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The map label for this gene is argG

Identifier: 13473685

GI number: 13473685

Start: 3469294

End: 3470517

Strand: Direct

Name: argG

Synonym: mlr4366

Alternate gene names: 13473685

Gene position: 3469294-3470517 (Clockwise)

Preceding gene: 13473682

Following gene: 13473686

Centisome position: 49.31

GC content: 63.64

Gene sequence:

>1224_bases
ATGTCCAAGACCAAAGACGTCAAGAAAGTCGTGCTCGCCTATTCCGGCGGCCTCGACACATCCATCATCCTGAAATGGCT
GCAGACCGAACTCGGCGCCGAAGTCGTCACCTTCACCGCCGATCTCGGCCAGGGTGGCGAGTTGGAGCCGGCACGCAAGA
AGGCCGAGATGATGGGCATCAAGGACATCCGCATCGTCGATGTGCGGGAGGAATTCGTCGCCGACTTCGTCTTCCCGATG
TTCCGTGCCAACACCGTCTATGAAGGCACCTATCTGCTCGGCACCTCGATCGCGCGGCCGCTGATCTCCAAGCACCTTGT
CGACATCGCCAGGGAGACCGGCGCCGATGCGATCGCCCATGGCGCCACCGGCAAGGGCAACGACCAGGTCCGCTTCGAGC
TGTCGGCCTATGCGCTGAACCCCGATATCAAAGTCATCGCGCCCTGGCGCGACTGGTCGTTCAAGTCGCGCACCGACCTG
ATCAACTTCGCCGAGCAGCACCAGATCCCGGTGGCCAAGGACAAAAGGGGCGAGGCGCCGTTCTCGGTCGACGCCAACCT
GCTGCACTCCTCTTCCGAGGGCAAGGTGCTGGAAGATCCGTGGAGCGAGCCGCCGGAATTCGTCCACCAGCGCACGGTCT
CGCCGATGGACGCGCCGGACAAGGTAACCGAGATCGAGATCGAGTTCCTGAAGGGCGACCCGATCGCGCTCAACGGCAAG
AAGCTGTCGCCGGCGACCATGCTGGCCGCCCTCAACGATCTTGGCCGCGACAACGGCATCGGCCGGCTCGACCTGGTCGA
GAACCGTTTCGTCGGCATGAAATCGCGCGGCGTCTACGAGACGCCAGGCGGCGCCATCCTGATCGTGGCCCACCGCGCCA
TCGAATCGATCACGCTCGACCGCGGCGCCGCGCACCTCAAGGACGAGTTCATGCCGCGCTACGCCGAGCTCATCTACAAC
GGCTTCTGGTTCTCGCCCGAGCGCCTGATGCTGCAGGCGATGATCGACAAGAGCCAGGAAGACGTCGAAGGCACGGTACG
GTTGAAGCTCTACAAGGGCAATGTCATGGTCACCGGCCGCAAGTCGAAGAAGACGCTCTATTCCGACGCGCTGGTCACCT
TCGAGGACGATCGCGGCGCCTACGACCAGAAGGACGCCGCCGGCTTCATCCGCCTCAATGCGCTGAGGCTGCGCACGCTG
GCTGCGCGCAACCGCAAGGGCTGA

Upstream 100 bases:

>100_bases
CTGGCGCCGCGCCTGCTTGCTCGCTTTGCCGTTCCTGCTAAAAGGCGCTCCGACAATGCGTGACGCCCTTGCCCAGGCGT
CCGCCACCGACGGAGCAGAA

Downstream 100 bases:

>100_bases
TATCCAGACTGCGGCCTGGCGCTTTGCCAATCAATGTTCGAGAAACCCGGCGGAGACGCCGGGTTTTTATTTGGTACCGC
TAATATAGATAAAATGAAGC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSKTKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGGELEPARKKAEMMGIKDIRIVDVREEFVADFVFPM
FRANTVYEGTYLLGTSIARPLISKHLVDIARETGADAIAHGATGKGNDQVRFELSAYALNPDIKVIAPWRDWSFKSRTDL
INFAEQHQIPVAKDKRGEAPFSVDANLLHSSSEGKVLEDPWSEPPEFVHQRTVSPMDAPDKVTEIEIEFLKGDPIALNGK
KLSPATMLAALNDLGRDNGIGRLDLVENRFVGMKSRGVYETPGGAILIVAHRAIESITLDRGAAHLKDEFMPRYAELIYN
GFWFSPERLMLQAMIDKSQEDVEGTVRLKLYKGNVMVTGRKSKKTLYSDALVTFEDDRGAYDQKDAAGFIRLNALRLRTL
AARNRKG

Sequences:

>Translated_407_residues
MSKTKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGGELEPARKKAEMMGIKDIRIVDVREEFVADFVFPM
FRANTVYEGTYLLGTSIARPLISKHLVDIARETGADAIAHGATGKGNDQVRFELSAYALNPDIKVIAPWRDWSFKSRTDL
INFAEQHQIPVAKDKRGEAPFSVDANLLHSSSEGKVLEDPWSEPPEFVHQRTVSPMDAPDKVTEIEIEFLKGDPIALNGK
KLSPATMLAALNDLGRDNGIGRLDLVENRFVGMKSRGVYETPGGAILIVAHRAIESITLDRGAAHLKDEFMPRYAELIYN
GFWFSPERLMLQAMIDKSQEDVEGTVRLKLYKGNVMVTGRKSKKTLYSDALVTFEDDRGAYDQKDAAGFIRLNALRLRTL
AARNRKG
>Mature_406_residues
SKTKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGGELEPARKKAEMMGIKDIRIVDVREEFVADFVFPMF
RANTVYEGTYLLGTSIARPLISKHLVDIARETGADAIAHGATGKGNDQVRFELSAYALNPDIKVIAPWRDWSFKSRTDLI
NFAEQHQIPVAKDKRGEAPFSVDANLLHSSSEGKVLEDPWSEPPEFVHQRTVSPMDAPDKVTEIEIEFLKGDPIALNGKK
LSPATMLAALNDLGRDNGIGRLDLVENRFVGMKSRGVYETPGGAILIVAHRAIESITLDRGAAHLKDEFMPRYAELIYNG
FWFSPERLMLQAMIDKSQEDVEGTVRLKLYKGNVMVTGRKSKKTLYSDALVTFEDDRGAYDQKDAAGFIRLNALRLRTLA
ARNRKG

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=398, Percent_Identity=47.2361809045226, Blast_Score=354, Evalue=7e-98,
Organism=Homo sapiens, GI16950633, Length=398, Percent_Identity=47.2361809045226, Blast_Score=354, Evalue=7e-98,
Organism=Escherichia coli, GI1789563, Length=410, Percent_Identity=29.5121951219512, Blast_Score=147, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324514, Length=404, Percent_Identity=45.049504950495, Blast_Score=349, Evalue=5e-97,
Organism=Drosophila melanogaster, GI21358151, Length=402, Percent_Identity=48.0099502487562, Blast_Score=355, Evalue=4e-98,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_RHILO (Q98E81)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105253.1
- ProteinModelPortal:   Q98E81
- SMR:   Q98E81
- GeneID:   1227914
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr4366
- NMPDR:   fig|266835.1.peg.3357
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- ProtClustDB:   PRK00509
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45230; Mature: 45099

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 40-40 BINDING 91-91 BINDING 96-96 BINDING 121-121 BINDING 123-123 BINDING 127-127 BINDING 127-127 BINDING 128-128 BINDING 131-131 BINDING 182-182 BINDING 191-191 BINDING 267-267 BINDING 279-279

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGGELEPARKKAEMMGI
CCCCHHHHHHHEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHCCC
KDIRIVDVREEFVADFVFPMFRANTVYEGTYLLGTSIARPLISKHLVDIARETGADAIAH
CCEEEEEHHHHHHHHHHHHHHHCCEEECCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHC
GATGKGNDQVRFELSAYALNPDIKVIAPWRDWSFKSRTDLINFAEQHQIPVAKDKRGEAP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
FSVDANLLHSSSEGKVLEDPWSEPPEFVHQRTVSPMDAPDKVTEIEIEFLKGDPIALNGK
CCCCHHHEECCCCCCEECCCCCCCHHHHHHHCCCCCCCCCCEEEEEEEEECCCCEEECCC
KLSPATMLAALNDLGRDNGIGRLDLVENRFVGMKSRGVYETPGGAILIVAHRAIESITLD
CCCHHHHHHHHHHHCCCCCCCEEEEHHCCCCCCCCCCCEECCCCEEEEEEEHHHHHHECC
RGAAHLKDEFMPRYAELIYNGFWFSPERLMLQAMIDKSQEDVEGTVRLKLYKGNVMVTGR
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCEEEECC
KSKKTLYSDALVTFEDDRGAYDQKDAAGFIRLNALRLRTLAARNRKG
CCCHHEECCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKTKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGGELEPARKKAEMMGI
CCCHHHHHHHEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHCCC
KDIRIVDVREEFVADFVFPMFRANTVYEGTYLLGTSIARPLISKHLVDIARETGADAIAH
CCEEEEEHHHHHHHHHHHHHHHCCEEECCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHC
GATGKGNDQVRFELSAYALNPDIKVIAPWRDWSFKSRTDLINFAEQHQIPVAKDKRGEAP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
FSVDANLLHSSSEGKVLEDPWSEPPEFVHQRTVSPMDAPDKVTEIEIEFLKGDPIALNGK
CCCCHHHEECCCCCCEECCCCCCCHHHHHHHCCCCCCCCCCEEEEEEEEECCCCEEECCC
KLSPATMLAALNDLGRDNGIGRLDLVENRFVGMKSRGVYETPGGAILIVAHRAIESITLD
CCCHHHHHHHHHHHCCCCCCCEEEEHHCCCCCCCCCCCEECCCCEEEEEEEHHHHHHECC
RGAAHLKDEFMPRYAELIYNGFWFSPERLMLQAMIDKSQEDVEGTVRLKLYKGNVMVTGR
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCEEEECC
KSKKTLYSDALVTFEDDRGAYDQKDAAGFIRLNALRLRTLAARNRKG
CCCHHEECCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968