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The map label for this gene is ahpF [C]

Identifier: 13473749

GI number: 13473749

Start: 3536929

End: 3538368

Strand: Direct

Name: ahpF [C]

Synonym: mlr4448

Alternate gene names: 13473749

Gene position: 3536929-3538368 (Clockwise)

Preceding gene: 13473748

Following gene: 13473752

Centisome position: 50.27

GC content: 65.62

Gene sequence:

>1440_bases
ATGACCGACCTCAAGGGGTTCGAGGAGCGCAAGGGCTTGGCACGGCCGCTCGGCGTGCGCCACAAGGCCGACCCCGAACG
CGAGACCTGGTCGGAATCGCGGGCGCGCGAGACGCGCGAGCTCGGCGCTTCGGAGCAGCCCTATTGCCTGGTCATCGGCG
GTGGCCAGGGCGGCATCATGCTGGGCGCGCGGCTGCGGCAGCTCGGCGTGCCGACCATCGTCATCGAGAAGAATGCGCGC
CCGGGCGATTCCTGGCGCAACCGCTACCGCACGCTCGTGCTGCACGATCCGGTCTGGTACGACCATCTGCCCTACATTCC
GTTTCCGGAGAACTGGCCGGTCTTCACGCCCAAGGACAAGATGGGCGACTGGCTGGAAATGTACACGCGCGTCATGGAGC
TCAACTATTGGGTGGCCACCAAATGCCTCAGCGCCTCCTATGACGAGGGGCGGAAGGAGTGGACCGTCGTGGTCGACCGC
GTTGGCCGGCAGATCACGCTGAAGCCGAAACATGTCGTCTTCGCCACCGGCGCCTACGGCCCGCCGCGAAAGATCGACCT
GCCCGGTGCCGCAGCGTTCAAGGGCGAGCTGCTGCATTCCAGCCAGTATGCAAGCGGCGACAAATTCCGCGGCAAGAAGG
TCGCCGTCATCGGCGCCGCCAGCTCCGGGCATGATGTCTGCGTCGACCTCTGGGAAAGCGGCGCCGACGTCACCATGATC
CAGCGCTCGCCGACCACCGTGGTCAAGTCGGACACGCTGATGGAAGTCGGCTTCGAGATCTTCTCGGAGGGCGCGCTGGC
GCGCGGCATCACCACCGAGAAGGCCGACATGATCGTCGCCTCGACGCCCTTCGCGCTGGTGCCCAAGGGCCAGCGCGCGC
TCTACGAGGTGATCAAGGCACGTGACGCGGCCTTCTACGAGCGCCTCAGCGCCTCGGGCTTCGCCATCGATTTCGGCGAC
GACGAGACCGGCCTCTTGATGAAGGCCTATCGCACCGGCTCCGGCTTCTACATCGATGTCGGCGCCTCCGACCTGATCAT
CGACGGCAAGATCGGCATCCGCAGCGGCGTCGCCATCAAGGCGCTGACGGCGAAAGGTATCCTGTTCGAGGACGGCAGCG
AGCTCGAGGTCGACGCCATCATCGCCTGCACCGGCTACCAGTCGATGAACGAGAATGTCGCGGCACTGGTCTCGCGCGAA
GTCGCCGACAAGGTCGGCCCCTGCTGGGGCCTCGGCTCCGGCGTCAAGGGCGATCCCGGCCCCTGGCAAGGCGAGCTGCG
CAACATGTGGAAGCCGACGGCGCAGGAAGCGCTGTGGTTCCACGGTGGCAATCTGGCGCTGTCGCGGTTTTATTCGAAAT
ATGTGGCGCTGCAGATCAAGGCACGCATGGAGGGGATTGCCACGCCGGTTTACGGGAAGCCGAGCAATTCGAGACGATAG

Upstream 100 bases:

