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The map label for this gene is aroA

Identifier: 13474346

GI number: 13474346

Start: 4144912

End: 4146270

Strand: Reverse

Name: aroA

Synonym: mll5213

Alternate gene names: 13474346

Gene position: 4146270-4144912 (Counterclockwise)

Preceding gene: 13474359

Following gene: 13474345

Centisome position: 58.93

GC content: 67.4

Gene sequence:

>1359_bases
ATGTCTCACGCCGCCGCTGCCAAGCCGGCCACCGCCCGCAAATCTCAAGCCCTTTCCGGCACCGCGCGCGTGCCGGGCGA
CAAGTCGATCTCGCATCGCTCCTTCATGTTCGGCGGCCTCGCCTCCGGCGAAACCCGCATCACCGGCCTGCTCGAGGGCG
AGGACGTGATGCGCACCGGCGCGGCCATGAAGGCGATGGGCGCGCATATCGAAAAGCGCGGCGCCGAATGGGTGATCCGC
GGCACCGGCAACGGCGCGCTCTTACAGCCGGAAGGCCCGCTCGACTTCGGCAATGCCGGCACCGGCTCACGGCTGACCAT
GGGCCTCGTCGGCACCTACGACATGGAAACCACCTTCATCGGCGATGCCTCGCTGTCCGGCCGGCCGATGGGCCGCGTGC
TGGAGCCGCTGCGCCAGATGGGCGTGCAGGTGTTGAAGGCGACGCCCGGCGACCGCATGCCGATCACGCTGCATGGTCCC
AAGCACGCCGCCCCGATCACCTACCGCGTGCCGATGGCTTCGGCACAGGTCAAATCGGCGGTGCTGCTCGCCGGCCTCAA
TACGCCCGGCATCACCACCGTCATCGAACCGGTGATGACGCGCGACCACACCGAAAAGATGCTGAAAGGCTTCGGCGCCA
ATCTGTCGGTCGAGACCGACGAGCGCGGCGTGCGCCATATCTTCATCGAAGGCCAGGGCAGACTGACCGGCCAGACGATC
GCCGTGCCCGGCGATCCGTCCTCGGCCGGCTTCCCGCTGGTGGCGGCGCTGATCGTGCCGGGTTCGGACATCACGATCGA
AAACGTGCTGATGAACCCGACCCGCACCGGCTTGCTGCTGACCCTGCAGGAAATGGGCGGCCAGATCGACATTTTGAACC
CGCGCAATGCCGGCGGCGAGGACGTCGCCGATCTGCGCGTGCGCTATTCCGAACTCAAGGGCGTCGCGGTGCCGCCGGAG
CGGGCACCGTCGATGATCGACGAGTACCCGGTGCTCGCTGTCGCTGCCAGCTTCGCCGAGGGCGAGACGCTGATGCAAGG
GCTGGAAGAATTGCGCGTCAAGGAATCCGACCGGCTGTCGGCCGTCGCCAACGGGCTGAAGCTCAACGGCGTCGACTGCA
CCGAGGGCGAGGCGTCGCTTGCCGTGCGCGGCAGGCCGGGCGGCAAGGGGCTCGGCGGCCATCCCAACGGCCTGGATACG
ACCGTGCAGACCCATCTCGACCACCGCATCGCCATGAGCTTTCTGGTGATGGGACTGGCGACGGAAAAGCCGGTCACCAT
CGACGATCAGGCGATGATCGCGACGAGCTTTCCGGAGTTCATGGGGCTGATGACGGGGCTCGGGGCGGAGATCAGGTGA

Upstream 100 bases:

>100_bases
GTGACGACCATGGGGATGACCATTTCGCCCCGCCGTGATACGAGACCGCCGCAACAAATGAAAAGCGCCGGCCCGCCGGC
ATTCCGTCCAGGGAAATTCC

Downstream 100 bases:

>100_bases
CGGTCGTGGCAACCAACAGCCGTGGCACGTTGGCAGGCGGCGAAGGATCTCGGCGCCCAACCCTTTCGTTGGCCAGCCAA
TGACTCACGTCTTCACCATC

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSHAAAAKPATARKSQALSGTARVPGDKSISHRSFMFGGLASGETRITGLLEGEDVMRTGAAMKAMGAHIEKRGAEWVIR
GTGNGALLQPEGPLDFGNAGTGSRLTMGLVGTYDMETTFIGDASLSGRPMGRVLEPLRQMGVQVLKATPGDRMPITLHGP
KHAAPITYRVPMASAQVKSAVLLAGLNTPGITTVIEPVMTRDHTEKMLKGFGANLSVETDERGVRHIFIEGQGRLTGQTI
AVPGDPSSAGFPLVAALIVPGSDITIENVLMNPTRTGLLLTLQEMGGQIDILNPRNAGGEDVADLRVRYSELKGVAVPPE
RAPSMIDEYPVLAVAASFAEGETLMQGLEELRVKESDRLSAVANGLKLNGVDCTEGEASLAVRGRPGGKGLGGHPNGLDT
TVQTHLDHRIAMSFLVMGLATEKPVTIDDQAMIATSFPEFMGLMTGLGAEIR

Sequences:

