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The map label for this gene is sucB [H]

Identifier: 13473635

GI number: 13473635

Start: 3422203

End: 3423477

Strand: Reverse

Name: sucB [H]

Synonym: mll4300

Alternate gene names: 13473635

Gene position: 3423477-3422203 (Counterclockwise)

Preceding gene: 13473636

Following gene: 13473634

Centisome position: 48.66

GC content: 65.8

Gene sequence:

>1275_bases
ATGGCTACCGAAATCCGCGTTCCCACTCTCGGCGAATCCGTCACCGAGGCGACCATCGGCAAATGGTTCAAGAAGGTCGG
CGATGCCATTGCCGTCGACGAGCCGCTGGTCGAGCTCGAAACCGACAAGGTGACGGTGGAAGTTCCGGCCGCCGCCGCCG
GCACGCTGGGCGAAATCGTCGCCAAGGAAGGCGAGACGGTCGGTGTCGGCGCTTTGTTGGGCTCGATCTCGGCTGGTGGC
TCTGCGCCGGCGACCAAGCCGCAGGCGGTGTCGCAGGCCTCGTCGCCTGATGCGGCGTCGACCAGCAAGCAGGCCGCGGC
CGAGACCGCCAAGATCGCCGGCGATGCCGGCGCGGTCGAGCCGCGCAGCATGCCGCCGGCGCCGGCTGCGGCAAAACTGA
TCGCCGAGAACAATCTGTCGGTTGACCAGCTTTCCGGCTCGGGCAAGCGCGGCCAGGTGCTGAAGGGCGACGTGCTCGAC
GCCATCGCCAAGGGCGCGCCGTCGCAGCCGGCCGAGACGCCGAGGGCTGCGCCGGCACCGATCGCGGTGCGTGCGCCATC
CTCCGGCGACGATGCTTCGCGGGAAGAACGCGTGCGCATGACCAAATTGCGCCAGACCATCGCGCGCCGGCTGAAGGAAG
CGCAGTCGACCGCCGCCATGCTGACGACCTTCAACGAGGTCGACATGTCGGCGGTGATGGCGCTGCGGACCAAGTACAAG
GACGTGTTCGAGAAGAAGCATGGCGTGAAGCTCGGCTTCATGGGCTTCTTCACCAAGGCCGTCACCCATGCGTTGAAGGA
AATCCCGGCGGTTAATGCCGAGATCGATGGCACCGACATCATCTACAAGAACTTTGCCCATGTCGGCGTCGCCGTCGGCA
CCGAGAAGGGCCTGGTCGTGCCGGTCGTGCGCGATGCCGACCAGATGTCGATCGCCGAGATCGAGAAGGAGATCGGCCGG
CTCGGCATCGCCGCGCGCGACGGCAAGCTGTCGGTCGCCGACATGCAGGGCGGCACGTTCACGATTTCCAATGGCGGCGT
CTACGGTTCGCTGATGTCGACGCCGATCCTCAACGCGCCGCAGTCGGGCATTCTGGGCATGCACAAGATCCAGGACCGGC
CTGTCGTGGTTGGCGGCCAGATCGTGATCCGGCCGATGATGTATCTGGCGCTCAGCTACGATCACCGCATCGTCGACGGC
AAGGAAGCCGTGACCTTCCTGGTGCGCGTCAAGGAAAGCCTGGAGGATCCGGAACGGCTGGTGCTCGATCTCTAG

Upstream 100 bases:

>100_bases
GATGTCCAAGCACCTTGCCCAACTCGCCGCCTTGCTCGAAGACGCGCTCGGCGAATAAAGCAGGCGAATAGAACAGAACC
GACAAGAACGGACAGGCACA

Downstream 100 bases:

>100_bases
GCCTGCTGCGATGGCCGAGTCTGTCGAGCCAATCGACCTGTTCGAGCTTGTCGCGACAGGCCGCGCGGGTCCGATCGAAC
TCGGCAAGAGCAACCGTGAA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MATEIRVPTLGESVTEATIGKWFKKVGDAIAVDEPLVELETDKVTVEVPAAAAGTLGEIVAKEGETVGVGALLGSISAGG
SAPATKPQAVSQASSPDAASTSKQAAAETAKIAGDAGAVEPRSMPPAPAAAKLIAENNLSVDQLSGSGKRGQVLKGDVLD
AIAKGAPSQPAETPRAAPAPIAVRAPSSGDDASREERVRMTKLRQTIARRLKEAQSTAAMLTTFNEVDMSAVMALRTKYK
DVFEKKHGVKLGFMGFFTKAVTHALKEIPAVNAEIDGTDIIYKNFAHVGVAVGTEKGLVVPVVRDADQMSIAEIEKEIGR
LGIAARDGKLSVADMQGGTFTISNGGVYGSLMSTPILNAPQSGILGMHKIQDRPVVVGGQIVIRPMMYLALSYDHRIVDG
KEAVTFLVRVKESLEDPERLVLDL

Sequences:

