Click here to switch to the map view.

The map label for this gene is rocR [H]

Identifier: 138893843

GI number: 138893843

Start: 177937

End: 179310

Strand: Reverse

Name: rocR [H]

Synonym: GTNG_0167

Alternate gene names: 138893843

Gene position: 179310-177937 (Counterclockwise)

Preceding gene: 138893848

Following gene: 138893840

Centisome position: 5.05

GC content: 50.07

Gene sequence:

>1374_bases
GTGAACCGGGAGATGGAAACGCTTTTATCCATGTATGAGCAAATTCTCGAAATGATCGACCTTGGTGTTCATGCGGTCGA
CCAACAAGGAAAAACAGTCATTTACAACCGAAAAATGCGGGACATTGAGGGAATGGACGTCGAGGATGTACTTGATAAGA
ACATTTTAGACGTCTTCCGCTTCCATCCGGAACAGCCGAGCACATTGCTTGAAGCGCTGCGCACCGGTGAAAGCATATTG
AACAAGCAACAAACATATTTCAACAACAAAGGGCAAGCAGTGACAACGATCAATCAAACACATCCATTATGGAAAGACGG
ATGCATCATCGGAGCGGTGGAAATCGCCAAAGACGTCACGAAATTCCGCAAGCTGATTGCCGAGCAGCACCAACGCGGAG
AACTAAGCCGCTCACTGGCAGGCATCATCGTCCAAAGCGAAGCGATGCAAGCTGCGATTCGCCTAGCCCGCCATGCCGCC
CGCTCGGAAGCCCCGGTACTGCTTATGGGTGAAAAAGGGACAGGAAAAGATTTGCTTGCTTCTTGCATTCATTACGAAAG
TGAACGGCGCTTGAAACCATTTTTGGCGCAAACGTGTTTGTCGCTCCCGGACGATTGGATGGAAACGATGCTGTTTGGCA
GCGAAGAAAACGGCGAGATCCAGCCTGGTTTGTTCGAACAAGCGGATGGTGGCACTGTGCTACTCGATGATGTCGATTCG
TTAAGCCTGCCGCTCCAACAAAAGCTCGTGCATTTCCTTCAGGAAAAACAGTTTACCCGCGTCCATGGCCATAAGCCCGT
TCGTGCAGACGTCCGCCTTATCACCTCGACAAGCGGTGACCCGATTGACGCCGTCCAGACAGGAGAGTTGCTAAAATCGC
TTTACTACCAGCTCGCGGTGCACTGTATCGTTTTGCCTCCATTGCGCGAGAGAAGAGAGGACATTTTGCCATTGGCCGAC
CATTTCATCCGCCAAGGCAATGAACGCTATGGCTTAAACGTGGAAGGACTTGCGGATGACGTGCAAGAGGCGTTTCTCGC
TTACGACTGGCCTGGCAACGTGCGCGAGCTAGAAACGGTGATCGATGAAACGTTGGAGATGATGGACGAGGGAGAAACGA
TCACATTCGATCATCTTCCAGCTTCGTTCCGCATGAAACTTGCACCCGATGCACCGAAAACAGACTTTTTGTTTAATACA
GAAGACATTCTGCCACTCGAAAAATATATGGAGGAAGTCGAAATTTACTACATCCGCAAAGCGCTGCAACACCACGGCTT
CAACATCACGAAAACCGCTAAAGCGCTTGGCTTAAGCCGGCAAAACTTGCAATACCGCATTCGCAAGTATCGGATTGATA
AGGAATGGGGATGA

Upstream 100 bases:

>100_bases
TTGGCAAAAATTTTTTTCGATCTCCTTCTTCCCCTCCCTTGTCATTCCTCTCTTCGCTAGCTACTTGTGTTATAATACAA
GGAAAGGAGAGGTGCTGATC

Downstream 100 bases:

>100_bases
ACAGTAGGACAACGGAAAAAGAAACGGACGTCCAACAAAAAGCACACGTTCGGAGTGATTCCCATCTTTTCCGTAACGTG
TGCTCCAAAGTAAATTTCTA

Product: NifA/NtrC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MNREMETLLSMYEQILEMIDLGVHAVDQQGKTVIYNRKMRDIEGMDVEDVLDKNILDVFRFHPEQPSTLLEALRTGESIL
NKQQTYFNNKGQAVTTINQTHPLWKDGCIIGAVEIAKDVTKFRKLIAEQHQRGELSRSLAGIIVQSEAMQAAIRLARHAA
RSEAPVLLMGEKGTGKDLLASCIHYESERRLKPFLAQTCLSLPDDWMETMLFGSEENGEIQPGLFEQADGGTVLLDDVDS
LSLPLQQKLVHFLQEKQFTRVHGHKPVRADVRLITSTSGDPIDAVQTGELLKSLYYQLAVHCIVLPPLRERREDILPLAD
HFIRQGNERYGLNVEGLADDVQEAFLAYDWPGNVRELETVIDETLEMMDEGETITFDHLPASFRMKLAPDAPKTDFLFNT
EDILPLEKYMEEVEIYYIRKALQHHGFNITKTAKALGLSRQNLQYRIRKYRIDKEWG

Sequences:

