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The map label for this gene is leuB

Identifier: 13473709

GI number: 13473709

Start: 3490632

End: 3491735

Strand: Reverse

Name: leuB

Synonym: mll4399

Alternate gene names: 13473709

Gene position: 3491735-3490632 (Counterclockwise)

Preceding gene: 13473710

Following gene: 13473703

Centisome position: 49.63

GC content: 65.31

Gene sequence:

>1104_bases
ATGGCAACGAAGCATCTTTTCCTGCTCCCCGGCGACGGCATCGGCCCCGAGGCCATGGCCGAGGTCAAGAAGCTGATATC
GGCCATGAACGAAAAGCTCGGCAGCGGCTTCGCCACCGATGAGGGCCTGGTCGGCGGCTGCGCCTACGACGCGCATGGCG
CGGCGATTTCCGATGCCGACATGGCCAAGGCGATGGCGGCGGACGCGGTGCTGTTCGGCGCGGTCGGCGGACCGAAATGG
GATGCGGTGCCTTACGAGGTGCGCCCCGAGGCCGGCCTGCTGCGGCTGCGCAAGGACATGGAGCTGTTCGCCAATCTGCG
CCCCGCCATCTGCTATCCGGCGCTCGCCGCATCCTCCTCGCTCAAGCAGGAGGTGGTCGAAGGGCTCGACATCCTCATCG
TGCGCGAGCTGACTGGCGGCGTCTATTTCGGCGAGCCCAAGCAGATCATCGATCTCGGCAACGGCCAGAAGCGCGGCATC
GACACGCAGGTCTATGACACGTTCGAGATCGAGCGCATTTCGGGCGTCGCCTTCGAGCTCGCGCGCACCCGCAAGAACCA
CGTCACCTCGATGGAAAAGCGCAACGTCATGAAGTCGGGCGTGCTGTGGAACGAGGTGGTCACCCAGACGCACAAGGCGC
GCTACGCCGACGTCAAGCTCGACCACATGCTGGCCGATGCCGGCGGCATGCAGTTGGTGCGCTGGCCAAAACAGTTCGAC
GTCATCGTCACCGACAATCTGTTCGGCGACATGCTGTCCGACATCGCCGCCATGCTGACCGGCTCGATCGGCATGCTGCC
GTCCGCCTCGCTCGGCGCGCCCGACGTCAAGACCAAGAAGCGCAAGGCGCTCTACGAGCCCGTGCACGGCTCGGCGCCCG
ACATCGCCGGCAAGGGCATCGCCAACCCGATCGCCATGATCGCTTCCTTCGCCATGTGCCTGCGCTATTCCTTCGGCATG
GTCGATGAGGCCGACAGGCTGGAAGGCGCGATCGCCGCCGTGCTCGATCAGGGCCTGCGGACGAAGGACATTTTCTCGCC
GGGCATGACCGAGGTCGGCACGGTCGAGATGGGCGACGCGATCATCGCCAAGTTCCTGGGTTAA

Upstream 100 bases:

>100_bases
TCTCACATCGGTGATCGTGGTTCCGAACCGCAGGCTTGCGCCGCAAGCGCTTGGCGTTTAGCAAGGCCGCGGTTTCAAAT
CTTTCCAGGACGGTTTCTCC

Downstream 100 bases:

>100_bases
CCCGCTCACTCAGAGCGTTTCACCGTTTCATGGAAACGGCGAACCGATCTAACTCTTGTTTTGACGCAATTCCGGACGGA
AAACCGCTCACACTTTTCCT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MATKHLFLLPGDGIGPEAMAEVKKLISAMNEKLGSGFATDEGLVGGCAYDAHGAAISDADMAKAMAADAVLFGAVGGPKW
DAVPYEVRPEAGLLRLRKDMELFANLRPAICYPALAASSSLKQEVVEGLDILIVRELTGGVYFGEPKQIIDLGNGQKRGI
DTQVYDTFEIERISGVAFELARTRKNHVTSMEKRNVMKSGVLWNEVVTQTHKARYADVKLDHMLADAGGMQLVRWPKQFD
VIVTDNLFGDMLSDIAAMLTGSIGMLPSASLGAPDVKTKKRKALYEPVHGSAPDIAGKGIANPIAMIASFAMCLRYSFGM
VDEADRLEGAIAAVLDQGLRTKDIFSPGMTEVGTVEMGDAIIAKFLG

Sequences:

>Translated_367_residues
MATKHLFLLPGDGIGPEAMAEVKKLISAMNEKLGSGFATDEGLVGGCAYDAHGAAISDADMAKAMAADAVLFGAVGGPKW
DAVPYEVRPEAGLLRLRKDMELFANLRPAICYPALAASSSLKQEVVEGLDILIVRELTGGVYFGEPKQIIDLGNGQKRGI
DTQVYDTFEIERISGVAFELARTRKNHVTSMEKRNVMKSGVLWNEVVTQTHKARYADVKLDHMLADAGGMQLVRWPKQFD
VIVTDNLFGDMLSDIAAMLTGSIGMLPSASLGAPDVKTKKRKALYEPVHGSAPDIAGKGIANPIAMIASFAMCLRYSFGM
VDEADRLEGAIAAVLDQGLRTKDIFSPGMTEVGTVEMGDAIIAKFLG
>Mature_366_residues
ATKHLFLLPGDGIGPEAMAEVKKLISAMNEKLGSGFATDEGLVGGCAYDAHGAAISDADMAKAMAADAVLFGAVGGPKWD
AVPYEVRPEAGLLRLRKDMELFANLRPAICYPALAASSSLKQEVVEGLDILIVRELTGGVYFGEPKQIIDLGNGQKRGID
TQVYDTFEIERISGVAFELARTRKNHVTSMEKRNVMKSGVLWNEVVTQTHKARYADVKLDHMLADAGGMQLVRWPKQFDV
IVTDNLFGDMLSDIAAMLTGSIGMLPSASLGAPDVKTKKRKALYEPVHGSAPDIAGKGIANPIAMIASFAMCLRYSFGMV
DEADRLEGAIAAVLDQGLRTKDIFSPGMTEVGTVEMGDAIIAKFLG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=341, Percent_Identity=32.8445747800587, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI28178821, Length=324, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI28178816, Length=324, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4758582, Length=350, Percent_Identity=28, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI28178838, Length=336, Percent_Identity=27.6785714285714, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI28178819, Length=149, Percent_Identity=33.5570469798658, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI87081683, Length=365, Percent_Identity=50.1369863013699, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1788101, Length=352, Percent_Identity=34.375, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1787381, Length=312, Percent_Identity=27.2435897435897, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71986051, Length=356, Percent_Identity=30.6179775280899, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=344, Percent_Identity=30.8139534883721, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25144293, Length=345, Percent_Identity=28.695652173913, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=362, Percent_Identity=29.5580110497238, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=366, Percent_Identity=40.4371584699454, Blast_Score=245, Evalue=9e-66,
Organism=Saccharomyces cerevisiae, GI6322097, Length=349, Percent_Identity=34.9570200573066, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6324709, Length=346, Percent_Identity=31.2138728323699, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6324291, Length=348, Percent_Identity=27.0114942528736, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24643268, Length=342, Percent_Identity=32.7485380116959, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24643270, Length=342, Percent_Identity=32.7485380116959, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24661184, Length=348, Percent_Identity=29.8850574712644, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161078635, Length=343, Percent_Identity=27.4052478134111, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI161078633, Length=343, Percent_Identity=27.4052478134111, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24650122, Length=343, Percent_Identity=27.4052478134111, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI161078637, Length=343, Percent_Identity=27.4052478134111, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI161078639, Length=343, Percent_Identity=27.4052478134111, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281362242, Length=347, Percent_Identity=25.3602305475504, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648872, Length=347, Percent_Identity=25.3602305475504, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_RHILO (Q98E57)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105277.1
- ProteinModelPortal:   Q98E57
- SMR:   Q98E57
- GeneID:   1227938
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll4399
- NMPDR:   fig|266835.1.peg.3381
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 39283; Mature: 39152

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 97-97 BINDING 107-107 BINDING 135-135 BINDING 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKHLFLLPGDGIGPEAMAEVKKLISAMNEKLGSGFATDEGLVGGCAYDAHGAAISDAD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECEEECCCCCCCCHHH
MAKAMAADAVLFGAVGGPKWDAVPYEVRPEAGLLRLRKDMELFANLRPAICYPALAASSS
HHHHHHHHHHHEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKQEVVEGLDILIVRELTGGVYFGEPKQIIDLGNGQKRGIDTQVYDTFEIERISGVAFEL
HHHHHHCCHHEEEEEECCCCEEECCCHHEEECCCCCCCCCCCHHHCCEEHHHHHHHHHHH
ARTRKNHVTSMEKRNVMKSGVLWNEVVTQTHKARYADVKLDHMLADAGGMQLVRWPKQFD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCC
VIVTDNLFGDMLSDIAAMLTGSIGMLPSASLGAPDVKTKKRKALYEPVHGSAPDIAGKGI
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
ANPIAMIASFAMCLRYSFGMVDEADRLEGAIAAVLDQGLRTKDIFSPGMTEVGTVEMGDA
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEHHHH
IIAKFLG
HHHHHCC
>Mature Secondary Structure 
ATKHLFLLPGDGIGPEAMAEVKKLISAMNEKLGSGFATDEGLVGGCAYDAHGAAISDAD
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECEEECCCCCCCCHHH
MAKAMAADAVLFGAVGGPKWDAVPYEVRPEAGLLRLRKDMELFANLRPAICYPALAASSS
HHHHHHHHHHHEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKQEVVEGLDILIVRELTGGVYFGEPKQIIDLGNGQKRGIDTQVYDTFEIERISGVAFEL
HHHHHHCCHHEEEEEECCCCEEECCCHHEEECCCCCCCCCCCHHHCCEEHHHHHHHHHHH
ARTRKNHVTSMEKRNVMKSGVLWNEVVTQTHKARYADVKLDHMLADAGGMQLVRWPKQFD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCC
VIVTDNLFGDMLSDIAAMLTGSIGMLPSASLGAPDVKTKKRKALYEPVHGSAPDIAGKGI
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
ANPIAMIASFAMCLRYSFGMVDEADRLEGAIAAVLDQGLRTKDIFSPGMTEVGTVEMGDA
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEHHHH
IIAKFLG
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968