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The map label for this gene is murF [H]

Identifier: 138893875

GI number: 138893875

Start: 212831

End: 214213

Strand: Direct

Name: murF [H]

Synonym: GTNG_0199

Alternate gene names: 138893875

Gene position: 212831-214213 (Clockwise)

Preceding gene: 138893874

Following gene: 138893876

Centisome position: 5.99

GC content: 54.45

Gene sequence:

>1383_bases
ATGATGAAACGGACGGTGCAGCAAATTGCTGAGATGACCGGCGGCCGGTACGCGAATCCGGAGTGGGGGAATATTGCTGT
CAGCGGCGTATCAACTGACACGAGGACGATCCAAGCTGGCAACTTGTACGTTCCGCTGCTTGGCGAGACGTTTAACGGTC
ACGCTTTCGTGGAGGAAGCGGCGCAAAAAGGAGCAGCAGCTGTGCTGTGGGCCGAAGCGGAAGGGACACCGCCAGAAAGT
GTTCCGGCGATTGTCGTCGATGATACGCTTTCGGCACTGCAGCGTCTCGCCACCCGCTACCGTGAACAGCTCGGGCTGAA
GGTTGTTGGTGTGACTGGCAGCAATGGCAAAACGACGACGAAAGATATGATCGCTGCGCTTCTTTCATCCCAATATCGCG
TGCAAAAAACAGAAGGAAACTTAAACAACCATATCGGCGTTCCGCTCACGCTCCTTGCGCTCGAGGAGGGGACAGACATC
GCCGTTGTGGAAATGGGGATGAGTGGGTTTGGTGAAATTGAGCAGCTCACGACGATCGCACGGCCGAATGCTGCCGTCAT
CACCAACATCGGTGAAGCACATTTGCAAGAGCTCGGGTCACGGGAAGGCATCGCCAAGGCAAAGCTAGAAATTTTATCTG
GATTGCAAGGCGACGGTTTGTTCGTCTATCACGGCGATGAGCCACTTTTGGCTGAGCTCGTGCCAAGCTTGCCGCTTCCT
CAGCACGTTGTGACGTTTGGGGAAGGTGAGGACAACGATTATTATCCGACAACTGTGCGCATTGAAGCAGCGGGCACGTC
GTTTGTCATCAACGAGCTCCCTCGCCAGACGCTATTTATTCCAGTTTTAGGGAAACACCATGTATACAACGCGCTGGCCG
CCATCGCTGTCGCCCGCTTTTTCGGCGTCAGCTGGGAGAACATTGCTGCCGCTCTCTCCCGCTTGCAAGTGACGCGCATG
CGTACGGAAGTCGTTAAAACGAAGCGCGGCTTTACAGTGATTAATGATGCCTACAACGCCAGCCCGACATCGATGCGCGC
TGCTTTAATGCTGCTCACGGAATTGGACGGCTATCGGAAGAAAATCGCTGTATTGGGCGATATGTTGGAGCTTGGTGACA
AAGAGGTCGAGTTCCACCAAGCGATTGGGGAGATGCTGCGGCCAGAGATGGTAGATTACGTGTTTACTTACGGACCACTG
GCGCGTCATATCGCTACGGCCGCCACGCCGTTTTTTGATCAAGGCCAAGTGAAGGCGTTTGACGATAAAGCGGCATTGGC
CGAGGCGGTGCTTTCCGTCGCAACCCCAGACGATATCGTTTTATTGAAGGCCTCGCGTGGTATGAGGTTGGAAGAGTTAC
TCGGTTATTGGGGGGAAAATTAG

Upstream 100 bases:

>100_bases
TCGGAATTGATCGAACGCCTTATTGCATTGGCATTGGAACGACATCAAGAAAAGCAGATGATTACGTACACATTTGAAAA
GTAAAAGAGGGTGAGAGCGG

Downstream 100 bases:

>100_bases
CAAAAGCGAGAAAAACGAAAAGCGGCGCTATGGGCGCCGCTTTTCGATCAGGCCGGGGGGTGGGATCATGATCGGCTGTT
TATGCATTCACGGATTTACC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MMKRTVQQIAEMTGGRYANPEWGNIAVSGVSTDTRTIQAGNLYVPLLGETFNGHAFVEEAAQKGAAAVLWAEAEGTPPES
VPAIVVDDTLSALQRLATRYREQLGLKVVGVTGSNGKTTTKDMIAALLSSQYRVQKTEGNLNNHIGVPLTLLALEEGTDI
AVVEMGMSGFGEIEQLTTIARPNAAVITNIGEAHLQELGSREGIAKAKLEILSGLQGDGLFVYHGDEPLLAELVPSLPLP
QHVVTFGEGEDNDYYPTTVRIEAAGTSFVINELPRQTLFIPVLGKHHVYNALAAIAVARFFGVSWENIAAALSRLQVTRM
RTEVVKTKRGFTVINDAYNASPTSMRAALMLLTELDGYRKKIAVLGDMLELGDKEVEFHQAIGEMLRPEMVDYVFTYGPL
ARHIATAATPFFDQGQVKAFDDKAALAEAVLSVATPDDIVLLKASRGMRLEELLGYWGEN