>100_bases
AGGCACGATCGAAGCCTGGTTCACCTTCGAGACCGCGGTAGCCTCGGGACAAGGCATCCTGCGGCTGCGCGACGGCCGCT
GCCGCACCCTGTTCACGGCG

Downstream 100 bases:

>100_bases
CATCCCGTGAGTTCGTCATCCTAGGGCGAAGCAAGGAGCGAAGCGACGCGGCGCAGACCCTAGGATCCATGCCGTGCCAT
TCATCACATGCGAATGCGGA

Product: hypothetical protein

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTDLKGFEERKGLARPLGVRHKADPERETWSESRARETRELGASEQPYCLVIGGGQGGIMLGARLRQLGVPTIVIEKNAR
PGDSWRNRYRTLVLHDPVWYDHLPYIPFPENWPVFTPKDKMGDWLEMYTRVMELNYWVATKCLSASYDEGRKEWTVVVDR
VGRQITLKPKHVVFATGAYGPPRKIDLPGAAAFKGELLHSSQYASGDKFRGKKVAVIGAASSGHDVCVDLWESGADVTMI
QRSPTTVVKSDTLMEVGFEIFSEGALARGITTEKADMIVASTPFALVPKGQRALYEVIKARDAAFYERLSASGFAIDFGD
DETGLLMKAYRTGSGFYIDVGASDLIIDGKIGIRSGVAIKALTAKGILFEDGSELEVDAIIACTGYQSMNENVAALVSRE
VADKVGPCWGLGSGVKGDPGPWQGELRNMWKPTAQEALWFHGGNLALSRFYSKYVALQIKARMEGIATPVYGKPSNSRR

Sequences:

>Translated_479_residues
MTDLKGFEERKGLARPLGVRHKADPERETWSESRARETRELGASEQPYCLVIGGGQGGIMLGARLRQLGVPTIVIEKNAR
PGDSWRNRYRTLVLHDPVWYDHLPYIPFPENWPVFTPKDKMGDWLEMYTRVMELNYWVATKCLSASYDEGRKEWTVVVDR
VGRQITLKPKHVVFATGAYGPPRKIDLPGAAAFKGELLHSSQYASGDKFRGKKVAVIGAASSGHDVCVDLWESGADVTMI
QRSPTTVVKSDTLMEVGFEIFSEGALARGITTEKADMIVASTPFALVPKGQRALYEVIKARDAAFYERLSASGFAIDFGD
DETGLLMKAYRTGSGFYIDVGASDLIIDGKIGIRSGVAIKALTAKGILFEDGSELEVDAIIACTGYQSMNENVAALVSRE
VADKVGPCWGLGSGVKGDPGPWQGELRNMWKPTAQEALWFHGGNLALSRFYSKYVALQIKARMEGIATPVYGKPSNSRR
>Mature_478_residues
TDLKGFEERKGLARPLGVRHKADPERETWSESRARETRELGASEQPYCLVIGGGQGGIMLGARLRQLGVPTIVIEKNARP
GDSWRNRYRTLVLHDPVWYDHLPYIPFPENWPVFTPKDKMGDWLEMYTRVMELNYWVATKCLSASYDEGRKEWTVVVDRV
GRQITLKPKHVVFATGAYGPPRKIDLPGAAAFKGELLHSSQYASGDKFRGKKVAVIGAASSGHDVCVDLWESGADVTMIQ
RSPTTVVKSDTLMEVGFEIFSEGALARGITTEKADMIVASTPFALVPKGQRALYEVIKARDAAFYERLSASGFAIDFGDD
ETGLLMKAYRTGSGFYIDVGASDLIIDGKIGIRSGVAIKALTAKGILFEDGSELEVDAIIACTGYQSMNENVAALVSREV
ADKVGPCWGLGSGVKGDPGPWQGELRNMWKPTAQEALWFHGGNLALSRFYSKYVALQIKARMEGIATPVYGKPSNSRR

