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The map label for this gene is lepA

Identifier: 13473815

GI number: 13473815

Start: 3605903

End: 3607708

Strand: Direct

Name: lepA

Synonym: mlr4536

Alternate gene names: 13473815

Gene position: 3605903-3607708 (Clockwise)

Preceding gene: 13473809

Following gene: 13473816

Centisome position: 51.25

GC content: 64.01

Gene sequence:

>1806_bases
ATGAGCACGCCCCTCGACCATATCAGAAATTTCTCCATCGTCGCCCATATCGACCATGGCAAATCCACGCTCGCCGACCG
CCTGATCCAGTCCACCGGCGGGCTGGAGCTGCGCGACATGAAGGAGCAGGTGCTGGACTCGATGGACATCGAGCGCGAGC
GCGGCATCACCATCAAGGCGCAGACCGTGCGGCTGAAATACCGCGCCAACAATGGCGAGGACTATATCCTCAACCTCATC
GACACGCCCGGACACGTCGACTTCGCCTATGAAGTGTCGCGTTCGCTGGCCGCCTGCGAGGGCTCGCTGCTGGTCGTCGA
CGCCTCGCAGGGCGTCGAGGCGCAGACGCTCGCCAATGTCTACCAGGCCATCGACAACAACCACGAGATCGTCGTGGTGC
TGAACAAGGTCGACCTGCCGGCCGCCGAGCCCGAGCGCATCCGCGAGCAGGTCGAGGAGGTGATCGGCATCGACGCTTCC
GGCGCCGTGCTGATCTCGGCCAAGACCGGCCTTGGCATTCCGGATGTCTTGGAGGCCATCGTCCACCAATTGCCGCCGCC
GCGCGAGGGCGATGCGACAGCGCCGCTGAAGGCGATGCTGGTCGACAGCTGGTACGACGCCTATCTCGGCGTCATCGTGC
TGGTGCGCATCATCGACGGCGTGCTGAAGAAGGGCCAGACCATCCGCATGATGGGCACCGGCGCCAAATACCTGGTCGAG
CGCACCGGCGTGTTCACGCCCGCCCGCATCAATGTCGACGAGCTCGGCCCCGGCGAGTTCGGCTTCTTCACCGGCTCGAT
CAAGGAAGTCGCCGACACGCGCGTCGGCGACACCATCACCGAGGACAAGCGCCCCACCGCGCAGGCGCTGCCCGGCTTCA
AGCCGGCGCAGCCGGTGGTGTTCTGCGGCCTGTTCCCGGTCGACGCCGCCGATTTCGAGGATCTGCGCGCCGCCGTCGGC
AAGCTGCGCCTCAACGACGCGTCCTTCTCCTATGAGATGGAAACCAGTGCTGCGCTCGGCTTCGGCTACCGCTGCGGCTT
CCTCGGCTTGCTTCATCTGGAGATCATCCAGGAGCGGCTGGAGCGCGAGTTCAACCTCGACCTGATCGCCACCGCGCCGT
CCGTCGTCTACCGGCTGCTGCTCAATGACGGCTCGGTGAAGGAACTCCACAACCCGGCCGACATGCCCGACGTGGTGAAG
ATCTCCGCCATCGAGGAACCATGGATCCGCGCCACCATCCTCACCCCCGACGACTATCTCGGCGGCATCCTGAAGCTTTG
CCAGGACCGGCGCGGCATCCAGGCCGACCTGTCCTATGTGGGAAAACGCGCCATGCTGACCTACGATCTGCCGCTCAACG
AAGTCGTCTTCGACTTCTACGACCGGCTGAAGTCGATCTCCAAGGGCTACGCCTCGTTCGACTATCACCTGACCGACTAC
CGCGAGGGCGATCTGGTGAAGATGTCGATCCTGGTCAATGAGGAACCGGTCGACGCGCTGTCCATGCTGGTGCACCGCAC
GGCGGCGGAGAAGCGCGGCCGGCAGATGTGCGAGAAGTTGAAGGAGTTGATCCCGCACCACCTCTTCAAGATCCCGATCC
AGGCCGCCATCGGCGGCAAGGTCATCGCCCGCGAAACCATCTCCGCCCTGCGCAAGGACGTGACCGCCAAATGCTACGGC
GGCGACGTCTCGCGCAAACGCAAGCTGCTGGACAAGCAGAAAGAAGGCAAGAAGCGGATGCGCCAGTTCGGGAAGGTCGA
TATCCCGCAGGAGGCGTTTATCCAGGCGCTTAAGATGGGGGATTGA

Upstream 100 bases:

>100_bases
TTGTCGATTTTTTGGCGCCGCTCATCGCTTTGTGATCGACGGCGCCGCCCTTGCGCCGCAAGCCTTGCCGCCGCAGGCCG
CCCCTGCTAAAGCGCGCGAC

Downstream 100 bases:

