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The map label for this gene is clcA [H]

Identifier: 13473678

GI number: 13473678

Start: 3461884

End: 3463227

Strand: Reverse

Name: clcA [H]

Synonym: mll4356

Alternate gene names: 13473678

Gene position: 3463227-3461884 (Counterclockwise)

Preceding gene: 13473679

Following gene: 13473676

Centisome position: 49.22

GC content: 69.57

Gene sequence:

>1344_bases
ATGGCCGGTACATCGCGAAAATATCCCATGCTGCGGCGCTCGCGGGCGATGTGGGGCTCGCGGCGTGTGTGGCAGCCGCG
GCTGGTGTTCTGGGCCGGCGCGGTCTCGATCGGTGTCATCAGCGTGCTGTTCGCGGTGCTGGCCGACAAGGCGCAGGCGC
TGTTCCATATGATGATCGGCGATGGCGGCGGCCGGCGCTTCTATCTGCCGCTTGCGATCACCCCGCTCGGCTTTGTCCTA
TGCGCCTGGCTCGCCCGCGCCTTCTTCCCGGGTTCGCAAGGCAGCGGCATTCCGCAGGCGATCGCCGCGCGCCATCTGCG
CGATGACGACGATCGCAGCCACCTTCTGTCGCTGCGGCTGGCGGCGGGCAAGATCGCGCTCACCATCGTCGGCCTGTTCT
GCGGCGCTTCCATCGGCCGCGAGGGGCCGACCGTGCAGGTCGGCGCCTCGCTGATGCTGCAGGCGGCGCGCTGGGGCGGC
ATGGCCCAGGCGCGCGGCCTGATCCTGGCCGGCTCGGCCGCAGGCATTGCCGCCGCCTTCAACACGCCGCTGGCCGGCAT
CGTCTTCGCCATCGAGGAGATGGGCCGCACCTATGAGGCGCGCACCAACGGGCTGGTGCTGACGGCGGTGATCCTGGCCG
GCCTTGCCTCGCTCGGCCTGCTCGGCAACTACACCTATTTCGGCGTCTCGAAGGAGACGATCGCGTTTGCCGCCGACTGG
CCGCTGGTGCTTGGTTGCGGCATCATCGGCGGCGGCTTCGGCGCGCTGTTCTCGCTGCTGGCGCTGAAGATCACACGGCG
GATCCGCCGCTGGCACACGCAGCAGCCTTTGGCGCGCGCCTTGCTGGTGGCGGCTGTCTGCGGGCTCGGCGTGGCGGTGA
TCGGCATCGCCTCGGGCGGGCTGACCTTCGGCACCGGCTACGCGCAGGCGCGCGGCGCTGTCGAAGGCACGCCGCTGCCA
TGGTTCTTCTTTGCCGAAAAATTCCTGGCCGGGCTGCTGTCGATGGTCTCGGGCATTCCCGGCGGCATCTTCGCGCCGTC
GCTGGCGGTCGGCGCCGGCATCGGCAGTTCGCTCGGGCTGCTGTTCGGCGCCAGCGCGGGGGCTGCGGCGCTGCTCGGCA
TGGCCGGCTATTTCGCCGGCGTCGTGCAGGCGCCGATGACGGCCTTCGTCATCATCCTGGAAATGACCGGCAATCACGAC
AATGTCATCGCGCTGATGCTGGCCTCGATGCTGGGCTACGGCACGGCGCGCATGATCTCGCACGAGCCGCTCTATCATGC
GCTGTCGCGCGTCTTCATCGCCGAGGCGATCCGGCGGCGGCGGGCGGAGACAGGTCAGGTCTGA

Upstream 100 bases:

>100_bases
CCCGCTCCCCGTCATTTAGCTCGCGTGTGACGGCCTGACCTCGCAGGCTTGATCGACCTCATGCCTGTGCTAGGGCTGGG
CATTCACCGCGAGGACAGAC

Downstream 100 bases:

>100_bases
AAAAGCAAAGGGCCGAGCTTTGCGGCCCGGCCCTTTCGCTTCAAAACGATGTTTTGCCTATTTGCACTTGGCGATCGACG
ACAACGCCGCCGAAATGCCC

Product: chloride channel protein

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MAGTSRKYPMLRRSRAMWGSRRVWQPRLVFWAGAVSIGVISVLFAVLADKAQALFHMMIGDGGGRRFYLPLAITPLGFVL
CAWLARAFFPGSQGSGIPQAIAARHLRDDDDRSHLLSLRLAAGKIALTIVGLFCGASIGREGPTVQVGASLMLQAARWGG
MAQARGLILAGSAAGIAAAFNTPLAGIVFAIEEMGRTYEARTNGLVLTAVILAGLASLGLLGNYTYFGVSKETIAFAADW
PLVLGCGIIGGGFGALFSLLALKITRRIRRWHTQQPLARALLVAAVCGLGVAVIGIASGGLTFGTGYAQARGAVEGTPLP
WFFFAEKFLAGLLSMVSGIPGGIFAPSLAVGAGIGSSLGLLFGASAGAAALLGMAGYFAGVVQAPMTAFVIILEMTGNHD
NVIALMLASMLGYGTARMISHEPLYHALSRVFIAEAIRRRRAETGQV

