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The map label for this gene is argT [H]

Identifier: 13474337

GI number: 13474337

Start: 4134373

End: 4135170

Strand: Reverse

Name: argT [H]

Synonym: mll5202

Alternate gene names: 13474337

Gene position: 4135170-4134373 (Counterclockwise)

Preceding gene: 13474338

Following gene: 13474336

Centisome position: 58.77

GC content: 61.65

Gene sequence:

>798_bases
ATGAAGACTGTTCTGAAGACATTCGCCGCGGCACTGCTGCTCGGCGTCGCCGCCATGGGCGTGGCCAAAGCCGATCCGGT
CAAGATCGGCGTCGCCGCCGAGCCCTATCCGCCCTTCACCTCGCCGGATGCCTCGGGCAAATGGGTCGGCTGGGAAATCG
ACTTCATCGACGCCGTGTGCGCCGAAGAGAAACTCGACTGCGTCATCACACCTGTTGCCTGGGACGGCATCATCCCGGCG
CTGACCACCAAGAAGATCGACCTCATCGTCTCGTCGATGTCGATCACCGACGAGCGCAAGAAGACGATCGACTTCTCGGA
CAAGTACTACAACACGCCGACGGCGATCATCGGACCGAAGGACCAGAAGTTCGGCGCCACGCCTGACGACCTCAAGGGCA
AGGTGGTCGGCGTCCAAGTGTCGACCGTGCACGCCGTCTACGCCAAGAAGCACTTCGGCTCCACGGCTTCCGAAATCAAG
GAGTACCAGACCCAGGACGAAGCCAACCAGGATCTCGCCGCCGGCCGCCTCGATGCGGTGCAGGCCGATTCCATCGCGCT
TGGCGAATTCCTGAAGTCCGACCAGGGCAAGGCCTGCTGCGACCTGAAGGGCATGGTGGCTCCGGACGACGAGGTGCTCG
GACCGGGCGTCGGCGCCGGCGTGCGCAAGGAAGACACCGACCTGAAGGAAAAGATCAACGCCGGCATCAAGGCGATCCGC
TCCAACGGCAAGTATGACGAGATCTCGAAGAAATATTTCGATTTCGACATTTACGGCGGCTCGACGCAGTCGAACTGA

Upstream 100 bases:

>100_bases
GCACCGAAATCCGAAAGGCTCAAGCAGTTCATCCGCAACATCGGCTAGGAAAGCCGGGACGGAAGAAAACCGGGAACCGA
CAACAAACTGGGAGTTTGAA

Downstream 100 bases:

>100_bases
CGCCCCTCGCATGAGCCGGAGGGCATGACGCCAAAGTTGTGCTCTCCGGCCTATTCTTCCCACGCATGCTTGCCGCCGGC
CGCTGGCGTCGGCGGACAAA

Product: amino acid ABC transporter, binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKTVLKTFAAALLLGVAAMGVAKADPVKIGVAAEPYPPFTSPDASGKWVGWEIDFIDAVCAEEKLDCVITPVAWDGIIPA
LTTKKIDLIVSSMSITDERKKTIDFSDKYYNTPTAIIGPKDQKFGATPDDLKGKVVGVQVSTVHAVYAKKHFGSTASEIK
EYQTQDEANQDLAAGRLDAVQADSIALGEFLKSDQGKACCDLKGMVAPDDEVLGPGVGAGVRKEDTDLKEKINAGIKAIR
SNGKYDEISKKYFDFDIYGGSTQSN

Sequences:

>Translated_265_residues
MKTVLKTFAAALLLGVAAMGVAKADPVKIGVAAEPYPPFTSPDASGKWVGWEIDFIDAVCAEEKLDCVITPVAWDGIIPA
LTTKKIDLIVSSMSITDERKKTIDFSDKYYNTPTAIIGPKDQKFGATPDDLKGKVVGVQVSTVHAVYAKKHFGSTASEIK
EYQTQDEANQDLAAGRLDAVQADSIALGEFLKSDQGKACCDLKGMVAPDDEVLGPGVGAGVRKEDTDLKEKINAGIKAIR
SNGKYDEISKKYFDFDIYGGSTQSN
>Mature_265_residues
MKTVLKTFAAALLLGVAAMGVAKADPVKIGVAAEPYPPFTSPDASGKWVGWEIDFIDAVCAEEKLDCVITPVAWDGIIPA
LTTKKIDLIVSSMSITDERKKTIDFSDKYYNTPTAIIGPKDQKFGATPDDLKGKVVGVQVSTVHAVYAKKHFGSTASEIK
EYQTQDEANQDLAAGRLDAVQADSIALGEFLKSDQGKACCDLKGMVAPDDEVLGPGVGAGVRKEDTDLKEKINAGIKAIR
SNGKYDEISKKYFDFDIYGGSTQSN

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=256, Percent_Identity=39.0625, Blast_Score=173, Evalue=8e-45,
Organism=Escherichia coli, GI1788648, Length=237, Percent_Identity=38.8185654008439, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=33.8645418326693, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1787088, Length=249, Percent_Identity=32.5301204819277, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787031, Length=256, Percent_Identity=34.765625, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1788228, Length=223, Percent_Identity=27.8026905829596, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28361; Mature: 28361

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVLKTFAAALLLGVAAMGVAKADPVKIGVAAEPYPPFTSPDASGKWVGWEIDFIDAVC
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHH
AEEKLDCVITPVAWDGIIPALTTKKIDLIVSSMSITDERKKTIDFSDKYYNTPTAIIGPK
HCCCCCEEEECCCCCCCCHHHHHHHEEEEEECCCCCCHHHHCCCCCCCCCCCCCEEECCC
DQKFGATPDDLKGKVVGVQVSTVHAVYAKKHFGSTASEIKEYQTQDEANQDLAAGRLDAV
CCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHCCCCHH
QADSIALGEFLKSDQGKACCDLKGMVAPDDEVLGPGVGAGVRKEDTDLKEKINAGIKAIR
CCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SNGKYDEISKKYFDFDIYGGSTQSN
CCCCHHHHHHHHEEEEEECCCCCCC
>Mature Secondary Structure
MKTVLKTFAAALLLGVAAMGVAKADPVKIGVAAEPYPPFTSPDASGKWVGWEIDFIDAVC
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHH
AEEKLDCVITPVAWDGIIPALTTKKIDLIVSSMSITDERKKTIDFSDKYYNTPTAIIGPK
HCCCCCEEEECCCCCCCCHHHHHHHEEEEEECCCCCCHHHHCCCCCCCCCCCCCEEECCC
DQKFGATPDDLKGKVVGVQVSTVHAVYAKKHFGSTASEIKEYQTQDEANQDLAAGRLDAV
CCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHCCCCHH
QADSIALGEFLKSDQGKACCDLKGMVAPDDEVLGPGVGAGVRKEDTDLKEKINAGIKAIR
CCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SNGKYDEISKKYFDFDIYGGSTQSN
CCCCHHHHHHHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]