>100_bases
GGAGCCGGTTGGCGCGCGGAGCGCGCCAAGTCATCGATCCAGTGGATCGATGACAGGCTTCGAAAGCGGGGGAGCTGCCG
GAGGCAGCGGGCCCCCCCGC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MSTPLDHIRNFSIVAHIDHGKSTLADRLIQSTGGLELRDMKEQVLDSMDIERERGITIKAQTVRLKYRANNGEDYILNLI
DTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYQAIDNNHEIVVVLNKVDLPAAEPERIREQVEEVIGIDAS
GAVLISAKTGLGIPDVLEAIVHQLPPPREGDATAPLKAMLVDSWYDAYLGVIVLVRIIDGVLKKGQTIRMMGTGAKYLVE
RTGVFTPARINVDELGPGEFGFFTGSIKEVADTRVGDTITEDKRPTAQALPGFKPAQPVVFCGLFPVDAADFEDLRAAVG
KLRLNDASFSYEMETSAALGFGYRCGFLGLLHLEIIQERLEREFNLDLIATAPSVVYRLLLNDGSVKELHNPADMPDVVK
ISAIEEPWIRATILTPDDYLGGILKLCQDRRGIQADLSYVGKRAMLTYDLPLNEVVFDFYDRLKSISKGYASFDYHLTDY
REGDLVKMSILVNEEPVDALSMLVHRTAAEKRGRQMCEKLKELIPHHLFKIPIQAAIGGKVIARETISALRKDVTAKCYG
GDVSRKRKLLDKQKEGKKRMRQFGKVDIPQEAFIQALKMGD

Sequences:

>Translated_601_residues
MSTPLDHIRNFSIVAHIDHGKSTLADRLIQSTGGLELRDMKEQVLDSMDIERERGITIKAQTVRLKYRANNGEDYILNLI
DTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYQAIDNNHEIVVVLNKVDLPAAEPERIREQVEEVIGIDAS
GAVLISAKTGLGIPDVLEAIVHQLPPPREGDATAPLKAMLVDSWYDAYLGVIVLVRIIDGVLKKGQTIRMMGTGAKYLVE
RTGVFTPARINVDELGPGEFGFFTGSIKEVADTRVGDTITEDKRPTAQALPGFKPAQPVVFCGLFPVDAADFEDLRAAVG
KLRLNDASFSYEMETSAALGFGYRCGFLGLLHLEIIQERLEREFNLDLIATAPSVVYRLLLNDGSVKELHNPADMPDVVK
ISAIEEPWIRATILTPDDYLGGILKLCQDRRGIQADLSYVGKRAMLTYDLPLNEVVFDFYDRLKSISKGYASFDYHLTDY
REGDLVKMSILVNEEPVDALSMLVHRTAAEKRGRQMCEKLKELIPHHLFKIPIQAAIGGKVIARETISALRKDVTAKCYG
GDVSRKRKLLDKQKEGKKRMRQFGKVDIPQEAFIQALKMGD
>Mature_600_residues
STPLDHIRNFSIVAHIDHGKSTLADRLIQSTGGLELRDMKEQVLDSMDIERERGITIKAQTVRLKYRANNGEDYILNLID
TPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYQAIDNNHEIVVVLNKVDLPAAEPERIREQVEEVIGIDASG
AVLISAKTGLGIPDVLEAIVHQLPPPREGDATAPLKAMLVDSWYDAYLGVIVLVRIIDGVLKKGQTIRMMGTGAKYLVER
TGVFTPARINVDELGPGEFGFFTGSIKEVADTRVGDTITEDKRPTAQALPGFKPAQPVVFCGLFPVDAADFEDLRAAVGK
LRLNDASFSYEMETSAALGFGYRCGFLGLLHLEIIQERLEREFNLDLIATAPSVVYRLLLNDGSVKELHNPADMPDVVKI
SAIEEPWIRATILTPDDYLGGILKLCQDRRGIQADLSYVGKRAMLTYDLPLNEVVFDFYDRLKSISKGYASFDYHLTDYR
EGDLVKMSILVNEEPVDALSMLVHRTAAEKRGRQMCEKLKELIPHHLFKIPIQAAIGGKVIARETISALRKDVTAKCYGG
DVSRKRKLLDKQKEGKKRMRQFGKVDIPQEAFIQALKMGD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=604, Percent_Identity=48.0132450331126, Blast_Score=602, Evalue=1e-172,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=48.1203007518797, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI18390331, Length=163, Percent_Identity=36.8098159509202, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI25306283, Length=159, Percent_Identity=42.7672955974843, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=39.5833333333333, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI19923640, Length=159, Percent_Identity=42.7672955974843, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI25306287, Length=159, Percent_Identity=42.7672955974843, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=45.4545454545455, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=45.4545454545455, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=45.4545454545455, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI217272894, Length=161, Percent_Identity=34.7826086956522, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI217272892, Length=161, Percent_Identity=34.7826086956522, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI94966752, Length=97, Percent_Identity=41.2371134020619, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI53729339, Length=224, Percent_Identity=29.0178571428571, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI53729337, Length=224, Percent_Identity=29.0178571428571, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=56.7114093959732, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=517, Percent_Identity=28.6266924564797, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI1790835, Length=173, Percent_Identity=32.3699421965318, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789738, Length=163, Percent_Identity=35.5828220858896, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1789559, Length=236, Percent_Identity=29.2372881355932, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=616, Percent_Identity=38.474025974026, Blast_Score=444, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17556745, Length=495, Percent_Identity=26.4646464646465, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71988811, Length=138, Percent_Identity=39.1304347826087, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71988819, Length=138, Percent_Identity=39.1304347826087, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=147, Percent_Identity=36.734693877551, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=36.5384615384615, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=34.5864661654135, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=280, Percent_Identity=28.9285714285714, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994658, Length=230, Percent_Identity=27.3913043478261, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=599, Percent_Identity=44.7412353923205, Blast_Score=527, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=40.9722222222222, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=40.9722222222222, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=183, Percent_Identity=36.0655737704918, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=139, Percent_Identity=36.6906474820144, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=170, Percent_Identity=37.0588235294118, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324761, Length=247, Percent_Identity=29.5546558704453, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322675, Length=147, Percent_Identity=29.2517006802721, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI78706572, Length=607, Percent_Identity=44.6457990115321, Blast_Score=536, Evalue=1e-152,
Organism=Drosophila melanogaster, GI24582462, Length=163, Percent_Identity=36.8098159509202, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=44.5255474452555, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=36.4864864864865, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=36.4864864864865, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=36.4864864864865, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21357743, Length=168, Percent_Identity=35.7142857142857, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221458488, Length=144, Percent_Identity=40.2777777777778, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_RHILO (Q98DV1)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105383.1
- ProteinModelPortal:   Q98DV1
- SMR:   Q98DV1
- GeneID:   1228044
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr4536
- NMPDR:   fig|266835.1.peg.3487
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66578; Mature: 66446

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPLDHIRNFSIVAHIDHGKSTLADRLIQSTGGLELRDMKEQVLDSMDIERERGITIKA
CCCCHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHCCCEEEE
QTVRLKYRANNGEDYILNLIDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANV
EEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
YQAIDNNHEIVVVLNKVDLPAAEPERIREQVEEVIGIDASGAVLISAKTGLGIPDVLEAI
HHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
VHQLPPPREGDATAPLKAMLVDSWYDAYLGVIVLVRIIDGVLKKGQTIRMMGTGAKYLVE
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHH
RTGVFTPARINVDELGPGEFGFFTGSIKEVADTRVGDTITEDKRPTAQALPGFKPAQPVV
HCCCCCCCCCCHHCCCCCCCCEECCCHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCEE
FCGLFPVDAADFEDLRAAVGKLRLNDASFSYEMETSAALGFGYRCGFLGLLHLEIIQERL
EEEECCCCCCCHHHHHHHHHHEEECCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHHH
EREFNLDLIATAPSVVYRLLLNDGSVKELHNPADMPDVVKISAIEEPWIRATILTPDDYL
HHHCCCEEEECCHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEECCCCCEEEEEECCHHHH
GGILKLCQDRRGIQADLSYVGKRAMLTYDLPLNEVVFDFYDRLKSISKGYASFDYHLTDY
HHHHHHHHHCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
REGDLVKMSILVNEEPVDALSMLVHRTAAEKRGRQMCEKLKELIPHHLFKIPIQAAIGGK
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCH
VIARETISALRKDVTAKCYGGDVSRKRKLLDKQKEGKKRMRQFGKVDIPQEAFIQALKMG
HHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
D
C
>Mature Secondary Structure 
STPLDHIRNFSIVAHIDHGKSTLADRLIQSTGGLELRDMKEQVLDSMDIERERGITIKA
CCCHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHCCCEEEE
QTVRLKYRANNGEDYILNLIDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANV
EEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
YQAIDNNHEIVVVLNKVDLPAAEPERIREQVEEVIGIDASGAVLISAKTGLGIPDVLEAI
HHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
VHQLPPPREGDATAPLKAMLVDSWYDAYLGVIVLVRIIDGVLKKGQTIRMMGTGAKYLVE
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHH
RTGVFTPARINVDELGPGEFGFFTGSIKEVADTRVGDTITEDKRPTAQALPGFKPAQPVV
HCCCCCCCCCCHHCCCCCCCCEECCCHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCEE
FCGLFPVDAADFEDLRAAVGKLRLNDASFSYEMETSAALGFGYRCGFLGLLHLEIIQERL
EEEECCCCCCCHHHHHHHHHHEEECCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHHH
EREFNLDLIATAPSVVYRLLLNDGSVKELHNPADMPDVVKISAIEEPWIRATILTPDDYL
HHHCCCEEEECCHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEECCCCCEEEEEECCHHHH
GGILKLCQDRRGIQADLSYVGKRAMLTYDLPLNEVVFDFYDRLKSISKGYASFDYHLTDY
HHHHHHHHHCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
REGDLVKMSILVNEEPVDALSMLVHRTAAEKRGRQMCEKLKELIPHHLFKIPIQAAIGGK
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCH
VIARETISALRKDVTAKCYGGDVSRKRKLLDKQKEGKKRMRQFGKVDIPQEAFIQALKMG
HHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11214968