Click here to switch to the map view.

The map label for this gene is ligA [H]

Identifier: 138893932

GI number: 138893932

Start: 286088

End: 288100

Strand: Direct

Name: ligA [H]

Synonym: GTNG_0256

Alternate gene names: 138893932

Gene position: 286088-288100 (Clockwise)

Preceding gene: 138893931

Following gene: 138893933

Centisome position: 8.06

GC content: 55.79

Gene sequence:

>2013_bases
ATGGACCGCCAACAAGCCAAACGGCGCGCGGCCGAGCTGCGCGAGCTGTTAAACCGCTACGGGTATGAATATTATGTGCT
CGACCGGCCGTCCGTCCCGGATGCGGAGTATGACCGGCTTATGCAAGAGCTCATCGCTATTGAAAAACAGTATCCGGAGC
TAAAAACGAGCGATTCGCCGACTCAGCGAATCGGCGGCCCGCCGCTTGAGGCGTTTCGCAAGGTGACGCATCGCGTCCCG
ATGATGAGCTTAGCAAACGCGTTTAACGAAGGCGATTTGCGTGATTTTGACCGCCGCGTTCGTCAAGAAGTGGGAGAGGC
GGCGTATGTGTGTGAATTGAAAATCGACGGTCTTGCCGTCTCAGTTCGTTATGAAGACGGCTATTTCGTCCAAGGAGCGA
CGCGTGGCGACGGAACGACAGGGGAGGACATTACAGAAAATTTGAAGACGATCCGCTCGCTGCCGCTGCGCCTCAACGAG
CCGGTGTCGCTGGAGGCGCGCGGCGAGGCGTTCATGCCGAAAGCGTCATTTTTACGCTTGAATGAAGAGCGCCAAGCGCG
TGGTGAAGAACTGTTTGCCAATCCGCGCAACGCCGCGGCCGGTTCGCTCCGCCAGCTCGATCCGAAAGTGGCAGCTTCGC
GCCAGCTCGATCTGTTTGTCTACGGCTTAGCCAATGCTGAGGAGCTGGGCATTGAGTCACATAGCGCGGCGCTCAGCTAT
TTGCAGTCGCTCGGGTTCAAAGTGAACCCGGAGCGACGGCGCTGTGCCACGATTGATGAAGTGATCGCATTTGTCAACGA
GTGGAAAGAGAAGCGGCCGCAACTGCCGTATGAGATCGACGGCATCGTCATTAAAGTCGATTCGTTTGCCCAACAGCGGC
AGCTCGGGGCGACAGCGAAAAGCCCACGTTGGGCGATCGCCTACAAATTCCCAGCCGAAGAAGTGGTCACCACGCTCATC
GGCATCGAGGTGAATGTGGGTCGCACCGGCGCTGTCACTCCGACGGCAATCTTAGAGCCGGTTCGTGTCGCCGGCACGAC
CGTGCAGCGCGCCACTCTTCATAACGAAGATTTTATTCGTGAGAAAGATATTCGCATCGGCGATGCCGTCATTATTAAAA
AGGCGGGCGATATCATCCCGGAAGTCGTCGGTGTCGTCGTCGATCGGCGCGACGGGGACGAAGTGCCGTTTACAATGCCG
ACGCATTGTCCGGAATGTGAAAGCGAACTCGTTCGTCTTGACGGGGAAGTGGCGCTCCGTTGCTTAAACCCGAAATGCCC
GGCTCAGCTGCGTGAACGGCTCATTCACTTTGCGTCAAGGTCGGCAATGAACATAGAAGGGTTAGGAGAAAAAGTTGTTA
CCCAACTGTTTAACGCTGGCCTTGTCCATGATGTCGCCGATTTGTACCAGTTGACGAAAGAACAGCTTGTCGGGCTAGAA
CGGATGGGTGAAAAGTCGGCAGCGAATTTGCTTGCAGCGATTGAAGCGTCAAAGCAAAACTCGCTTGAGCGGCTGTTATT
CGGCCTTGGTATCCGTTATGTCGGGGCGAAGGCGGCCCAGCTGTTGGCCGAACATTTCGAAACGATGGAGCGTCTCGAGG
CAGCGACAAAAGACGAATTGATGGCCGTGCCGGAGATCGGGGAAAAAATGGCCGACTCGATTACCACTTATTTTTCCCAG
CCGGAAGCGGTTGAGTTGTTGAATGAGCTGCGCACCTATGGTGTCAATATGGCATACAAAGGACGGAAACGGACGGCTGA
AACGCCAGCCTCATCGGTGCTGGCCGGCAAAACGGTCGTCCTGACCGGCAAACTTGCGTCGATGTCACGAAATGAAGCGA
AAGAACAAATCGAGCGGCTCGGCGGTCGTGTCACCGGCAGCGTCAGCCGCAGCACCGACATCGTCATCGCCGGTGAAGAT
GCCGGTTCAAAGCTCGATAAAGCTCAGCAGCTTGGCATTGAGATTTGGGATGAAACACGATTTTTGCAAGAGATAAGCAG
GGAGGAACAATGA

