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The map label for this gene is dfrA [H]

Identifier: 13471865

GI number: 13471865

Start: 1623907

End: 1624974

Strand: Reverse

Name: dfrA [H]

Synonym: mll1975

Alternate gene names: 13471865

Gene position: 1624974-1623907 (Counterclockwise)

Preceding gene: 13471867

Following gene: 13471863

Centisome position: 23.09

GC content: 66.95

Gene sequence:

>1068_bases
ATGGCCAATGAACATCAGGAGACTGACATGAACGACGAACTGGTGCTGGTGACCGGAGGCTCAGGCTTCATTGCATCGCA
CTGCATGCTGAAGCTGCTCGACGCCGGTTATCGCCTGCGCACGACGGTGCGTTCGCTCGAGCGCGAAGCCGAGGTGCGCG
CGATGCTCAGGGAAGGCGGCGCCGAGCCCGGCGATCGGCTGTCCTTCGTCGCCGCCGACCTGACCGCGGACGCCGGCTGG
GCGGAGGCGGTCGCCGGCTGCGCCTATGTCATGCATGGCGCATCGCCGACGCCGTCCGGCAGCCAGACGCGCGAGGAGGA
TTGGGTGAGGCCCGCCGTCGACGGCGTGCTGCGGGTGCTCAAGGCCGCGCGCGATGCCGGCATAAAACGCGTGGTGCTGA
CTTCGGCCATCGGTGCTGTCGCTATGGGGCATGCGCCGCAGACACGGCCGTTCAACGAGACCGACTGGTCCGACCTCAGC
GGTGCCGTCGCGCCCTACCAGAGATCGAAGACGCTGTCGGAGCGCGCCGCCTGGGATTTCATCGCTCGCGAGGGCGGCGG
CCTCGAACTCTCCGTCGTCAATCCGGTCGCCGTGCTCGGCCCGGTGCTCGCCGCTGATTTCTCGCATTCGATCGGGCTGA
TCAAGCGGCTGATGGACGGCATGCCCGGTTGTCCCCGGGTCAACTCAGGCTATGTCGACGTGCGCGACCTGGCCGATCTG
CATCTGCTGGCGATGACCAGTCCTGCGGCCAAGGGCGAGCGCTTCATCGGTATTTCGGGCCACAGCCTGTGGATGGCGGA
TGTTGCGAAAGTGCTGCGGCGCAGGATGGGCGCGGCAGCAGCCAAGGTGCCGACCAGGGAGATTCCCAATTGGATGATCC
GCCTGCTGGCGCTGCGTGGCGATCCGACGACGAAGATGCTGGCCAGGCATCTCGGCGTGATGATGGACGCCACCAGCGAA
AAGGCGACGCGTCTGCTTGGCTGGACGCCGCGCCCGGCCGAGGAGGCTATCGTCGCCACGGCCGAGAGTTTGTTGCGGCT
GGGGTTGGTGGGTGGGTCAAAAGCGTAG

Upstream 100 bases:

>100_bases
TTTGAGGATGTCGGAGAAAGGATCGTCGGTCATTTTCGTACTTTCGCGCAACTTATGCGGATTTTCAAGCATTCACAATC
CGATCTGGTGCGCCTAGCTG

Downstream 100 bases:

>100_bases
GCGGGTGGTTCGCCTCAATCCCTCCGAGACATCCCGACCCAGTCAGCCGCCACCTCGGCAAACAGCCAGTCACGGAACGC
CCTGATCTTCTTCTGCCGCA

Product: cinnamoyl-CoA reductase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MANEHQETDMNDELVLVTGGSGFIASHCMLKLLDAGYRLRTTVRSLEREAEVRAMLREGGAEPGDRLSFVAADLTADAGW
AEAVAGCAYVMHGASPTPSGSQTREEDWVRPAVDGVLRVLKAARDAGIKRVVLTSAIGAVAMGHAPQTRPFNETDWSDLS
GAVAPYQRSKTLSERAAWDFIAREGGGLELSVVNPVAVLGPVLAADFSHSIGLIKRLMDGMPGCPRVNSGYVDVRDLADL
HLLAMTSPAAKGERFIGISGHSLWMADVAKVLRRRMGAAAAKVPTREIPNWMIRLLALRGDPTTKMLARHLGVMMDATSE
KATRLLGWTPRPAEEAIVATAESLLRLGLVGGSKA