Specific function: Serves To Protect The Cell Against DNA Damage By Alkyl Hydroperoxides. It Can Use Either NADH Or NADPH As Electron Donor For Direct Reduction Of Redox Dyes Or Of Alkyl Hydroperoxides When Combined With The Ahpc Protein. [C]

COG id: COG2072

COG function: function code P; Predicted flavoprotein involved in K+ transport

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4503757, Length=366, Percent_Identity=22.1311475409836, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI4503759, Length=289, Percent_Identity=24.2214532871972, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4503755, Length=366, Percent_Identity=22.6775956284153, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR000103 [H]

Pfam domain/function: PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 52669; Mature: 52538

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLKGFEERKGLARPLGVRHKADPERETWSESRARETRELGASEQPYCLVIGGGQGGIM
CCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEE
LGARLRQLGVPTIVIEKNARPGDSWRNRYRTLVLHDPVWYDHLPYIPFPENWPVFTPKDK
HHHHHHHCCCCEEEEECCCCCCHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCEECCHHH
MGDWLEMYTRVMELNYWVATKCLSASYDEGRKEWTVVVDRVGRQITLKPKHVVFATGAYG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEEECCCCEEEECCCEEEEEECCCC
PPRKIDLPGAAAFKGELLHSSQYASGDKFRGKKVAVIGAASSGHDVCVDLWESGADVTMI
CCCEECCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEHEECCCCCCEEEE
QRSPTTVVKSDTLMEVGFEIFSEGALARGITTEKADMIVASTPFALVPKGQRALYEVIKA
ECCCCEEEECCHHHHHHHHHHCCCCHHCCCCCCCCCEEEECCCEEECCCCHHHHHHHHHH
RDAAFYERLSASGFAIDFGDDETGLLMKAYRTGSGFYIDVGASDLIIDGKIGIRSGVAIK
HHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCEEEEECCCEEEEECCCCCCCCCEEE
ALTAKGILFEDGSELEVDAIIACTGYQSMNENVAALVSREVADKVGPCWGLGSGVKGDPG
EEEECCEEEECCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PWQGELRNMWKPTAQEALWFHGGNLALSRFYSKYVALQIKARMEGIATPVYGKPSNSRR
CCCHHHHHHCCCCHHHEEEEECCCHHHHHHHHHHEEEEEEEHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDLKGFEERKGLARPLGVRHKADPERETWSESRARETRELGASEQPYCLVIGGGQGGIM
CCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEE
LGARLRQLGVPTIVIEKNARPGDSWRNRYRTLVLHDPVWYDHLPYIPFPENWPVFTPKDK
HHHHHHHCCCCEEEEECCCCCCHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCEECCHHH
MGDWLEMYTRVMELNYWVATKCLSASYDEGRKEWTVVVDRVGRQITLKPKHVVFATGAYG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEEECCCCEEEECCCEEEEEECCCC
PPRKIDLPGAAAFKGELLHSSQYASGDKFRGKKVAVIGAASSGHDVCVDLWESGADVTMI
CCCEECCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEHEECCCCCCEEEE
QRSPTTVVKSDTLMEVGFEIFSEGALARGITTEKADMIVASTPFALVPKGQRALYEVIKA
ECCCCEEEECCHHHHHHHHHHCCCCHHCCCCCCCCCEEEECCCEEECCCCHHHHHHHHHH
RDAAFYERLSASGFAIDFGDDETGLLMKAYRTGSGFYIDVGASDLIIDGKIGIRSGVAIK
HHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCEEEEECCCEEEEECCCCCCCCCEEE
ALTAKGILFEDGSELEVDAIIACTGYQSMNENVAALVSREVADKVGPCWGLGSGVKGDPG
EEEECCEEEECCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PWQGELRNMWKPTAQEALWFHGGNLALSRFYSKYVALQIKARMEGIATPVYGKPSNSRR
CCCHHHHHHCCCCHHHEEEEECCCHHHHHHHHHHEEEEEEEHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA