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The map label for this gene is trpS

Identifier: 13474436

GI number: 13474436

Start: 4243131

End: 4244198

Strand: Reverse

Name: trpS

Synonym: mll5317

Alternate gene names: 13474436

Gene position: 4244198-4243131 (Counterclockwise)

Preceding gene: 13474437

Following gene: 13474435

Centisome position: 60.32

GC content: 62.73

Gene sequence:

>1068_bases
ATGTCCGCCTTCAAGCCACTCGTCTTCTCCGGCGTCCAGCCGACCGGCAATCTGCATCTCGGCAACTATCTTGGCGCCAT
CAAGAAATTCGTCGCCCTGCAGGAAACGTCCGATTGCATCTACTGCGTCGTCGACCTGCATTCGCTGACCGCGCAGTTGG
TCCATGAGGACCTCGCCGACCAGACGCGCTCGATCACCGCCGCCTTCCTTGCCTCGGGCATCGACCCGAAGAAGCACATC
GTCTTCAACCAGTCGCGGGTGATGCAGCATGCCGAGCTTGCCTGGATCTTCAATTGCGTCGCGCGCATCGGCTGGATGAA
CAAGATGACGCAGTTCAAGGACAAGGCCGGCAAGGACCGCGAAAATGCCTCGCTGGGCCTGCTCGCCTATCCCTCTTTGA
TGGCTGCCGACATCCTGCTCTACCGCGCCACCCATGTGCCGGTGGGCGAGGACCAGAAACAGCATCTGGAGCTGACCCGC
GACATCGCCCAGAAATTCAACAATGATTTTTCCGATCGCATCGCCCGGCTTGGCGTCGGCGTCGAGATGCAGGTCGGCGA
GGAGACGGTGAACGGCTTCTTCCCGATCACCGAACCGGTGATCGGCGGCCCGGCGGCGCGCATCATGAGCCTGCGCGACG
GTTCGAAGAAGATGTCGAAGTCGGATCCGTCGGACCTGTCGCGCATCAACCTGACCGACGATGCCGACACCATCTCGAAA
AAGATCCGCAAGGCCAAGACCGATCCCGAGGCCTTGCCGAGCGAAGTCAGCGGGCTGGAAAGCCGGCCGGAAGCGGAAAA
CCTGGTCGGCATCTATGCCGGGCTGGCCGAGATGTCGAAGGCCGACGTGCTGAAGGAATTCGGCGGCCAGCAGTTTTCAG
TCTTCAAGCCGGCGCTGGCCGACCTTGCGGTGGAGAAGCTGGCGCCGATCGCCGGCGAGATGCGCCGCATCGAAGGCGAC
CGCGCCTATGTCGACGCGGTGCTCAGGGACGGCGGCGAACGCGCCGGCGTGCTCGCCGAAACGACGATGAAGACGGTGCG
CGATATTATCGGCCTGCTGCAGGGCTGA

Upstream 100 bases:

>100_bases
TTTCGCCGCGTGCGGTTTCCAGCCGCATGATCGGCTCCAAAAAGTCTGCAACTTTTTGGAATCATGCTTATACGGCCCTC
CACAAGCCATGAGGAAACCC

Downstream 100 bases:

>100_bases
TCCGCATCGTCAGTGCACCGCACCATTCCAGCACAAGACATACTGGCCGAAGGTCGGCGGCTTGCCGCCGGTCGGCGGCG
GTGGCAGATTGCGGCCGAAT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSAFKPLVFSGVQPTGNLHLGNYLGAIKKFVALQETSDCIYCVVDLHSLTAQLVHEDLADQTRSITAAFLASGIDPKKHI
VFNQSRVMQHAELAWIFNCVARIGWMNKMTQFKDKAGKDRENASLGLLAYPSLMAADILLYRATHVPVGEDQKQHLELTR
DIAQKFNNDFSDRIARLGVGVEMQVGEETVNGFFPITEPVIGGPAARIMSLRDGSKKMSKSDPSDLSRINLTDDADTISK
KIRKAKTDPEALPSEVSGLESRPEAENLVGIYAGLAEMSKADVLKEFGGQQFSVFKPALADLAVEKLAPIAGEMRRIEGD
RAYVDAVLRDGGERAGVLAETTMKTVRDIIGLLQG

Sequences:

