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The map label for this gene is prpB [C]

Identifier: 13474454

GI number: 13474454

Start: 4266590

End: 4267393

Strand: Reverse

Name: prpB [C]

Synonym: mll5340

Alternate gene names: 13474454

Gene position: 4267393-4266590 (Counterclockwise)

Preceding gene: 13474455

Following gene: 13474451

Centisome position: 60.65

GC content: 64.8

Gene sequence:

>804_bases
ATGGACAAGGGCAAGATCTTTCGCGACCTGCATGCCTCGACCTTCGTCATGCCCAATCCCTGGGATCCGGGCACGACCAA
GCTGCTCGGATCCTTCGGCTTCAAGGCGCTGGCGACGACCAGCGCCGGCTTTGCCTTTTCGCGCGGCCTGCCGGACGGCG
CGGTGACCTTCGAGCAGATGATCCATCACTGCCGCGAGGTGACAGCGGCGACCGGCTTGCCGGTCTCGGCCGACTTAGAG
AAGGGCAAGGGCGACAGCGCCGAGCGGGCCGCCGAAACCATCTTCGCGGCAGAAGCCGCAGGCCTTGCCGGCTGCTCGAT
CGAGGACCACACCGGCGATCCCGACAGGCCGATCTATGATTTCTCACACGCGGTCGAGCGCGTGGCGGCGGCGGTGGAGG
CGGCGCGGGCCTTGAGACGCGATTTCGTCTTCACGGCGCGGGCCGAGAATTTCCTATGGGGCAAGTCCGATCTCGACGAC
ACCATCAAGCGGCTGCAGGCCTTCGAGAAGGCCGGCGCCGACGTGCTCTATGCGCCTGGGATCGGCGACGTCGAGATGGT
GCGCACGCTGTGTTCGGCGGTCAGCAAGCCGGTCAACGTCATGGCAAAACCGGGCTTCACCATCGCCGATCTCGCAAAGG
CCGGAGTGAAGCGCATTTCGCTCGGACCGTGGCTGACCAATTTCGCCTATGGCATGCTGGAGACGGCGACGCGCGAAATC
CAGCAGGACGGCACGTTCGGCTTCACCCGGGCCGCCATGCCATTCGGCAAATTGCAGGCGCTGTTCGGCAAATCCCAGGA
TTAA

Upstream 100 bases:

>100_bases
CAGCAGAGAACGAGAAACGCTTCTGAACCAAGCGCATTCATTTGCCCGGCCGAAGGCGGCGCGATAGCATCCGTCCCGAC
ACTCGTGATGGGGAGCATTC

Downstream 100 bases:

>100_bases
ACGGCGGCCGTCGCATGACGACTGCGGTCACCGGCTACTTTCGCGGGCCGAAATGGCCTTGTGCAGATGCACCATCATGG
CGGCGGCGAAGAGCGGCGTC

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MDKGKIFRDLHASTFVMPNPWDPGTTKLLGSFGFKALATTSAGFAFSRGLPDGAVTFEQMIHHCREVTAATGLPVSADLE
KGKGDSAERAAETIFAAEAAGLAGCSIEDHTGDPDRPIYDFSHAVERVAAAVEAARALRRDFVFTARAENFLWGKSDLDD
TIKRLQAFEKAGADVLYAPGIGDVEMVRTLCSAVSKPVNVMAKPGFTIADLAKAGVKRISLGPWLTNFAYGMLETATREI
QQDGTFGFTRAAMPFGKLQALFGKSQD

Sequences:

>Translated_267_residues
MDKGKIFRDLHASTFVMPNPWDPGTTKLLGSFGFKALATTSAGFAFSRGLPDGAVTFEQMIHHCREVTAATGLPVSADLE
KGKGDSAERAAETIFAAEAAGLAGCSIEDHTGDPDRPIYDFSHAVERVAAAVEAARALRRDFVFTARAENFLWGKSDLDD
TIKRLQAFEKAGADVLYAPGIGDVEMVRTLCSAVSKPVNVMAKPGFTIADLAKAGVKRISLGPWLTNFAYGMLETATREI
QQDGTFGFTRAAMPFGKLQALFGKSQD
>Mature_267_residues
MDKGKIFRDLHASTFVMPNPWDPGTTKLLGSFGFKALATTSAGFAFSRGLPDGAVTFEQMIHHCREVTAATGLPVSADLE
KGKGDSAERAAETIFAAEAAGLAGCSIEDHTGDPDRPIYDFSHAVERVAAAVEAARALRRDFVFTARAENFLWGKSDLDD
TIKRLQAFEKAGADVLYAPGIGDVEMVRTLCSAVSKPVNVMAKPGFTIADLAKAGVKRISLGPWLTNFAYGMLETATREI
QQDGTFGFTRAAMPFGKLQALFGKSQD

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=197, Percent_Identity=34.010152284264, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 28631; Mature: 28631

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKGKIFRDLHASTFVMPNPWDPGTTKLLGSFGFKALATTSAGFAFSRGLPDGAVTFEQM
CCCCHHHHHHCCCEEECCCCCCCHHHHHHHHHCHHHHHHCCCCCHHHCCCCCHHHHHHHH
IHHCREVTAATGLPVSADLEKGKGDSAERAAETIFAAEAAGLAGCSIEDHTGDPDRPIYD
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
FSHAVERVAAAVEAARALRRDFVFTARAENFLWGKSDLDDTIKRLQAFEKAGADVLYAPG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCC
IGDVEMVRTLCSAVSKPVNVMAKPGFTIADLAKAGVKRISLGPWLTNFAYGMLETATREI
CCCHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QQDGTFGFTRAAMPFGKLQALFGKSQD
HHCCCCCHHHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure
MDKGKIFRDLHASTFVMPNPWDPGTTKLLGSFGFKALATTSAGFAFSRGLPDGAVTFEQM
CCCCHHHHHHCCCEEECCCCCCCHHHHHHHHHCHHHHHHCCCCCHHHCCCCCHHHHHHHH
IHHCREVTAATGLPVSADLEKGKGDSAERAAETIFAAEAAGLAGCSIEDHTGDPDRPIYD
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
FSHAVERVAAAVEAARALRRDFVFTARAENFLWGKSDLDDTIKRLQAFEKAGADVLYAPG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCC
IGDVEMVRTLCSAVSKPVNVMAKPGFTIADLAKAGVKRISLGPWLTNFAYGMLETATREI
CCCHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QQDGTFGFTRAAMPFGKLQALFGKSQD
HHCCCCCHHHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]