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The map label for this gene is bphF [H]

Identifier: 13474525

GI number: 13474525

Start: 4341728

End: 4342522

Strand: Reverse

Name: bphF [H]

Synonym: mll5426

Alternate gene names: 13474525

Gene position: 4342522-4341728 (Counterclockwise)

Preceding gene: 13474527

Following gene: 13474524

Centisome position: 61.72

GC content: 66.54

Gene sequence:

>795_bases
ATGAGTTTGGACCAGACGACAAAGGGGATCGGCATGAGCGAATTCCGCCAGAAATGCATCGGCAAGGCAAACCTCGTCGG
TTCGTTTGCCGCCATTCCCCATCCCGTCGCGGTCGAAGTGATGGCGCTGTCGGGCCTCGACTTCCTCTGCGTGGACTGGG
AGCATGCGCAGATCTCGCGCGACATGATCGAGAGCATGGTGCGCGCGGCCGACGTCCACCGCGTGCCAGCGATGGTGCGC
GTTCCCGGCCATGCGCCGGAAGCCATCCAGGCGGCGCTGGACAGCGGCGCGCAAGGGGTGCTGGTGCCGCGCGTCTCGAC
CGCCGCGCAGGCGGCGATGGTCGTCAAAGCCTCACGCTATCCGCCGCTGGGCGAGCGTGGCGTCGGGCCTGGCCGGGCCG
CCGGCTATGGCTATCGCATTCCCGAATATCTTGCCAGCGCCAACGACAGAACCGTCGTCGGGGTGCAGGTCGAGACAGCG
GAGGGGCTCGCCAACATCGAAGCGATCGCGGCCGTCGGCGGCGTCGACGTGATCTTCGTCGGCCCCGGCGACCTTTCCGT
GTCGATCGATGCGATCGGATCGCAGGGCGCCGACAAGCTCAATGCGGCGATCCGGACGATCATTGCTGCCACGAGCGCAC
ATGGAAAGACATCCGGCATATTTTGCGCCAGCCCACAGGCTGTCGGCCGATGGGCAGCCTTCGGCGCGAGTTTCTTCGTG
CTGGCCAGCGACACGATGTTTCTGGGCGCCGGCGCCGCGGCCAACGCTGCTGCCGCACGCGACGAATTGGCATAG

Upstream 100 bases:

>100_bases
CTTTGATCATCTCGGGAACTCGCGGCGGGAGGCCACACGCTAGGCCCGCGGCAAAATATTTCAAGCCTGAAATAGCTTCG
CCTCGCCGCCGTTTGCGGAT

Downstream 100 bases:

>100_bases
ACCGGCGCGGCCAGACAAAAAGGCGTATCGCCGGAGCAACACGCGCGCCAAGCTTTTTAAGCTTAAAACTTTCGCCTTGA
AAAGCGTCCCACTTTGGATA

Product: 4-hydroxy-2-oxovalerate aldolase

Products: NA

Alternate protein names: HOA [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSLDQTTKGIGMSEFRQKCIGKANLVGSFAAIPHPVAVEVMALSGLDFLCVDWEHAQISRDMIESMVRAADVHRVPAMVR
VPGHAPEAIQAALDSGAQGVLVPRVSTAAQAAMVVKASRYPPLGERGVGPGRAAGYGYRIPEYLASANDRTVVGVQVETA
EGLANIEAIAAVGGVDVIFVGPGDLSVSIDAIGSQGADKLNAAIRTIIAATSAHGKTSGIFCASPQAVGRWAAFGASFFV
LASDTMFLGAGAAANAAAARDELA

Sequences:

>Translated_264_residues
MSLDQTTKGIGMSEFRQKCIGKANLVGSFAAIPHPVAVEVMALSGLDFLCVDWEHAQISRDMIESMVRAADVHRVPAMVR
VPGHAPEAIQAALDSGAQGVLVPRVSTAAQAAMVVKASRYPPLGERGVGPGRAAGYGYRIPEYLASANDRTVVGVQVETA
EGLANIEAIAAVGGVDVIFVGPGDLSVSIDAIGSQGADKLNAAIRTIIAATSAHGKTSGIFCASPQAVGRWAAFGASFFV
LASDTMFLGAGAAANAAAARDELA
>Mature_263_residues
SLDQTTKGIGMSEFRQKCIGKANLVGSFAAIPHPVAVEVMALSGLDFLCVDWEHAQISRDMIESMVRAADVHRVPAMVRV
PGHAPEAIQAALDSGAQGVLVPRVSTAAQAAMVVKASRYPPLGERGVGPGRAAGYGYRIPEYLASANDRTVVGVQVETAE
GLANIEAIAAVGGVDVIFVGPGDLSVSIDAIGSQGADKLNAAIRTIIAATSAHGKTSGIFCASPQAVGRWAAFGASFFVL
ASDTMFLGAGAAANAAAARDELA

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-oxovalerate to pyruvate and acetaldehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=232, Percent_Identity=34.4827586206897, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1788578, Length=212, Percent_Identity=40.0943396226415, Blast_Score=135, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 26987; Mature: 26856

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDQTTKGIGMSEFRQKCIGKANLVGSFAAIPHPVAVEVMALSGLDFLCVDWEHAQISR
CCCCCCCCCCCHHHHHHHHCCCHHHCCHHHCCCCCHHEEEEEECCCEEEEECCHHHHHHH
DMIESMVRAADVHRVPAMVRVPGHAPEAIQAALDSGAQGVLVPRVSTAAQAAMVVKASRY
HHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHEEECCCC
PPLGERGVGPGRAAGYGYRIPEYLASANDRTVVGVQVETAEGLANIEAIAAVGGVDVIFV
CCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCCCHHHHHHHHEECCEEEEEE
GPGDLSVSIDAIGSQGADKLNAAIRTIIAATSAHGKTSGIFCASPQAVGRWAAFGASFFV
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHCCEEEE
LASDTMFLGAGAAANAAAARDELA
EECCEEEEECCCCCCHHHHHHCCC
>Mature Secondary Structure 
SLDQTTKGIGMSEFRQKCIGKANLVGSFAAIPHPVAVEVMALSGLDFLCVDWEHAQISR
CCCCCCCCCCHHHHHHHHCCCHHHCCHHHCCCCCHHEEEEEECCCEEEEECCHHHHHHH
DMIESMVRAADVHRVPAMVRVPGHAPEAIQAALDSGAQGVLVPRVSTAAQAAMVVKASRY
HHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHEEECCCC
PPLGERGVGPGRAAGYGYRIPEYLASANDRTVVGVQVETAEGLANIEAIAAVGGVDVIFV
CCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCCCHHHHHHHHEECCEEEEEE
GPGDLSVSIDAIGSQGADKLNAAIRTIIAATSAHGKTSGIFCASPQAVGRWAAFGASFFV
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHCCEEEE
LASDTMFLGAGAAANAAAARDELA
EECCEEEEECCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10049392 [H]