Sequences:

>Translated_460_residues
MMKRTVQQIAEMTGGRYANPEWGNIAVSGVSTDTRTIQAGNLYVPLLGETFNGHAFVEEAAQKGAAAVLWAEAEGTPPES
VPAIVVDDTLSALQRLATRYREQLGLKVVGVTGSNGKTTTKDMIAALLSSQYRVQKTEGNLNNHIGVPLTLLALEEGTDI
AVVEMGMSGFGEIEQLTTIARPNAAVITNIGEAHLQELGSREGIAKAKLEILSGLQGDGLFVYHGDEPLLAELVPSLPLP
QHVVTFGEGEDNDYYPTTVRIEAAGTSFVINELPRQTLFIPVLGKHHVYNALAAIAVARFFGVSWENIAAALSRLQVTRM
RTEVVKTKRGFTVINDAYNASPTSMRAALMLLTELDGYRKKIAVLGDMLELGDKEVEFHQAIGEMLRPEMVDYVFTYGPL
ARHIATAATPFFDQGQVKAFDDKAALAEAVLSVATPDDIVLLKASRGMRLEELLGYWGEN
>Mature_460_residues
MMKRTVQQIAEMTGGRYANPEWGNIAVSGVSTDTRTIQAGNLYVPLLGETFNGHAFVEEAAQKGAAAVLWAEAEGTPPES
VPAIVVDDTLSALQRLATRYREQLGLKVVGVTGSNGKTTTKDMIAALLSSQYRVQKTEGNLNNHIGVPLTLLALEEGTDI
AVVEMGMSGFGEIEQLTTIARPNAAVITNIGEAHLQELGSREGIAKAKLEILSGLQGDGLFVYHGDEPLLAELVPSLPLP
QHVVTFGEGEDNDYYPTTVRIEAAGTSFVINELPRQTLFIPVLGKHHVYNALAAIAVARFFGVSWENIAAALSRLQVTRM
RTEVVKTKRGFTVINDAYNASPTSMRAALMLLTELDGYRKKIAVLGDMLELGDKEVEFHQAIGEMLRPEMVDYVFTYGPL
ARHIATAATPFFDQGQVKAFDDKAALAEAVLSVATPDDIVLLKASRGMRLEELLGYWGEN

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=466, Percent_Identity=34.9785407725322, Blast_Score=210, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49813; Mature: 49813

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRTVQQIAEMTGGRYANPEWGNIAVSGVSTDTRTIQAGNLYVPLLGETFNGHAFVEEA
CCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCEEEEEECCCCCCHHHHHHH
AQKGAAAVLWAEAEGTPPESVPAIVVDDTLSALQRLATRYREQLGLKVVGVTGSNGKTTT
HHCCCEEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHH
KDMIAALLSSQYRVQKTEGNLNNHIGVPLTLLALEEGTDIAVVEMGMSGFGEIEQLTTIA
HHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHC
RPNAAVITNIGEAHLQELGSREGIAKAKLEILSGLQGDGLFVYHGDEPLLAELVPSLPLP
CCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCCC
QHVVTFGEGEDNDYYPTTVRIEAAGTSFVINELPRQTLFIPVLGKHHVYNALAAIAVARF
HHEEEECCCCCCCCCCEEEEEEECCCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHH
FGVSWENIAAALSRLQVTRMRTEVVKTKRGFTVINDAYNASPTSMRAALMLLTELDGYRK
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
KIAVLGDMLELGDKEVEFHQAIGEMLRPEMVDYVFTYGPLARHIATAATPFFDQGQVKAF
HHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
DDKAALAEAVLSVATPDDIVLLKASRGMRLEELLGYWGEN
CHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MMKRTVQQIAEMTGGRYANPEWGNIAVSGVSTDTRTIQAGNLYVPLLGETFNGHAFVEEA
CCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCEEEEEECCCCCCHHHHHHH
AQKGAAAVLWAEAEGTPPESVPAIVVDDTLSALQRLATRYREQLGLKVVGVTGSNGKTTT
HHCCCEEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHH
KDMIAALLSSQYRVQKTEGNLNNHIGVPLTLLALEEGTDIAVVEMGMSGFGEIEQLTTIA
HHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHC
RPNAAVITNIGEAHLQELGSREGIAKAKLEILSGLQGDGLFVYHGDEPLLAELVPSLPLP
CCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCCC
QHVVTFGEGEDNDYYPTTVRIEAAGTSFVINELPRQTLFIPVLGKHHVYNALAAIAVARF
HHEEEECCCCCCCCCCEEEEEEECCCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHH
FGVSWENIAAALSRLQVTRMRTEVVKTKRGFTVINDAYNASPTSMRAALMLLTELDGYRK
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
KIAVLGDMLELGDKEVEFHQAIGEMLRPEMVDYVFTYGPLARHIATAATPFFDQGQVKAF
HHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
DDKAALAEAVLSVATPDDIVLLKASRGMRLEELLGYWGEN
CHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]