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The map label for this gene is lutR [H]

Identifier: 138894043

GI number: 138894043

Start: 419305

End: 419967

Strand: Reverse

Name: lutR [H]

Synonym: GTNG_0369

Alternate gene names: 138894043

Gene position: 419967-419305 (Counterclockwise)

Preceding gene: 138894047

Following gene: 138894042

Centisome position: 11.83

GC content: 50.23

Gene sequence:

>663_bases
ATGATTAAAAACGGCGTGCTAAAGCCTGGCGATAAACTCGATTCCGTCCAACAGCTGGCCGAACAGTTTCAAGTCGGACG
GGCCGCCATTCGCGAGGCACTCACTGCTTTGAAAGCGATGGGGCTAATCGAACTCAAACAAGGAGAAGGAACGTACGTAC
GTGAGTTCGATCCGACAATGATGACGTTTCCGTTATCGATTGCCGTCTTAATGAACAAGGAAGATATTTGGCATTTGCTT
GAAGTACGCAAGCTTCTTGAGGCAGGCGCCGCCTCGCTCGCTGCTACGAAGCGGACAGACCGTGATCTTAAAGCGATGAT
GGAAGCCCTTCGGCAAATGAAAGAAGGGATTGGCAGCGATGAGCTTGGCGAGAAAGCAGATTTCGCATTCCATATGGCCA
TTGCGGCCGCGTCACAAAATCCGATGCTCACAAGCATCATGAACAGCGTATCGGGCATGATCGTCGAAACGATGCGAGAA
ACGCGCCGCATTTGGCTGTTTTCGAAACAAACGACGACCGAAAAGCTACTTGCTGAACACCAAGCCATTTTCGAAGCCAT
TCGCGAACAAAATCCAGACGAAGCTCGGACGCGCATGCTTGAGCATTTGACTAATGTCGAAAACGTGTTGTATCGCTACA
TTCGCACTCAGCAGTCAAACTGA

Upstream 100 bases:

>100_bases
AATCGGGAATCTACTTTATCGTATTGAGGTGGATCCATTGATGTTTAAGCAGATTAAAACAAAAAAAATTTATGAAGAAG
TGGCCGAAGCTATTTTCGAC

Downstream 100 bases:

>100_bases
GTGCATTTTTTACAAAAAAGAACTACAAATCTGAAAAAATCATTTATAATAGACATATAGTCATCAGATGACCTGTTCCC
TCTGCTAGATAAAATAGGAG

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MIKNGVLKPGDKLDSVQQLAEQFQVGRAAIREALTALKAMGLIELKQGEGTYVREFDPTMMTFPLSIAVLMNKEDIWHLL
EVRKLLEAGAASLAATKRTDRDLKAMMEALRQMKEGIGSDELGEKADFAFHMAIAAASQNPMLTSIMNSVSGMIVETMRE
TRRIWLFSKQTTTEKLLAEHQAIFEAIREQNPDEARTRMLEHLTNVENVLYRYIRTQQSN

Sequences:

>Translated_220_residues
MIKNGVLKPGDKLDSVQQLAEQFQVGRAAIREALTALKAMGLIELKQGEGTYVREFDPTMMTFPLSIAVLMNKEDIWHLL
EVRKLLEAGAASLAATKRTDRDLKAMMEALRQMKEGIGSDELGEKADFAFHMAIAAASQNPMLTSIMNSVSGMIVETMRE
TRRIWLFSKQTTTEKLLAEHQAIFEAIREQNPDEARTRMLEHLTNVENVLYRYIRTQQSN
>Mature_220_residues
MIKNGVLKPGDKLDSVQQLAEQFQVGRAAIREALTALKAMGLIELKQGEGTYVREFDPTMMTFPLSIAVLMNKEDIWHLL
EVRKLLEAGAASLAATKRTDRDLKAMMEALRQMKEGIGSDELGEKADFAFHMAIAAASQNPMLTSIMNSVSGMIVETMRE
TRRIWLFSKQTTTEKLLAEHQAIFEAIREQNPDEARTRMLEHLTNVENVLYRYIRTQQSN

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786303, Length=216, Percent_Identity=31.0185185185185, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1790780, Length=224, Percent_Identity=29.4642857142857, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1789353, Length=216, Percent_Identity=30.0925925925926, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790032, Length=225, Percent_Identity=30.2222222222222, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI48994955, Length=203, Percent_Identity=30.5418719211823, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789621, Length=228, Percent_Identity=27.1929824561404, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI87082208, Length=214, Percent_Identity=27.5700934579439, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24887; Mature: 24887

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNGVLKPGDKLDSVQQLAEQFQVGRAAIREALTALKAMGLIELKQGEGTYVREFDPTM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCHH
MTFPLSIAVLMNKEDIWHLLEVRKLLEAGAASLAATKRTDRDLKAMMEALRQMKEGIGSD
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCC
ELGEKADFAFHMAIAAASQNPMLTSIMNSVSGMIVETMRETRRIWLFSKQTTTEKLLAEH
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHH
QAIFEAIREQNPDEARTRMLEHLTNVENVLYRYIRTQQSN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKNGVLKPGDKLDSVQQLAEQFQVGRAAIREALTALKAMGLIELKQGEGTYVREFDPTM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCHH
MTFPLSIAVLMNKEDIWHLLEVRKLLEAGAASLAATKRTDRDLKAMMEALRQMKEGIGSD
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCC
ELGEKADFAFHMAIAAASQNPMLTSIMNSVSGMIVETMRETRRIWLFSKQTTTEKLLAEH
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHH
QAIFEAIREQNPDEARTRMLEHLTNVENVLYRYIRTQQSN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]