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The map label for this gene is trmB [H]

Identifier: 13508261

GI number: 13508261

Start: 643251

End: 643883

Strand: Direct

Name: trmB [H]

Synonym: MPN522

Alternate gene names: 13508261

Gene position: 643251-643883 (Clockwise)

Preceding gene: 13508260

Following gene: 13508264

Centisome position: 78.79

GC content: 37.91

Gene sequence:

>633_bases
GTGCGCTTAAGAAAAGTTAAAGATGCTCTTGTAAGGGTACAAGACAGCCCTTACTTTAGTAACCCAGAACAACTGTCAAC
TATTGAAAGTGAATCGCTCTTCGTGGAAATAGGTTGTGGTAAAAGTTGGTTTTTAAGCCAGCAAGCACAGCAAAACCCAA
AGTGTTTTTTTGTGGGAATCGAGCGTGAACCCACGATTGTTTTAAAGGCAATTAATAAGGTAAACCGCTTAGAGGTTAAA
CCACGTAATTTATTAATAACGTGTTTGGATGCCAACCAGCTAACAGAGTACTTAAAACCACAATCGATTAGCAAAATCTT
CATTAACTTCCCCGATCCTTGACCAAAAAAGCGTCACACGTTGAGGCGACTAACTGCTCCACATTTCCTCAAGCAGTTTC
ACCAACTTTTAAAAAAGCAAGGTTTAATAGAATTCAAAACGGACAACGATCAGTTGTTTGAATTTACCTTAGAAGTCTTG
CAGAAAACAGTAGCTCACTTTAAGATAATTGAGCAAACGACAGATCTGCACAATTCACCTTTGACTAGTACCAATATTAT
GACCGAATACGAACAGCGCTTTGTTAGTTTAGGGGTTAAGATTAAAAAGCTAACGCTTGAAAAACTAAATTAG

Upstream 100 bases:

>100_bases
CGATTTGAAACCCTGTAAGAAGCATTAACTTGTCCAATACAGTAGCTTGTGTACTGTATGAGTATGCTAAACAAAATCAT
TACTTTAATTTAGATAAACA

Downstream 100 bases:

>100_bases
TAAAAATTAGAATAATTACTGCCTTAATGGCGATTGTGGCGAAGTGGTTAACGCACCTGATTGTGGATCAGGCATTCGTG
GGTTCAATTCCCATCAGTCG

Product: methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGIEREPTIVLKAINKVNRLEVK
PRNLLITCLDANQLTEYLKPQSISKIFINFPDPWPKKRHTLRRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVL
QKTVAHFKIIEQTTDLHNSPLTSTNIMTEYEQRFVSLGVKIKKLTLEKLN

Sequences:

>Translated_210_residues
MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGIEREPTIVLKAINKVNRLEVK
PRNLLITCLDANQLTEYLKPQSISKIFINFPDP*PKKRHTLRRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVL
QKTVAHFKIIEQTTDLHNSPLTSTNIMTEYEQRFVSLGVKIKKLTLEKLN
>Mature_210_residues
MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGIEREPTIVLKAINKVNRLEVK
PRNLLITCLDANQLTEYLKPQSISKIFINFPDP*PKKRHTLRRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVL
QKTVAHFKIIEQTTDLHNSPLTSTNIMTEYEQRFVSLGVKIKKLTLEKLN

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 24338; Mature: 24338

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGI
CCHHHHHHHHHEECCCCCCCCHHHHHHCCCCCEEEEECCCCHHHHCHHHCCCCCEEEEEE
EREPTIVLKAINKVNRLEVKPRNLLITCLDANQLTEYLKPQSISKIFINFPDPPKKRHTL
CCCCCHHHHHHHHHHHEEECCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
RRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVLQKTVAHFKIIEQTTDLHNSPL
HHHCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TSTNIMTEYEQRFVSLGVKIKKLTLEKLN
CCHHHHHHHHHHHHHHCCEEEEEEHCCCC
>Mature Secondary Structure
MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGI
CCHHHHHHHHHEECCCCCCCCHHHHHHCCCCCEEEEECCCCHHHHCHHHCCCCCEEEEEE
EREPTIVLKAINKVNRLEVKPRNLLITCLDANQLTEYLKPQSISKIFINFPDPPKKRHTL
CCCCCHHHHHHHHHHHEEECCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
RRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVLQKTVAHFKIIEQTTDLHNSPL
HHHCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TSTNIMTEYEQRFVSLGVKIKKLTLEKLN
CCHHHHHHHHHHHHHHCCEEEEEEHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]