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The map label for this gene is mdh

Identifier: 13473642

GI number: 13473642

Start: 3430367

End: 3431335

Strand: Reverse

Name: mdh

Synonym: mll4308

Alternate gene names: 13473642

Gene position: 3431335-3430367 (Counterclockwise)

Preceding gene: 13473644

Following gene: 13473641

Centisome position: 48.77

GC content: 63.88

Gene sequence:

>969_bases
ATGGCACGCAACAAGATAGCGCTTATCGGCTCGGGCATGATCGGCGGCACGCTGGCCCACATGATCGGCCTCAAGGACCT
CGGCGACGTGGTGCTGTTCGATATTGCCGAGGGTATTCCGCAAGGCAAGGGGCTCGATATCGCGCAGTCGTCGCCGGTCG
ATGGTTTCGATTCCCGGCTGACCGGCGTCAACGACTATGCCGGCATCGAGGGTGCCGACGTCTGCATCGTTACAGCCGGC
GTGCCGCGCAAGCCTGGCATGAGCCGCGACGATCTGCTGGGCATCAACCTCAAGGTCATGGAACAGGTCGGCGCCGGTCT
GAAGAAGTATGCGCCCAAGGCCTTCGTCATCTGCATCACCAATCCGCTCGACGCCATGGTGTGGGCGCTGCAGAAGTTTT
CCGGCCTGCCCAAGACCCATGTCGTCGGCATGGCCGGCGTGCTCGACAGCGCGCGCTTCCGCTATTTCCTGGCCGAGGAA
TTCAAGGTCTCGGTCGAGGACGTCACCGCCTTCGTGCTCGGCGGCCACGGCGATTCCATGGTGCCGATGATCCGCTACTC
GACGGTATCGGGCATCCCGCTGCCCGACCTCGTCAAGATGGGCTGGACCTCGAAGGAGAAGCTCGACCAGATCGTCCAGC
GCACCCGTGATGGCGGCGCCGAGATCGTCGGCCTGCTCAAGACCGGCTCGGCCTATTACGCGCCGGCGGCCTCGGCGATT
GCCATGGCCGAATCCTACCTCAAGGACAAGAAGCGCGTGCTGCCCTGCGCTGCCCACCTTTCCGGCCAGTATGGCGTCAA
GGGCACCTATGTCGGCGTCCCCGTGGTGATCGGCGCCGGCGGCGTCGAGCGCATCATCGAGATCGACCTCAACAAGAGCG
AACAGAAAATGTTCGAGAGTTCGGTGGCGACCGTGCAGGGCCTGACCGAGGCCTGCGTCAAGATCGCGCCGCAGCTCGCT
TCGAAGTGA

Upstream 100 bases:

>100_bases
ATCTCGCGGGTTTCCTCGGCATTCCGGCCTCTTCCTCGACGCCGTCATCAAGTCGTCAAAGATTTGGGCGCAGTCACAGA
CAAAGGGAAAACATCCTCAC

Downstream 100 bases:

>100_bases
ATCCCACCAAAACGTCGACCCCGGCAAAAACGACCTGGAGATAGCTCCCGATGAACATCCATGAATATCAAGGCAAGGCG
CTGCTGAAGAGCTTTGGCGC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MARNKIALIGSGMIGGTLAHMIGLKDLGDVVLFDIAEGIPQGKGLDIAQSSPVDGFDSRLTGVNDYAGIEGADVCIVTAG
VPRKPGMSRDDLLGINLKVMEQVGAGLKKYAPKAFVICITNPLDAMVWALQKFSGLPKTHVVGMAGVLDSARFRYFLAEE
FKVSVEDVTAFVLGGHGDSMVPMIRYSTVSGIPLPDLVKMGWTSKEKLDQIVQRTRDGGAEIVGLLKTGSAYYAPAASAI
AMAESYLKDKKRVLPCAAHLSGQYGVKGTYVGVPVVIGAGGVERIIEIDLNKSEQKMFESSVATVQGLTEACVKIAPQLA
SK

Sequences:

>Translated_322_residues
MARNKIALIGSGMIGGTLAHMIGLKDLGDVVLFDIAEGIPQGKGLDIAQSSPVDGFDSRLTGVNDYAGIEGADVCIVTAG
VPRKPGMSRDDLLGINLKVMEQVGAGLKKYAPKAFVICITNPLDAMVWALQKFSGLPKTHVVGMAGVLDSARFRYFLAEE
FKVSVEDVTAFVLGGHGDSMVPMIRYSTVSGIPLPDLVKMGWTSKEKLDQIVQRTRDGGAEIVGLLKTGSAYYAPAASAI
AMAESYLKDKKRVLPCAAHLSGQYGVKGTYVGVPVVIGAGGVERIIEIDLNKSEQKMFESSVATVQGLTEACVKIAPQLA
SK
>Mature_321_residues
ARNKIALIGSGMIGGTLAHMIGLKDLGDVVLFDIAEGIPQGKGLDIAQSSPVDGFDSRLTGVNDYAGIEGADVCIVTAGV
PRKPGMSRDDLLGINLKVMEQVGAGLKKYAPKAFVICITNPLDAMVWALQKFSGLPKTHVVGMAGVLDSARFRYFLAEEF
KVSVEDVTAFVLGGHGDSMVPMIRYSTVSGIPLPDLVKMGWTSKEKLDQIVQRTRDGGAEIVGLLKTGSAYYAPAASAIA
MAESYLKDKKRVLPCAAHLSGQYGVKGTYVGVPVVIGAGGVERIIEIDLNKSEQKMFESSVATVQGLTEACVKIAPQLAS
K

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=309, Percent_Identity=31.0679611650485, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI221136809, Length=309, Percent_Identity=31.0679611650485, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI9257228, Length=313, Percent_Identity=31.9488817891374, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI4504973, Length=313, Percent_Identity=31.9488817891374, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI5031857, Length=314, Percent_Identity=30.2547770700637, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI260099723, Length=314, Percent_Identity=30.2547770700637, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI15082234, Length=307, Percent_Identity=27.6872964169381, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI291575128, Length=307, Percent_Identity=29.6416938110749, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI4557032, Length=307, Percent_Identity=29.6416938110749, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI260099727, Length=221, Percent_Identity=33.9366515837104, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=34, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI207028494, Length=197, Percent_Identity=30.4568527918782, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI21735621, Length=315, Percent_Identity=26.031746031746, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI103472011, Length=310, Percent_Identity=20.9677419354839, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789632, Length=320, Percent_Identity=29.6875, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535107, Length=249, Percent_Identity=28.9156626506024, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17554310, Length=251, Percent_Identity=34.2629482071713, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322765, Length=297, Percent_Identity=27.9461279461279, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=255, Percent_Identity=27.843137254902, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324446, Length=202, Percent_Identity=26.2376237623762, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI17136226, Length=304, Percent_Identity=27.9605263157895, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45550422, Length=305, Percent_Identity=27.2131147540984, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24647881, Length=243, Percent_Identity=30.8641975308642, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24663595, Length=287, Percent_Identity=26.8292682926829, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24663599, Length=310, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_RHILO (Q98EC4)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105210.1
- ProteinModelPortal:   Q98EC4
- SMR:   Q98EC4
- GeneID:   1227871
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll4308
- NMPDR:   fig|266835.1.peg.3314
- HOGENOM:   HBG566126
- OMA:   SINLKVM
- ProtClustDB:   PRK06223
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 34019; Mature: 33888

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: ACT_SITE 176-176 BINDING 34-34 BINDING 83-83 BINDING 89-89 BINDING 96-96 BINDING 121-121 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNKIALIGSGMIGGTLAHMIGLKDLGDVVLFDIAEGIPQGKGLDIAQSSPVDGFDSRL
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHC
TGVNDYAGIEGADVCIVTAGVPRKPGMSRDDLLGINLKVMEQVGAGLKKYAPKAFVICIT
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKFSGLPKTHVVGMAGVLDSARFRYFLAEEFKVSVEDVTAFVLGGHGDSM
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEHHHHEEEHHHEEEEEECCCCCCC
VPMIRYSTVSGIPLPDLVKMGWTSKEKLDQIVQRTRDGGAEIVGLLKTGSAYYAPAASAI
CEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHH
AMAESYLKDKKRVLPCAAHLSGQYGVKGTYVGVPVVIGAGGVERIIEIDLNKSEQKMFES
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEECCCCEEEEEECCCCHHHHHHHH
SVATVQGLTEACVKIAPQLASK
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARNKIALIGSGMIGGTLAHMIGLKDLGDVVLFDIAEGIPQGKGLDIAQSSPVDGFDSRL
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHC
TGVNDYAGIEGADVCIVTAGVPRKPGMSRDDLLGINLKVMEQVGAGLKKYAPKAFVICIT
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKFSGLPKTHVVGMAGVLDSARFRYFLAEEFKVSVEDVTAFVLGGHGDSM
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEHHHHEEEHHHEEEEEECCCCCCC
VPMIRYSTVSGIPLPDLVKMGWTSKEKLDQIVQRTRDGGAEIVGLLKTGSAYYAPAASAI
CEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHH
AMAESYLKDKKRVLPCAAHLSGQYGVKGTYVGVPVVIGAGGVERIIEIDLNKSEQKMFES
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEECCCCEEEEEECCCCHHHHHHHH
SVATVQGLTEACVKIAPQLASK
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968