Upstream 100 bases:

>100_bases
AGAACTCGATATCGCTTTTCCGAGTCCGATCGGAATTAAGCGGCTGCTCGCTAAATTTGCGCCGATTGAGAAAGTGTAAG
AAAGGAGAACGAGTGAACGT

Downstream 100 bases:

>100_bases
AACGAATCTCGATCATTAGCAGCTTGGTTGCCTGCCGTTGGATAAAGCGGGCCCGAAAGCGGCTCGCCGTCCTCGGGCTC
GCCTCCTTCTGTTTGTTGTC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MDRQQAKRRAAELRELLNRYGYEYYVLDRPSVPDAEYDRLMQELIAIEKQYPELKTSDSPTQRIGGPPLEAFRKVTHRVP
MMSLANAFNEGDLRDFDRRVRQEVGEAAYVCELKIDGLAVSVRYEDGYFVQGATRGDGTTGEDITENLKTIRSLPLRLNE
PVSLEARGEAFMPKASFLRLNEERQARGEELFANPRNAAAGSLRQLDPKVAASRQLDLFVYGLANAEELGIESHSAALSY
LQSLGFKVNPERRRCATIDEVIAFVNEWKEKRPQLPYEIDGIVIKVDSFAQQRQLGATAKSPRWAIAYKFPAEEVVTTLI
GIEVNVGRTGAVTPTAILEPVRVAGTTVQRATLHNEDFIREKDIRIGDAVIIKKAGDIIPEVVGVVVDRRDGDEVPFTMP
THCPECESELVRLDGEVALRCLNPKCPAQLRERLIHFASRSAMNIEGLGEKVVTQLFNAGLVHDVADLYQLTKEQLVGLE
RMGEKSAANLLAAIEASKQNSLERLLFGLGIRYVGAKAAQLLAEHFETMERLEAATKDELMAVPEIGEKMADSITTYFSQ
PEAVELLNELRTYGVNMAYKGRKRTAETPASSVLAGKTVVLTGKLASMSRNEAKEQIERLGGRVTGSVSRSTDIVIAGED
AGSKLDKAQQLGIEIWDETRFLQEISREEQ

Sequences:

>Translated_670_residues
MDRQQAKRRAAELRELLNRYGYEYYVLDRPSVPDAEYDRLMQELIAIEKQYPELKTSDSPTQRIGGPPLEAFRKVTHRVP
MMSLANAFNEGDLRDFDRRVRQEVGEAAYVCELKIDGLAVSVRYEDGYFVQGATRGDGTTGEDITENLKTIRSLPLRLNE
PVSLEARGEAFMPKASFLRLNEERQARGEELFANPRNAAAGSLRQLDPKVAASRQLDLFVYGLANAEELGIESHSAALSY
LQSLGFKVNPERRRCATIDEVIAFVNEWKEKRPQLPYEIDGIVIKVDSFAQQRQLGATAKSPRWAIAYKFPAEEVVTTLI
GIEVNVGRTGAVTPTAILEPVRVAGTTVQRATLHNEDFIREKDIRIGDAVIIKKAGDIIPEVVGVVVDRRDGDEVPFTMP
THCPECESELVRLDGEVALRCLNPKCPAQLRERLIHFASRSAMNIEGLGEKVVTQLFNAGLVHDVADLYQLTKEQLVGLE
RMGEKSAANLLAAIEASKQNSLERLLFGLGIRYVGAKAAQLLAEHFETMERLEAATKDELMAVPEIGEKMADSITTYFSQ
PEAVELLNELRTYGVNMAYKGRKRTAETPASSVLAGKTVVLTGKLASMSRNEAKEQIERLGGRVTGSVSRSTDIVIAGED
AGSKLDKAQQLGIEIWDETRFLQEISREEQ
>Mature_670_residues
MDRQQAKRRAAELRELLNRYGYEYYVLDRPSVPDAEYDRLMQELIAIEKQYPELKTSDSPTQRIGGPPLEAFRKVTHRVP
MMSLANAFNEGDLRDFDRRVRQEVGEAAYVCELKIDGLAVSVRYEDGYFVQGATRGDGTTGEDITENLKTIRSLPLRLNE
PVSLEARGEAFMPKASFLRLNEERQARGEELFANPRNAAAGSLRQLDPKVAASRQLDLFVYGLANAEELGIESHSAALSY
LQSLGFKVNPERRRCATIDEVIAFVNEWKEKRPQLPYEIDGIVIKVDSFAQQRQLGATAKSPRWAIAYKFPAEEVVTTLI
GIEVNVGRTGAVTPTAILEPVRVAGTTVQRATLHNEDFIREKDIRIGDAVIIKKAGDIIPEVVGVVVDRRDGDEVPFTMP
THCPECESELVRLDGEVALRCLNPKCPAQLRERLIHFASRSAMNIEGLGEKVVTQLFNAGLVHDVADLYQLTKEQLVGLE
RMGEKSAANLLAAIEASKQNSLERLLFGLGIRYVGAKAAQLLAEHFETMERLEAATKDELMAVPEIGEKMADSITTYFSQ
PEAVELLNELRTYGVNMAYKGRKRTAETPASSVLAGKTVVLTGKLASMSRNEAKEQIERLGGRVTGSVSRSTDIVIAGED
AGSKLDKAQQLGIEIWDETRFLQEISREEQ