Sequences:

>Translated_447_residues
MAGTSRKYPMLRRSRAMWGSRRVWQPRLVFWAGAVSIGVISVLFAVLADKAQALFHMMIGDGGGRRFYLPLAITPLGFVL
CAWLARAFFPGSQGSGIPQAIAARHLRDDDDRSHLLSLRLAAGKIALTIVGLFCGASIGREGPTVQVGASLMLQAARWGG
MAQARGLILAGSAAGIAAAFNTPLAGIVFAIEEMGRTYEARTNGLVLTAVILAGLASLGLLGNYTYFGVSKETIAFAADW
PLVLGCGIIGGGFGALFSLLALKITRRIRRWHTQQPLARALLVAAVCGLGVAVIGIASGGLTFGTGYAQARGAVEGTPLP
WFFFAEKFLAGLLSMVSGIPGGIFAPSLAVGAGIGSSLGLLFGASAGAAALLGMAGYFAGVVQAPMTAFVIILEMTGNHD
NVIALMLASMLGYGTARMISHEPLYHALSRVFIAEAIRRRRAETGQV
>Mature_446_residues
AGTSRKYPMLRRSRAMWGSRRVWQPRLVFWAGAVSIGVISVLFAVLADKAQALFHMMIGDGGGRRFYLPLAITPLGFVLC
AWLARAFFPGSQGSGIPQAIAARHLRDDDDRSHLLSLRLAAGKIALTIVGLFCGASIGREGPTVQVGASLMLQAARWGGM
AQARGLILAGSAAGIAAAFNTPLAGIVFAIEEMGRTYEARTNGLVLTAVILAGLASLGLLGNYTYFGVSKETIAFAADWP
LVLGCGIIGGGFGALFSLLALKITRRIRRWHTQQPLARALLVAAVCGLGVAVIGIASGGLTFGTGYAQARGAVEGTPLPW
FFFAEKFLAGLLSMVSGIPGGIFAPSLAVGAGIGSSLGLLFGASAGAAALLGMAGYFAGVVQAPMTAFVIILEMTGNHDN
VIALMLASMLGYGTARMISHEPLYHALSRVFIAEAIRRRRAETGQV

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786350, Length=390, Percent_Identity=29.7435897435897, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87081943, Length=303, Percent_Identity=28.7128712871287, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 46653; Mature: 46521

Theoretical pI: Translated: 11.05; Mature: 11.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTSRKYPMLRRSRAMWGSRRVWQPRLVFWAGAVSIGVISVLFAVLADKAQALFHMMIG
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGGGRRFYLPLAITPLGFVLCAWLARAFFPGSQGSGIPQAIAARHLRDDDDRSHLLSLRL
CCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHH
AAGKIALTIVGLFCGASIGREGPTVQVGASLMLQAARWGGMAQARGLILAGSAAGIAAAF
HHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHCCCHHHHCCEEEECCCCCHHHHH
NTPLAGIVFAIEEMGRTYEARTNGLVLTAVILAGLASLGLLGNYTYFGVSKETIAFAADW
CCCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHEEEECCC
PLVLGCGIIGGGFGALFSLLALKITRRIRRWHTQQPLARALLVAAVCGLGVAVIGIASGG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LTFGTGYAQARGAVEGTPLPWFFFAEKFLAGLLSMVSGIPGGIFAPSLAVGAGIGSSLGL
CEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHH
LFGASAGAAALLGMAGYFAGVVQAPMTAFVIILEMTGNHDNVIALMLASMLGYGTARMIS
HHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHC
HEPLYHALSRVFIAEAIRRRRAETGQV
CCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AGTSRKYPMLRRSRAMWGSRRVWQPRLVFWAGAVSIGVISVLFAVLADKAQALFHMMIG
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGGGRRFYLPLAITPLGFVLCAWLARAFFPGSQGSGIPQAIAARHLRDDDDRSHLLSLRL
CCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHH
AAGKIALTIVGLFCGASIGREGPTVQVGASLMLQAARWGGMAQARGLILAGSAAGIAAAF
HHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHCCCHHHHCCEEEECCCCCHHHHH
NTPLAGIVFAIEEMGRTYEARTNGLVLTAVILAGLASLGLLGNYTYFGVSKETIAFAADW
CCCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHEEEECCC
PLVLGCGIIGGGFGALFSLLALKITRRIRRWHTQQPLARALLVAAVCGLGVAVIGIASGG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LTFGTGYAQARGAVEGTPLPWFFFAEKFLAGLLSMVSGIPGGIFAPSLAVGAGIGSSLGL
CEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHH
LFGASAGAAALLGMAGYFAGVVQAPMTAFVIILEMTGNHDNVIALMLASMLGYGTARMIS
HHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHC
HEPLYHALSRVFIAEAIRRRRAETGQV
CCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA