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The map label for this gene is cobD [H]

Identifier: 13471358

GI number: 13471358

Start: 1097759

End: 1098733

Strand: Direct

Name: cobD [H]

Synonym: mlr1304

Alternate gene names: 13471358

Gene position: 1097759-1098733 (Clockwise)

Preceding gene: 13471355

Following gene: 13471363

Centisome position: 15.6

GC content: 67.28

Gene sequence:

>975_bases
ATGTCAGTCCTGATCGCTTTCCTGTCATTGGCCGTCGAATTCGTCCTGGGTTACCCGGACTGGCTGTTCCGCGCCATCGG
CCATCCGGTGACGTGGTTCGGCAGGCTGATATCCTTTCTCGACTTGAGGCTCAACCGCGCCACTGATCCCGACGCGCTGC
GTCGCCAACGCGGCGTCCAGGCGCTGCTGGTCATCGTGCTGGTGCCGGCGGTGATCGGCCTCTTCGTCCAGCTTGTCCTG
CTCTGGTTCGTGCCGCTGGGGTTCTTCATTGCCGCGCTGCTGGCGACGTCGCTGCTGTCGCAGAAGAGCCTTTACGAACA
TGTCGAGGCCGTAGCTGACTCGCTCGATTCGGGCGGCCTCGCCATGGGCCGTGTCGCCGTCTCGCGCATCGTCGGCCGCG
ATCCCGAGGGGCTCGACCGTGCTGCCGTCTGCCGCGCGGCGATCGAGAGCCTGGCCGAGAATTTTTCCGACGGCATCGTC
GCCCCCGCCTTCTGGACCGGTGTTGGCGGACTCGCCGGCGGCGCTGCCTACAAGGCCGCCAACACCGCCGATTCGATGAT
CGGCCACCGCACCCCCCGCCACGAGGCTTTCGGCTGGGCGGCGGCGCGCTTCGACGACTGGATCAACCTGCCGGCATCGC
GGCTGACGGCGCTGCTGATCGTGCTGGCGGCTTTCCTGGTCAAGGACGCCGATCCCCGCAGTGCCTGGCAGGCGGTGTCG
CGCGATGCCAAAAAACACCGCTCGCCCAATGCCGGCTGGCCGGAAGCGGCCATGGCCGGCGCGCTTGGCCTGGCGCTTGC
CGGGCCGCGCAGCTATGGCGGCATCGTGGTCGATGATGTCTTTATGGGCGAAGGCGGCCGCCGCGACGTCGACAGCGGCG
ACATCAGGCGGGCGCTGACGCTCTACCGCGTCGCTGACTACCTGCTGATCGCGTTGTTCGCCGTGGTTTCGGTCATCGCC
GTTCTTGCGTTCTAG

Upstream 100 bases:

>100_bases
ACCGGTCGAAAGGTCGACGAAAGGCTGAAGAGCGTTGGGGAAAAGCGCCCTCGCCCGGCCAAGACTTCCACCATGGTCCA
CCGCTCCTTCAAGAAGCTTC

Downstream 100 bases:

>100_bases
AACTCGATCCCCTGCTGCGCCTTCACGCCTGCCGAGAAGTGGTGTTTGGTCACGCCCATCTCGGTGACCAGATCGGCCGC
CTCGACCAGCGCCGGCTTGG

Product: cobalamin biosynthetic protein cobD

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSVLIAFLSLAVEFVLGYPDWLFRAIGHPVTWFGRLISFLDLRLNRATDPDALRRQRGVQALLVIVLVPAVIGLFVQLVL
LWFVPLGFFIAALLATSLLSQKSLYEHVEAVADSLDSGGLAMGRVAVSRIVGRDPEGLDRAAVCRAAIESLAENFSDGIV
APAFWTGVGGLAGGAAYKAANTADSMIGHRTPRHEAFGWAAARFDDWINLPASRLTALLIVLAAFLVKDADPRSAWQAVS
RDAKKHRSPNAGWPEAAMAGALGLALAGPRSYGGIVVDDVFMGEGGRRDVDSGDIRRALTLYRVADYLLIALFAVVSVIA
VLAF

Sequences:

>Translated_324_residues
MSVLIAFLSLAVEFVLGYPDWLFRAIGHPVTWFGRLISFLDLRLNRATDPDALRRQRGVQALLVIVLVPAVIGLFVQLVL
LWFVPLGFFIAALLATSLLSQKSLYEHVEAVADSLDSGGLAMGRVAVSRIVGRDPEGLDRAAVCRAAIESLAENFSDGIV
APAFWTGVGGLAGGAAYKAANTADSMIGHRTPRHEAFGWAAARFDDWINLPASRLTALLIVLAAFLVKDADPRSAWQAVS
RDAKKHRSPNAGWPEAAMAGALGLALAGPRSYGGIVVDDVFMGEGGRRDVDSGDIRRALTLYRVADYLLIALFAVVSVIA
VLAF
>Mature_323_residues
SVLIAFLSLAVEFVLGYPDWLFRAIGHPVTWFGRLISFLDLRLNRATDPDALRRQRGVQALLVIVLVPAVIGLFVQLVLL
WFVPLGFFIAALLATSLLSQKSLYEHVEAVADSLDSGGLAMGRVAVSRIVGRDPEGLDRAAVCRAAIESLAENFSDGIVA
PAFWTGVGGLAGGAAYKAANTADSMIGHRTPRHEAFGWAAARFDDWINLPASRLTALLIVLAAFLVKDADPRSAWQAVSR
DAKKHRSPNAGWPEAAMAGALGLALAGPRSYGGIVVDDVFMGEGGRRDVDSGDIRRALTLYRVADYLLIALFAVVSVIAV
LAF

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 34695; Mature: 34563

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVLIAFLSLAVEFVLGYPDWLFRAIGHPVTWFGRLISFLDLRLNRATDPDALRRQRGVQ
HHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ALLVIVLVPAVIGLFVQLVLLWFVPLGFFIAALLATSLLSQKSLYEHVEAVADSLDSGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AMGRVAVSRIVGRDPEGLDRAAVCRAAIESLAENFSDGIVAPAFWTGVGGLAGGAAYKAA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHCCCHHHHHC
NTADSMIGHRTPRHEAFGWAAARFDDWINLPASRLTALLIVLAAFLVKDADPRSAWQAVS
CHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RDAKKHRSPNAGWPEAAMAGALGLALAGPRSYGGIVVDDVFMGEGGRRDVDSGDIRRALT
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCCCCCCHHHHHHHH
LYRVADYLLIALFAVVSVIAVLAF
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2211520 [H]