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=77, Percent_Identity=49.3506493506493, Blast_Score=67, Evalue=7e-11,
Organism=Escherichia coli, GI1788750, Length=661, Percent_Identity=49.4704992435704, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=511, Percent_Identity=23.679060665362, Blast_Score=123, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74526; Mature: 74526

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRQQAKRRAAELRELLNRYGYEYYVLDRPSVPDAEYDRLMQELIAIEKQYPELKTSDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
TQRIGGPPLEAFRKVTHRVPMMSLANAFNEGDLRDFDRRVRQEVGEAAYVCELKIDGLAV
HHHCCCCCHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEEEECCEEE
SVRYEDGYFVQGATRGDGTTGEDITENLKTIRSLPLRLNEPVSLEARGEAFMPKASFLRL
EEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCHHHHEE
NEERQARGEELFANPRNAAAGSLRQLDPKVAASRQLDLFVYGLANAEELGIESHSAALSY
CHHHHHCCHHHHCCCCCHHHCCHHHCCHHHHCCCCEEEEEEECCCHHHHCCCHHHHHHHH
LQSLGFKVNPERRRCATIDEVIAFVNEWKEKRPQLPYEIDGIVIKVDSFAQQRQLGATAK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECHHHHHHHHCCCCC
SPRWAIAYKFPAEEVVTTLIGIEVNVGRTGAVTPTAILEPVRVAGTTVQRATLHNEDFIR
CCCEEEEEECCHHHHHHHHHCEEEECCCCCCCCHHHHHCCHHHHCCHHHHHHHCCCHHHH
EKDIRIGDAVIIKKAGDIIPEVVGVVVDRRDGDEVPFTMPTHCPECESELVRLDGEVALR
HCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHCCCHHEEE
CLNPKCPAQLRERLIHFASRSAMNIEGLGEKVVTQLFNAGLVHDVADLYQLTKEQLVGLE
ECCCCCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RMGEKSAANLLAAIEASKQNSLERLLFGLGIRYVGAKAAQLLAEHFETMERLEAATKDEL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
MAVPEIGEKMADSITTYFSQPEAVELLNELRTYGVNMAYKGRKRTAETPASSVLAGKTVV
EECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHCCCEEE
LTGKLASMSRNEAKEQIERLGGRVTGSVSRSTDIVIAGEDAGSKLDKAQQLGIEIWDETR
EECCHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHH
FLQEISREEQ
HHHHHHHCCC
>Mature Secondary Structure
MDRQQAKRRAAELRELLNRYGYEYYVLDRPSVPDAEYDRLMQELIAIEKQYPELKTSDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
TQRIGGPPLEAFRKVTHRVPMMSLANAFNEGDLRDFDRRVRQEVGEAAYVCELKIDGLAV
HHHCCCCCHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEEEECCEEE
SVRYEDGYFVQGATRGDGTTGEDITENLKTIRSLPLRLNEPVSLEARGEAFMPKASFLRL
EEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCHHHHEE
NEERQARGEELFANPRNAAAGSLRQLDPKVAASRQLDLFVYGLANAEELGIESHSAALSY
CHHHHHCCHHHHCCCCCHHHCCHHHCCHHHHCCCCEEEEEEECCCHHHHCCCHHHHHHHH
LQSLGFKVNPERRRCATIDEVIAFVNEWKEKRPQLPYEIDGIVIKVDSFAQQRQLGATAK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECHHHHHHHHCCCCC
SPRWAIAYKFPAEEVVTTLIGIEVNVGRTGAVTPTAILEPVRVAGTTVQRATLHNEDFIR
CCCEEEEEECCHHHHHHHHHCEEEECCCCCCCCHHHHHCCHHHHCCHHHHHHHCCCHHHH
EKDIRIGDAVIIKKAGDIIPEVVGVVVDRRDGDEVPFTMPTHCPECESELVRLDGEVALR
HCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHCCCHHEEE
CLNPKCPAQLRERLIHFASRSAMNIEGLGEKVVTQLFNAGLVHDVADLYQLTKEQLVGLE
ECCCCCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RMGEKSAANLLAAIEASKQNSLERLLFGLGIRYVGAKAAQLLAEHFETMERLEAATKDEL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
MAVPEIGEKMADSITTYFSQPEAVELLNELRTYGVNMAYKGRKRTAETPASSVLAGKTVV
EECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHCCCEEE
LTGKLASMSRNEAKEQIERLGGRVTGSVSRSTDIVIAGEDAGSKLDKAQQLGIEIWDETR
EECCHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHH
FLQEISREEQ
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA