEECHHHHHHCCCCEEEEEEECCCHHHHH
QHSAATLKGVLARSFAQ
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNGGGNRVAVVGTGFVGSSYAFALMNQGIADEIVLIDANENKAKGDAMDLNHGKVFAPN
CCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCEECCCCEEECCC
PTNIWYGDYHDCRDADLVVICAGANQKPGETRLDLVDKNIAIFRSIVESVMASGFQGLFL
CCEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHEECCHHHHHHHHHHHHHHCCCCEEEE
VATNPVDILTYATWKFSGLPYERVIGSGTILDTARFRFLLGEYFDIAPTNVHAYIIGEHG
EECCCEEEEEEEEEEECCCCHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCEEEEEEECCC
DTELPVWSQADIGGVPIRKLIESKGEQAREELERIFVNVRDAAYQIIEKKGATYYGIAMG
CCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
LARVTRAILHNENAILTVSAYLDGLYGERDVYIGVPAVINRHGIREVIELELDDNEQKWF
HHHHHHHHHCCCCEEEEEEHHHCCCCCCCEEEEECHHHHHHCCCCEEEEEEECCCHHHHH
QHSAATLKGVLARSFAQ
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3675869; 3297694 [H]