Sequences:

>Translated_355_residues
MANEHQETDMNDELVLVTGGSGFIASHCMLKLLDAGYRLRTTVRSLEREAEVRAMLREGGAEPGDRLSFVAADLTADAGW
AEAVAGCAYVMHGASPTPSGSQTREEDWVRPAVDGVLRVLKAARDAGIKRVVLTSAIGAVAMGHAPQTRPFNETDWSDLS
GAVAPYQRSKTLSERAAWDFIAREGGGLELSVVNPVAVLGPVLAADFSHSIGLIKRLMDGMPGCPRVNSGYVDVRDLADL
HLLAMTSPAAKGERFIGISGHSLWMADVAKVLRRRMGAAAAKVPTREIPNWMIRLLALRGDPTTKMLARHLGVMMDATSE
KATRLLGWTPRPAEEAIVATAESLLRLGLVGGSKA
>Mature_354_residues
ANEHQETDMNDELVLVTGGSGFIASHCMLKLLDAGYRLRTTVRSLEREAEVRAMLREGGAEPGDRLSFVAADLTADAGWA
EAVAGCAYVMHGASPTPSGSQTREEDWVRPAVDGVLRVLKAARDAGIKRVVLTSAIGAVAMGHAPQTRPFNETDWSDLSG
AVAPYQRSKTLSERAAWDFIAREGGGLELSVVNPVAVLGPVLAADFSHSIGLIKRLMDGMPGCPRVNSGYVDVRDLADLH
LLAMTSPAAKGERFIGISGHSLWMADVAKVLRRRMGAAAAKVPTREIPNWMIRLLALRGDPTTKMLARHLGVMMDATSEK
ATRLLGWTPRPAEEAIVATAESLLRLGLVGGSKA

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71987463, Length=345, Percent_Identity=41.1594202898551, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6324421, Length=357, Percent_Identity=29.4117647058824, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321399, Length=359, Percent_Identity=27.5766016713092, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321281, Length=363, Percent_Identity=27.2727272727273, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320750, Length=357, Percent_Identity=26.3305322128852, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 37939; Mature: 37808

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANEHQETDMNDELVLVTGGSGFIASHCMLKLLDAGYRLRTTVRSLEREAEVRAMLREGG
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
AEPGDRLSFVAADLTADAGWAEAVAGCAYVMHGASPTPSGSQTREEDWVRPAVDGVLRVL
CCCCCHHEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KAARDAGIKRVVLTSAIGAVAMGHAPQTRPFNETDWSDLSGAVAPYQRSKTLSERAAWDF
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHH
IAREGGGLELSVVNPVAVLGPVLAADFSHSIGLIKRLMDGMPGCPRVNSGYVDVRDLADL
HHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHH
HLLAMTSPAAKGERFIGISGHSLWMADVAKVLRRRMGAAAAKVPTREIPNWMIRLLALRG
HHHEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
DPTTKMLARHLGVMMDATSEKATRLLGWTPRPAEEAIVATAESLLRLGLVGGSKA
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ANEHQETDMNDELVLVTGGSGFIASHCMLKLLDAGYRLRTTVRSLEREAEVRAMLREGG
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
AEPGDRLSFVAADLTADAGWAEAVAGCAYVMHGASPTPSGSQTREEDWVRPAVDGVLRVL
CCCCCHHEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KAARDAGIKRVVLTSAIGAVAMGHAPQTRPFNETDWSDLSGAVAPYQRSKTLSERAAWDF
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHH
IAREGGGLELSVVNPVAVLGPVLAADFSHSIGLIKRLMDGMPGCPRVNSGYVDVRDLADL
HHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHH
HLLAMTSPAAKGERFIGISGHSLWMADVAKVLRRRMGAAAAKVPTREIPNWMIRLLALRG
HHHEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
DPTTKMLARHLGVMMDATSEKATRLLGWTPRPAEEAIVATAESLLRLGLVGGSKA
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]