>Translated_355_residues
MSAFKPLVFSGVQPTGNLHLGNYLGAIKKFVALQETSDCIYCVVDLHSLTAQLVHEDLADQTRSITAAFLASGIDPKKHI
VFNQSRVMQHAELAWIFNCVARIGWMNKMTQFKDKAGKDRENASLGLLAYPSLMAADILLYRATHVPVGEDQKQHLELTR
DIAQKFNNDFSDRIARLGVGVEMQVGEETVNGFFPITEPVIGGPAARIMSLRDGSKKMSKSDPSDLSRINLTDDADTISK
KIRKAKTDPEALPSEVSGLESRPEAENLVGIYAGLAEMSKADVLKEFGGQQFSVFKPALADLAVEKLAPIAGEMRRIEGD
RAYVDAVLRDGGERAGVLAETTMKTVRDIIGLLQG
>Mature_354_residues
SAFKPLVFSGVQPTGNLHLGNYLGAIKKFVALQETSDCIYCVVDLHSLTAQLVHEDLADQTRSITAAFLASGIDPKKHIV
FNQSRVMQHAELAWIFNCVARIGWMNKMTQFKDKAGKDRENASLGLLAYPSLMAADILLYRATHVPVGEDQKQHLELTRD
IAQKFNNDFSDRIARLGVGVEMQVGEETVNGFFPITEPVIGGPAARIMSLRDGSKKMSKSDPSDLSRINLTDDADTISKK
IRKAKTDPEALPSEVSGLESRPEAENLVGIYAGLAEMSKADVLKEFGGQQFSVFKPALADLAVEKLAPIAGEMRRIEGDR
AYVDAVLRDGGERAGVLAETTMKTVRDIIGLLQG

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=354, Percent_Identity=41.2429378531073, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI41352700, Length=202, Percent_Identity=46.039603960396, Blast_Score=181, Evalue=9e-46,
Organism=Escherichia coli, GI1789786, Length=349, Percent_Identity=39.8280802292264, Blast_Score=270, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI71982800, Length=358, Percent_Identity=33.2402234636872, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71982793, Length=358, Percent_Identity=33.2402234636872, Blast_Score=187, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6320474, Length=365, Percent_Identity=35.6164383561644, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24666151, Length=348, Percent_Identity=37.6436781609195, Blast_Score=217, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_RHILO (Q98C31)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106004.1
- ProteinModelPortal:   Q98C31
- SMR:   Q98C31
- GeneID:   1228665
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll5317
- NMPDR:   fig|266835.1.peg.4108
- HOGENOM:   HBG293263
- OMA:   NKPGVSN
- ProtClustDB:   PRK00927
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 38761; Mature: 38630

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFKPLVFSGVQPTGNLHLGNYLGAIKKFVALQETSDCIYCVVDLHSLTAQLVHEDLAD
CCCCCCHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHH
QTRSITAAFLASGIDPKKHIVFNQSRVMQHAELAWIFNCVARIGWMNKMTQFKDKAGKDR
HHHHHHHHHHHCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ENASLGLLAYPSLMAADILLYRATHVPVGEDQKQHLELTRDIAQKFNNDFSDRIARLGVG
CCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
VEMQVGEETVNGFFPITEPVIGGPAARIMSLRDGSKKMSKSDPSDLSRINLTDDADTISK
CEEECCHHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHCCCCCCHHHEECCCCCHHHHHH
KIRKAKTDPEALPSEVSGLESRPEAENLVGIYAGLAEMSKADVLKEFGGQQFSVFKPALA
HHHHCCCCHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHH
DLAVEKLAPIAGEMRRIEGDRAYVDAVLRDGGERAGVLAETTMKTVRDIIGLLQG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAFKPLVFSGVQPTGNLHLGNYLGAIKKFVALQETSDCIYCVVDLHSLTAQLVHEDLAD
CCCCCHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHH
QTRSITAAFLASGIDPKKHIVFNQSRVMQHAELAWIFNCVARIGWMNKMTQFKDKAGKDR
HHHHHHHHHHHCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ENASLGLLAYPSLMAADILLYRATHVPVGEDQKQHLELTRDIAQKFNNDFSDRIARLGVG
CCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
VEMQVGEETVNGFFPITEPVIGGPAARIMSLRDGSKKMSKSDPSDLSRINLTDDADTISK
CEEECCHHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHCCCCCCHHHEECCCCCHHHHHH
KIRKAKTDPEALPSEVSGLESRPEAENLVGIYAGLAEMSKADVLKEFGGQQFSVFKPALA
HHHHCCCCHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHH
DLAVEKLAPIAGEMRRIEGDRAYVDAVLRDGGERAGVLAETTMKTVRDIIGLLQG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968