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The map label for this gene is lcrF [H]

Identifier: 13471327

GI number: 13471327

Start: 1066546

End: 1067175

Strand: Direct

Name: lcrF [H]

Synonym: mlr1270

Alternate gene names: 13471327

Gene position: 1066546-1067175 (Clockwise)

Preceding gene: 13471313

Following gene: 13471328

Centisome position: 15.16

GC content: 61.43

Gene sequence:

>630_bases
GTGCCCGCCGGGCTCGATGGCGACTGGAAGGATGACGCGGACTGCACCATCCTGCGGCTGTGGGTCAGTCCGGCGCTGGT
TCGGCGCGCTGCCGAGGACCTCGATATCGATCCGGACAGGGTCGTCGTCAATCCGCAGTTCCAGCGCCGCGATGCCAGGA
TCGAACACATCGCCCATGCGCTGGCGACCGGCCTGAACCCGGACGTGCCATCCGACCGGCTGTATTTCGAAAGCCTGGCC
AAAGCACTGGCTGTCCAGATCATCCAGGGCGCTGCGTCAAAACCGGAAGCTCCCGCCAGGCAAACGCTTTCTTTCGGCCA
GAAGCGCAGGCTGACCGATTTCATCGAAGCCAATCTCGACCAGGATCTTTCGCTGGATGAGATGGCGCAAGTGGCAGCCA
TCAGCGTCTCGCATCTGAAAGTGCTGTTTCGCCACACCTTCGGCATGCCTCCCCACCAATATGTCATCCATCGCCGCGTC
GATCGGGCCAGGGCTCTGTTGCATCAGGGAAAAATGCCGCTTAGCCAGATTGCGCTGGAAGCAGGCTTTTCGCATCAGAG
CCATATGGCGCAAAGCATGAAGCGCCTCCTCGGCGTCACGCCGGCAACACTGATCCGCTTGCAGCGGTAA

Upstream 100 bases:

>100_bases
CCATCGGCTTGGGATCCACGTCGGAGCGCCGGTCAACGCCTCGTGCCACTGCGACGGCAAACATCACCGCCGGGTCCAGT
CGGACGGCGACATCGATATT

Downstream 100 bases:

>100_bases
ACTACGGTCTGGCATTTTCGCCGTGCTTTGGCTTTCGAAGCCAATCATCCGTTCTTGCGCTCCACCATCCAAATCTGAGC
GACCCGAGCCCGCCGATTTG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MPAGLDGDWKDDADCTILRLWVSPALVRRAAEDLDIDPDRVVVNPQFQRRDARIEHIAHALATGLNPDVPSDRLYFESLA
KALAVQIIQGAASKPEAPARQTLSFGQKRRLTDFIEANLDQDLSLDEMAQVAAISVSHLKVLFRHTFGMPPHQYVIHRRV
DRARALLHQGKMPLSQIALEAGFSHQSHMAQSMKRLLGVTPATLIRLQR

Sequences:

>Translated_209_residues
MPAGLDGDWKDDADCTILRLWVSPALVRRAAEDLDIDPDRVVVNPQFQRRDARIEHIAHALATGLNPDVPSDRLYFESLA
KALAVQIIQGAASKPEAPARQTLSFGQKRRLTDFIEANLDQDLSLDEMAQVAAISVSHLKVLFRHTFGMPPHQYVIHRRV
DRARALLHQGKMPLSQIALEAGFSHQSHMAQSMKRLLGVTPATLIRLQR
>Mature_208_residues
PAGLDGDWKDDADCTILRLWVSPALVRRAAEDLDIDPDRVVVNPQFQRRDARIEHIAHALATGLNPDVPSDRLYFESLAK
ALAVQIIQGAASKPEAPARQTLSFGQKRRLTDFIEANLDQDLSLDEMAQVAAISVSHLKVLFRHTFGMPPHQYVIHRRVD
RARALLHQGKMPLSQIALEAGFSHQSHMAQSMKRLLGVTPATLIRLQR

Specific function: Transcriptional activator of the thermally regulated virulent yopE gene. LcrF activity could be modulated by the interaction with an inducer molecule serving as a temperature messenger. The availability of the messenger would in turn be controlled by a te

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR014710 [H]

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 23375; Mature: 23244

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAGLDGDWKDDADCTILRLWVSPALVRRAAEDLDIDPDRVVVNPQFQRRDARIEHIAHA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHH
LATGLNPDVPSDRLYFESLAKALAVQIIQGAASKPEAPARQTLSFGQKRRLTDFIEANLD
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
QDLSLDEMAQVAAISVSHLKVLFRHTFGMPPHQYVIHRRVDRARALLHQGKMPLSQIALE
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AGFSHQSHMAQSMKRLLGVTPATLIRLQR
HCCCHHHHHHHHHHHHHCCCHHHHHEECC
>Mature Secondary Structure 
PAGLDGDWKDDADCTILRLWVSPALVRRAAEDLDIDPDRVVVNPQFQRRDARIEHIAHA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHH
LATGLNPDVPSDRLYFESLAKALAVQIIQGAASKPEAPARQTLSFGQKRRLTDFIEANLD
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
QDLSLDEMAQVAAISVSHLKVLFRHTFGMPPHQYVIHRRVDRARALLHQGKMPLSQIALE
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AGFSHQSHMAQSMKRLLGVTPATLIRLQR
HCCCHHHHHHHHHHHHHCCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1624422; 9746557; 9748454; 11586360 [H]