>Translated_424_residues
MATEIRVPTLGESVTEATIGKWFKKVGDAIAVDEPLVELETDKVTVEVPAAAAGTLGEIVAKEGETVGVGALLGSISAGG
SAPATKPQAVSQASSPDAASTSKQAAAETAKIAGDAGAVEPRSMPPAPAAAKLIAENNLSVDQLSGSGKRGQVLKGDVLD
AIAKGAPSQPAETPRAAPAPIAVRAPSSGDDASREERVRMTKLRQTIARRLKEAQSTAAMLTTFNEVDMSAVMALRTKYK
DVFEKKHGVKLGFMGFFTKAVTHALKEIPAVNAEIDGTDIIYKNFAHVGVAVGTEKGLVVPVVRDADQMSIAEIEKEIGR
LGIAARDGKLSVADMQGGTFTISNGGVYGSLMSTPILNAPQSGILGMHKIQDRPVVVGGQIVIRPMMYLALSYDHRIVDG
KEAVTFLVRVKESLEDPERLVLDL
>Mature_423_residues
ATEIRVPTLGESVTEATIGKWFKKVGDAIAVDEPLVELETDKVTVEVPAAAAGTLGEIVAKEGETVGVGALLGSISAGGS
APATKPQAVSQASSPDAASTSKQAAAETAKIAGDAGAVEPRSMPPAPAAAKLIAENNLSVDQLSGSGKRGQVLKGDVLDA
IAKGAPSQPAETPRAAPAPIAVRAPSSGDDASREERVRMTKLRQTIARRLKEAQSTAAMLTTFNEVDMSAVMALRTKYKD
VFEKKHGVKLGFMGFFTKAVTHALKEIPAVNAEIDGTDIIYKNFAHVGVAVGTEKGLVVPVVRDADQMSIAEIEKEIGRL
GIAARDGKLSVADMQGGTFTISNGGVYGSLMSTPILNAPQSGILGMHKIQDRPVVVGGQIVIRPMMYLALSYDHRIVDGK
EAVTFLVRVKESLEDPERLVLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=441, Percent_Identity=27.2108843537415, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=29.295154185022, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=29.295154185022, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI31711992, Length=445, Percent_Identity=29.2134831460674, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=36.6459627329193, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786946, Length=424, Percent_Identity=49.2924528301887, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI1786305, Length=426, Percent_Identity=33.0985915492958, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25146366, Length=419, Percent_Identity=42.2434367541766, Blast_Score=317, Evalue=9e-87,
Organism=Caenorhabditis elegans, GI17560088, Length=452, Percent_Identity=32.5221238938053, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=431, Percent_Identity=27.8422273781903, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=29.7385620915033, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=426, Percent_Identity=44.8356807511737, Blast_Score=347, Evalue=3e-96,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=26, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24645909, Length=230, Percent_Identity=56.5217391304348, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI18859875, Length=427, Percent_Identity=30.4449648711944, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=32.7586206896552, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=32.7586206896552, Blast_Score=120, Evalue=3e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44324; Mature: 44193

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEIRVPTLGESVTEATIGKWFKKVGDAIAVDEPLVELETDKVTVEVPAAAAGTLGEIV
CCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCEEEECCEEEEEECCCCCCHHHHHH
AKEGETVGVGALLGSISAGGSAPATKPQAVSQASSPDAASTSKQAAAETAKIAGDAGAVE
HCCCCEEEHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
PRSMPPAPAAAKLIAENNLSVDQLSGSGKRGQVLKGDVLDAIAKGAPSQPAETPRAAPAP
CCCCCCCCHHHHHHHCCCCCHHHCCCCCCCCCEECCHHHHHHHCCCCCCCCCCCCCCCCC
IAVRAPSSGDDASREERVRMTKLRQTIARRLKEAQSTAAMLTTFNEVDMSAVMALRTKYK
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVFEKKHGVKLGFMGFFTKAVTHALKEIPAVNAEIDGTDIIYKNFAHVGVAVGTEKGLVV
HHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEECCCCEEE
PVVRDADQMSIAEIEKEIGRLGIAARDGKLSVADMQGGTFTISNGGVYGSLMSTPILNAP
EEECCCCHHHHHHHHHHHHHHCEEECCCCEEEEECCCCEEEEECCCEEEHHHCCCCCCCC
QSGILGMHKIQDRPVVVGGQIVIRPMMYLALSYDHRIVDGKEAVTFLVRVKESLEDPERL
CCCCCCHHHCCCCCEEECCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCHHH
VLDL
EECC
>Mature Secondary Structure 
ATEIRVPTLGESVTEATIGKWFKKVGDAIAVDEPLVELETDKVTVEVPAAAAGTLGEIV
CCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCEEEECCEEEEEECCCCCCHHHHHH
AKEGETVGVGALLGSISAGGSAPATKPQAVSQASSPDAASTSKQAAAETAKIAGDAGAVE
HCCCCEEEHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
PRSMPPAPAAAKLIAENNLSVDQLSGSGKRGQVLKGDVLDAIAKGAPSQPAETPRAAPAP
CCCCCCCCHHHHHHHCCCCCHHHCCCCCCCCCEECCHHHHHHHCCCCCCCCCCCCCCCCC
IAVRAPSSGDDASREERVRMTKLRQTIARRLKEAQSTAAMLTTFNEVDMSAVMALRTKYK
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVFEKKHGVKLGFMGFFTKAVTHALKEIPAVNAEIDGTDIIYKNFAHVGVAVGTEKGLVV
HHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEECCCCEEE
PVVRDADQMSIAEIEKEIGRLGIAARDGKLSVADMQGGTFTISNGGVYGSLMSTPILNAP
EEECCCCHHHHHHHHHHHHHHCEEECCCCEEEEECCCCEEEEECCCEEEHHHCCCCCCCC
QSGILGMHKIQDRPVVVGGQIVIRPMMYLALSYDHRIVDGKEAVTFLVRVKESLEDPERL
CCCCCCHHHCCCCCEEECCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCHHH
VLDL
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]