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The map label for this gene is rph

Identifier: 13473880

GI number: 13473880

Start: 3667604

End: 3668320

Strand: Direct

Name: rph

Synonym: mlr4622

Alternate gene names: 13473880

Gene position: 3667604-3668320 (Clockwise)

Preceding gene: 13473873

Following gene: 13473881

Centisome position: 52.13

GC content: 65.69

Gene sequence:

>717_bases
ATGCGCCCCTCCAAACGCCAATTCGACGAAATGCGCGCCATCTCCTTCGAGCGCGGCGTCTCCAAGCACGCCGAAGGCTC
GTGCCTGGTGAAGTTCGGCGACACCCATGTCCTGTGCACGGCGAGCCTCGAGGAAAAGGTGCCGGGCTGGATGCGCAATT
CCGGCAAGGGCTGGGTGACGGCCGAATATGGCATGCTGCCACGCTCGACCGGCGAGCGCATGCGCCGCGAGGCCTCCGCC
GGCAAGCAGGGCGGCCGCACGCTGGAAATCCAGCGCCTGATCGGCCGTTCGCTGCGCGCCGTCGTCGATTTGCAGGCGCT
GGGCGAACAGCAGATCACCGTCGATTGCGACGTCATCCAGGCCGACGGCGGCACCCGCACCGCCTCGATCACCGGCGGCT
GGGTGGCGCTGTACGACTGCCTGCGCTGGATGGAAGCCCGGCAGATGGCCAGTGTTTCGAAGGTGCTGAAGGACCATGTC
GCGGCGATTTCCTGCGGCATCCATGACGGCCAGCCGGTCATCGACCTTGATTATCTCGAGGATTCCGCGGCCGGCACCGA
CGCCAATTTCGTCATGACCGGCAAGGGCGGCATCGTCGAGATCCAGGGCACAGCCGAGGGCGAGCCTTTCTCGGAAGACC
AGTTCGCCGAGTTGATGGGCCTGGCCAAGAAAGGCATCCAGCGCCTGGTCAGCCTGCAACAGATGGCGGTGGCGTAA

Upstream 100 bases:

>100_bases
CTCTGTATCGGATCGCACGGTCCGTTTTCCTTTGCGCGCTGCCCCCCATGCGGCTAAAAGCCGGCCACGATTCCGAAAAA
TGACTGGGAAAGAAGCCTGA

Downstream 100 bases:

>100_bases
GCCATCGATTTTCTGGTGACCCCCTCCGCCATCCTTGAATCAGCCCTGTACGTCACCGATCTGGCGGCGGCGGAACAATT
CTATGTCGGCGTGCTCGGCC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPSKRQFDEMRAISFERGVSKHAEGSCLVKFGDTHVLCTASLEEKVPGWMRNSGKGWVTAEYGMLPRSTGERMRREASA
GKQGGRTLEIQRLIGRSLRAVVDLQALGEQQITVDCDVIQADGGTRTASITGGWVALYDCLRWMEARQMASVSKVLKDHV
AAISCGIHDGQPVIDLDYLEDSAAGTDANFVMTGKGGIVEIQGTAEGEPFSEDQFAELMGLAKKGIQRLVSLQQMAVA

Sequences:

>Translated_238_residues
MRPSKRQFDEMRAISFERGVSKHAEGSCLVKFGDTHVLCTASLEEKVPGWMRNSGKGWVTAEYGMLPRSTGERMRREASA
GKQGGRTLEIQRLIGRSLRAVVDLQALGEQQITVDCDVIQADGGTRTASITGGWVALYDCLRWMEARQMASVSKVLKDHV
AAISCGIHDGQPVIDLDYLEDSAAGTDANFVMTGKGGIVEIQGTAEGEPFSEDQFAELMGLAKKGIQRLVSLQQMAVA
>Mature_238_residues
MRPSKRQFDEMRAISFERGVSKHAEGSCLVKFGDTHVLCTASLEEKVPGWMRNSGKGWVTAEYGMLPRSTGERMRREASA
GKQGGRTLEIQRLIGRSLRAVVDLQALGEQQITVDCDVIQADGGTRTASITGGWVALYDCLRWMEARQMASVSKVLKDHV
AAISCGIHDGQPVIDLDYLEDSAAGTDANFVMTGKGGIVEIQGTAEGEPFSEDQFAELMGLAKKGIQRLVSLQQMAVA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=212, Percent_Identity=61.3207547169811, Blast_Score=261, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI71981632, Length=183, Percent_Identity=28.4153005464481, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_RHILO (Q98DN6)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105448.1
- ProteinModelPortal:   Q98DN6
- SMR:   Q98DN6
- GeneID:   1228109
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr4622
- NMPDR:   fig|266835.1.peg.3552
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BRENDA:   2.7.7.56
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25852; Mature: 25852

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSKRQFDEMRAISFERGVSKHAEGSCLVKFGDTHVLCTASLEEKVPGWMRNSGKGWVT
CCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCHHHHCCCHHHCCCCCEEE
AEYGMLPRSTGERMRREASAGKQGGRTLEIQRLIGRSLRAVVDLQALGEQQITVDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTASITGGWVALYDCLRWMEARQMASVSKVLKDHVAAISCGIHDGQPVIDLDYLE
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
DSAAGTDANFVMTGKGGIVEIQGTAEGEPFSEDQFAELMGLAKKGIQRLVSLQQMAVA
CCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSKRQFDEMRAISFERGVSKHAEGSCLVKFGDTHVLCTASLEEKVPGWMRNSGKGWVT
CCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCHHHHCCCHHHCCCCCEEE
AEYGMLPRSTGERMRREASAGKQGGRTLEIQRLIGRSLRAVVDLQALGEQQITVDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTASITGGWVALYDCLRWMEARQMASVSKVLKDHVAAISCGIHDGQPVIDLDYLE
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
DSAAGTDANFVMTGKGGIVEIQGTAEGEPFSEDQFAELMGLAKKGIQRLVSLQQMAVA
CCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968