>Translated_457_residues
MNREMETLLSMYEQILEMIDLGVHAVDQQGKTVIYNRKMRDIEGMDVEDVLDKNILDVFRFHPEQPSTLLEALRTGESIL
NKQQTYFNNKGQAVTTINQTHPLWKDGCIIGAVEIAKDVTKFRKLIAEQHQRGELSRSLAGIIVQSEAMQAAIRLARHAA
RSEAPVLLMGEKGTGKDLLASCIHYESERRLKPFLAQTCLSLPDDWMETMLFGSEENGEIQPGLFEQADGGTVLLDDVDS
LSLPLQQKLVHFLQEKQFTRVHGHKPVRADVRLITSTSGDPIDAVQTGELLKSLYYQLAVHCIVLPPLRERREDILPLAD
HFIRQGNERYGLNVEGLADDVQEAFLAYDWPGNVRELETVIDETLEMMDEGETITFDHLPASFRMKLAPDAPKTDFLFNT
EDILPLEKYMEEVEIYYIRKALQHHGFNITKTAKALGLSRQNLQYRIRKYRIDKEWG
>Mature_457_residues
MNREMETLLSMYEQILEMIDLGVHAVDQQGKTVIYNRKMRDIEGMDVEDVLDKNILDVFRFHPEQPSTLLEALRTGESIL
NKQQTYFNNKGQAVTTINQTHPLWKDGCIIGAVEIAKDVTKFRKLIAEQHQRGELSRSLAGIIVQSEAMQAAIRLARHAA
RSEAPVLLMGEKGTGKDLLASCIHYESERRLKPFLAQTCLSLPDDWMETMLFGSEENGEIQPGLFEQADGGTVLLDDVDS
LSLPLQQKLVHFLQEKQFTRVHGHKPVRADVRLITSTSGDPIDAVQTGELLKSLYYQLAVHCIVLPPLRERREDILPLAD
HFIRQGNERYGLNVEGLADDVQEAFLAYDWPGNVRELETVIDETLEMMDEGETITFDHLPASFRMKLAPDAPKTDFLFNT
EDILPLEKYMEEVEIYYIRKALQHHGFNITKTAKALGLSRQNLQYRIRKYRIDKEWG

Specific function: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=357, Percent_Identity=35.2941176470588, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1790437, Length=314, Percent_Identity=35.9872611464968, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1790299, Length=341, Percent_Identity=33.4310850439883, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1787583, Length=350, Percent_Identity=32.2857142857143, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1788905, Length=319, Percent_Identity=32.2884012539185, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1789233, Length=323, Percent_Identity=31.8885448916409, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI87082117, Length=276, Percent_Identity=35.5072463768116, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87081872, Length=302, Percent_Identity=31.4569536423841, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1789087, Length=334, Percent_Identity=33.5329341317365, Blast_Score=158, Evalue=9e-40,
Organism=Escherichia coli, GI1786524, Length=316, Percent_Identity=33.8607594936709, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI87081858, Length=449, Percent_Identity=27.1714922048998, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI87082152, Length=207, Percent_Identity=39.6135265700483, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789828, Length=233, Percent_Identity=30.0429184549356, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF08448 PAS_4; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52205; Mature: 52205

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50112 PAS ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREMETLLSMYEQILEMIDLGVHAVDQQGKTVIYNRKMRDIEGMDVEDVLDKNILDVFR
CCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
FHPEQPSTLLEALRTGESILNKQQTYFNNKGQAVTTINQTHPLWKDGCIIGAVEIAKDVT
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHH
KFRKLIAEQHQRGELSRSLAGIIVQSEAMQAAIRLARHAARSEAPVLLMGEKGTGKDLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHH
SCIHYESERRLKPFLAQTCLSLPDDWMETMLFGSEENGEIQPGLFEQADGGTVLLDDVDS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCC
LSLPLQQKLVHFLQEKQFTRVHGHKPVRADVRLITSTSGDPIDAVQTGELLKSLYYQLAV
CCCCHHHHHHHHHHHHHHHHHCCCCCCEECEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
HCIVLPPLRERREDILPLADHFIRQGNERYGLNVEGLADDVQEAFLAYDWPGNVRELETV
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHH
IDETLEMMDEGETITFDHLPASFRMKLAPDAPKTDFLFNTEDILPLEKYMEEVEIYYIRK
HHHHHHHHCCCCEEEEECCCCHHEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHH
ALQHHGFNITKTAKALGLSRQNLQYRIRKYRIDKEWG
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNREMETLLSMYEQILEMIDLGVHAVDQQGKTVIYNRKMRDIEGMDVEDVLDKNILDVFR
CCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
FHPEQPSTLLEALRTGESILNKQQTYFNNKGQAVTTINQTHPLWKDGCIIGAVEIAKDVT
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHH
KFRKLIAEQHQRGELSRSLAGIIVQSEAMQAAIRLARHAARSEAPVLLMGEKGTGKDLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHH
SCIHYESERRLKPFLAQTCLSLPDDWMETMLFGSEENGEIQPGLFEQADGGTVLLDDVDS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCC
LSLPLQQKLVHFLQEKQFTRVHGHKPVRADVRLITSTSGDPIDAVQTGELLKSLYYQLAV
CCCCHHHHHHHHHHHHHHHHHCCCCCCEECEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
HCIVLPPLRERREDILPLADHFIRQGNERYGLNVEGLADDVQEAFLAYDWPGNVRELETV
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHH
IDETLEMMDEGETITFDHLPASFRMKLAPDAPKTDFLFNTEDILPLEKYMEEVEIYYIRK
HHHHHHHHCCCCEEEEECCCCHHEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHH
ALQHHGFNITKTAKALGLSRQNLQYRIRKYRIDKEWG
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113162; 9384377 [H]