>Translated_452_residues
MSHAAAAKPATARKSQALSGTARVPGDKSISHRSFMFGGLASGETRITGLLEGEDVMRTGAAMKAMGAHIEKRGAEWVIR
GTGNGALLQPEGPLDFGNAGTGSRLTMGLVGTYDMETTFIGDASLSGRPMGRVLEPLRQMGVQVLKATPGDRMPITLHGP
KHAAPITYRVPMASAQVKSAVLLAGLNTPGITTVIEPVMTRDHTEKMLKGFGANLSVETDERGVRHIFIEGQGRLTGQTI
AVPGDPSSAGFPLVAALIVPGSDITIENVLMNPTRTGLLLTLQEMGGQIDILNPRNAGGEDVADLRVRYSELKGVAVPPE
RAPSMIDEYPVLAVAASFAEGETLMQGLEELRVKESDRLSAVANGLKLNGVDCTEGEASLAVRGRPGGKGLGGHPNGLDT
TVQTHLDHRIAMSFLVMGLATEKPVTIDDQAMIATSFPEFMGLMTGLGAEIR
>Mature_451_residues
SHAAAAKPATARKSQALSGTARVPGDKSISHRSFMFGGLASGETRITGLLEGEDVMRTGAAMKAMGAHIEKRGAEWVIRG
TGNGALLQPEGPLDFGNAGTGSRLTMGLVGTYDMETTFIGDASLSGRPMGRVLEPLRQMGVQVLKATPGDRMPITLHGPK
HAAPITYRVPMASAQVKSAVLLAGLNTPGITTVIEPVMTRDHTEKMLKGFGANLSVETDERGVRHIFIEGQGRLTGQTIA
VPGDPSSAGFPLVAALIVPGSDITIENVLMNPTRTGLLLTLQEMGGQIDILNPRNAGGEDVADLRVRYSELKGVAVPPER
APSMIDEYPVLAVAASFAEGETLMQGLEELRVKESDRLSAVANGLKLNGVDCTEGEASLAVRGRPGGKGLGGHPNGLDTT
VQTHLDHRIAMSFLVMGLATEKPVTIDDQAMIATSFPEFMGLMTGLGAEIR

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=439, Percent_Identity=29.1571753986333, Blast_Score=118, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6320332, Length=468, Percent_Identity=26.0683760683761, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_RHILO (Q98CC1)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105914.1
- ProteinModelPortal:   Q98CC1
- SMR:   Q98CC1
- GeneID:   1228575
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll5213
- NMPDR:   fig|266835.1.peg.4018
- HOGENOM:   HBG646626
- OMA:   LSRNHTE
- ProtClustDB:   PRK02427
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 47456; Mature: 47325

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHAAAAKPATARKSQALSGTARVPGDKSISHRSFMFGGLASGETRITGLLEGEDVMRTG
CCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCHHHHHHH
AAMKAMGAHIEKRGAEWVIRGTGNGALLQPEGPLDFGNAGTGSRLTMGLVGTYDMETTFI
HHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCCCCCCCCCEEEEEEEEEECCCEEEE
GDASLSGRPMGRVLEPLRQMGVQVLKATPGDRMPITLHGPKHAAPITYRVPMASAQVKSA
ECCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEECCCCCCCCEEEECCCCHHHHCCE
VLLAGLNTPGITTVIEPVMTRDHTEKMLKGFGANLSVETDERGVRHIFIEGQGRLTGQTI
EEEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCCCEE
AVPGDPSSAGFPLVAALIVPGSDITIENVLMNPTRTGLLLTLQEMGGQIDILNPRNAGGE
EECCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEHHHHCCEEEEECCCCCCCC
DVADLRVRYSELKGVAVPPERAPSMIDEYPVLAVAASFAEGETLMQGLEELRVKESDRLS
CHHHHHHHHHHCCCCCCCCHHCCHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHH
AVANGLKLNGVDCTEGEASLAVRGRPGGKGLGGHPNGLDTTVQTHLDHRIAMSFLVMGLA
HHHCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
TEKPVTIDDQAMIATSFPEFMGLMTGLGAEIR
CCCCEEECCCEEEECCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SHAAAAKPATARKSQALSGTARVPGDKSISHRSFMFGGLASGETRITGLLEGEDVMRTG
CCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCHHHHHHH
AAMKAMGAHIEKRGAEWVIRGTGNGALLQPEGPLDFGNAGTGSRLTMGLVGTYDMETTFI
HHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCCCCCCCCCEEEEEEEEEECCCEEEE
GDASLSGRPMGRVLEPLRQMGVQVLKATPGDRMPITLHGPKHAAPITYRVPMASAQVKSA
ECCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEECCCCCCCCEEEECCCCHHHHCCE
VLLAGLNTPGITTVIEPVMTRDHTEKMLKGFGANLSVETDERGVRHIFIEGQGRLTGQTI
EEEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCCCEE
AVPGDPSSAGFPLVAALIVPGSDITIENVLMNPTRTGLLLTLQEMGGQIDILNPRNAGGE
EECCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEHHHHCCEEEEECCCCCCCC
DVADLRVRYSELKGVAVPPERAPSMIDEYPVLAVAASFAEGETLMQGLEELRVKESDRLS
CHHHHHHHHHHCCCCCCCCHHCCHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHH
AVANGLKLNGVDCTEGEASLAVRGRPGGKGLGGHPNGLDTTVQTHLDHRIAMSFLVMGLA
HHHCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
TEKPVTIDDQAMIATSFPEFMGLMTGLGAEIR
CCCCEEECCCEEEECCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968